FastQCFastQC Report
Thu 26 May 2016
SRR1513039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755997
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC47390.26987517632433317No Hit
CCCATGTACTCTGCGTTGATACCAC36430.2074604911056226No Hit
GTATCAACGCAGAGTACTTTTTTTT23130.13172004280189545No Hit
GTCCTACAGTGGACATTTCTAAATT20580.11719837790155677No Hit
GTCCTACAGTGTGCATTTCTCATTT19660.11195918899633654No Hit
GAGTACATGGGAAGCAGTGGTATCA18820.10717558173504853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7450.013.2634154
TGCACCG751.47779265E-512.6675415
TCCAACG1501.8189894E-1212.66645818
CCGTGTA604.0970024E-412.6650149
TAGGACA5700.012.16824
GTAGGAC13800.012.1171243
CACCGAC550.00307237312.0889877
ATCCCGT951.0416552E-611.99843510
CGTTTCC951.0420135E-611.99809414
GGCGAGG4200.011.98789819
TGTAGGA15300.011.7371072
CCAACGA907.4633554E-611.6109219
CTGTAGG15000.011.5425911
GTCCTAC13750.011.4157491
TGGCGAG11000.011.14072618
GTAGCAC1803.6379788E-1211.08441453
TTGCGAG600.00587689511.0831518
TATGGCG11800.010.94834916
ATGGCGA12050.010.72120417
TCCTACA15450.010.7006832
Produced by FastQC (version 0.11.5)