FastQCFastQC Report
Thu 26 May 2016
SRR1513038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920436
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC88960.4632281419427672No Hit
CCCATGTACTCTGCGTTGATACCAC76110.3963162531841728No Hit
GAGTACATGGGAAGCAGTGGTATCA36570.1904255075409959No Hit
GTATCAACGCAGAGTACATGGGAAG25650.13356341997338106No Hit
GCGTTGATACCACTGCTTCCCATGT25120.1308036300090188No Hit
CATGTACTCTGCGTTGATACCACTG24160.12580476516790978No Hit
GTATCAACGCAGAGTACTTTTTTTT20770.10815252369774364No Hit
TATCAACGCAGAGTACATGGGAAGC19400.10101872699741099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG250.0060326818.9999495
ATAAGAC1251.4097168E-912.1609163
GCGTTTA2050.011.12076313
CCGATCC600.005883472511.0815719
GTATTGA1900.011.0140281
CGTTTAT2000.010.92383214
TAAGACT2107.2759576E-129.9528724
TAGTACT1356.4388405E-79.8523384
GTCTTTA2550.09.6986261
GTCTTGG3750.09.6389421
ACTGTTC3650.09.6286068
GTTACAC2106.730261E-119.5007163
TAATACC1502.6462294E-79.5004694
AGGACCG800.0045163699.4999735
AGCGTCC800.00452219469.4984898
TGCGTTT2650.09.31975712
GTCTAGA1951.6443664E-99.2682141
CTGCTAC1752.6144335E-89.2403391
GTGTTAC1651.0448093E-79.2238671
ACTATAC1651.0642543E-79.2128163
Produced by FastQC (version 0.11.5)