##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513039_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1755997
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.219579532311275	31.0	30.0	34.0	24.0	34.0
2	28.906875125640877	31.0	30.0	34.0	16.0	34.0
3	29.283362670892945	31.0	30.0	34.0	23.0	34.0
4	32.14382598603528	35.0	33.0	37.0	19.0	37.0
5	32.39904567035137	35.0	33.0	37.0	22.0	37.0
6	32.84902081267793	37.0	33.0	37.0	26.0	37.0
7	32.83777307136629	37.0	35.0	37.0	26.0	37.0
8	32.820492290134894	37.0	35.0	37.0	26.0	37.0
9	34.188997475508216	39.0	35.0	39.0	25.0	39.0
10	33.705332070612876	39.0	34.0	39.0	19.0	39.0
11	34.0652871274837	39.0	34.0	39.0	25.0	39.0
12	33.89143831111329	39.0	34.0	39.0	23.0	39.0
13	33.98562469070277	39.0	35.0	39.0	25.0	39.0
14	34.279722573557926	39.0	33.0	41.0	17.0	41.0
15	34.8444006453314	40.0	34.0	41.0	22.0	41.0
16	34.44170177967275	39.0	34.0	41.0	17.0	41.0
17	33.99828929092703	39.0	33.0	41.0	11.0	41.0
18	33.43562204263447	39.0	32.0	41.0	10.0	41.0
19	34.30730291680453	39.0	34.0	41.0	18.0	41.0
20	34.55908808500242	39.0	34.0	41.0	18.0	41.0
21	34.57802319707835	40.0	34.0	41.0	18.0	41.0
22	34.1589302259628	40.0	34.0	41.0	10.0	41.0
23	32.53221958807447	39.0	31.0	41.0	2.0	41.0
24	33.6159782733114	39.0	33.0	41.0	2.0	41.0
25	33.338860487802656	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7882633023311527
1101	2	1.014204921065879
1101	3	0.8243150221952256
1101	4	0.7680902419802962
1101	5	1.139988767980931
1101	6	0.7838842734824425
1101	7	0.85377588041586
1101	8	0.883217897620959
1101	9	1.0049165232275215
1101	10	1.3537922261729989
1101	11	1.0303191011656168
1101	12	1.0891933078692375
1101	13	0.8358554127218838
1101	14	-0.005511000379208042
1101	15	0.6365394331087373
1101	16	1.2336960669533568
1101	17	1.754365872472988
1101	18	-3.870290157021678
1101	19	-0.4730791680073594
1101	20	0.31262051434978133
1101	21	0.7460899469071762
1101	22	1.3630789536738774
1101	23	3.1442188170483902
1101	24	2.0579763350071403
1101	25	2.334928760028035
1102	1	0.8852388249775807
1102	2	1.147501160294322
1102	3	0.9534690863775381
1102	4	1.4518695368118486
1102	5	1.2965883085847096
1102	6	0.8505977833456697
1102	7	0.7778732571968519
1102	8	0.700981812665951
1102	9	0.9432493572171339
1102	10	1.270759687985091
1102	11	0.8925341185713123
1102	12	0.9785363617603124
1102	13	0.7997974460495101
1102	14	1.7450073659583651
1102	15	1.1537430131654247
1102	16	1.5121800594204018
1102	17	1.2588296486528634
1102	18	1.6264123910579897
1102	19	1.2739568703422322
1102	20	1.4124500241369233
1102	21	1.2693183467245603
1102	22	1.7161119912055014
1102	23	3.069111577040019
1102	24	1.9512594486292372
1102	25	2.3834435691360696
1103	1	0.7249899029627933
1103	2	0.9513711570693246
1103	3	0.7654577152759039
1103	4	1.2125580140158974
1103	5	1.090144883398679
1103	6	0.7976188413836809
1103	7	0.6526633337606782
1103	8	0.7011460486401973
1103	9	0.7670343096185661
1103	10	1.0276904460983474
1103	11	0.7284080331208287
1103	12	0.8115949731003553
1103	13	0.731521864056937
1103	14	1.49292425658318
1103	15	1.0582520371856887
1103	16	1.2748459377543924
1103	17	1.6691860705780712
1103	18	1.6947023063719442
1103	19	1.0959453797962553
1103	20	1.0469336065117147
1103	21	0.995740033039823
1103	22	1.418342017124921
1103	23	2.7759766407635738
1103	24	1.6289496338874159
1103	25	1.863287933154652
1104	1	0.47328624510916484
1104	2	0.8375326213897765
1104	3	0.5525270111169718
1104	4	1.0946548690281332
1104	5	0.9584518365628227
1104	6	0.5756289808006656
1104	7	0.5283346280100929
1104	8	0.5348124500314171
1104	9	0.6085911817263252
1104	10	0.9711882502071703
1104	11	0.5799186294010781
1104	12	0.6429873744326287
1104	13	0.46351260889143475
1104	14	1.394977193771851
1104	15	0.8052750571992462
1104	16	1.1447121210302527
1104	17	1.5998357006661976
1104	18	2.1375722919291746
1104	19	1.3112945117560528
1104	20	0.7607176133499252
1104	21	0.8707510861833327
1104	22	1.2376039618886807
1104	23	2.607604842490815
1104	24	1.583127722135714
1104	25	1.7052604034585386
1105	1	0.503112917322941
1105	2	0.9193848818119861
1105	3	0.6575024477705007
1105	4	0.7714092022227845
1105	5	0.8170288584908079
1105	6	0.5540745536724074
1105	7	0.357555950357451
1105	8	0.3674314724551664
1105	9	0.47215567192635177
1105	10	0.9327927462635515
1105	11	0.6306446578507803
1105	12	0.5588627326632007
1105	13	0.498855705714071
1105	14	1.2435088289557825
1105	15	0.7180753946644032
1105	16	1.1701502966310002
1105	17	1.4800832620890603
1105	18	1.3882529481307486
1105	19	0.9010603880565142
1105	20	0.8407257020765897
1105	21	0.8325680925137036
1105	22	1.1996865335819038
1105	23	2.558217077922045
1105	24	1.651366713567171
1105	25	1.6202201990080027
1106	1	-0.6286692972227534
1106	2	-0.2216451407335498
1106	3	-0.41683771473810083
1106	4	-2.700516802444259
1106	5	-0.551707999269631
1106	6	-0.6167846875768106
1106	7	-0.5560484529316838
1106	8	-0.470279719194167
1106	9	-0.4327819275247293
1106	10	-0.4828290853955721
1106	11	-0.43557533901679335
1106	12	-0.3983384797680358
1106	13	-0.4270998253124745
1106	14	0.23491719152577417
1106	15	-0.27399879019319684
1106	16	-1.369855269192911
1106	17	-6.043023900787038
1106	18	-6.896220611354032
1106	19	-2.89956714908395
1106	20	-1.7312650714661828
1106	21	-1.3606885447605563
1106	22	-1.6305817212895448
1106	23	-5.226003270209564
1106	24	-2.3161659315401906
1106	25	-1.001649043321919
1107	1	0.5148900552534492
1107	2	0.8849855983458177
1107	3	0.6434562954231566
1107	4	1.017957462105393
1107	5	0.959205834012991
1107	6	0.5724588216889472
1107	7	0.5878035442194971
1107	8	0.594273503325141
1107	9	0.6092182492519242
1107	10	1.0328832140096722
1107	11	0.6828284698400537
1107	12	0.7344305564422768
1107	13	0.6425068296484113
1107	14	1.4634789151887446
1107	15	0.9039524024542587
1107	16	-5.800133737740552
1107	17	-0.8379343764465403
1107	18	0.801332675190146
1107	19	0.8839596470262805
1107	20	0.7127552317850956
1107	21	0.8804056233664994
1107	22	0.9357300605907355
1107	23	2.7367692392086056
1107	24	1.6846107241969221
1107	25	1.3247326333252616
1108	1	0.1985917855763013
1108	2	0.5420653708013283
1108	3	0.30341279556383327
1108	4	0.8176383019450242
1108	5	0.6064239531661215
1108	6	0.11741050805034803
1108	7	0.12050317318481518
1108	8	0.23253447610420608
1108	9	0.20688411582011668
1108	10	0.7398564898414293
1108	11	0.18003801650953477
1108	12	0.23786504508263562
1108	13	0.1172707566372253
1108	14	0.7992867288976839
1108	15	0.5509188422798132
1108	16	0.6387059875882812
1108	17	0.744734589404203
1108	18	0.5897379514439294
1108	19	0.5813603161196212
1108	20	0.4434902233444902
1108	21	0.4106071426743725
1108	22	0.7917881688414212
1108	23	2.4275218373305734
1108	24	1.1573480281288298
1108	25	1.3448493949486746
1109	1	0.09087457588540815
1109	2	0.37397587623157236
1109	3	0.28126758879691494
1109	4	0.28646662274702805
1109	5	0.11117541515442042
1109	6	0.06267732091584577
1109	7	0.03485937619233681
1109	8	-0.04370299256747501
1109	9	0.09184391114175128
1109	10	0.43862815859665005
1109	11	0.052675338982382414
1109	12	0.2041218328854768
1109	13	-0.08075033776545126
1109	14	-0.9969421244857699
1109	15	-0.3079862329081564
1109	16	-0.45051445936243084
1109	17	0.5614443325119751
1109	18	1.2597658858633096
1109	19	0.5144939314078414
1109	20	0.2715630094764876
1109	21	0.19522493716058875
1109	22	0.1726752824587976
1109	23	-4.337587550722894
1109	24	-1.6336659651877952
1109	25	-0.29993966102459524
1110	1	0.4121549565094149
1110	2	0.7405884577817865
1110	3	0.5726870695797963
1110	4	1.0636127032662444
1110	5	0.704449001335675
1110	6	0.3389001515956238
1110	7	0.3332190760199438
1110	8	0.2622877791595286
1110	9	0.20619167844053976
1110	10	0.5986229191009897
1110	11	0.28850123561220187
1110	12	0.5224740115015791
1110	13	0.2828622099815661
1110	14	1.0119058625338013
1110	15	0.46929294770233554
1110	16	0.8823794323997305
1110	17	1.1347381161039038
1110	18	1.8393745293409864
1110	19	1.064653651517368
1110	20	0.6742267014617482
1110	21	0.4658300989088886
1110	22	0.6664914099644648
1110	23	2.077004444679872
1110	24	1.1874453994473058
1110	25	1.4026872699830832
1111	1	0.34356587950835404
1111	2	0.6499528125878591
1111	3	0.41036943941769266
1111	4	0.8771030387599978
1111	5	0.6591425866317735
1111	6	0.19426866695808087
1111	7	0.15215782124035826
1111	8	-0.11174717827533698
1111	9	-0.0047140587257743505
1111	10	0.3091430042938299
1111	11	0.16244858042835375
1111	12	0.24044625394655128
1111	13	0.19118199291087734
1111	14	-0.5168898759467027
1111	15	-0.15945223099611638
1111	16	0.3691215050222496
1111	17	0.9623454259167232
1111	18	1.526834243181483
1111	19	0.5845439323575263
1111	20	0.32815424999810006
1111	21	0.30832972864127584
1111	22	0.5694849454048097
1111	23	0.6544017188844222
1111	24	0.44667810064864
1111	25	0.06111565605434066
1112	1	0.24720848577750942
1112	2	0.6759289690575336
1112	3	0.4639646483471971
1112	4	0.9028244186452241
1112	5	0.6671047043398275
1112	6	0.06792400570292756
1112	7	0.1457903126885043
1112	8	0.07784383224102953
1112	9	0.07764409234593472
1112	10	0.3614495901097001
1112	11	0.1511752566168454
1112	12	0.0654409169806911
1112	13	0.18317870432333905
1112	14	0.5372467808762238
1112	15	0.18124035468759558
1112	16	0.527905769613767
1112	17	0.7510045005376753
1112	18	0.7671283354144194
1112	19	0.24239422792378207
1112	20	0.3090146197227597
1112	21	0.276251005853446
1112	22	0.21250622719152545
1112	23	1.8672256854191076
1112	24	0.8451959489873175
1112	25	0.7576595894490055
1113	1	0.6653080554175723
1113	2	1.1474225136584728
1113	3	0.7838443431828779
1113	4	0.8401581432148646
1113	5	0.982118397610563
1113	6	0.5812993969392366
1113	7	0.5763067446133903
1113	8	0.36373487292960505
1113	9	0.6343146713756695
1113	10	0.9062999422223754
1113	11	0.5800876196841358
1113	12	0.624571394288985
1113	13	0.5144433756848414
1113	14	1.2270893082627623
1113	15	0.8096490010099728
1113	16	1.0753627336200253
1113	17	1.5096899465470628
1113	18	2.0368857689042486
1113	19	1.1890736097704178
1113	20	0.9759306995349704
1113	21	0.9227103172775486
1113	22	0.9922842005874557
1113	23	1.07357838494962
1113	24	1.0535781868589922
1113	25	1.4460398711391491
1114	1	-0.34661158236248113
1114	2	-3.0686545504834584
1114	3	-0.9831517758891088
1114	4	0.11531522839625552
1114	5	0.4541721619685788
1114	6	0.2566601242223001
1114	7	0.1380092352309248
1114	8	0.14782271268850167
1114	9	0.3310151717737142
1114	10	0.39377115511899774
1114	11	0.17993977480752932
1114	12	0.330580080175487
1114	13	0.21912074688921024
1114	14	0.8729592474189118
1114	15	0.47916203694765613
1114	16	0.7178453902787325
1114	17	0.9480983395869771
1114	18	1.1385888281559247
1114	19	0.6077450671797209
1114	20	0.48264449175605506
1114	21	0.35748655974314403
1114	22	0.5216943137456482
1114	23	2.204805994980326
1114	24	1.0571612927386695
1114	25	0.88526709734181
1115	1	0.15873293752779816
1115	2	0.4906392466381355
1115	3	0.301801350256266
1115	4	0.45630351604127384
1115	5	0.4010747047134231
1115	6	0.0222651720644933
1115	7	-0.08176133673055119
1115	8	-0.05975190131148622
1115	9	-0.13080100796414484
1115	10	0.12383144089611875
1115	11	0.01171155130806767
1115	12	-0.13830530037650135
1115	13	-0.263377689743983
1115	14	0.573614322998985
1115	15	-0.014318542461879247
1115	16	0.3358549234225592
1115	17	0.8201231914630682
1115	18	1.2273956421727377
1115	19	0.48129380576216363
1115	20	0.0631792627533585
1115	21	0.12636121618706397
1115	22	0.508708640611033
1115	23	2.0942486988311444
1115	24	0.7592608423261282
1115	25	0.41420963241235853
1116	1	0.22274510593200603
1116	2	0.19844095331987432
1116	3	0.3145501215456328
1116	4	1.0345812962378176
1116	5	0.244985943462396
1116	6	0.30578958568015935
1116	7	0.09297341128005598
1116	8	0.1538073712951089
1116	9	0.25073315318978473
1116	10	0.7049905198005746
1116	11	0.21339342471493694
1116	12	0.24674361393043398
1116	13	0.15938214671818685
1116	14	0.8172048196009811
1116	15	0.2340723045783335
1116	16	0.5469208355435669
1116	17	0.902285693424318
1116	18	1.3942779783946264
1116	19	0.6020709472674071
1116	20	0.24853476942244868
1116	21	0.24111083687425605
1116	22	0.4958436316118551
1116	23	1.852391807577888
1116	24	0.8549264284886675
1116	25	1.017308065626608
1201	1	1.327185675962646
1201	2	1.5600207840987217
1201	3	1.3784001607929426
1201	4	1.9630223989800655
1201	5	1.635118921719581
1201	6	1.096675445157267
1201	7	1.0992627194561564
1201	8	1.1627063089883052
1201	9	1.420425782486781
1201	10	1.6457063887632586
1201	11	1.2564503031222927
1201	12	1.3711057234294515
1201	13	1.3623010800873558
1201	14	2.328550262141171
1201	15	1.7941943364397446
1201	16	2.099131077435537
1201	17	2.5896427767357437
1201	18	3.0572736095751054
1201	19	2.230275129686561
1201	20	1.976972366201636
1201	21	1.753318283734572
1201	22	2.0939416953463663
1201	23	3.628786718282953
1201	24	2.4638114153705217
1201	25	2.6311813805731106
1202	1	0.9263231263395575
1202	2	1.284693494745941
1202	3	1.0007006276588442
1202	4	1.4610653536167533
1202	5	1.3138707616767604
1202	6	0.9761452401228468
1202	7	0.8384422844085009
1202	8	0.8369790612668737
1202	9	0.9188989897365829
1202	10	1.4993628854847927
1202	11	1.163223947501315
1202	12	1.2501796488201151
1202	13	1.125724404053635
1202	14	1.9863461049530713
1202	15	1.4086368851707292
1202	16	1.871304419987247
1202	17	2.06959932400963
1202	18	2.7063696144519227
1202	19	1.8609929360115487
1202	20	1.567890411906916
1202	21	1.4875940840230157
1202	22	1.913947419657589
1202	23	3.420896647360969
1202	24	2.1742095895382647
1202	25	2.2916860492321334
1203	1	0.9516236057810197
1203	2	1.3423894139699968
1203	3	1.0818291562669913
1203	4	1.7047933976281584
1203	5	1.3914706821380847
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2211	15	-0.11458755605190163
2211	16	-0.1930540420568292
2211	17	0.1353915757538644
2211	18	-4.986255111898068
2211	19	-1.4516314136172284
2211	20	-0.7939427247723216
2211	21	-0.48745488270660076
2211	22	-0.001622749307500726
2211	23	-4.586823045065838
2211	24	-1.7034234879338896
2211	25	-0.9708795009621696
2212	1	-0.002190067705551968
2212	2	0.15932003618099344
2212	3	0.01178916300269961
2212	4	0.5634652002719207
2212	5	0.3504780072201754
2212	6	-0.05886764510280784
2212	7	-0.0634229851163397
2212	8	-0.09196520214547377
2212	9	-0.36076791120691354
2212	10	-3.7389196523722177
2212	11	-1.2480805484840332
2212	12	-0.6467142663716743
2212	13	-0.3245036963872252
2212	14	0.3682961400945999
2212	15	-0.10401278577680984
2212	16	0.07088825914555485
2212	17	0.6303656222227403
2212	18	1.1577716547901318
2212	19	0.3816880992599039
2212	20	-0.03685646262719189
2212	21	-0.061381274314044276
2212	22	0.06557436575262443
2212	23	1.4198002317964722
2212	24	0.597816174846983
2212	25	0.2950038351031097
2213	1	-1.6771559273019534
2213	2	-4.049723715522855
2213	3	-1.8884763523367063
2213	4	-3.664812436971232
2213	5	-1.3699424554662976
2213	6	-1.0555714002302068
2213	7	-1.1213123030056593
2213	8	-0.9323362135509683
2213	9	-0.9760819585406679
2213	10	-1.0158521513736787
2213	11	-1.022500542017326
2213	12	-0.908551064464632
2213	13	-1.2832174558891651
2213	14	-6.365607784942334
2213	15	-2.9620598405726355
2213	16	-2.0435636875231467
2213	17	-0.8421338864344037
2213	18	-0.209843842480538
2213	19	-0.7334249729960263
2213	20	-0.8855364304204585
2213	21	-1.1948643562700312
2213	22	-1.0809357051909458
2213	23	-5.7960168238235426
2213	24	-3.0862231072218584
2213	25	-2.091873570099416
2214	1	-0.03837765832230389
2214	2	0.1799291046717677
2214	3	-0.0159765531949283
2214	4	0.4022693689522967
2214	5	0.018620193422883347
2214	6	-0.10611797658972932
2214	7	-0.3169303026126542
2214	8	-0.29205900984588595
2214	9	-0.16769319963151474
2214	10	0.24180974277258116
2214	11	-0.203688935412913
2214	12	-0.25089236469007403
2214	13	-0.15826839643803936
2214	14	0.4185978861322468
2214	15	-0.018144537886556122
2214	16	0.3274412908567683
2214	17	0.5966798594310632
2214	18	1.171919392624254
2214	19	0.38460270575085076
2214	20	0.19757071558265693
2214	21	0.03762129083707322
2214	22	0.45229060055688564
2214	23	1.929537520667182
2214	24	0.931798231181169
2214	25	0.7663631173564838
2215	1	-2.505468271282261
2215	2	-4.420648589160766
2215	3	-2.4359576883262406
2215	4	-3.9895785897867952
2215	5	-4.728318998741013
2215	6	-2.4550345582846447
2215	7	-1.863577169151121
2215	8	-2.0531753675347417
2215	9	-1.8388818460372676
2215	10	-2.97901825844097
2215	11	-2.1524165262528463
2215	12	-1.8478114829912755
2215	13	-1.8959473788756895
2215	14	-1.1782952273922902
2215	15	-1.7393818862205421
2215	16	-2.5510883443235883
2215	17	-6.995492474680134
2215	18	-3.061580234412503
2215	19	-2.302826265851472
2215	20	-2.131278048392062
2215	21	-2.1274347905168156
2215	22	-1.8535543151671021
2215	23	-6.531059882048719
2215	24	-3.8913326754958995
2215	25	-3.490319996190866
2216	1	-0.8825420067916667
2216	2	-0.3254485103597027
2216	3	-0.6182481186801532
2216	4	-3.1925943451547134
2216	5	-1.190963258778055
2216	6	-0.8898365302489069
2216	7	-0.9281236351334456
2216	8	-0.7906302547345732
2216	9	-0.8142991444849841
2216	10	-0.4558602736166364
2216	11	-1.0366493704705775
2216	12	-1.617865787902943
2216	13	-1.068686087151896
2216	14	-6.592736167773154
2216	15	-2.909713286667184
2216	16	-1.467226252639847
2216	17	-0.5575852887661483
2216	18	0.13653144671447848
2216	19	-0.28244165854074055
2216	20	-0.8479627198094022
2216	21	-0.8795873674568497
2216	22	-0.5248899447116884
2216	23	0.5690455628139546
2216	24	-0.15892654645270454
2216	25	-0.0459675939695785
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87090.0
3	1.0
4	753.0
5	4335.0
6	5042.0
7	4617.0
8	4217.0
9	4055.0
10	3780.0
11	3691.0
12	3610.0
13	3544.0
14	3535.0
15	3717.0
16	3840.0
17	4133.0
18	4676.0
19	5403.0
20	6673.0
21	8219.0
22	10346.0
23	12674.0
24	14842.0
25	17353.0
26	20097.0
27	23262.0
28	25561.0
29	31273.0
30	36907.0
31	41761.0
32	53733.0
33	70526.0
34	91909.0
35	121024.0
36	172246.0
37	241295.0
38	606257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82522727467095	18.418869570423077	12.593739220666672	25.162163934239295
2	13.569097501555403	19.59500265501045	43.07503766012676	23.760862183307392
3	19.390344220979568	24.11899618545002	29.008068889769987	27.482590703800426
4	10.46969535568231	16.354032026987845	39.83908463721983	33.33718798011002
5	12.042814490479877	38.05833129674539	36.79553283691858	13.10332137585615
6	32.089553514011676	36.863986584972004	18.27005832051277	12.776401580503554
7	28.443376398203284	31.091233654164906	21.781612010621732	18.683777937010078
8	24.86571343617529	37.67115484736047	20.28817925077496	17.174952465689284
9	26.2089610053005	15.593379645057375	19.991854149399458	38.205805200242665
10	13.62881284095104	29.8122234223395	35.46523153908205	21.093732197627414
11	34.503157808398946	22.85315735272674	23.887077591863516	18.75660724701079
12	22.77461368766586	25.113249363906814	32.12642234536827	19.98571460305906
13	28.862487384006908	21.833300298227268	25.680580142028177	23.62363217573765
14	20.098722733341535	22.230386460882283	28.83094557832429	28.839945227451892
15	23.38969032703461	30.69730245147796	23.297015141313995	22.615992080173434
16	21.56285208800648	28.652060437526135	28.517519386556607	21.267568087910785
17	21.38148319670707	28.63996345240353	28.599975277966234	21.378578072923162
18	20.876128353162578	27.814389607075647	30.60947019233836	20.700011847423415
19	22.827498752418258	27.539199132275566	28.131601074629316	21.501701040676863
20	22.83769643647474	27.667916196977316	28.701014415072283	20.793372951475657
21	22.543657177230823	27.194251653094053	27.823646422435594	22.43844474723953
22	22.233945100469874	28.201010395635745	27.874466203929465	21.69057829996491
23	20.806000314438897	27.596285519633064	29.69277269029819	21.904941475629848
24	22.0829746591337	27.775483149101554	28.640744895337395	21.500797296427347
25	22.149749551539745	27.35143276571092	28.33784594386286	22.160971738886474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	269.0
3	390.0
4	390.0
5	390.0
6	836.0
7	1282.0
8	1282.0
9	1282.0
10	2205.0
11	3128.0
12	3128.0
13	3128.0
14	5692.0
15	8256.0
16	8256.0
17	8256.0
18	14660.5
19	21065.0
20	21065.0
21	21065.0
22	33386.0
23	45707.0
24	45707.0
25	45707.0
26	64323.5
27	82940.0
28	82940.0
29	82940.0
30	106850.0
31	130760.0
32	130760.0
33	130760.0
34	151260.5
35	171761.0
36	171761.0
37	171761.0
38	188633.5
39	205506.0
40	205506.0
41	205506.0
42	216296.0
43	227086.0
44	227086.0
45	227086.0
46	236959.5
47	246833.0
48	246833.0
49	246833.0
50	233291.0
51	219749.0
52	219749.0
53	219749.0
54	190279.0
55	160809.0
56	160809.0
57	160809.0
58	134844.0
59	108879.0
60	108879.0
61	108879.0
62	86612.0
63	64345.0
64	64345.0
65	64345.0
66	48897.5
67	33450.0
68	33450.0
69	33450.0
70	24155.0
71	14860.0
72	14860.0
73	14860.0
74	10324.0
75	5788.0
76	5788.0
77	5788.0
78	3977.5
79	2167.0
80	2167.0
81	2167.0
82	1452.0
83	737.0
84	737.0
85	737.0
86	486.5
87	236.0
88	236.0
89	236.0
90	158.0
91	80.0
92	80.0
93	80.0
94	50.0
95	20.0
96	20.0
97	20.0
98	17.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11742616872352288
2	0.04698185703050745
3	0.034908943466304326
4	0.030751760965423064
5	0.034738100349829756
6	0.02015948774399956
7	0.01998864462752499
8	0.02408887942291473
9	0.02858774815674514
10	0.034908943466304326
11	0.01987474921654194
12	0.020387278565965658
13	0.01645788688705049
14	0.02124149414833852
15	0.02294992531308425
16	0.02272213449111815
17	0.027221003224948564
18	0.019419167572609745
19	0.03422557100040604
20	0.02300687301857577
21	0.02853080045125362
22	0.0367312700420331
23	0.027847427985355326
24	0.039293916789151694
25	0.031093447198372207
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1755997.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.98600799819198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.66424403473263	72.61549184548694
2	7.766036729408526	12.578806253642632
3	1.4065374333682579	3.417295554855545
4	0.46576996749337596	1.5088340125094464
5	0.20390779153636596	0.82568390181289
6	0.11329706935804872	0.5505286419121587
7	0.0772991610172431	0.4382105330677186
8	0.05361717420551004	0.34737927192383117
9	0.03732560629943897	0.2720566665273357
>10	0.1863600877915048	2.6555136708183817
>50	0.013586125962085461	0.7685335028717231
>100	0.009956801543101022	1.737871736398473
>500	9.243525755765144E-4	0.5821199614780596
>1k	0.0011376647084018637	1.7016744466948401
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4739	0.26987517632433317	No Hit
CCCATGTACTCTGCGTTGATACCAC	3643	0.2074604911056226	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2313	0.13172004280189545	No Hit
GTCCTACAGTGGACATTTCTAAATT	2058	0.11719837790155677	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1966	0.11195918899633654	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1882	0.10717558173504853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.694770549152419E-5	0.0
8	0.0	0.0	0.0	5.694770549152419E-5	0.0
9	0.0	0.0	0.0	1.1389541098304838E-4	0.0
10	1.1389541098304838E-4	0.0	0.0	1.1389541098304838E-4	0.0
11	1.1389541098304838E-4	0.0	0.0	1.7084311647457256E-4	0.0
12	1.1389541098304838E-4	0.0	0.0	1.7084311647457256E-4	0.0
13	1.1389541098304838E-4	0.0	0.0	1.7084311647457256E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	745	0.0	13.263415	4
TGCACCG	75	1.47779265E-5	12.667541	5
TCCAACG	150	1.8189894E-12	12.666458	18
CCGTGTA	60	4.0970024E-4	12.665014	9
TAGGACA	570	0.0	12.1682	4
GTAGGAC	1380	0.0	12.117124	3
CACCGAC	55	0.003072373	12.088987	7
ATCCCGT	95	1.0416552E-6	11.998435	10
CGTTTCC	95	1.0420135E-6	11.998094	14
GGCGAGG	420	0.0	11.987898	19
TGTAGGA	1530	0.0	11.737107	2
CCAACGA	90	7.4633554E-6	11.61092	19
CTGTAGG	1500	0.0	11.542591	1
GTCCTAC	1375	0.0	11.415749	1
TGGCGAG	1100	0.0	11.140726	18
GTAGCAC	180	3.6379788E-12	11.0844145	3
TTGCGAG	60	0.005876895	11.08315	18
TATGGCG	1180	0.0	10.948349	16
ATGGCGA	1205	0.0	10.721204	17
TCCTACA	1545	0.0	10.700683	2
>>END_MODULE