##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513038_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1920436
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.439292431510346	31.0	28.0	34.0	16.0	34.0
2	28.10104059703109	31.0	28.0	34.0	16.0	34.0
3	28.493454090633584	31.0	28.0	34.0	16.0	34.0
4	31.29444407415816	35.0	32.0	37.0	16.0	37.0
5	31.528608086913597	35.0	32.0	37.0	17.0	37.0
6	31.971378374494126	35.0	32.0	37.0	19.0	37.0
7	31.95545751069028	35.0	32.0	37.0	18.0	37.0
8	31.942640108808625	35.0	32.0	37.0	18.0	37.0
9	33.21278136839759	38.0	33.0	39.0	17.0	39.0
10	32.675510144571334	37.0	32.0	39.0	11.0	39.0
11	33.059170417551016	38.0	33.0	39.0	17.0	39.0
12	32.90833383669125	38.0	33.0	39.0	15.0	39.0
13	32.97275097946508	38.0	33.0	39.0	15.0	39.0
14	33.19011568206386	39.0	32.0	41.0	10.0	41.0
15	33.69460632897946	39.0	33.0	41.0	10.0	41.0
16	33.247203239264415	38.0	32.0	41.0	9.0	41.0
17	32.74935952044223	38.0	32.0	41.0	8.0	41.0
18	32.11334457383636	38.0	30.0	40.0	2.0	41.0
19	33.014249368372596	38.0	32.0	41.0	2.0	41.0
20	33.29465079804795	39.0	32.0	41.0	2.0	41.0
21	33.31167661926771	39.0	33.0	41.0	2.0	41.0
22	32.85900545501126	39.0	32.0	41.0	2.0	41.0
23	31.09797202301977	38.0	28.0	40.0	2.0	41.0
24	32.16046824783539	38.0	31.0	41.0	2.0	41.0
25	31.880419342274358	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1603114399552155
1101	2	1.491592244016097
1101	3	1.209532228081283
1101	4	1.3599900330399137
1101	5	1.538898288212728
1101	6	1.2483569483013
1101	7	1.2273364959750843
1101	8	1.248196101369885
1101	9	1.4604789087659
1101	10	1.7842972797375296
1101	11	1.464172271424033
1101	12	1.5895363191902092
1101	13	1.4466586319765753
1101	14	0.3864523578517094
1101	15	0.9582614764805371
1101	16	1.7095612609243318
1101	17	2.4394603450002137
1101	18	-3.9161084819993803
1101	19	-0.18377674877777395
1101	20	0.8725388754979022
1101	21	1.2981142938011558
1101	22	2.0168954737754348
1101	23	3.8720176192672184
1101	24	2.879006386751634
1101	25	3.1492392247440577
1102	1	1.0817204126238522
1102	2	1.352718022702362
1102	3	1.1522698347001565
1102	4	1.7415600718810964
1102	5	1.5629707485535818
1102	6	1.1298767754434778
1102	7	1.103950154205105
1102	8	1.0117524502342903
1102	9	1.1712870579626795
1102	10	1.4748037052152725
1102	11	1.1538121401609303
1102	12	1.2611485682531907
1102	13	1.265545033676645
1102	14	2.139529039214743
1102	15	1.6140975336635606
1102	16	1.931518027031757
1102	17	1.3849774121186798
1102	18	1.7486587786770897
1102	19	1.669271425298085
1102	20	1.581853631582426
1102	21	1.6793506678038028
1102	22	2.0223669292344226
1102	23	3.6817152800499358
1102	24	2.632560889121301
1102	25	2.9432837932959828
1103	1	0.8562177303831575
1103	2	1.1980523310464335
1103	3	0.9085176504415848
1103	4	1.3449793376472599
1103	5	1.2452257453874935
1103	6	1.0633738152324526
1103	7	0.8499046478089021
1103	8	0.7794556425101717
1103	9	0.8123716497557467
1103	10	1.0891500885444358
1103	11	1.0499445463113446
1103	12	1.0810121436437043
1103	13	0.9100793410979904
1103	14	1.7836328963126178
1103	15	1.2267459911589
1103	16	1.5494972865234047
1103	17	1.9696442917805967
1103	18	2.1063726860749057
1103	19	1.4762760469873868
1103	20	1.390485360108066
1103	21	1.335344157992374
1103	22	1.6454117078327855
1103	23	3.0604165242266888
1103	24	1.965329093006197
1103	25	2.2590283327937186
1104	1	0.9234112452292322
1104	2	1.3705336886895552
1104	3	1.1243097052582094
1104	4	1.8096643532037788
1104	5	1.5199987781617565
1104	6	1.12275696795529
1104	7	1.0751741329416582
1104	8	1.1014258410625004
1104	9	1.29450514453886
1104	10	1.5044440601003544
1104	11	1.187059703458786
1104	12	1.3894675991260854
1104	13	1.1806611268386433
1104	14	2.1013020909730997
1104	15	1.527630512408983
1104	16	1.9373753911746832
1104	17	2.377376370597233
1104	18	3.0572089383417165
1104	19	2.21128922868305
1104	20	1.5613687769226061
1104	21	1.8172704995811273
1104	22	2.0773385930556074
1104	23	3.54710029343056
1104	24	2.4639432839156044
1104	25	2.5666535732087397
1105	1	0.6349629957062959
1105	2	1.0050655428337407
1105	3	0.6679697871627397
1105	4	0.9383874894468889
1105	5	1.0031724134436004
1105	6	0.772444912408865
1105	7	0.44506984045814946
1105	8	0.49355048103277
1105	9	0.6877257310925131
1105	10	1.174993404721171
1105	11	0.7389880736229557
1105	12	0.6170735041497011
1105	13	0.515750759054427
1105	14	1.3651550835974078
1105	15	0.6854396734235735
1105	16	1.1879301764433023
1105	17	1.6594060627762772
1105	18	1.4810377473312002
1105	19	1.1860483973806595
1105	20	0.8985872077647343
1105	21	0.7624727237250966
1105	22	1.064878705549262
1105	23	2.680379190506727
1105	24	1.855782602557106
1105	25	1.8254339881273722
1106	1	-0.7744519390806097
1106	2	-0.30211439334379264
1106	3	-0.5456534187452284
1106	4	-3.1557355554410904
1106	5	-0.8195602467163958
1106	6	-0.7557486811913243
1106	7	-0.7569356817955466
1106	8	-0.6074278068856067
1106	9	-0.6750292019744037
1106	10	-0.5215207412507681
1106	11	-0.5698678198915985
1106	12	-0.5413412311919856
1106	13	-0.7545283819039383
1106	14	-0.023545453074895306
1106	15	-0.5805543688368573
1106	16	-1.685157711194023
1106	17	-6.447353391658723
1106	18	-7.772572279639647
1106	19	-3.720979827776521
1106	20	-2.3015728744652435
1106	21	-1.7025401944694316
1106	22	-2.1487280324053444
1106	23	-5.888524556135639
1106	24	-3.0138876129535106
1106	25	-1.4494455442193512
1107	1	0.7553558579716473
1107	2	1.219055464262162
1107	3	0.867614535880616
1107	4	1.1112602671585918
1107	5	1.3268399368996313
1107	6	0.840397870912156
1107	7	0.6678098580895231
1107	8	0.6597703869987512
1107	9	0.7363237479122873
1107	10	1.2110006409326601
1107	11	0.8631195139736221
1107	12	0.9621980038943292
1107	13	0.9427701028453086
1107	14	1.736861990794786
1107	15	1.1198397517852499
1107	16	-5.5246669885430215
1107	17	-0.9628438445190532
1107	18	0.8968534915572732
1107	19	0.9661856134070206
1107	20	0.857627377746617
1107	21	0.7926157337931699
1107	22	1.0004148533729804
1107	23	2.9990715999185227
1107	24	2.0246116857604193
1107	25	1.577445176531242
1108	1	0.3527891388629385
1108	2	0.5195515473100407
1108	3	0.3489660474681493
1108	4	0.9437154918266941
1108	5	0.7023301853505615
1108	6	0.24113736502523153
1108	7	0.13170574435932636
1108	8	0.2790899744741395
1108	9	0.4173070340361065
1108	10	0.8246121754382258
1108	11	0.4770753225392923
1108	12	0.5199091736116941
1108	13	0.31967619889350374
1108	14	0.8522587149258456
1108	15	0.5744458212548338
1108	16	0.7544478691650625
1108	17	0.9505940532570563
1108	18	0.634994262757445
1108	19	0.628765289042839
1108	20	0.6255899452495086
1108	21	0.6757231416076337
1108	22	0.9689675799578552
1108	23	2.6588263673206214
1108	24	1.5296150989449018
1108	25	1.608072650761585
1109	1	0.38536732290583586
1109	2	0.728416326154683
1109	3	0.5290004467792855
1109	4	0.5051196666162738
1109	5	0.24320054322101825
1109	6	0.300728093098634
1109	7	0.24080067118508808
1109	8	0.15292946951788622
1109	9	0.31633333779106465
1109	10	0.8132741835539719
1109	11	0.45161443409084256
1109	12	0.5565035364783455
1109	13	0.35327609250931147
1109	14	-0.9373121231656611
1109	15	0.06835528233814614
1109	16	-0.3311286462610852
1109	17	0.8794994371574063
1109	18	1.6975325000626569
1109	19	0.8999607155758653
1109	20	0.7090831035697249
1109	21	0.6333930732391124
1109	22	0.43922301612865766
1109	23	-4.1252106494629786
1109	24	-1.5282824351713309
1109	25	-0.23415814324894768
1110	1	0.5909518073581239
1110	2	0.9777165799736842
1110	3	0.6776200475598024
1110	4	1.243506721190009
1110	5	0.9670462774143616
1110	6	0.5181698325104236
1110	7	0.47312711070307856
1110	8	0.5229952809616023
1110	9	0.660877416658785
1110	10	1.0356452613761817
1110	11	0.7231772284787965
1110	12	0.7693770215631162
1110	13	0.7105727155568218
1110	14	1.5166354503090105
1110	15	1.0612018737616467
1110	16	1.4661760720244175
1110	17	1.7167716896796463
1110	18	2.4296528481366906
1110	19	1.6108573554160088
1110	20	1.129486326144459
1110	21	0.8450692348835034
1110	22	1.1980259513627232
1110	23	2.707334221542226
1110	24	1.6892117812285008
1110	25	2.108507259446739
1111	1	0.3417708309107681
1111	2	0.6412007362917542
1111	3	0.42280967923099766
1111	4	1.0092835191005847
1111	5	0.7223243395915802
1111	6	0.18808494834022582
1111	7	0.10397446748100592
1111	8	-0.0718112933706081
1111	9	0.10377511433969033
1111	10	0.40997016463499136
1111	11	0.14995782881475606
1111	12	0.42394643052483616
1111	13	0.2852963942891833
1111	14	-0.6817166898078852
1111	15	-0.04593211977149991
1111	16	0.3355369896346545
1111	17	0.9988201648020691
1111	18	1.6985817537809496
1111	19	0.7101111576592984
1111	20	0.5423880373867718
1111	21	0.3754173054551657
1111	22	0.5221508477328172
1111	23	0.4042394712118629
1111	24	0.39056066876855056
1111	25	-0.19501756734941011
1112	1	0.024932413139289622
1112	2	0.42859050321945347
1112	3	0.1906057962096952
1112	4	0.594441534167018
1112	5	0.4067232670129499
1112	6	-0.1937856021028317
1112	7	-0.18746279900445373
1112	8	-0.20440667459381245
1112	9	-0.14508293868009758
1112	10	0.18844490938685254
1112	11	-0.29973637177705115
1112	12	-0.0929725686186913
1112	13	-0.3393519502918352
1112	14	-0.06886364177063342
1112	15	-0.18239758973717102
1112	16	0.25574001432945437
1112	17	0.32270579732671223
1112	18	0.5076895699269173
1112	19	0.009450155645481573
1112	20	-0.09813255163773249
1112	21	0.056698518380891016
1112	22	-0.07072911979575025
1112	23	1.6854545592243113
1112	24	0.6939559184421
1112	25	0.5873502276143441
1113	1	-0.08238931568153518
1113	2	0.5650205908755233
1113	3	0.2421205838722429
1113	4	0.26950617191928217
1113	5	0.4125751554133785
1113	6	0.04284522737158625
1113	7	-0.060623908129837645
1113	8	-0.24142483359771205
1113	9	-0.08854483451143125
1113	10	0.24165262369335494
1113	11	-0.11825228978023716
1113	12	-0.02338246917813791
1113	13	-0.1793188948852631
1113	14	0.4839631839203449
1113	15	0.05509560655047352
1113	16	0.504223517041595
1113	17	0.9920596901634156
1113	18	1.5870525635364956
1113	19	0.7395478309128904
1113	20	0.4721547171006577
1113	21	0.40937891267614646
1113	22	0.5629035437487957
1113	23	0.19728019652690065
1113	24	0.30601629127559704
1113	25	0.8567402611349308
1114	1	-0.36505246934973457
1114	2	-3.061482357738196
1114	3	-1.0771901692610903
1114	4	0.011441092784185258
1114	5	0.4169532729768832
1114	6	0.273876000949528
1114	7	0.10081603252542592
1114	8	0.17140526810074874
1114	9	0.24391245929280103
1114	10	0.37030377697855954
1114	11	0.037555926400600015
1114	12	0.17084508880105886
1114	13	0.04334643896886803
1114	14	1.0032627046109468
1114	15	0.2228248375981181
1114	16	0.605137947083854
1114	17	1.0598820958835375
1114	18	1.182204808962254
1114	19	0.5750820534942704
1114	20	0.3232934547339994
1114	21	0.31017567508053645
1114	22	0.3732473559501486
1114	23	1.973948716416615
1114	24	0.9792918334310912
1114	25	0.821525480644361
1115	1	-0.379913275331873
1115	2	-0.18680675985516615
1115	3	-0.32157287923862654
1115	4	-0.32713126908673473
1115	5	-0.32181187010940704
1115	6	-0.7308240228007818
1115	7	-0.8614317513413425
1115	8	-0.7950450818261565
1115	9	-0.8276392935735188
1115	10	-0.7045047581912733
1115	11	-0.7755968106285209
1115	12	-0.7593378670963631
1115	13	-0.962583486247361
1115	14	-0.08680522690641368
1115	15	-0.7520341682335427
1115	16	-0.2394465448614298
1115	17	0.2075541378653014
1115	18	0.6442802797675498
1115	19	-0.15204443214584984
1115	20	-0.6216650315609442
1115	21	-0.6204356723310838
1115	22	-0.31412137678519514
1115	23	1.4815123184613341
1115	24	0.38481096591964814
1115	25	-0.00714650666840555
1116	1	-0.08347562566454059
1116	2	-0.07554221750552514
1116	3	0.03662667940240283
1116	4	0.7453200531498183
1116	5	0.07452727860725261
1116	6	-0.10868751355800654
1116	7	-0.2506064254449285
1116	8	-0.19703498781350604
1116	9	-0.03839496012500376
1116	10	0.40635622142347927
1116	11	-0.1551661862269853
1116	12	-0.1721394747549354
1116	13	-0.036087609498260065
1116	14	0.49232114162778373
1116	15	-0.16814327630277148
1116	16	0.3300765028777519
1116	17	0.4820804076238616
1116	18	0.9932551392265196
1116	19	0.325679794857912
1116	20	-0.026891130522258777
1116	21	-0.20741563757832182
1116	22	0.34047133304572696
1116	23	1.620212094925158
1116	24	0.7905420401952981
1116	25	0.8491475832196187
1201	1	1.0626179345997357
1201	2	1.3987955706564144
1201	3	1.0589770491017667
1201	4	1.67179582222672
1201	5	1.5137294157904435
1201	6	0.9723248948444194
1201	7	0.8695219662727176
1201	8	0.9691037057322234
1201	9	1.1360243137597976
1201	10	1.3896871615938124
1201	11	1.0013677101200997
1201	12	1.122518689978591
1201	13	1.0735900462183565
1201	14	2.124989766551778
1201	15	1.4159988574886313
1201	16	1.8496882077493453
1201	17	2.3864343688404404
1201	18	2.964446656675044
1201	19	2.067698832617296
1201	20	1.6889555675923376
1201	21	1.5722656403825326
1201	22	1.9155006566787733
1201	23	3.6362337598457017
1201	24	2.4419864174736006
1201	25	2.5872114611718473
1202	1	1.4438412456803853
1202	2	1.7570782688142828
1202	3	1.543362153055039
1202	4	2.1828823368683814
1202	5	1.8612250293396002
1202	6	1.5378924686356399
1202	7	1.3996340552081428
1202	8	1.4653602872188287
1202	9	1.6646515628341731
1202	10	2.2455345920351633
1202	11	1.8390827874179436
1202	12	1.9333451675589899
1202	13	1.823243004275703
1202	14	2.7881498070164668
1202	15	2.176304210950036
1202	16	2.5101088435837653
1202	17	2.838220340452885
1202	18	3.481128204326417
1202	19	2.61676227195683
1202	20	2.2746113845366764
1202	21	2.196551552253794
1202	22	2.578824351768482
1202	23	4.390557754003375
1202	24	3.0988095480996805
1202	25	3.1984538821885806
1203	1	0.9994952970338105
1203	2	1.2846231896786193
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2211	16	-0.6578102078415782
2211	17	0.00266947665591033
2211	18	-5.705733447629786
2211	19	-2.027212846733047
2211	20	-1.2458159777094338
2211	21	-0.9427914291639183
2211	22	-0.6780792224746577
2211	23	-5.050090338245894
2211	24	-2.485798598247211
2211	25	-1.8505796792496625
2212	1	0.31250644359334245
2212	2	0.6087950751408293
2212	3	0.29952154994060365
2212	4	0.8875185317059362
2212	5	0.7135000628630124
2212	6	0.32572306993130695
2212	7	0.3810780975701924
2212	8	0.1567483785857533
2212	9	-0.07016326012511342
2212	10	-3.760461139141672
2212	11	-1.1257603523431783
2212	12	-0.356227230866331
2212	13	-0.10700768715178555
2212	14	0.8177736438904333
2212	15	0.4340214671568532
2212	16	0.6245188476015358
2212	17	1.117284461218702
2212	18	1.620392489742919
2212	19	0.7956890921966746
2212	20	0.34859653115778855
2212	21	0.17918374581409324
2212	22	0.4430477837502096
2212	23	1.6252722472489864
2212	24	0.9968470714125957
2212	25	0.9703157955174504
2213	1	-1.6731868750481702
2213	2	-3.9474152677228567
2213	3	-1.8274207986816968
2213	4	-3.6227196453186146
2213	5	-1.3429525590938702
2213	6	-1.1204756762504822
2213	7	-0.9423565256311193
2213	8	-0.899497121831569
2213	9	-0.7321890234954438
2213	10	-1.0988357045316413
2213	11	-0.9819285698722027
2213	12	-0.7566575338309747
2213	13	-1.314942523953821
2213	14	-6.585280304791436
2213	15	-3.0844277512089455
2213	16	-2.236847377057142
2213	17	-1.021564720523756
2213	18	-0.07480033616175774
2213	19	-0.6729271086164701
2213	20	-0.7486680624665638
2213	21	-1.0007401864106384
2213	22	-0.8948946942433977
2213	23	-5.700730364394303
2213	24	-3.221463811008011
2213	25	-2.4434620567532725
2214	1	-0.27447698742391324
2214	2	-0.07182025899783895
2214	3	-0.33158092549993157
2214	4	-0.11488404535996821
2214	5	-0.27984826926658357
2214	6	-0.4646161286983457
2214	7	-0.6542545071284387
2214	8	-0.64757996722172
2214	9	-0.5216839122318717
2214	10	-0.023978484695618363
2214	11	-0.628937157045641
2214	12	-0.4461712197365486
2214	13	-0.5252966312919227
2214	14	-0.062455215583206325
2214	15	-0.35445566500512626
2214	16	0.0014277320447604325
2214	17	0.41039673274804755
2214	18	0.9215069599594443
2214	19	-0.12718513735734405
2214	20	-0.11202204135734206
2214	21	-0.29004741674174994
2214	22	0.12811460722247148
2214	23	1.7244782058616792
2214	24	0.7244062942874692
2214	25	0.5644455990204378
2215	1	-2.888451065985489
2215	2	-4.977408421785729
2215	3	-2.974079076591181
2215	4	-4.58911733830579
2215	5	-5.501105212509017
2215	6	-3.082280681577135
2215	7	-2.33543078025291
2215	8	-2.6869656574547633
2215	9	-2.40968042946475
2215	10	-3.928573744146032
2215	11	-2.7014888609265135
2215	12	-2.4745888161027203
2215	13	-2.4483809782468597
2215	14	-1.7636914930086824
2215	15	-2.33086778545432
2215	16	-3.181929991148685
2215	17	-7.626583540313479
2215	18	-4.033011982831908
2215	19	-2.9555203851635703
2215	20	-2.711667995000873
2215	21	-2.527570036370083
2215	22	-2.3100799137134693
2215	23	-7.092931958153571
2215	24	-4.697446495860081
2215	25	-4.512862914352027
2216	1	-1.4002235291811793
2216	2	-0.7684250976407654
2216	3	-1.0622860061814237
2216	4	-3.3560551951199997
2216	5	-1.8613548440366365
2216	6	-1.3828321614178911
2216	7	-1.4299481362052298
2216	8	-1.3496050744364076
2216	9	-1.3560753809341684
2216	10	-1.0778155703952415
2216	11	-1.568372179682811
2216	12	-2.6004938014496872
2216	13	-1.8017649729553078
2216	14	-7.488674601606927
2216	15	-3.983296091002458
2216	16	-2.414829289970463
2216	17	-1.2926871010387373
2216	18	-0.7190411799381344
2216	19	-1.1411585781477598
2216	20	-1.7290802618595613
2216	21	-1.9144040399313411
2216	22	-1.2606339694559843
2216	23	-0.33184299716575083
2216	24	-1.0258164119330537
2216	25	-0.833899859889172
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	121083.0
3	3.0
4	1032.0
5	5944.0
6	7093.0
7	6218.0
8	5766.0
9	5451.0
10	5360.0
11	5068.0
12	4996.0
13	4880.0
14	4959.0
15	5201.0
16	5216.0
17	5625.0
18	6170.0
19	7381.0
20	8954.0
21	11523.0
22	13809.0
23	16732.0
24	19982.0
25	22802.0
26	25955.0
27	29502.0
28	32826.0
29	39187.0
30	45395.0
31	51773.0
32	66494.0
33	85170.0
34	107692.0
35	139303.0
36	189439.0
37	255189.0
38	551263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.40384758752894	17.224056467290918	11.723875823259757	25.648220121920385
2	14.981357697262757	19.337306120832963	39.63561422180824	26.045721960096042
3	19.546010007407105	23.969281864882294	28.427661948412787	28.057046179297814
4	11.541953385069824	15.635279085890252	37.4978969787854	35.32487055025452
5	13.32129734482482	37.128281204585065	35.45206318581434	14.098358264775774
6	34.008337334990244	34.825716991017885	17.372052011470608	13.793893662521262
7	29.374575536750708	30.6486944239591	21.1621230870509	18.81460695223929
8	27.240421334211113	34.44001966670764	19.736864035133404	18.58269496394784
9	27.40163476327809	14.63833479781094	18.63216576185042	39.32786467706055
10	15.174120153453885	28.021533549154988	33.70339020572509	23.100956091666035
11	35.51910319982959	22.323914063594525	22.37474551722306	19.782237219352826
12	23.85798196520328	24.357808533664986	30.234898175375218	21.54931132575651
13	29.5160625042966	20.54257954173793	24.727413437882138	25.213944516083338
14	22.37225249042086	20.27366895878612	27.029041325335655	30.32503722545737
15	25.60181790151749	27.252845809618254	22.509656169531922	24.63568011933234
16	23.967877484864772	26.70846822748049	25.86587459323425	23.457779694420484
17	23.629194814394275	26.515341705166335	26.774621991426773	23.08084148901262
18	23.5663067503578	26.030948774944324	27.667654821996628	22.735089652701248
19	24.421038266167177	26.31147985015231	26.68229663676102	22.585185246919494
20	24.693349388730006	25.891254201130305	26.290208586477533	23.12518782366216
21	24.388921442243838	25.64397046759639	26.528015417268904	23.43909267289087
22	24.363045380672585	25.60497145476518	25.686700420885945	24.345282743676293
23	23.10297788914833	25.337189055568548	27.692082522317367	23.867750532965747
24	23.86802743342456	26.73784785346892	25.65611745989742	23.73800725320909
25	24.141862663649125	25.330319263914248	25.86889318891496	24.658924883521667
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	212.0
1	212.0
2	327.0
3	442.0
4	442.0
5	442.0
6	819.0
7	1196.0
8	1196.0
9	1196.0
10	1788.0
11	2380.0
12	2380.0
13	2380.0
14	4134.5
15	5889.0
16	5889.0
17	5889.0
18	10592.0
19	15295.0
20	15295.0
21	15295.0
22	24171.0
23	33047.0
24	33047.0
25	33047.0
26	46907.0
27	60767.0
28	60767.0
29	60767.0
30	79221.5
31	97676.0
32	97676.0
33	97676.0
34	117236.5
35	136797.0
36	136797.0
37	136797.0
38	158430.5
39	180064.0
40	180064.0
41	180064.0
42	201468.5
43	222873.0
44	222873.0
45	222873.0
46	249538.5
47	276204.0
48	276204.0
49	276204.0
50	271631.0
51	267058.0
52	267058.0
53	267058.0
54	244173.0
55	221288.0
56	221288.0
57	221288.0
58	194184.5
59	167081.0
60	167081.0
61	167081.0
62	139741.0
63	112401.0
64	112401.0
65	112401.0
66	88921.5
67	65442.0
68	65442.0
69	65442.0
70	48636.5
71	31831.0
72	31831.0
73	31831.0
74	22811.5
75	13792.0
76	13792.0
77	13792.0
78	9740.5
79	5689.0
80	5689.0
81	5689.0
82	3876.0
83	2063.0
84	2063.0
85	2063.0
86	1378.5
87	694.0
88	694.0
89	694.0
90	447.0
91	200.0
92	200.0
93	200.0
94	119.5
95	39.0
96	39.0
97	39.0
98	27.5
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11971239864280821
2	0.04566671318388116
3	0.034263052765101255
4	0.030149403572938643
5	0.033221622589870214
6	0.02072446048709772
7	0.01895402918920495
8	0.02291146385508291
9	0.027337542099814836
10	0.03337783711615487
11	0.019006100697966502
12	0.019266458241774264
13	0.016246310733604243
14	0.019735101820628233
15	0.021870033679851866
16	0.021453461609759448
17	0.026348183433345343
18	0.018745743154158744
19	0.032284335432162276
20	0.02171381915356721
21	0.026608540977153105
22	0.035200339922809194
23	0.02702511304724552
24	0.038220487430979215
25	0.028691401327615187
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1920436.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.1260080329648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.17279983750542	59.15749229366585
2	10.881085625662234	15.478563672364597
3	3.0348388885869393	6.475679255051658
4	1.191423781496814	3.3896486981363085
5	0.570640074190642	2.0293675250407617
6	0.3122856810555739	1.3326980315603179
7	0.1901095661107555	0.9465214168435935
8	0.12996317942979316	0.7395009715290491
9	0.08625743615864473	0.5521632387410442
>10	0.39844596925073833	4.828902322922636
>50	0.02039130096655462	0.993192171457038
>100	0.008857640062652768	1.0959040645407474
>500	0.0018596278995469844	0.8602704796254628
>1k	8.926213917825467E-4	1.2467573094940911
>5k	1.4877023196375777E-4	0.8733385490269028
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8896	0.4632281419427672	No Hit
CCCATGTACTCTGCGTTGATACCAC	7611	0.3963162531841728	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3657	0.1904255075409959	No Hit
GTATCAACGCAGAGTACATGGGAAG	2565	0.13356341997338106	No Hit
GCGTTGATACCACTGCTTCCCATGT	2512	0.1308036300090188	No Hit
CATGTACTCTGCGTTGATACCACTG	2416	0.12580476516790978	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2077	0.10815252369774364	No Hit
TATCAACGCAGAGTACATGGGAAGC	1940	0.10101872699741099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.207150876155206E-5	0.0	0.0	0.0
3	0.0	5.207150876155206E-5	0.0	0.0	0.0
4	0.0	5.207150876155206E-5	0.0	0.0	0.0
5	0.0	5.207150876155206E-5	0.0	0.0	0.0
6	0.0	5.207150876155206E-5	0.0	0.0	0.0
7	0.0	5.207150876155206E-5	0.0	0.0	0.0
8	0.0	5.207150876155206E-5	0.0	0.0	0.0
9	0.0	5.207150876155206E-5	0.0	0.0	0.0
10	0.0	5.207150876155206E-5	0.0	0.0	0.0
11	0.0	5.207150876155206E-5	0.0	5.207150876155206E-5	0.0
12	0.0	5.207150876155206E-5	0.0	5.207150876155206E-5	1.0414301752310412E-4
13	0.0	5.207150876155206E-5	0.0	5.207150876155206E-5	1.0414301752310412E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCCG	25	0.00603268	18.999949	5
ATAAGAC	125	1.4097168E-9	12.160916	3
GCGTTTA	205	0.0	11.120763	13
CCGATCC	60	0.0058834725	11.081571	9
GTATTGA	190	0.0	11.014028	1
CGTTTAT	200	0.0	10.923832	14
TAAGACT	210	7.2759576E-12	9.952872	4
TAGTACT	135	6.4388405E-7	9.852338	4
GTCTTTA	255	0.0	9.698626	1
GTCTTGG	375	0.0	9.638942	1
ACTGTTC	365	0.0	9.628606	8
GTTACAC	210	6.730261E-11	9.500716	3
TAATACC	150	2.6462294E-7	9.500469	4
AGGACCG	80	0.004516369	9.499973	5
AGCGTCC	80	0.0045221946	9.498489	8
TGCGTTT	265	0.0	9.319757	12
GTCTAGA	195	1.6443664E-9	9.268214	1
CTGCTAC	175	2.6144335E-8	9.240339	1
GTGTTAC	165	1.0448093E-7	9.223867	1
ACTATAC	165	1.0642543E-7	9.212816	3
>>END_MODULE