FastQCFastQC Report
Thu 26 May 2016
SRR1513038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1920436
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC107260.5585190029764074No Hit
CCCATGTACTCTGCGTTGATACCAC103290.5378466139980713No Hit
GAGTACATGGGAAGCAGTGGTATCA39980.20818189202868514No Hit
GTATCAACGCAGAGTACTTTTTTTT33520.17454369736872252No Hit
CATGTACTCTGCGTTGATACCACTG29870.15553759667075603No Hit
GTATCAACGCAGAGTACATGGGAAG27840.14496708039216094No Hit
GCGTTGATACCACTGCTTCCCATGT26580.1384060702882054No Hit
TATCAACGCAGAGTACATGGGAAGC24310.12658583779933308No Hit
TATCAACGCAGAGTACTTTTTTTTT24060.12528405008029425No Hit
ACGCAGAGTACATGGGAAGCAGTGG23450.12210768804583959No Hit
GGTATCAACGCAGAGTACTTTTTTT20060.10445544657567345No Hit
ACTCTGCGTTGATACCACTGCTTCC19640.10226844320768826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG307.608041E-419.0426434
CGAGCGA500.001520577513.27570310
TGAACCG701.068556E-412.2416995
GGTACGA550.00309426212.07762718
TACACGG657.875394E-411.718555
TTTAGAC1252.1724009E-710.663883
GTATTAT1909.094947E-1210.5356861
TAGGGTG1553.5306584E-910.4427395
TAGGACG1105.886808E-610.3868964
CCGTGCG750.002682144310.117729
TCTACAC3200.010.1164053
TAGTACT1701.4988473E-910.0813994
ATAGTAC1752.4756446E-99.793363
GTCTAGA2106.184564E-119.5322881
GTGTACG800.004391599.5322881
TAATATA1901.0095391E-99.5213214
TTAGGGG1502.5632653E-79.5213214
TTAGGCC1106.671258E-59.5213214
CTAGTAG800.00443349839.5213213
TTAAACC1401.0266995E-69.5213214
Produced by FastQC (version 0.11.5)