FastQCFastQC Report
Thu 26 May 2016
SRR1513034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459710
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70270.4813969898130451No Hit
CCCATGTACTCTGCGTTGATACCAC53190.36438744682162894No Hit
GAGTACATGGGAAGCAGTGGTATCA31090.21298751121798165No Hit
GTATCAACGCAGAGTACTTTTTTTT26100.17880263888032555No Hit
GTATCAACGCAGAGTACATGGGAAG18790.12872419864219606No Hit
GCGTTGATACCACTGCTTCCCATGT18710.1281761445766625No Hit
CATGTACTCTGCGTTGATACCACTG17370.11899623897897527No Hit
GCTTCCCATGTACTCTGCGTTGATA16400.11235108343438081No Hit
GGTATCAACGCAGAGTACTTTTTTT15070.10323968459488529No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC456.765401E-414.7767918
GGACCGT400.00528123614.2490496
TAGGACC3400.013.692144
GCCGACG500.001501775113.29729112
GTAGGAC5900.013.0415023
TGTAGGA7500.012.5413122
GCGTTTA1201.00353645E-811.87298713
ACCGTGC658.0274E-411.6915278
ATCGAAC658.0274E-411.6915278
TGGCGAG4300.011.48839318
TAGGACT2400.011.4799744
CGTTTAT1251.8355422E-811.398066514
GTCCTAC6800.011.3293991
GTGTAGG3450.011.3030471
GTCCGGA600.005824512311.0962851
CAAGGCG600.005875286611.0833545
CCTAGGT1201.2759301E-711.0825943
ACACCGT600.00587828511.0825946
TATGGCG5350.011.00898816
CAGGACT3300.010.9401644
Produced by FastQC (version 0.11.5)