FastQCFastQC Report
Thu 26 May 2016
SRR1513037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230346
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC59930.2687027035267174No Hit
CCCATGTACTCTGCGTTGATACCAC58310.26143925650997646No Hit
GTATCAACGCAGAGTACTTTTTTTT33910.15203919033190366No Hit
TATCAACGCAGAGTACTTTTTTTTT24810.11123834597860602No Hit
GAGTACATGGGAAGCAGTGGTATCA23500.10536481783543898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG500.001517827913.27904810
TAGGACC5550.012.0072564
GGACCGT1001.8822975E-611.42404656
CCGGGAC1104.837675E-711.2509563
TACCGTG855.398884E-511.16136557
GACGCTC600.005937456611.0681078
GTCCTAC10400.010.90221
CGGGACA803.6993116E-410.7100454
ACCGTGC1002.4363344E-510.4356448
GACGTGG5000.010.2461347
GTAGGAC10550.010.1065823
GTCCTAA5950.010.0884411
ACCGTGA951.6675203E-49.9862618
GTATAGA3350.09.6701811
AGGACGT8000.09.6390395
TTCTACG800.0044175219.5256132
TCTAGGA4400.09.5256132
CTAGTAC1401.0289059E-69.520043
GACTGCG800.00456740949.4871627
ACCGAGC1106.941872E-59.4869498
Produced by FastQC (version 0.11.5)