FastQCFastQC Report
Thu 26 May 2016
SRR1513035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970246
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC139950.7103173918383796No Hit
CCCATGTACTCTGCGTTGATACCAC123120.6248965865176227No Hit
GAGTACATGGGAAGCAGTGGTATCA53800.27306234855951994No Hit
CATGTACTCTGCGTTGATACCACTG37380.1897225016571535No Hit
GTATCAACGCAGAGTACATGGGAAG35460.17997752564908137No Hit
GCGTTGATACCACTGCTTCCCATGT33770.17139991655864292No Hit
ACGCAGAGTACATGGGAAGCAGTGG32440.164649490469718No Hit
GCTTCCCATGTACTCTGCGTTGATA32340.16414193963596424No Hit
GTATCAACGCAGAGTACTTTTTTTT31130.15800057454754382No Hit
TATCAACGCAGAGTACATGGGAAGC29090.14764653753896723No Hit
ACTCTGCGTTGATACCACTGCTTCC26620.13511003194524945No Hit
TATCAACGCAGAGTACTTTTTTTTT21790.11059532667494314No Hit
GGTATCAACGCAGAGTACTTTTTTT20560.10435245141977194No Hit
GGTATCAACGCAGAGTACATGGGAA20320.10313432941876294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG801.9545841E-613.0893485
GGACCGA701.0704674E-412.239396
TAGGACC3300.011.8272894
GTAGGAC5150.011.4604013
GTCCTAC6000.011.1171571
TGGCGAG3300.010.92902718
AGGACCG700.001466371810.8794575
TAGGACT2550.010.8261284
CTAGAAC2600.010.6179333
TAGGACG2900.010.5043044
GTCTTAG2550.010.4632081
AGGACTG4650.010.440775
ACACCGA1105.9003287E-610.3849376
TCTACAC3600.010.312823
TCTAGAC2500.010.2810873
CAGGACT5200.010.2517984
CGTCCAC951.6708007E-49.98418310
TAGACAG3350.09.9457735
GTCCACG1159.900647E-69.9432971
CCATCCA4200.09.9386599
Produced by FastQC (version 0.11.5)