FastQCFastQC Report
Thu 26 May 2016
SRR1513034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459710
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80460.5512053764103829No Hit
CCCATGTACTCTGCGTTGATACCAC72220.4947558076604257No Hit
GTATCAACGCAGAGTACTTTTTTTT40520.27758938419275064No Hit
GAGTACATGGGAAGCAGTGGTATCA34150.23395057922464052No Hit
TATCAACGCAGAGTACTTTTTTTTT29340.2009988285344349No Hit
GGTATCAACGCAGAGTACTTTTTTT26040.17839159833117538No Hit
GTATCAACGCAGAGTACATGGGAAG21490.14722102335395387No Hit
CATGTACTCTGCGTTGATACCACTG21380.14646744901384523No Hit
GCGTTGATACCACTGCTTCCCATGT19650.13461577984668185No Hit
GCTTCCCATGTACTCTGCGTTGATA19240.13180700276082236No Hit
ACGCAGAGTACATGGGAAGCAGTGG19150.13119044193709709No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16950.11611895513492405No Hit
TATCAACGCAGAGTACATGGGAAGC16810.11515986052024031No Hit
ACTCTGCGTTGATACCACTGCTTCC14890.10200656294743475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA400.005202275214.2814366
ACCGTCC604.1379826E-412.6498098
GTAGGAC6400.012.1987273
TAGGACA2550.011.9478684
GGACGTG4950.011.5405546
GTCTAAC1251.7542334E-811.4330111
CCTATAC1751.8189894E-1211.4251493
CACCGTC752.0994538E-411.3848297
TGTAGGA7550.011.3545612
TAGGACG5000.011.234734
ACTGTTC2800.011.1815288
AAGGCGT1201.2364944E-711.1077836
GTCCTAC6800.011.0687241
ACTGCGC600.005933891511.06858358
GGCGAGA3000.011.06744619
ATAGGAC1900.011.0242663
AGGACGT5150.010.9075045
GCACCGT1053.374822E-610.8810946
AACACCG803.694362E-410.7110775
GACGTGG3550.010.689987
Produced by FastQC (version 0.11.5)