##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513035_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970246
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94350705444904	31.0	30.0	34.0	22.0	34.0
2	28.67730679316187	31.0	29.0	34.0	16.0	34.0
3	29.04222213875831	31.0	30.0	34.0	20.0	34.0
4	31.879956107003895	35.0	32.0	37.0	17.0	37.0
5	32.08640545393824	35.0	32.0	37.0	19.0	37.0
6	32.53807849375154	36.0	33.0	37.0	25.0	37.0
7	32.50858826765795	36.0	33.0	37.0	24.0	37.0
8	32.48779187979572	37.0	33.0	37.0	23.0	37.0
9	33.828669110354745	39.0	34.0	39.0	22.0	39.0
10	33.32675615126233	38.0	33.0	39.0	17.0	39.0
11	33.692625692426226	39.0	34.0	39.0	22.0	39.0
12	33.52066645484879	38.0	34.0	39.0	18.0	39.0
13	33.60038238879815	39.0	34.0	39.0	19.0	39.0
14	33.88448092268681	39.0	33.0	41.0	10.0	41.0
15	34.41492382169536	39.0	34.0	41.0	17.0	41.0
16	33.98480798844408	39.0	33.0	41.0	11.0	41.0
17	33.52106945021079	39.0	32.0	41.0	10.0	41.0
18	32.92528800972061	38.0	32.0	41.0	10.0	41.0
19	33.79905301165438	39.0	33.0	41.0	11.0	41.0
20	34.05888046467294	39.0	34.0	41.0	10.0	41.0
21	34.08686377234112	39.0	34.0	41.0	10.0	41.0
22	33.66761967794884	39.0	33.0	41.0	2.0	41.0
23	31.997245521625217	38.0	30.0	41.0	2.0	41.0
24	33.058331294670815	39.0	32.0	41.0	2.0	41.0
25	32.7944896221081	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.43966469171805755
1101	2	0.7906570949653293
1101	3	0.634454840785569
1101	4	0.4708553340500572
1101	5	0.671660886455399
1101	6	0.353982974881454
1101	7	0.3421999820538062
1101	8	0.28959122681666116
1101	9	0.3717006482942864
1101	10	0.7196062277121911
1101	11	0.45532585100175993
1101	12	0.4304531308085586
1101	13	0.40188859995623716
1101	14	-0.4740016352549219
1101	15	0.021818299539837938
1101	16	0.6463991158917679
1101	17	1.1636023707230905
1101	18	-4.904435139024102
1101	19	-1.3163609857306966
1101	20	-0.3022499015032949
1101	21	-0.046361388370399936
1101	22	0.518665026753439
1101	23	2.366685986367358
1101	24	1.4027610232964278
1101	25	1.6276091695656874
1102	1	0.8548873845687801
1102	2	1.1086040850821313
1102	3	0.8962087876473319
1102	4	1.4292207556904089
1102	5	1.273274928298548
1102	6	0.9257773530100621
1102	7	0.7381921525068975
1102	8	0.6744102487650636
1102	9	0.7920542614376558
1102	10	1.1503628699917456
1102	11	0.8064008092869628
1102	12	0.9479348404735575
1102	13	0.8060953770066774
1102	14	1.6778370247373715
1102	15	1.2444533913123976
1102	16	1.5272359789388688
1102	17	1.1952895739062512
1102	18	1.633162730312307
1102	19	1.3537070414801917
1102	20	1.315283149452938
1102	21	1.212186936619979
1102	22	1.6188960625639908
1102	23	3.231260495416258
1102	24	2.1436704059174048
1102	25	2.3854515518016015
1103	1	0.8075287019302166
1103	2	1.0113617663825885
1103	3	0.8421139614409547
1103	4	1.0885537198176678
1103	5	1.0789895661458004
1103	6	0.7542277758897455
1103	7	0.6461790083436583
1103	8	0.7016325278976652
1103	9	0.7712263968359778
1103	10	1.0563457691344667
1103	11	0.8308764512893845
1103	12	0.9373996623903125
1103	13	0.6790081888844455
1103	14	1.6117344644105955
1103	15	1.0549690420221083
1103	16	1.2908955367879145
1103	17	1.6698839807542782
1103	18	1.6997886882740403
1103	19	1.294833610872793
1103	20	1.0596298147537553
1103	21	1.019923938111738
1103	22	1.347769685243911
1103	23	2.9049500806036264
1103	24	1.7117209017846449
1103	25	1.8915450527075421
1104	1	0.6001101933987414
1104	2	1.0496626454555944
1104	3	0.8485698586435682
1104	4	1.3892398191843895
1104	5	1.1746462458662066
1104	6	0.6343847627380157
1104	7	0.7007377740600944
1104	8	0.727211622640425
1104	9	0.8260356977322658
1104	10	1.2502335542222411
1104	11	0.8390612464027853
1104	12	0.8798481525055166
1104	13	0.7704688032837836
1104	14	1.7212850182039077
1104	15	1.176850873536857
1104	16	1.4916529782697694
1104	17	1.9619343672771663
1104	18	2.456376891532159
1104	19	1.6231297585011575
1104	20	1.1101500399983308
1104	21	1.1751792668570928
1104	22	1.536263897003586
1104	23	3.092765864069193
1104	24	2.069716068666942
1104	25	2.084843430418161
1105	1	0.3359061769712639
1105	2	0.6656769494715071
1105	3	0.3904037046539983
1105	4	0.42285734899879657
1105	5	0.7355635789533466
1105	6	0.39945342456900335
1105	7	0.13968016158392516
1105	8	0.16672700440988564
1105	9	0.35327589346907473
1105	10	0.7312112433008195
1105	11	0.4401901369429524
1105	12	0.3299738997565669
1105	13	0.22853221270487012
1105	14	1.068813983320723
1105	15	0.4936720977636
1105	16	0.8996724999618735
1105	17	1.338609064528029
1105	18	1.04022138936444
1105	19	0.7855187254599869
1105	20	0.6173942603642573
1105	21	0.3684335089080619
1105	22	0.825705094281453
1105	23	2.5335747965945856
1105	24	1.3413999344979999
1105	25	1.3089445723058333
1106	1	-0.7026962526596954
1106	2	-0.3333485072755664
1106	3	-0.5733242708590893
1106	4	-2.844510812408526
1106	5	-0.7790994176662522
1106	6	-0.6565692460068995
1106	7	-0.7078400339525928
1106	8	-0.6494243794258345
1106	9	-0.6975390475840655
1106	10	-0.45590397636944147
1106	11	-0.5635857136240929
1106	12	-0.570267157306688
1106	13	-0.48841152009178757
1106	14	0.14661060964303374
1106	15	-0.4825594515605971
1106	16	-1.7002715523755398
1106	17	-6.35402468009724
1106	18	-7.436455947347433
1106	19	-3.387973630788718
1106	20	-1.954250701823419
1106	21	-1.4236002839286215
1106	22	-1.6249124042189464
1106	23	-5.197575718314514
1106	24	-2.496558704594726
1106	25	-1.154879826032552
1107	1	0.6400581824476816
1107	2	0.9202556010344232
1107	3	0.6706626572454866
1107	4	1.0256572548183627
1107	5	0.9206671556297366
1107	6	0.5451394374664886
1107	7	0.4886585654871851
1107	8	0.5020228661390576
1107	9	0.4634039963001513
1107	10	0.8785031198023603
1107	11	0.5911201687288496
1107	12	0.6178747661293542
1107	13	0.4709628963414545
1107	14	1.3912978178596518
1107	15	0.7951342926037341
1107	16	-5.595771085522134
1107	17	-0.8679369116565283
1107	18	0.6883619798234335
1107	19	0.6390945298088582
1107	20	0.6359920619487553
1107	21	0.6042055050536419
1107	22	0.7634629459211055
1107	23	2.5755428624511225
1107	24	1.7384086156667138
1107	25	1.3323377276555917
1108	1	0.21661120118031008
1108	2	0.5783877324639484
1108	3	0.2866542820469924
1108	4	0.8231684170558822
1108	5	0.5126863453628587
1108	6	0.2388804605578585
1108	7	0.16258052406179502
1108	8	0.27604891894223726
1108	9	0.22699603791184586
1108	10	0.7173144525334436
1108	11	0.29398140603120027
1108	12	0.4452644381621269
1108	13	0.1693349752580957
1108	14	0.7349168741441972
1108	15	0.48408134861097807
1108	16	0.8262600625906344
1108	17	0.7847926101599327
1108	18	0.7187414634034113
1108	19	0.5839025999820109
1108	20	0.5575627474541989
1108	21	0.7029828040629553
1108	22	1.0894590984424397
1108	23	2.5890615723326036
1108	24	1.37675916083451
1108	25	1.5454114644975832
1109	1	0.4810921479464483
1109	2	0.7433424969949911
1109	3	0.5675629036828589
1109	4	0.4998616906910769
1109	5	0.40836993072884553
1109	6	0.3219153937506434
1109	7	0.31989932408570354
1109	8	0.25949420440113613
1109	9	0.3254365688920302
1109	10	0.8780070306563204
1109	11	0.47280772899885903
1109	12	0.5253878915981218
1109	13	0.4497039228066839
1109	14	-0.6882371655326125
1109	15	0.0726602756745578
1109	16	-0.2084765392883412
1109	17	0.8851156973652223
1109	18	1.7534214462329487
1109	19	0.9476180485059515
1109	20	0.8492043195293846
1109	21	0.6145151589698514
1109	22	0.5794545884797699
1109	23	-3.8349500122943994
1109	24	-1.11950952426686
1109	25	0.14400707298935345
1110	1	0.8856852524086989
1110	2	1.1118721280637693
1110	3	0.9170484163323778
1110	4	1.2790826608552166
1110	5	1.1448633512978361
1110	6	0.8057950001399874
1110	7	0.7344104088254113
1110	8	0.7506509870436204
1110	9	0.9032387343807358
1110	10	1.1692081312538818
1110	11	0.9913406156520139
1110	12	1.0128545218582516
1110	13	0.9196519384754964
1110	14	1.749659936997297
1110	15	1.2886588474181835
1110	16	1.5979060863929746
1110	17	1.9609468690605425
1110	18	2.650285583345564
1110	19	1.763700635330288
1110	20	1.324575088582428
1110	21	1.1233741291549961
1110	22	1.410291273664761
1110	23	2.7144987437573107
1110	24	1.8755745433232747
1110	25	2.1584532560792127
1111	1	0.6058234629188455
1111	2	0.9064187842564309
1111	3	0.7250726263613352
1111	4	1.1081717990976188
1111	5	0.9262658053460342
1111	6	0.5222025704119773
1111	7	0.3629421250648619
1111	8	0.12161634455606674
1111	9	0.4039735574579524
1111	10	0.6020145756667574
1111	11	0.39955585069394317
1111	12	0.6277289642188464
1111	13	0.39922907460763213
1111	14	-0.3839517691546348
1111	15	0.22327118838768456
1111	16	0.730523534890132
1111	17	1.2328531479570586
1111	18	1.9207903357812057
1111	19	1.058296569599129
1111	20	0.7501449025721811
1111	21	0.6726246675904477
1111	22	0.9476150683085223
1111	23	0.7958438033403041
1111	24	0.8003385158319034
1111	25	0.36000058767139365
1112	1	0.32566957910990624
1112	2	0.6708600687323489
1112	3	0.499238135876233
1112	4	1.0244035933161797
1112	5	0.734458865026717
1112	6	0.18418839873593384
1112	7	0.17272327812450783
1112	8	0.21770566081450227
1112	9	0.13313424772842097
1112	10	0.6285575689752889
1112	11	0.23288155853375514
1112	12	0.2591306588194513
1112	13	0.3471201971362916
1112	14	0.6291733585133343
1112	15	0.27473294488435585
1112	16	0.6539930021778844
1112	17	0.8600370673703068
1112	18	1.036142357242177
1112	19	0.5277708417575866
1112	20	0.34659173975703084
1112	21	0.29195648810684816
1112	22	0.3160777343983483
1112	23	1.9248204897772396
1112	24	0.8981171488346504
1112	25	1.0356991452991693
1113	1	0.41159418114252944
1113	2	0.7754762020153265
1113	3	0.455869085110443
1113	4	0.6140531435950933
1113	5	0.7752209064072133
1113	6	0.25873798140824533
1113	7	0.30858912741554434
1113	8	0.24736766032297908
1113	9	0.2782587473529432
1113	10	0.6348025090328377
1113	11	0.25145328793597344
1113	12	0.3315197040837319
1113	13	0.1789747854444741
1113	14	0.9206065980540004
1113	15	0.4501774747231764
1113	16	0.8626996454639695
1113	17	1.3542934677547223
1113	18	1.8427286250774841
1113	19	0.7940235112818428
1113	20	0.6257542798884543
1113	21	0.5677173788619214
1113	22	0.719709313422868
1113	23	0.43452800617060205
1113	24	0.5302743687282074
1113	25	1.0496304585805163
1114	1	-0.24070266368708104
1114	2	-2.863126945097491
1114	3	-0.8872728048464253
1114	4	0.30270070443218344
1114	5	0.2972697236430406
1114	6	0.2915717129280111
1114	7	0.18891418733766585
1114	8	0.1885727235846275
1114	9	0.3270412288326625
1114	10	0.3220940679011619
1114	11	0.11742237598387817
1114	12	0.140434970464149
1114	13	0.18164553073103207
1114	14	0.8397602229419974
1114	15	0.33471256739010613
1114	16	0.6729206267985575
1114	17	0.9746638830691623
1114	18	1.1640797342556368
1114	19	0.6618233184825897
1114	20	0.5338203946923485
1114	21	0.4182551489726052
1114	22	0.6021289537056731
1114	23	2.3237228023383025
1114	24	1.1738208847020957
1114	25	1.2061681102545947
1115	1	-0.1291707939177762
1115	2	0.12366887327300802
1115	3	-0.04719019962816873
1115	4	0.20498868389986313
1115	5	0.08406733613693973
1115	6	-0.3278246495340156
1115	7	-0.38711726836684335
1115	8	-0.4866746057682718
1115	9	-0.42618163056566516
1115	10	-0.17170714133800402
1115	11	-0.33360088813091693
1115	12	-0.4780291088247779
1115	13	-0.5561275655387945
1115	14	0.4276164626670962
1115	15	-0.4387579188078945
1115	16	-0.01241345314191733
1115	17	0.5323526933103935
1115	18	1.011537319762141
1115	19	0.21590084161025658
1115	20	-0.26643238785140255
1115	21	-0.2743815797549942
1115	22	0.06385718555929998
1115	23	1.9072808889717976
1115	24	0.6512409919151381
1115	25	0.28669271193559354
1116	1	0.19688805443592372
1116	2	0.13733221471737167
1116	3	0.21904607372844964
1116	4	0.9095765029910083
1116	5	0.32191246637730586
1116	6	0.17906448677615572
1116	7	0.05135145329319357
1116	8	0.013205524606782149
1116	9	0.052049237434083295
1116	10	0.5395736229543147
1116	11	-0.00790524025131134
1116	12	0.14086853957561374
1116	13	0.0019912445643726073
1116	14	0.7299411252812433
1116	15	0.09497572819177691
1116	16	0.4148952717505594
1116	17	0.843916744993507
1116	18	1.290195139122659
1116	19	0.5127941082595626
1116	20	0.21720024541937732
1116	21	0.11479064640801084
1116	22	0.5173729122745101
1116	23	1.8303075296592297
1116	24	0.944565555760299
1116	25	1.1009980517457763
1201	1	0.9327957329968832
1201	2	1.270894458103271
1201	3	1.0151650476561542
1201	4	1.6139739412469538
1201	5	1.3691000466087004
1201	6	0.930693474592097
1201	7	0.9979791574069026
1201	8	1.0427898072396076
1201	9	1.207015648910179
1201	10	1.4716278699871452
1201	11	1.1910026020574946
1201	12	1.2712638794332562
1201	13	1.164994238521878
1201	14	2.0050536766546045
1201	15	1.577041194984318
1201	16	1.7897783739837934
1201	17	2.2747276202762734
1201	18	2.8183712913717685
1201	19	1.9586902031767082
1201	20	1.6776918326211714
1201	21	1.5181143233355385
1201	22	1.8705695330041792
1201	23	3.4968809341790745
1201	24	2.320591961616408
1201	25	2.5274750674109896
1202	1	0.9399645715327622
1202	2	1.3013938424945302
1202	3	1.0567392948759498
1202	4	1.5982785846539045
1202	5	1.4634305274164134
1202	6	0.8686099920691177
1202	7	0.7562820814458817
1202	8	0.800194370303906
1202	9	0.8923544329642397
1202	10	1.5495358668062522
1202	11	1.2506852983827699
1202	12	1.2174521156512057
1202	13	1.0760475946004036
1202	14	1.9753682670799364
1202	15	1.4855585126061968
1202	16	1.7787062068241966
1202	17	2.169713734651168
1202	18	2.7388782412165043
1202	19	1.9315311042099168
1202	20	1.5240922648658284
1202	21	1.5633631189282369
1202	22	1.9371268883419859
1202	23	3.617379093799009
1202	24	2.360045068990175
1202	25	2.5196711058324723
1203	1	1.1155770837018757
1203	2	1.5219955763038087
1203	3	1.1147361424954418
1203	4	1.752088097425677
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2211	14	-0.3533984895380371
2211	15	-0.3951950025963029
2211	16	-0.4339834830076654
2211	17	0.07776892906617405
2211	18	-5.446813491639034
2211	19	-1.8656742142067984
2211	20	-1.1275044619261863
2211	21	-0.8179459457602292
2211	22	-0.5834147860634076
2211	23	-5.049289575832045
2211	24	-2.2819090230660635
2211	25	-1.595920879396573
2212	1	0.18960424486880711
2212	2	0.5643751316516656
2212	3	0.3435610769399702
2212	4	0.7657346690693672
2212	5	0.6603057007692144
2212	6	0.29814936381935553
2212	7	0.08910967772843037
2212	8	6.422178182532434E-4
2212	9	-0.14983836116156368
2212	10	-3.7813328222593796
2212	11	-1.0674858096855715
2212	12	-0.2015503718413001
2212	13	-0.030897117596879298
2212	14	0.82916675986489
2212	15	0.3082749379108449
2212	16	0.5435934018674402
2212	17	1.070613878496225
2212	18	1.554030360528266
2212	19	0.8256204946616563
2212	20	0.42210364394946964
2212	21	0.2132673046053526
2212	22	0.472051030530551
2212	23	1.9102276489497783
2212	24	1.1291857718702119
2212	25	1.034858870598839
2213	1	-1.3104381501825486
2213	2	-3.5321961074232
2213	3	-1.563225045936786
2213	4	-3.182952847402028
2213	5	-1.06916301789947
2213	6	-0.8547862998558102
2213	7	-0.7361445448084964
2213	8	-0.5856149982577072
2213	9	-0.5385802941272431
2213	10	-0.682411205613775
2213	11	-0.713082849206522
2213	12	-0.6363274197312023
2213	13	-1.0693022442999904
2213	14	-5.651359745908479
2213	15	-2.397139684672304
2213	16	-1.5947642749897284
2213	17	-0.47323473203653776
2213	18	0.1894004091990027
2213	19	-0.4153208752243671
2213	20	-0.6723588489651533
2213	21	-0.8728932996708849
2213	22	-0.7165341834207624
2213	23	-5.2784269665158945
2213	24	-2.9068706078410678
2213	25	-2.1374427710068495
2214	1	-0.025002458564518548
2214	2	0.11863908990234151
2214	3	0.02335392983295037
2214	4	0.3320586389014082
2214	5	0.06760889132579706
2214	6	-0.05627085927253006
2214	7	-0.13713046670323337
2214	8	-0.16876109807051165
2214	9	-0.06760330858258357
2214	10	0.3076964370579631
2214	11	-0.09791726442622917
2214	12	-0.07535860565073449
2214	13	-0.15936285249640036
2214	14	0.4307197185272287
2214	15	0.1259913056406745
2214	16	0.5204429580275516
2214	17	0.7089600860017171
2214	18	1.2704527840595716
2214	19	0.2523499210919269
2214	20	0.14720977541560387
2214	21	0.028682104962761912
2214	22	0.47883989884131495
2214	23	1.840921562950932
2214	24	0.9553444095921222
2214	25	0.7733761394552445
2215	1	-2.54057111680493
2215	2	-4.320885894253273
2215	3	-2.47432552996991
2215	4	-3.9718147698673008
2215	5	-5.069288233620732
2215	6	-2.5513282542227884
2215	7	-1.9304873081170086
2215	8	-2.2545013574585298
2215	9	-1.8541188263149735
2215	10	-3.2996440168535237
2215	11	-2.30061314805279
2215	12	-1.9697695931921047
2215	13	-1.9296446886682297
2215	14	-1.0861970048106429
2215	15	-1.8168868659752633
2215	16	-2.5466854679568485
2215	17	-7.072347149271824
2215	18	-3.1986703609995395
2215	19	-2.3402289592089005
2215	20	-2.0614884356266217
2215	21	-2.121547350605759
2215	22	-1.855380859312774
2215	23	-6.340982917793642
2215	24	-3.8623108903047054
2215	25	-3.7891279443332344
2216	1	-1.1110926615829761
2216	2	-0.693070208905862
2216	3	-0.9457230577119624
2216	4	-3.1869714657346044
2216	5	-1.3843746932441086
2216	6	-1.1239852627733207
2216	7	-1.088333958140975
2216	8	-1.0561118025965435
2216	9	-1.1511949128078527
2216	10	-0.8968683477294945
2216	11	-1.4328183275329636
2216	12	-2.136278381940425
2216	13	-1.4322204393429345
2216	14	-7.477810424957855
2216	15	-3.6475327502325854
2216	16	-1.9786412599708072
2216	17	-1.1616924954248091
2216	18	-0.33127407603775794
2216	19	-0.7865379054194293
2216	20	-1.3227632867134957
2216	21	-1.5101430161294118
2216	22	-1.0017578186437106
2216	23	-0.06309916510330282
2216	24	-0.7470383209016802
2216	25	-0.6388629588540269
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107121.0
3	7.0
4	898.0
5	5458.0
6	6496.0
7	5838.0
8	5400.0
9	5063.0
10	4840.0
11	4682.0
12	4655.0
13	4608.0
14	4619.0
15	4524.0
16	4731.0
17	5114.0
18	5599.0
19	6680.0
20	8110.0
21	10251.0
22	12537.0
23	15363.0
24	18038.0
25	21003.0
26	23902.0
27	27647.0
28	30470.0
29	35980.0
30	43042.0
31	48757.0
32	62487.0
33	81159.0
34	105323.0
35	138078.0
36	191842.0
37	264535.0
38	645389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.887464584341096	17.055104123053408	11.825883003329174	25.231548289276322
2	14.151266273367181	18.630371264831247	41.349256241488185	25.869106220313387
3	20.393669284494294	22.976260832302653	27.875447051440617	28.754622831762433
4	11.029178068977421	15.30607271416459	37.95712900392459	35.70762021293339
5	13.017449547209434	37.25870294136164	35.79091616215568	13.93293134927324
6	34.596037154154615	34.57821851138693	17.248141606936983	13.577602727521478
7	30.00538613402996	30.36444481446266	20.25150859979298	19.3786604517144
8	26.683088069928374	36.10481742442203	19.17923105391598	18.032863451733615
9	26.58436966129274	15.446553908092369	18.884497346035808	39.08457908457908
10	14.614444153349051	29.146890796579157	33.780943270923835	22.457721779147956
11	35.75708441188251	22.5915842451188	21.75081084730688	19.900520495691808
12	23.465477819788575	24.698151750577704	30.52180252037704	21.31456790925668
13	29.49534196173667	20.493134774468917	24.682171125036042	25.32935213875837
14	21.218411566091845	21.00438261837207	27.475001611816673	30.302204203719413
15	25.424327680369093	28.138684612112158	22.205255094115103	24.231732613403647
16	23.00902162275494	27.61476538755005	26.792762598466968	22.583450391228045
17	23.16160138740743	27.44384091724852	27.075970812050663	22.31858688329339
18	22.782193482490914	26.74429643076701	28.379333994293006	22.094176092449068
19	23.87356101246234	26.850743343619616	27.127294992826005	22.14840065109204
20	23.885204647576074	26.621217067104748	27.122232144262288	22.37134614105689
21	23.450379447105867	26.22876081124715	27.27642879046031	23.044430951186673
22	23.306983788658002	26.554634136682193	26.340676307800727	23.797705766859078
23	21.86331998111397	26.219189626899393	28.352600128952993	23.564890263033643
24	22.993136210526103	27.441448595710717	26.70789950169234	22.857515692070844
25	23.46753152465236	25.984325125948835	26.465426073376523	24.082717276022276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	281.5
3	415.0
4	415.0
5	415.0
6	762.0
7	1109.0
8	1109.0
9	1109.0
10	1728.0
11	2347.0
12	2347.0
13	2347.0
14	4331.5
15	6316.0
16	6316.0
17	6316.0
18	11425.0
19	16534.0
20	16534.0
21	16534.0
22	26108.5
23	35683.0
24	35683.0
25	35683.0
26	51075.0
27	66467.0
28	66467.0
29	66467.0
30	87296.0
31	108125.0
32	108125.0
33	108125.0
34	128987.5
35	149850.0
36	149850.0
37	149850.0
38	171919.5
39	193989.0
40	193989.0
41	193989.0
42	215618.5
43	237248.0
44	237248.0
45	237248.0
46	267001.0
47	296754.0
48	296754.0
49	296754.0
50	286480.0
51	276206.0
52	276206.0
53	276206.0
54	246859.0
55	217512.0
56	217512.0
57	217512.0
58	187644.0
59	157776.0
60	157776.0
61	157776.0
62	129747.0
63	101718.0
64	101718.0
65	101718.0
66	79529.0
67	57340.0
68	57340.0
69	57340.0
70	42105.5
71	26871.0
72	26871.0
73	26871.0
74	19058.0
75	11245.0
76	11245.0
77	11245.0
78	7798.0
79	4351.0
80	4351.0
81	4351.0
82	2977.0
83	1603.0
84	1603.0
85	1603.0
86	1035.5
87	468.0
88	468.0
89	468.0
90	304.0
91	140.0
92	140.0
93	140.0
94	80.0
95	20.0
96	20.0
97	20.0
98	15.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11115363259207227
2	0.04689769703884693
3	0.034208926195003056
4	0.03126513135923128
5	0.03461496686200607
6	0.020251278266774808
7	0.018931646099015046
8	0.023296583269297337
9	0.027966050939831875
10	0.034208926195003056
11	0.019946747766522555
12	0.019388441849393426
13	0.0161401165133694
14	0.021266379934282317
15	0.021520155351159198
16	0.02146940026778382
17	0.026849439105573615
18	0.019439196932768802
19	0.03207721269323729
20	0.02258601210204208
21	0.027306234855952
22	0.035477803279387446
23	0.027204724689201244
24	0.039588965032792856
25	0.030300784775099152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1970246.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.89912075012323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.02741633065236	62.834513064898786
2	10.192786448068702	15.064759130120972
3	2.5779352461126948	5.71521444115442
4	0.9654482896090469	2.853831189272756
5	0.4359748333652516	1.6109078427436794
6	0.21448954676647525	0.9510353349680972
7	0.13513710316229616	0.6990559173088668
8	0.08874474395582135	0.5246526839624008
9	0.06264289084579953	0.4166329099275476
>10	0.27280939199421533	3.4415843342305417
>50	0.014634051103125283	0.7428797625257055
>100	0.008917507443626375	1.2814413353766996
>500	0.0018103190894514756	0.9820039133957178
>1k	0.0011140425165855096	1.7947234214955372
>5k	6.962765728659435E-5	0.48156010612892014
>10k+	6.962765728659435E-5	0.6052046124894068
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11762	0.5969812906611661	No Hit
CCCATGTACTCTGCGTTGATACCAC	9359	0.47501682531013895	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4874	0.24738027637158	No Hit
GCGTTGATACCACTGCTTCCCATGT	3465	0.17586636389567595	No Hit
GTATCAACGCAGAGTACATGGGAAG	3116	0.15815283979766995	No Hit
CATGTACTCTGCGTTGATACCACTG	3101	0.1573915135470393	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2888	0.1465806807880843	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2610	0.13247076760972995	No Hit
TATCAACGCAGAGTACATGGGAAGC	2314	0.1174472629306188	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2156	0.1094279597573095	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2013	0.1021699828346308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.075508337537546E-5
7	0.0	0.0	0.0	0.0	5.075508337537546E-5
8	0.0	0.0	0.0	0.0	1.0151016675075093E-4
9	0.0	0.0	0.0	0.0	1.0151016675075093E-4
10	0.0	0.0	0.0	0.0	1.0151016675075093E-4
11	0.0	0.0	0.0	5.075508337537546E-5	1.0151016675075093E-4
12	0.0	0.0	0.0	1.0151016675075093E-4	1.0151016675075093E-4
13	0.0	0.0	0.0	1.0151016675075093E-4	1.0151016675075093E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	75	1.47783885E-5	12.667683	18
CCAACGA	130	1.9826984E-10	12.423758	19
TGTAGGA	575	0.0	12.392613	2
GCGTTTA	185	0.0	12.322496	13
TAGGACG	255	0.0	11.9225235	4
TGCGTTT	195	0.0	11.690276	12
TATCGAC	100	1.9289782E-6	11.400624	19
GTAGGAC	460	0.0	11.35903	3
TCCAACG	185	0.0	11.298203	18
CGGTTTC	85	5.3323525E-5	11.174813	13
GTGTAAT	130	3.211244E-8	10.974402	1
CGTTTCC	105	3.4733093E-6	10.855533	14
GTCCTAG	220	0.0	10.808125	1
GTGTAAC	220	0.0	10.808125	1
GTCCTAT	230	0.0	10.751733	1
CGTTTAT	195	1.8189894E-12	10.716359	14
TAGGACT	250	0.0	10.640854	4
GTTAAAC	90	9.5142816E-5	10.555865	3
CCGGTTT	90	9.5338975E-5	10.553721	12
GTGTAGG	370	0.0	10.539381	1
>>END_MODULE