##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513034_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1459710 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.429224983044577 31.0 31.0 34.0 26.0 34.0 2 30.5756780456392 31.0 31.0 34.0 27.0 34.0 3 30.577460591487352 31.0 31.0 34.0 27.0 34.0 4 33.78888683368614 37.0 35.0 37.0 28.0 37.0 5 33.73370052955724 37.0 35.0 37.0 28.0 37.0 6 33.68620273890019 37.0 35.0 37.0 29.0 37.0 7 33.59911968815724 37.0 35.0 37.0 28.0 37.0 8 33.59519014050736 37.0 35.0 37.0 28.0 37.0 9 35.130950668283425 39.0 35.0 39.0 28.0 39.0 10 35.04348260955943 39.0 35.0 39.0 28.0 39.0 11 35.04374773071363 39.0 35.0 39.0 27.0 39.0 12 34.95445670715416 39.0 35.0 39.0 27.0 39.0 13 34.92602366223428 39.0 35.0 39.0 27.0 39.0 14 36.09517849435847 40.0 36.0 41.0 27.0 41.0 15 36.077833268251915 40.0 36.0 41.0 27.0 41.0 16 36.03529879222585 40.0 36.0 41.0 27.0 41.0 17 35.95297285077173 40.0 36.0 41.0 27.0 41.0 18 35.87149022751095 40.0 36.0 41.0 27.0 41.0 19 35.947536154441636 40.0 36.0 41.0 27.0 41.0 20 35.85121496735653 40.0 36.0 41.0 27.0 41.0 21 35.87558898685355 40.0 36.0 41.0 27.0 41.0 22 35.84886107514507 40.0 36.0 41.0 27.0 41.0 23 35.78616643031835 40.0 36.0 41.0 27.0 41.0 24 35.65805194182406 40.0 36.0 41.0 27.0 41.0 25 35.633495694350245 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5617812611559216 1101 2 0.4120874301038633 1101 3 0.5682048694530302 1101 4 0.7534562827001352 1101 5 0.6227697340307969 1101 6 0.4955125918849177 1101 7 0.34215797487213706 1101 8 0.35312839116580363 1101 9 0.47597303365583343 1101 10 0.440799386511479 1101 11 0.38996247879912005 1101 12 0.37894168121660243 1101 13 0.42940371035530234 1101 14 0.42904434307212114 1101 15 0.3492251163469007 1101 16 0.24886250066848703 1101 17 0.4411662107508576 1101 18 0.3183913570797543 1101 19 0.40746179411004135 1101 20 0.488519837893854 1101 21 0.5888492934041878 1101 22 0.660746052454634 1101 23 0.5691637717130504 1101 24 0.6591459865124278 1101 25 0.7703390252968063 1102 1 0.7496393503563006 1102 2 0.6371957806768727 1102 3 0.6274861724847192 1102 4 0.9436070702186328 1102 5 0.6985321489651355 1102 6 0.6253821847657619 1102 7 0.5968486257986783 1102 8 0.6383722516864339 1102 9 0.725014441920564 1102 10 0.7783629858971324 1102 11 0.7669303089531851 1102 12 0.6755126229947024 1102 13 0.6552153663284415 1102 14 0.8469903396510432 1102 15 0.7153042718239533 1102 16 0.7200931556641521 1102 17 0.824041613050241 1102 18 0.6742206092130019 1102 19 0.7112904998290972 1102 20 0.7622024396446534 1102 21 0.7700520654321892 1102 22 0.8460415209072352 1102 23 0.8090971818303316 1102 24 0.9255368183468917 1102 25 0.8710181594042012 1103 1 0.15632318026145597 1103 2 0.34512340124804197 1103 3 0.5926777567456902 1103 4 -0.48557669264262415 1103 5 0.13958559713981344 1103 6 0.26430142154577396 1103 7 0.34776013134816 1103 8 0.5012330790431392 1103 9 0.5684669346127791 1103 10 0.5338686504265553 1103 11 0.6060220331707242 1103 12 0.6548143248215226 1103 13 0.6438041058292683 1103 14 0.7379058328551196 1103 15 0.6725984732844665 1103 16 0.6225133821675968 1103 17 0.6419659261223245 1103 18 0.7513811426259309 1103 19 0.6661870559491163 1103 20 0.68329507254996 1103 21 0.6087051865103632 1103 22 0.7886468896273087 1103 23 0.7861396013805688 1103 24 0.7765979192427181 1103 25 0.649579929767377 1104 1 0.5434738900914304 1104 2 0.5421249560570729 1104 3 0.563168225532312 1104 4 0.1985291156988751 1104 5 0.42244594705432803 1104 6 0.4120345575453399 1104 7 0.28652413297317025 1104 8 0.4727171218228321 1104 9 0.3324319239807707 1104 10 0.32018368396830965 1104 11 0.37170717149042076 1104 12 0.45890512597591027 1104 13 0.4373557338904064 1104 14 0.37224183195898775 1104 15 0.3186074323296495 1104 16 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13 -0.07824625378653138 2211 14 0.09775753657351771 2211 15 0.05460749651808783 2211 16 0.07614398717460347 2211 17 0.1638471336164855 2211 18 0.05883992017346884 2211 19 0.02037846323579373 2211 20 0.0451358550996801 2211 21 0.04720860574223451 2211 22 0.013774691963270413 2211 23 0.00314086398990554 2211 24 -3.0927823299014747 2211 25 -2.1711121195467697 2212 1 -0.034160512112716646 2212 2 -0.34618341241929684 2212 3 -0.5504541512792152 2212 4 0.1954501071377237 2212 5 -0.027869877790998032 2212 6 -0.141452560298589 2212 7 -0.15453368811859747 2212 8 -0.2714856361831153 2212 9 -0.5041769442885808 2212 10 -0.5040616633341344 2212 11 -0.36522058251539136 2212 12 -0.37183026408555264 2212 13 -0.45061393410874473 2212 14 -0.5047889167426121 2212 15 -0.43860020345901063 2212 16 -0.4944105474126772 2212 17 -0.464435906584832 2212 18 -0.546628492942304 2212 19 -0.5100503364589883 2212 20 -0.7483570919694031 2212 21 -0.6272953912010166 2212 22 -0.4954074543825939 2212 23 -0.46171404037340835 2212 24 -0.3320317814628666 2212 25 -0.4744779133886823 2213 1 -0.3036697057818536 2213 2 -0.8180371286644075 2213 3 -0.4377456203346135 2213 4 0.02903359591724808 2213 5 -0.26462033644134664 2213 6 -0.3215412246914653 2213 7 -0.5376665030737229 2213 8 -0.46457507996925784 2213 9 -0.5903743796907222 2213 10 -0.6287426742965749 2213 11 -0.5424642212344182 2213 12 -0.6486726738426754 2213 13 -0.6914354972583396 2213 14 -0.8569910631013329 2213 15 -0.814466497631436 2213 16 -0.7433230006947298 2213 17 -0.8900176885795972 2213 18 -0.8347124252359919 2213 19 -0.9533784514132506 2213 20 -6.311401550060438 2213 21 -3.5246799549858494 2213 22 -2.1203860955474383 2213 23 -1.5758933277331053 2213 24 -1.1827721089618066 2213 25 -1.091321841569183 2214 1 0.08675548221449247 2214 2 -0.15061425987531507 2214 3 0.04921762374438288 2214 4 0.5047634434117967 2214 5 0.12217845475971956 2214 6 -0.053182239520332075 2214 7 -0.02185794309058764 2214 8 0.006791846729740314 2214 9 -0.17772134715153953 2214 10 -0.2844654938903588 2214 11 -0.1781069227594756 2214 12 -0.28449528962733694 2214 13 -0.127353692493422 2214 14 -0.06386153889020818 2214 15 -0.09862420372462566 2214 16 -0.11018223184238707 2214 17 0.010630283871030599 2214 18 -0.11008244421132929 2214 19 -0.09879696290386875 2214 20 -0.1254208065085365 2214 21 -0.13809200080776662 2214 22 -0.11065948088723587 2214 23 -0.14678938508077977 2214 24 -0.0591906907314268 2214 25 -0.14231347836727082 2215 1 -0.14844336008551906 2215 2 0.05842770674922093 2215 3 -0.05593066077138076 2215 4 0.372713471804758 2215 5 0.03139444274308545 2215 6 -0.11724627938038168 2215 7 -0.2918614235177657 2215 8 -0.37469447885506213 2215 9 -0.34593698709218756 2215 10 -0.4136439298962955 2215 11 -0.5454606990229607 2215 12 -0.42472664644439817 2215 13 -0.5568673485905862 2215 14 -0.5133314148770936 2215 15 -0.5948374989564442 2215 16 -0.6520002970912344 2215 17 -0.48979982365585073 2215 18 -0.5714879872495544 2215 19 -0.6769473906300476 2215 20 -0.5392908583485863 2215 21 -0.4955161183362904 2215 22 -0.6064655661393843 2215 23 -0.5839663922797698 2215 24 -0.5910725027517287 2215 25 -0.73102150716322 2216 1 -1.5446591915183667 2216 2 -1.0423736332207199 2216 3 -2.7412143146589933 2216 4 -1.1539510833561124 2216 5 -1.226538991513948 2216 6 -1.0872725660596316 2216 7 -1.1703570697130203 2216 8 -1.2525982239816784 2216 9 -1.3255336476726 2216 10 -1.3079300070504019 2216 11 -1.3461756449561264 2216 12 -1.4527057315613376 2216 13 -1.3552152782304034 2216 14 -1.50152534048258 2216 15 -1.5681506005102008 2216 16 -1.698015117353222 2216 17 -1.605078240590963 2216 18 -1.7987758416748179 2216 19 -1.6489765372498226 2216 20 -1.5094907954737948 2216 21 -1.5179867996340235 2216 22 -1.9155533642752474 2216 23 -1.8622153607647576 2216 24 -1.8087556843728478 2216 25 -1.8490795071961514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40867.0 3 0.0 4 665.0 5 5078.0 6 6046.0 7 4708.0 8 3845.0 9 3319.0 10 2833.0 11 2744.0 12 2344.0 13 2263.0 14 2140.0 15 2200.0 16 2232.0 17 2447.0 18 2628.0 19 3056.0 20 4056.0 21 5347.0 22 6689.0 23 8332.0 24 9498.0 25 10589.0 26 11677.0 27 12602.0 28 13943.0 29 16771.0 30 21203.0 31 26759.0 32 33860.0 33 44015.0 34 58925.0 35 80301.0 36 117935.0 37 197473.0 38 690320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.17165000672621 18.28697285084023 13.206491748730945 27.334885393702624 2 13.942228595871631 19.25569515432031 39.32576721433742 27.476309035470635 3 18.163277749195217 22.980059724232706 28.447245307112585 30.409417219459495 4 10.494892821176808 16.11477622267437 36.17821348075987 37.21211747538894 5 13.079281202764351 37.727947333151526 35.1491464522645 14.04362501181962 6 34.4399059289516 35.47702548445215 16.663159631967652 13.419908954628598 7 29.623694845422975 30.45119761484093 20.2089069413911 19.716200598344994 8 26.368613326261297 36.936575778694674 18.591431737397286 18.10337915764674 9 25.981726116522218 15.279244483947476 18.81498669513809 39.924042704392214 10 14.611210829919298 29.53776906950934 33.18670101256457 22.664319088006796 11 35.73358581360488 22.453023049329566 21.716272097732883 20.09711903933266 12 23.37063686403692 25.144393028574402 30.1169258090463 21.368044298342376 13 29.387007331576203 20.661328590406672 24.870630326457597 25.081033751559527 14 21.239356378157634 21.30423374486273 26.871561311166786 30.584848565812848 15 24.786806676403774 28.77013758916167 22.296856631600292 24.146199102834267 16 22.860024418886677 28.012450617529254 26.434129984666257 22.693394978917812 17 22.915370425696953 27.949467844544273 26.553639022357928 22.58152270740084 18 22.64230877595635 27.216234206479946 27.88413617988589 22.257320837677817 19 23.60906480637579 26.846779554160644 27.502518382478915 22.041637256984657 20 23.5530345068541 26.66659815990847 27.566365921998205 22.214001411239217 21 23.215844243034574 26.298168814353534 27.420515033808083 23.065471908803804 22 23.052729651780147 26.84012577840804 26.59822841523316 23.50891615457865 23 22.299117357718117 26.771450180447047 27.122479290378624 23.806953171456207 24 22.663956522602916 27.186937598864425 27.443496990137024 22.705608888395638 25 22.874406560207163 26.24452802268944 26.84505826499784 24.036007152105554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 69.0 1 69.0 2 211.0 3 353.0 4 353.0 5 353.0 6 830.0 7 1307.0 8 1307.0 9 1307.0 10 1910.5 11 2514.0 12 2514.0 13 2514.0 14 3852.5 15 5191.0 16 5191.0 17 5191.0 18 9139.5 19 13088.0 20 13088.0 21 13088.0 22 20725.5 23 28363.0 24 28363.0 25 28363.0 26 40190.0 27 52017.0 28 52017.0 29 52017.0 30 66548.0 31 81079.0 32 81079.0 33 81079.0 34 96735.5 35 112392.0 36 112392.0 37 112392.0 38 127063.5 39 141735.0 40 141735.0 41 141735.0 42 155861.0 43 169987.0 44 169987.0 45 169987.0 46 193619.0 47 217251.0 48 217251.0 49 217251.0 50 211505.5 51 205760.0 52 205760.0 53 205760.0 54 184038.5 55 162317.0 56 162317.0 57 162317.0 58 139646.5 59 116976.0 60 116976.0 61 116976.0 62 96331.5 63 75687.0 64 75687.0 65 75687.0 66 58753.0 67 41819.0 68 41819.0 69 41819.0 70 30513.0 71 19207.0 72 19207.0 73 19207.0 74 13487.5 75 7768.0 76 7768.0 77 7768.0 78 5435.0 79 3102.0 80 3102.0 81 3102.0 82 2168.5 83 1235.0 84 1235.0 85 1235.0 86 804.0 87 373.0 88 373.0 89 373.0 90 241.0 91 109.0 92 109.0 93 109.0 94 59.5 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18647539579779546 2 0.06343725808551014 3 6.850675819169561E-5 4 0.0 5 0.018633838228141207 6 0.3761021024724089 7 0.0011646148892588254 8 0.030211480362537763 9 0.03212966959190524 10 0.0034253379095847804 11 0.003973391975118345 12 0.010413027245137733 13 0.009111398839495516 14 0.002260723020325955 15 1.3701351638339122E-4 16 0.012605243507271994 17 0.024799446465393813 18 0.06597200813860286 19 0.03014297360434607 20 0.0 21 0.0 22 0.0 23 1.3701351638339122E-4 24 6.165608237252605E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1459710.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.90907294294094 #Duplication Level Percentage of deduplicated Percentage of total 1 84.87867354804641 61.88425401014587 2 10.545035342433302 15.376575019347193 3 2.4272993644437393 5.309164392497482 4 0.8620525709241418 2.5140581509663207 5 0.4055256726926566 1.4783250425292045 6 0.22102905675275997 0.9669014172777844 7 0.14338264380223065 0.7317726945009986 8 0.09847440726658771 0.5743742193930013 9 0.07044521611585335 0.4622485860244791 >10 0.3155485842050838 3.98803169307484 >50 0.018342791852401157 0.9465301740480209 >100 0.010337813470072435 1.4282015055200021 >500 0.0023493829235537948 1.2165110323663009 >1k 0.0013156544371901251 2.0769416459656256 >5k 1.879506338843036E-4 1.0461104163429278 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 8046 0.5512053764103829 No Hit CCCATGTACTCTGCGTTGATACCAC 7222 0.4947558076604257 No Hit GTATCAACGCAGAGTACTTTTTTTT 4052 0.27758938419275064 No Hit GAGTACATGGGAAGCAGTGGTATCA 3415 0.23395057922464052 No Hit TATCAACGCAGAGTACTTTTTTTTT 2934 0.2009988285344349 No Hit GGTATCAACGCAGAGTACTTTTTTT 2604 0.17839159833117538 No Hit GTATCAACGCAGAGTACATGGGAAG 2149 0.14722102335395387 No Hit CATGTACTCTGCGTTGATACCACTG 2138 0.14646744901384523 No Hit GCGTTGATACCACTGCTTCCCATGT 1965 0.13461577984668185 No Hit GCTTCCCATGTACTCTGCGTTGATA 1924 0.13180700276082236 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1915 0.13119044193709709 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1695 0.11611895513492405 No Hit TATCAACGCAGAGTACATGGGAAGC 1681 0.11515986052024031 No Hit ACTCTGCGTTGATACCACTGCTTCC 1489 0.10200656294743475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 6.850675819169561E-5 0.0 13 0.0 0.0 0.0 6.850675819169561E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGA 40 0.0052022752 14.281436 6 ACCGTCC 60 4.1379826E-4 12.649809 8 GTAGGAC 640 0.0 12.198727 3 TAGGACA 255 0.0 11.947868 4 GGACGTG 495 0.0 11.540554 6 GTCTAAC 125 1.7542334E-8 11.433011 1 CCTATAC 175 1.8189894E-12 11.425149 3 CACCGTC 75 2.0994538E-4 11.384829 7 TGTAGGA 755 0.0 11.354561 2 TAGGACG 500 0.0 11.23473 4 ACTGTTC 280 0.0 11.181528 8 AAGGCGT 120 1.2364944E-7 11.107783 6 GTCCTAC 680 0.0 11.068724 1 ACTGCGC 60 0.0059338915 11.0685835 8 GGCGAGA 300 0.0 11.067446 19 ATAGGAC 190 0.0 11.024266 3 AGGACGT 515 0.0 10.907504 5 GCACCGT 105 3.374822E-6 10.881094 6 AACACCG 80 3.694362E-4 10.711077 5 GACGTGG 355 0.0 10.68998 7 >>END_MODULE