##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513037_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2230346
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.190741705547033	31.0	30.0	34.0	23.0	34.0
2	28.87076668821788	31.0	30.0	34.0	16.0	34.0
3	29.254765404112188	31.0	30.0	34.0	22.0	34.0
4	32.125920372892814	35.0	33.0	37.0	19.0	37.0
5	32.36724840002403	35.0	33.0	37.0	22.0	37.0
6	32.81217353720006	37.0	33.0	37.0	26.0	37.0
7	32.80750475486763	37.0	35.0	37.0	26.0	37.0
8	32.79177266666248	37.0	35.0	37.0	26.0	37.0
9	34.166193048074156	39.0	35.0	39.0	25.0	39.0
10	33.67322289904795	39.0	34.0	39.0	19.0	39.0
11	34.04342510085879	39.0	34.0	39.0	25.0	39.0
12	33.874430693712995	39.0	34.0	39.0	23.0	39.0
13	33.95932155817976	39.0	35.0	39.0	24.0	39.0
14	34.26147736718877	39.0	33.0	41.0	16.0	41.0
15	34.80796477317869	40.0	34.0	41.0	22.0	41.0
16	34.40688709285465	39.0	34.0	41.0	17.0	41.0
17	33.94477045265622	39.0	33.0	41.0	11.0	41.0
18	33.359665720027294	39.0	32.0	41.0	10.0	41.0
19	34.25101979692837	39.0	34.0	41.0	18.0	41.0
20	34.503506182448824	39.0	34.0	41.0	18.0	41.0
21	34.52119671118293	40.0	34.0	41.0	18.0	41.0
22	34.10906514056563	39.0	34.0	41.0	10.0	41.0
23	32.44864249762144	39.0	31.0	41.0	2.0	41.0
24	33.538378350264935	39.0	33.0	41.0	2.0	41.0
25	33.28148278338877	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8368765524976922
1101	2	1.2195944465998174
1101	3	0.9315907176752418
1101	4	0.974541359489038
1101	5	1.0757558992480156
1101	6	0.9499108439785999
1101	7	0.7960501163563833
1101	8	0.9164137113550197
1101	9	1.0371395071008962
1101	10	1.3448265184328037
1101	11	1.080910912245109
1101	12	1.0659741136923913
1101	13	0.943311155750834
1101	14	0.07584967525846764
1101	15	0.6740986839211232
1101	16	1.4022798316356955
1101	17	1.9439739729579912
1101	18	-4.190092021574969
1101	19	-0.48346985097638395
1101	20	0.4656603420927894
1101	21	0.9090642833584752
1101	22	1.371352874825277
1101	23	3.0956169038286347
1101	24	2.038856674998833
1101	25	2.3658878810722754
1102	1	0.9848401424970241
1102	2	1.2848112302228358
1102	3	1.1021715653389101
1102	4	1.567336754599495
1102	5	1.3061465166863329
1102	6	1.0000506698684362
1102	7	1.0316023591931582
1102	8	0.9907946381803683
1102	9	1.0099256093188913
1102	10	1.4110864988909597
1102	11	0.977571871998947
1102	12	1.0687524208435235
1102	13	1.036027471132627
1102	14	1.8770798407923266
1102	15	1.3032743052933924
1102	16	1.6172419348268647
1102	17	1.3888211660646803
1102	18	1.7028659854956771
1102	19	1.5527807154885096
1102	20	1.430989913349542
1102	21	1.4927889558787157
1102	22	1.7957959500315113
1102	23	3.2595022434121077
1102	24	2.1022741557800018
1102	25	2.4014323297527227
1103	1	0.8658355459063962
1103	2	1.2085471815161135
1103	3	0.9318215637067091
1103	4	1.4339371456512282
1103	5	1.3422163434599028
1103	6	0.9753686725767068
1103	7	0.8565811520022208
1103	8	0.9230813142305934
1103	9	1.0674579271393583
1103	10	1.210614559454143
1103	11	1.01594570907816
1103	12	1.090389287715034
1103	13	0.899556214202974
1103	14	1.7750951293550585
1103	15	1.2776950085958063
1103	16	1.519234126045184
1103	17	1.9208310170643301
1103	18	2.011142235206613
1103	19	1.4689412396364503
1103	20	1.4144029045222126
1103	21	1.374790380694705
1103	22	1.648054546727657
1103	23	3.1119697802022515
1103	24	1.9543869058897485
1103	25	2.20075730237334
1104	1	0.5351755336989115
1104	2	1.0041635224915773
1104	3	0.726395323775332
1104	4	1.1615700274652454
1104	5	1.0902482133460936
1104	6	0.6378678820640786
1104	7	0.5730982169528858
1104	8	0.7405481409167223
1104	9	0.7489362475656165
1104	10	1.0566707120968033
1104	11	0.7195125199809098
1104	12	0.855015921209727
1104	13	0.6505616656497324
1104	14	1.4866605844278737
1104	15	0.983370450355757
1104	16	1.2285840854938996
1104	17	1.8302636197937971
1104	18	2.409072588043081
1104	19	1.522300707810011
1104	20	1.0338757432248542
1104	21	1.0402468688192812
1104	22	1.3887690017415366
1104	23	2.878722601548546
1104	24	1.7480850556512735
1104	25	1.9124802329366801
1105	1	0.6220285508394134
1105	2	1.0420811689603866
1105	3	0.7361382023318761
1105	4	0.8043118180234785
1105	5	0.8623940636286136
1105	6	0.6169911309579632
1105	7	0.43797572311031274
1105	8	0.42385495922518146
1105	9	0.6476569788787927
1105	10	0.9934376018425937
1105	11	0.5795929426981985
1105	12	0.5786248227354349
1105	13	0.5675122891939566
1105	14	1.2561816910377743
1105	15	0.7089364602299355
1105	16	1.098003740789288
1105	17	1.5703109080172268
1105	18	1.5347644281950892
1105	19	1.112574441775621
1105	20	0.8922451109877869
1105	21	0.7358328485471262
1105	22	1.2415912846712445
1105	23	2.654344369594554
1105	24	1.6192821115083547
1105	25	1.609892197017409
1106	1	-0.6689559024337903
1106	2	-0.23983938923937842
1106	3	-0.38302982272690755
1106	4	-2.7371621533849684
1106	5	-0.6819572023739084
1106	6	-0.7441262909179187
1106	7	-0.7011606660846468
1106	8	-0.6157487973206557
1106	9	-0.543933816618356
1106	10	-0.39821843907388654
1106	11	-0.4619614317977252
1106	12	-0.4471519101374639
1106	13	-0.546758069648746
1106	14	0.1976881786617355
1106	15	-0.31101415488030426
1106	16	-1.3304453209262022
1106	17	-6.15204551656219
1106	18	-7.403499667371342
1106	19	-3.1835664585117733
1106	20	-1.7086332528076085
1106	21	-1.3959045042069533
1106	22	-1.6207375082246003
1106	23	-5.202462346352878
1106	24	-2.3382297377413614
1106	25	-1.0093423868886262
1107	1	0.4715544267912044
1107	2	0.8294374710233612
1107	3	0.509974820255028
1107	4	0.7190734948572839
1107	5	0.7384277528116243
1107	6	0.28472268559346503
1107	7	0.3612630130002543
1107	8	0.34544289025017605
1107	9	0.4277072593925766
1107	10	0.8195364299611398
1107	11	0.39430604921636103
1107	12	0.45295080852530134
1107	13	0.3758878555895606
1107	14	1.1701465944683562
1107	15	0.5619093145334446
1107	16	-5.679161741699932
1107	17	-1.0940211546537952
1107	18	0.5553237111644123
1107	19	0.5924081258658873
1107	20	0.4408470842192287
1107	21	0.45886049457121203
1107	22	0.4704585519604194
1107	23	2.394541613594754
1107	24	1.4387823104006117
1107	25	1.1977383142826383
1108	1	0.4147890064679274
1108	2	0.6458530222283336
1108	3	0.5329009056261427
1108	4	0.882050835977104
1108	5	0.7131192735031036
1108	6	0.2690247662889149
1108	7	0.23318757166820347
1108	8	0.2967281617852393
1108	9	0.25479790648248013
1108	10	0.6994877708504319
1108	11	0.3011308794172294
1108	12	0.37357424159781516
1108	13	0.190729741331495
1108	14	0.8046780234290978
1108	15	0.5212695303543384
1108	16	0.7297763490512779
1108	17	0.8880985503578458
1108	18	0.7586363528065121
1108	19	0.5721764594798842
1108	20	0.5613935608256995
1108	21	0.5226808003660395
1108	22	0.8818032890765295
1108	23	2.542563605318932
1108	24	1.3269351709658679
1108	25	1.4856474508062334
1109	1	0.46350885473739467
1109	2	0.8042966156693225
1109	3	0.5978604758537607
1109	4	0.7455698703199118
1109	5	0.5330678123887438
1109	6	0.35853810348631043
1109	7	0.36740921271734095
1109	8	0.2971496260087605
1109	9	0.41764866285338087
1109	10	0.9092342483060207
1109	11	0.5206253697172016
1109	12	0.5852143652307333
1109	13	0.5018009607671274
1109	14	-0.417333566197307
1109	15	0.10234782537332165
1109	16	0.16081730426783025
1109	17	1.062265889823351
1109	18	1.8016935166107473
1109	19	0.997026471187219
1109	20	0.875175338575616
1109	21	0.8294024339514792
1109	22	0.5994414516767606
1109	23	-4.152605933591019
1109	24	-1.1441734607766776
1109	25	0.08904228072567122
1110	1	0.6895296623273239
1110	2	1.0228751251363875
1110	3	0.8625889777699278
1110	4	1.322740070644322
1110	5	1.0241312424653515
1110	6	0.6557163043901326
1110	7	0.5863391656387122
1110	8	0.5837493378333463
1110	9	0.6539143554201843
1110	10	1.0394263320894837
1110	11	0.5502862107530362
1110	12	0.6094156246889852
1110	13	0.5634585292272831
1110	14	1.3680027988941603
1110	15	0.8300836359157913
1110	16	1.2200222495663766
1110	17	1.4980784628506285
1110	18	2.1113071433764787
1110	19	1.3752892126155132
1110	20	0.9572915197803553
1110	21	0.9050861723299022
1110	22	1.1068394802557364
1110	23	2.6001410594300154
1110	24	1.6938705438898367
1110	25	1.9173553710530555
1111	1	0.5738117202492887
1111	2	0.8629680283207506
1111	3	0.5644402563931621
1111	4	1.0962764741187598
1111	5	0.8761348638427151
1111	6	0.3980977815668254
1111	7	0.2950540383786162
1111	8	0.16390346718718263
1111	9	0.2600492577493938
1111	10	0.6127155022841819
1111	11	0.39249589332044366
1111	12	0.4542553426069702
1111	13	0.4188460908800309
1111	14	-0.191024166716808
1111	15	0.14675895454279697
1111	16	0.5159568720618708
1111	17	1.0287702784173334
1111	18	1.6661503341231025
1111	19	0.8871225650192969
1111	20	0.6233725029321135
1111	21	0.5296767699976854
1111	22	0.8183421711960648
1111	23	0.7537749028475957
1111	24	0.6014163782017832
1111	25	0.16441272090339965
1112	1	0.25729334500750767
1112	2	0.5569678921633532
1112	3	0.3041039476202201
1112	4	0.8325705901168519
1112	5	0.6216109542031703
1112	6	0.10704844853784579
1112	7	0.06950618506017747
1112	8	0.0760068987540734
1112	9	0.09365074106410276
1112	10	0.5539450035876854
1112	11	0.11200620724333277
1112	12	0.22613851955952669
1112	13	0.09316736627438615
1112	14	0.39733405538794386
1112	15	0.2163228904527088
1112	16	0.4792498787776367
1112	17	0.7817741909402116
1112	18	0.8384951647473002
1112	19	0.3620582306487634
1112	20	0.21408784987318086
1112	21	0.3313588745104994
1112	22	0.3755286787988581
1112	23	1.9624704749289208
1112	24	0.9052309478442879
1112	25	0.8924790856210123
1113	1	0.469506283417477
1113	2	0.89147460724244
1113	3	0.5684364780507138
1113	4	0.7369084096995806
1113	5	0.8874158702557509
1113	6	0.3796649349683108
1113	7	0.40811914062415156
1113	8	0.29359475943494573
1113	9	0.4553322626245446
1113	10	0.6968879164810673
1113	11	0.4268787315269762
1113	12	0.4646006628097865
1113	13	0.34316557939862946
1113	14	0.9798484040002577
1113	15	0.4985849322084235
1113	16	0.8402023550300584
1113	17	1.3542784615732657
1113	18	1.9054029405158346
1113	19	1.03659668064126
1113	20	0.6978844605272485
1113	21	0.6666482411702574
1113	22	0.9423256682705841
1113	23	0.6661671634907762
1113	24	0.7845417856031176
1113	25	1.1144662344105853
1114	1	-0.3269069070372659
1114	2	-3.0887656951895295
1114	3	-1.0813414565684631
1114	4	0.11023293391838962
1114	5	0.3564340509098187
1114	6	0.2012236695374625
1114	7	-0.012128803088870654
1114	8	0.10385253598671085
1114	9	0.0263210051984899
1114	10	0.3026340162189314
1114	11	-0.02497925314049354
1114	12	0.003518613731756659
1114	13	-0.009088650868712023
1114	14	0.7419912418026939
1114	15	0.0835848342240979
1114	16	0.45124155279727063
1114	17	0.7910064704136985
1114	18	0.9412185283402295
1114	19	0.3494318188058827
1114	20	0.15467606792337563
1114	21	0.049366170640396945
1114	22	0.2236218976095401
1114	23	1.8134579419096326
1114	24	0.788649572544216
1114	25	0.8014928710367073
1115	1	0.11486342937620364
1115	2	0.3382849390129188
1115	3	0.13688337559483088
1115	4	0.2452641618799234
1115	5	0.27652393893580296
1115	6	-0.08172384176705805
1115	7	-0.2528994289079307
1115	8	-0.26550584504595776
1115	9	-0.25317508448191006
1115	10	-0.05431300118414839
1115	11	-0.0959614823574313
1115	12	-0.15403587190595402
1115	13	-0.3804328151876959
1115	14	0.5229048809483032
1115	15	-0.23169032172669546
1115	16	0.27053956284846237
1115	17	0.7818487933614051
1115	18	1.2251282532769707
1115	19	0.43808043861162105
1115	20	0.01978463573206568
1115	21	0.08085300515746496
1115	22	0.3010866377068169
1115	23	1.9830236684939635
1115	24	0.7436028391017473
1115	25	0.4516327649474903
1116	1	0.3162750712458724
1116	2	0.40504332577168256
1116	3	0.43070308914818156
1116	4	1.0731801152588858
1116	5	0.5195172418362759
1116	6	0.41590700052837093
1116	7	0.2723439398409866
1116	8	0.1788101747393398
1116	9	0.35416802293575245
1116	10	0.7230450070855383
1116	11	0.3374855374552581
1116	12	0.44072654792208255
1116	13	0.3124545046842613
1116	14	1.0169459940456704
1116	15	0.3159126427550447
1116	16	0.7131465323356991
1116	17	0.8668328798465339
1116	18	1.4899105442888825
1116	19	0.7811140113436323
1116	20	0.5561294942618815
1116	21	0.5507959207270758
1116	22	0.7579195084386257
1116	23	2.1775107251750185
1116	24	1.3015252268626938
1116	25	1.5618509219416765
1201	1	0.7183958122224468
1201	2	1.1350570189396265
1201	3	0.8211664004220154
1201	4	1.3805728031690236
1201	5	1.1843020767300558
1201	6	0.714721444193195
1201	7	0.6957676800231738
1201	8	0.6963067514473522
1201	9	0.8544516229716095
1201	10	1.1441546637053932
1201	11	0.812280526312172
1201	12	0.9035652838232195
1201	13	0.8135708997027962
1201	14	1.7632204014220534
1201	15	1.165437013812074
1201	16	1.4314086745900951
1201	17	1.9101634879406504
1201	18	2.4095042157579485
1201	19	1.5015300456414664
1201	20	1.2358350911510882
1201	21	1.1872134881651561
1201	22	1.5346459912727113
1201	23	3.0500943292291893
1201	24	1.8865606498208507
1201	25	2.019813282730375
1202	1	0.9189706507526125
1202	2	1.2322423229646802
1202	3	0.9174807145526245
1202	4	1.582838560908037
1202	5	1.2867910210044613
1202	6	0.9624614585197548
1202	7	0.8273108343534048
1202	8	0.808662967813973
1202	9	1.0303235008761362
1202	10	1.501076998343116
1202	11	1.1323349021298768
1202	12	1.1116001470992671
1202	13	1.0342221663011841
1202	14	1.9035452355121691
1202	15	1.3930843198854745
1202	16	1.6464684376690002
1202	17	2.0097575193288435
1202	18	2.717061329606757
1202	19	1.7860669779810152
1202	20	1.5128764783412478
1202	21	1.3867426948169452
1202	22	1.8615595050102272
1202	23	3.512068997261416
1202	24	2.231729430938202
1202	25	2.370967251839467
1203	1	0.9177042551927208
1203	2	1.2462715885062643
1203	3	0.9739119164115593
1203	4	1.6036730631191247
1203	5	1.3692892594905004
1203	6	1.0445926390292115
1203	7	0.8796052108430388
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2211	16	-0.8198384815445365
2211	17	-0.23058472695795018
2211	18	-5.734083754134929
2211	19	-2.1714904322443758
2211	20	-1.2903155396886419
2211	21	-1.093111741793173
2211	22	-0.6332029504086094
2211	23	-5.050606164221271
2211	24	-2.4743686818802075
2211	25	-1.8468495076247642
2212	1	0.22540770781365183
2212	2	0.46281865496021624
2212	3	0.174152439430177
2212	4	0.7218467646074416
2212	5	0.5797844315100633
2212	6	0.15403774076239785
2212	7	0.02460178927728407
2212	8	-0.05465477627924997
2212	9	-0.4237062071296549
2212	10	-3.7561827781005483
2212	11	-1.2946451248472428
2212	12	-0.5068179596372744
2212	13	-0.27633194273504813
2212	14	0.5790997038748529
2212	15	0.15518118324191477
2212	16	0.3321054681951665
2212	17	0.8657643520596139
2212	18	1.380672339369383
2212	19	0.7194374877823364
2212	20	0.2430409669367819
2212	21	0.22938603843254413
2212	22	0.3578155997397374
2212	23	1.6512333697668637
2212	24	0.8176174371739009
2212	25	0.6544571685862905
2213	1	-1.5360299186058128
2213	2	-4.051044007046372
2213	3	-1.8116740336579866
2213	4	-3.4585498758655646
2213	5	-1.2618315066332855
2213	6	-1.0110713866427083
2213	7	-1.0138424267066384
2213	8	-0.8811471528559203
2213	9	-0.8256427958325574
2213	10	-0.8124885457214788
2213	11	-0.9348660125256814
2213	12	-0.7403830271660041
2213	13	-1.0877784739713476
2213	14	-6.401419160834937
2213	15	-2.900662948387353
2213	16	-2.0085419505652595
2213	17	-0.7636223150185373
2213	18	0.011729543655526697
2213	19	-0.7888133715164116
2213	20	-0.8731378797190601
2213	21	-1.0316314220808636
2213	22	-0.8648884041488074
2213	23	-5.259407918493309
2213	24	-2.8687098273821015
2213	25	-2.1998576973785333
2214	1	-0.08037968155440822
2214	2	0.09980660054151258
2214	3	-0.02310666010568596
2214	4	0.3125832773759143
2214	5	-0.012735346004639325
2214	6	-0.21493551966190694
2214	7	-0.30476617584664467
2214	8	-0.3826721391520209
2214	9	-0.3344964621233615
2214	10	0.15936582478951067
2214	11	-0.36303281111291597
2214	12	-0.34746779897432845
2214	13	-0.3540657965787446
2214	14	0.1641566405680166
2214	15	-0.08512266703149862
2214	16	0.2753381288111356
2214	17	0.4035267212273652
2214	18	0.9054055073420244
2214	19	0.16651666360961315
2214	20	-0.08388722330317933
2214	21	-0.19410340103229373
2214	22	0.2207879581966239
2214	23	1.585647184901127
2214	24	0.610362471655364
2214	25	0.4972510596361417
2215	1	-2.510610082082824
2215	2	-4.395436264444008
2215	3	-2.549949567387795
2215	4	-4.152297372369219
2215	5	-4.919003497687161
2215	6	-2.4438464694086335
2215	7	-1.7834166828987321
2215	8	-2.2090187133455963
2215	9	-1.7722178492776521
2215	10	-3.265295911137823
2215	11	-2.02169202373004
2215	12	-1.7916964873599994
2215	13	-1.8030110616059858
2215	14	-1.1721449138316231
2215	15	-1.7008867650494963
2215	16	-2.520679763225374
2215	17	-6.728050289707465
2215	18	-3.038596271432329
2215	19	-2.3847822182829574
2215	20	-2.1093512907575516
2215	21	-2.0804891140557302
2215	22	-1.8335638103657175
2215	23	-6.360214702250996
2215	24	-3.6442782753139866
2215	25	-3.3597920466716964
2216	1	-1.0273629628506562
2216	2	-0.5653985719765409
2216	3	-0.8229908099597587
2216	4	-3.0551174473240366
2216	5	-1.3665047723872839
2216	6	-1.1783765236116324
2216	7	-1.2835401375715385
2216	8	-1.190579866420407
2216	9	-1.166800829737511
2216	10	-0.8901346510235939
2216	11	-1.480167493272944
2216	12	-2.251011094783671
2216	13	-1.624329546622505
2216	14	-7.376994667997572
2216	15	-3.62090413037161
2216	16	-2.024005280938738
2216	17	-1.1519689996374396
2216	18	-0.3646691905405248
2216	19	-0.8862327086035435
2216	20	-1.332012137768217
2216	21	-1.561075288275724
2216	22	-1.1062391806548675
2216	23	-0.02945152642513449
2216	24	-0.6355402842413795
2216	25	-0.5047088447556618
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111466.0
3	4.0
4	1042.0
5	5548.0
6	6576.0
7	5784.0
8	5381.0
9	5032.0
10	4864.0
11	4725.0
12	4555.0
13	4626.0
14	4644.0
15	4855.0
16	4895.0
17	5257.0
18	5968.0
19	6871.0
20	8638.0
21	10785.0
22	13382.0
23	15971.0
24	19174.0
25	22326.0
26	25666.0
27	29636.0
28	32891.0
29	39649.0
30	47160.0
31	53336.0
32	68330.0
33	90076.0
34	117726.0
35	154918.0
36	218777.0
37	306437.0
38	763375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28973373038709	18.06430008501882	12.136459248871727	25.50950693572236
2	14.065347761355385	19.706245040423493	41.73634184776831	24.492065350452815
3	19.093755186070606	24.191870035388625	29.034280767695435	27.680094010845334
4	10.645332735325447	16.20958681392611	39.379447216460164	33.76563323428828
5	12.471081297100818	37.62539660896531	36.45936400831388	13.444158085619993
6	32.4254098974522	36.491983674509065	18.122398130488225	12.960208297550505
7	28.8217598800306	30.92952066951821	21.823290889016647	18.425428561434536
8	25.598297267771326	36.350561567977444	20.363678274736884	17.68746288951435
9	26.64591958330026	15.064732604359937	19.63196127211105	38.65738654022876
10	13.953497759657694	29.109738649156565	35.20885193108985	21.72791166009589
11	34.921184453044766	22.508868150446883	23.555915314208324	19.014032082300023
12	22.974676476958862	24.79919530132028	31.751560493886956	20.4745677278339
13	28.91746394050211	21.46179699954484	25.50010201816596	24.120637041787088
14	20.711627971637846	21.40408893241902	28.537903279693506	29.346379816249623
15	23.922489471212945	29.507152758562388	23.117722849048743	23.452634921175925
16	22.35313797173619	28.048952215577028	27.51568716791458	22.0822226447722
17	22.05122639364341	27.854884114759106	27.956285662646334	22.137603828951153
18	21.535459006696676	27.33102143446852	29.638645095122545	21.49487446371226
19	23.36735142388897	27.050122936959937	27.63004810732697	21.952477531824123
20	23.404976675477453	27.06250039240448	27.863633387477677	21.668889544640383
21	23.28767246161926	26.558382992502576	27.384862261677473	22.769082284200692
22	22.87459044150462	27.351667500173804	27.285734469295164	22.488007589026413
23	21.500483471378313	26.730181709892058	29.12932993916692	22.640004879562706
24	22.701049664629412	27.222250629520683	27.70828455501163	22.36841515083827
25	22.814550764781654	26.656040214275205	27.581429601696406	22.947979419246735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	178.0
1	178.0
2	312.5
3	447.0
4	447.0
5	447.0
6	871.5
7	1296.0
8	1296.0
9	1296.0
10	2209.5
11	3123.0
12	3123.0
13	3123.0
14	5873.5
15	8624.0
16	8624.0
17	8624.0
18	15899.5
19	23175.0
20	23175.0
21	23175.0
22	37078.0
23	50981.0
24	50981.0
25	50981.0
26	72540.5
27	94100.0
28	94100.0
29	94100.0
30	121408.5
31	148717.0
32	148717.0
33	148717.0
34	175377.5
35	202038.0
36	202038.0
37	202038.0
38	225127.5
39	248217.0
40	248217.0
41	248217.0
42	265708.5
43	283200.0
44	283200.0
45	283200.0
46	298351.0
47	313502.0
48	313502.0
49	313502.0
50	300271.5
51	287041.0
52	287041.0
53	287041.0
54	254495.0
55	221949.0
56	221949.0
57	221949.0
58	188711.5
59	155474.0
60	155474.0
61	155474.0
62	126166.0
63	96858.0
64	96858.0
65	96858.0
66	74366.0
67	51874.0
68	51874.0
69	51874.0
70	37919.0
71	23964.0
72	23964.0
73	23964.0
74	16890.5
75	9817.0
76	9817.0
77	9817.0
78	6805.5
79	3794.0
80	3794.0
81	3794.0
82	2559.0
83	1324.0
84	1324.0
85	1324.0
86	885.5
87	447.0
88	447.0
89	447.0
90	300.0
91	153.0
92	153.0
93	153.0
94	92.5
95	32.0
96	32.0
97	32.0
98	26.5
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11675318538020558
2	0.04810912746273448
3	0.03658625163988009
4	0.032326822833766596
5	0.036765596010663815
6	0.02057976654743255
7	0.01990722515699358
8	0.02322509601649251
9	0.02847091886191649
10	0.034792807932042834
11	0.020445258269344756
12	0.019862389064297648
13	0.01573746853627195
14	0.02120747184517559
15	0.022731898996837262
16	0.022328374162573876
17	0.026767147339471095
18	0.01941402813733833
19	0.03353739733655675
20	0.022597390718749468
21	0.029008951974267668
22	0.03636207117640043
23	0.028605427140004285
24	0.03909707283085225
25	0.030936903960192726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2230346.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.35833815762402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62361547878848	67.01058938644113
2	9.399363473631112	14.542382761191503
3	2.171831684726873	5.040278696656311
4	0.743009200680771	2.299118280019561
5	0.339140510251138	1.3117673137478345
6	0.1847532062928168	0.8575320604862996
7	0.11521223630988106	0.6238838995449107
8	0.0775120500206683	0.47969627014316213
9	0.06282797696849592	0.43742410992794883
>10	0.25896793328143075	3.4763171867760305
>50	0.013990253791825658	0.7562400584855701
>100	0.008021723825409223	1.3442987969934712
>500	8.186600011447424E-4	0.4668338776799051
>1k	8.771357155104892E-4	1.1102684780774927
>5k	5.8475714367365946E-5	0.24336882382891586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5380	0.24121817870411139	No Hit
CCCATGTACTCTGCGTTGATACCAC	4351	0.1950818393199979	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2346	0.10518547346465526	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.4836092695931486E-5	0.0
8	0.0	0.0	0.0	4.4836092695931486E-5	0.0
9	0.0	0.0	0.0	4.4836092695931486E-5	0.0
10	0.0	0.0	0.0	4.4836092695931486E-5	0.0
11	0.0	0.0	0.0	4.4836092695931486E-5	0.0
12	0.0	0.0	0.0	4.4836092695931486E-5	8.967218539186297E-5
13	0.0	0.0	0.0	4.4836092695931486E-5	8.967218539186297E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	80	2.0023163E-6	13.061452	6
TTTGCGC	60	4.0960376E-4	12.66565	17
TAGGACC	570	0.0	11.835037	4
CGTTTCC	100	1.9330128E-6	11.39883	14
CCAACGA	75	2.0774301E-4	11.398319	19
GTAGGAC	1090	0.0	11.244234	3
GTCTTAG	395	0.0	10.836497	1
TGTAGGA	1240	0.0	10.804461	2
TAGGACG	705	0.0	10.646924	4
GGACGTG	670	0.0	10.633474	6
AGGACGT	715	0.0	10.6304245	5
CCTATAC	290	0.0	10.483798	3
TTAGACT	230	0.0	10.327575	4
TTAGGAC	605	0.0	10.2076235	3
TTACACC	270	0.0	10.2051735	4
ATTACAC	270	0.0	10.204716	3
GTGTAGG	365	0.0	10.163547	1
GTGTTAG	385	0.0	10.129701	1
TAGACTG	350	0.0	10.043854	5
TGGCGAG	805	0.0	10.029576	18
>>END_MODULE