FastQCFastQC Report
Thu 26 May 2016
SRR1513035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970246
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC117620.5969812906611661No Hit
CCCATGTACTCTGCGTTGATACCAC93590.47501682531013895No Hit
GAGTACATGGGAAGCAGTGGTATCA48740.24738027637158No Hit
GCGTTGATACCACTGCTTCCCATGT34650.17586636389567595No Hit
GTATCAACGCAGAGTACATGGGAAG31160.15815283979766995No Hit
CATGTACTCTGCGTTGATACCACTG31010.1573915135470393No Hit
GCTTCCCATGTACTCTGCGTTGATA28880.1465806807880843No Hit
ACGCAGAGTACATGGGAAGCAGTGG26100.13247076760972995No Hit
TATCAACGCAGAGTACATGGGAAGC23140.1174472629306188No Hit
GTATCAACGCAGAGTACTTTTTTTT21560.1094279597573095No Hit
ACTCTGCGTTGATACCACTGCTTCC20130.1021699828346308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA751.47783885E-512.66768318
CCAACGA1301.9826984E-1012.42375819
TGTAGGA5750.012.3926132
GCGTTTA1850.012.32249613
TAGGACG2550.011.92252354
TGCGTTT1950.011.69027612
TATCGAC1001.9289782E-611.40062419
GTAGGAC4600.011.359033
TCCAACG1850.011.29820318
CGGTTTC855.3323525E-511.17481313
GTGTAAT1303.211244E-810.9744021
CGTTTCC1053.4733093E-610.85553314
GTCCTAG2200.010.8081251
GTGTAAC2200.010.8081251
GTCCTAT2300.010.7517331
CGTTTAT1951.8189894E-1210.71635914
TAGGACT2500.010.6408544
GTTAAAC909.5142816E-510.5558653
CCGGTTT909.5338975E-510.55372112
GTGTAGG3700.010.5393811
Produced by FastQC (version 0.11.5)