FastQCFastQC Report
Thu 26 May 2016
SRR1513039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755997
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC54440.31002330869585765No Hit
CCCATGTACTCTGCGTTGATACCAC47050.26793895433762127No Hit
GTATCAACGCAGAGTACTTTTTTTT34790.19812106740501267No Hit
TATCAACGCAGAGTACTTTTTTTTT24170.13764260417301397No Hit
GTCCTACAGTGGACATTTCTAAATT21870.1245446319099634No Hit
GGTATCAACGCAGAGTACTTTTTTT21340.12152640351891261No Hit
GAGTACATGGGAAGCAGTGGTATCA21270.12112776958047196No Hit
GTCCTACAGTGTGCATTTCTCATTT20720.11799564577843812No Hit
CTGTAGGACGTGGAATATGGCAAGA18750.10677694779660785No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTC456.8306667E-414.75836858
TAGGACC7550.013.7445954
GCCGGTT802.032144E-613.04385811
GTAGGAC14200.012.6043913
TGTAGGA15650.012.2350932
ACGTGGA5500.012.0750288
GACGTGG5600.012.0291657
AGGACGT9750.012.0102745
GGCGAGG4150.011.88662619
CGTGGAA5600.011.8587279
CTGTAGG15750.011.7383831
CGGGACC657.881625E-411.717344
GGACGTG9950.011.3861336
GTCCTAC14850.011.1663151
CCGTGGA855.3984026E-511.1611569
CTGGTCG600.00593644711.0681469
TAGGACG10750.010.9816014
GCACCGT700.001465189910.8803866
TGGCAAG10900.010.79529818
ATGGCAA11450.010.77677217
Produced by FastQC (version 0.11.5)