FastQCFastQC Report
Thu 26 May 2016
SRR1513033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221957
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC87500.39379699967191084No Hit
CCCATGTACTCTGCGTTGATACCAC68370.30770172420078334No Hit
GAGTACATGGGAAGCAGTGGTATCA37020.1666098848897616No Hit
GTATCAACGCAGAGTACATGGGAAG24670.11102825122178332No Hit
GCGTTGATACCACTGCTTCCCATGT23870.10742782151049728No Hit
GTATCAACGCAGAGTACTTTTTTTT22610.10175714471522176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1354.7384674E-911.2583396
GTCCTAC9000.010.9909851
TAGGACC4150.010.759614
ACCGTGC1355.7010766E-810.5542178
TACACCG1201.5237292E-610.2919845
CTACACG750.002647164710.1338744
TTAGGAC4650.010.0112873
GTAGGAC8550.09.8894163
GTTAGAC1451.6164631E-79.8281113
TAGGACG6050.09.7360564
TATGGCG5900.09.66022816
TGGCGAG5150.09.59166418
ACTGTTC5650.09.5828568
AGGACGT5950.09.5801275
GTATTAA2700.09.5114291
GTCCTAA3800.09.5114291
GTCTTAC2500.09.5114291
GGACGTG5900.09.4992246
GGACGGT900.00111474329.4992236
TCCTACA10900.09.4135582
Produced by FastQC (version 0.11.5)