FastQCFastQC Report
Thu 26 May 2016
SRR1513037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230346
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC53800.24121817870411139No Hit
CCCATGTACTCTGCGTTGATACCAC43510.1950818393199979No Hit
GTATCAACGCAGAGTACTTTTTTTT23460.10518547346465526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT802.0023163E-613.0614526
TTTGCGC604.0960376E-412.6656517
TAGGACC5700.011.8350374
CGTTTCC1001.9330128E-611.3988314
CCAACGA752.0774301E-411.39831919
GTAGGAC10900.011.2442343
GTCTTAG3950.010.8364971
TGTAGGA12400.010.8044612
TAGGACG7050.010.6469244
GGACGTG6700.010.6334746
AGGACGT7150.010.63042455
CCTATAC2900.010.4837983
TTAGACT2300.010.3275754
TTAGGAC6050.010.20762353
TTACACC2700.010.20517354
ATTACAC2700.010.2047163
GTGTAGG3650.010.1635471
GTGTTAG3850.010.1297011
TAGACTG3500.010.0438545
TGGCGAG8050.010.02957618
Produced by FastQC (version 0.11.5)