FastQCFastQC Report
Thu 26 May 2016
SRR1513036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208884
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81480.3688740558580713No Hit
CCCATGTACTCTGCGTTGATACCAC69980.3168115663837485No Hit
GAGTACATGGGAAGCAGTGGTATCA33220.15039268698582633No Hit
GTATCAACGCAGAGTACTTTTTTTT24180.10946704308601085No Hit
GCGTTGATACCACTGCTTCCCATGT23180.10493987008824365No Hit
GTATCAACGCAGAGTACATGGGAAG22390.10136340342000758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG500.001499195413.3008255
ATACCGT604.0973563E-412.6651566
GTCCTAC7650.011.8112261
GTAGGAC7850.011.255733
CTTACAC3350.010.7770323
TAGGACC4000.010.68888954
GTATAAG3000.010.4622591
TTAGGAC4800.010.2925383
AGGACGT5450.010.2850415
GTCCTAA4350.010.276411
TGCGTTT1856.002665E-1110.26881412
TCCTACA9750.010.2316352
GGACGTG5600.010.1773586
ACCGTCC856.605264E-410.057178
AAAGGCG951.6435048E-410.0006215
GACGTGA3050.09.9655737
TAGGACG5550.09.9292184
AATCCCG1252.5717673E-69.87971719
GTATTAT2900.09.8391151
TAGGACA4850.09.7950884
Produced by FastQC (version 0.11.5)