##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513033_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2221957
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.97620340987697	31.0	30.0	34.0	23.0	34.0
2	28.713485904542708	31.0	29.0	34.0	16.0	34.0
3	29.073411411651982	31.0	30.0	34.0	22.0	34.0
4	31.921070029708044	35.0	32.0	37.0	17.0	37.0
5	32.12058604194411	35.0	32.0	37.0	19.0	37.0
6	32.576594416543614	36.0	33.0	37.0	25.0	37.0
7	32.552507991828826	36.0	33.0	37.0	25.0	37.0
8	32.533292948513406	36.0	33.0	37.0	25.0	37.0
9	33.86883769577899	39.0	34.0	39.0	23.0	39.0
10	33.36689908940632	38.0	33.0	39.0	17.0	39.0
11	33.730280108931	38.0	34.0	39.0	22.0	39.0
12	33.56846059577211	38.0	34.0	39.0	19.0	39.0
13	33.64713988614541	38.0	34.0	39.0	22.0	39.0
14	33.92555796534317	39.0	33.0	41.0	11.0	41.0
15	34.456922883746174	39.0	34.0	41.0	17.0	41.0
16	34.03510103930904	39.0	33.0	41.0	15.0	41.0
17	33.56386014670851	39.0	32.0	41.0	10.0	41.0
18	32.95862611202647	38.0	32.0	41.0	10.0	41.0
19	33.84969781143379	39.0	33.0	41.0	16.0	41.0
20	34.10765149820631	39.0	34.0	41.0	13.0	41.0
21	34.13441934294858	39.0	34.0	41.0	10.0	41.0
22	33.71397151250002	39.0	33.0	41.0	2.0	41.0
23	32.01753769312367	38.0	30.0	41.0	2.0	41.0
24	33.08584819598219	39.0	32.0	41.0	2.0	41.0
25	32.824519106355346	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6718603252278079
1101	2	1.1589604171252432
1101	3	0.8716540476567793
1101	4	0.904584072528209
1101	5	1.1311832287788022
1101	6	0.6595712156644993
1101	7	0.7105629048782944
1101	8	0.7062907582737026
1101	9	0.8033146084791483
1101	10	1.181062710276791
1101	11	0.8396379860641616
1101	12	0.8645269821397932
1101	13	0.7860203578200284
1101	14	-0.27040000006305576
1101	15	0.4278285314312029
1101	16	1.0517938964318319
1101	17	1.7823168105353488
1101	18	-4.59933753372043
1101	19	-0.8596910961844131
1101	20	0.14678539496735965
1101	21	0.5830441858840061
1101	22	1.2328074313954858
1101	23	3.009960698521809
1101	24	2.076439553401805
1101	25	2.2031372344346565
1102	1	0.8897043199772412
1102	2	1.210651328854322
1102	3	1.0111016246690205
1102	4	1.5775775700598373
1102	5	1.489315532245321
1102	6	1.0510925300607
1102	7	0.9055345430481552
1102	8	0.8639081283340033
1102	9	1.0774589450552838
1102	10	1.2831590561392687
1102	11	0.9743639341861368
1102	12	1.00778054466236
1102	13	1.001219160582984
1102	14	1.9777299075796222
1102	15	1.4481334759392013
1102	16	1.670763759667409
1102	17	1.343242610858617
1102	18	1.579240595444844
1102	19	1.4535233907887175
1102	20	1.3795128548204332
1102	21	1.4498276067830176
1102	22	1.8181514008369248
1102	23	3.3670936734938834
1102	24	2.2074033832105755
1102	25	2.5500312681584063
1103	1	0.7557538434432018
1103	2	1.0476020725400943
1103	3	0.8044566080698772
1103	4	1.2037479754328189
1103	5	1.086896788062674
1103	6	0.7932510310161547
1103	7	0.7341178220719797
1103	8	0.6978635395179609
1103	9	0.7771181138900758
1103	10	1.114824789760064
1103	11	0.8195995296877783
1103	12	0.9764158246867254
1103	13	0.784296969802611
1103	14	1.625101610935694
1103	15	1.107317369922498
1103	16	1.3402453321531667
1103	17	1.772721018362006
1103	18	1.681554520856757
1103	19	1.156275053680659
1103	20	1.1033571683170464
1103	21	1.0859182750196936
1103	22	1.40616615714179
1103	23	2.867813451740922
1103	24	1.9160046481825646
1103	25	1.9736670501557825
1104	1	0.5228543980330187
1104	2	0.9399492487733099
1104	3	0.7490135271203933
1104	4	1.3235144503602427
1104	5	1.2019162663915566
1104	6	0.8027084956317765
1104	7	0.6805706174753965
1104	8	0.6497226509279912
1104	9	0.8607423508187537
1104	10	1.2311507020613845
1104	11	0.7951368973522506
1104	12	1.0039288084486984
1104	13	0.8182694960118724
1104	14	1.699144615819776
1104	15	1.1337859800529486
1104	16	1.4030636626250939
1104	17	1.8320565849525323
1104	18	2.5245707502906285
1104	19	1.6611872024422354
1104	20	0.9705102810893678
1104	21	1.1111414753961526
1104	22	1.4111905131106681
1104	23	2.866322398020273
1104	24	1.8426213065049524
1104	25	1.9383347152703436
1105	1	0.5684282484820748
1105	2	0.8430645570581525
1105	3	0.5574299723422307
1105	4	0.7324985231511221
1105	5	0.8399994511606366
1105	6	0.5379472355826636
1105	7	0.26623251326738284
1105	8	0.3354109519135591
1105	9	0.4638155285240231
1105	10	0.9698406176320375
1105	11	0.519115672285821
1105	12	0.506877864925535
1105	13	0.5026529263324306
1105	14	1.2354311542166343
1105	15	0.5765623936558271
1105	16	0.9925912283017055
1105	17	1.4636239938798425
1105	18	1.396414152355952
1105	19	1.041698319533289
1105	20	0.8294306114065719
1105	21	0.6859451183037564
1105	22	1.1186788241873913
1105	23	2.5422804692638437
1105	24	1.5940273410167123
1105	25	1.496235418287597
1106	1	-0.4866897435243871
1106	2	-0.08886617655619489
1106	3	-0.3393024517082637
1106	4	-2.390194599350899
1106	5	-0.5103947655384253
1106	6	-0.5053401604030938
1106	7	-0.4772863894779391
1106	8	-0.32756243572922017
1106	9	-0.37189247811541293
1106	10	-0.29447976806695664
1106	11	-0.23554312824708745
1106	12	-0.3094769410237532
1106	13	-0.3553703663052161
1106	14	0.38940908463356294
1106	15	-0.28300882743312883
1106	16	-1.115875694624016
1106	17	-5.863436634682849
1106	18	-6.905530176820449
1106	19	-2.9910866412634434
1106	20	-1.6245751653422928
1106	21	-1.295906990373382
1106	22	-1.4682877274538981
1106	23	-5.113279515011172
1106	24	-2.2527591376781153
1106	25	-0.7587796514920342
1107	1	0.6742707270053927
1107	2	0.9409059118674996
1107	3	0.639734596919002
1107	4	1.0610895199601487
1107	5	1.0098285652282826
1107	6	0.5449983905924682
1107	7	0.4793253658059484
1107	8	0.530540896355113
1107	9	0.5708702201633002
1107	10	0.9624449748930033
1107	11	0.6807147826566862
1107	12	0.6892637652513045
1107	13	0.5227017785293455
1107	14	1.4942572968482324
1107	15	0.8221479460573349
1107	16	-5.918571475338091
1107	17	-1.0690256856214475
1107	18	0.7396666470143103
1107	19	0.6589244075806846
1107	20	0.6188506008367156
1107	21	0.6204618378864026
1107	22	0.6686674135526829
1107	23	2.6270912629235283
1107	24	1.6986064754366552
1107	25	1.3055446945330615
1108	1	0.1265541995814523
1108	2	0.3416402605356126
1108	3	0.18585695479629294
1108	4	0.6494285625662926
1108	5	0.36615367578947655
1108	6	0.0415751923926706
1108	7	0.005325789566121841
1108	8	0.12125328316231077
1108	9	0.16813956908992367
1108	10	0.6369837301938119
1108	11	0.28970579752174075
1108	12	0.2149054797430594
1108	13	0.07892975203994013
1108	14	0.7825811496459778
1108	15	0.5083978070849469
1108	16	0.6827163297971168
1108	17	0.8862498750751016
1108	18	0.6224326688829009
1108	19	0.5711676128025971
1108	20	0.3896338576465297
1108	21	0.37549855194054516
1108	22	0.8007992313520305
1108	23	2.4994362989989014
1108	24	1.3458838332277807
1108	25	1.5467478692875005
1109	1	-0.003212059883598073
1109	2	0.359729956498807
1109	3	0.2041025949711397
1109	4	0.2881260810192714
1109	5	0.11787517265301517
1109	6	-0.06079201228013176
1109	7	-0.1449058248373305
1109	8	-0.21307788107046122
1109	9	0.037256923177871215
1109	10	0.46182858118882564
1109	11	0.0710748587928336
1109	12	0.228192964529498
1109	13	0.08221989867605117
1109	14	-1.1885429712321312
1109	15	-0.3903497842759265
1109	16	-0.5421337946736529
1109	17	0.4233024876219531
1109	18	1.2945601731560927
1109	19	0.49483338158328394
1109	20	0.2681035117232682
1109	21	0.1932996297385401
1109	22	-0.07702362673790475
1109	23	-4.6538242157258125
1109	24	-1.8645009798316927
1109	25	-0.6502432361167294
1110	1	0.668045165892913
1110	2	0.9889832717828746
1110	3	0.6724249053208489
1110	4	1.158741652112404
1110	5	0.8805122915580128
1110	6	0.49453564491912516
1110	7	0.410188332258393
1110	8	0.4189722793022881
1110	9	0.5479804952302132
1110	10	0.7919840596839762
1110	11	0.4418337382215043
1110	12	0.527362005345779
1110	13	0.2611304934261014
1110	14	1.1987315595783414
1110	15	0.6396537104504745
1110	16	1.1308252892005939
1110	17	1.4780674693176081
1110	18	2.056717851610159
1110	19	1.261343181139857
1110	20	0.795781744815784
1110	21	0.6789776353055501
1110	22	0.8891210680300787
1110	23	2.259217497557664
1110	24	1.4698222258882012
1110	25	1.7113774657585594
1111	1	0.4457727055250693
1111	2	0.8035325642566598
1111	3	0.5536987358004275
1111	4	1.018660765299856
1111	5	0.8109921590280251
1111	6	0.35710741804201973
1111	7	0.324384280127191
1111	8	0.09003810132426082
1111	9	0.3254867115971223
1111	10	0.5357292227646155
1111	11	0.23047179049950728
1111	12	0.5117481721105221
1111	13	0.25625478213336805
1111	14	-0.49202994053948146
1111	15	0.0644812231697216
1111	16	0.44466184027911027
1111	17	1.037020582909399
1111	18	1.5991576742167481
1111	19	0.7878923152266637
1111	20	0.5905176956977982
1111	21	0.5174348302593117
1111	22	0.7320956720304395
1111	23	0.6548306611894432
1111	24	0.5405040238765579
1111	25	0.18139291170803773
1112	1	0.15875892017676918
1112	2	0.6179612405795325
1112	3	0.38430239516278775
1112	4	0.7922367564065809
1112	5	0.6015501614041057
1112	6	0.04788178325041059
1112	7	0.11171336728912706
1112	8	0.14128218761008782
1112	9	0.1334847552817493
1112	10	0.4443055765085404
1112	11	0.1173965371829766
1112	12	0.06268899100308545
1112	13	0.08648946616725794
1112	14	0.34267416676004814
1112	15	0.17156245261894298
1112	16	0.6241554607526396
1112	17	0.8233619650770194
1112	18	0.8097854633942418
1112	19	0.4901116548932052
1112	20	0.192718325852141
1112	21	0.280876796433283
1112	22	0.32250120632340185
1112	23	1.935029594971887
1112	24	0.7785442169150798
1112	25	0.8179581501403916
1113	1	0.482146961773843
1113	2	0.893780649946919
1113	3	0.56613716489667
1113	4	0.7677598857606824
1113	5	0.8537226823886144
1113	6	0.4303537143031875
1113	7	0.3593573438447777
1113	8	0.2450556162308999
1113	9	0.39508981243113794
1113	10	0.6893547135930049
1113	11	0.33149707646039417
1113	12	0.34464442316173205
1113	13	0.3010693896919676
1113	14	0.9855090886161975
1113	15	0.6227266658163089
1113	16	0.9755337601531551
1113	17	1.340396154356938
1113	18	1.9324367872163464
1113	19	0.8932005333220303
1113	20	0.5952128920607649
1113	21	0.6064102398362365
1113	22	0.8934416325365433
1113	23	0.9515320731311903
1113	24	0.8649996008884031
1113	25	1.2495244758461084
1114	1	-0.5563807574355266
1114	2	-3.0166520578836327
1114	3	-1.1783392804400457
1114	4	-0.1577484476312847
1114	5	0.10301614803051962
1114	6	-0.13682673867621986
1114	7	-0.17456052695970214
1114	8	-0.08603814977117707
1114	9	-0.08374448991944661
1114	10	0.02656497587263118
1114	11	-0.26101846729548583
1114	12	-0.20464607171330584
1114	13	-0.1635101568908297
1114	14	0.6030639754886096
1114	15	-0.09044360368697113
1114	16	0.2528725940958836
1114	17	0.6449123454272865
1114	18	0.8110498456130273
1114	19	0.22786594288524498
1114	20	-0.034198247220508904
1114	21	-0.19231584842211902
1114	22	-0.04462561780763963
1114	23	1.6016171699664667
1114	24	0.4649175920871116
1114	25	0.37336738169390316
1115	1	0.13435063691232685
1115	2	0.43077908720018954
1115	3	0.20008003946388087
1115	4	0.3132496617877294
1115	5	0.3617818349582791
1115	6	-0.02965599355107429
1115	7	-0.21261752774785236
1115	8	-0.25913291838872965
1115	9	-0.2899242320647559
1115	10	-0.05678513084796322
1115	11	-0.05404708844245221
1115	12	-0.18322909781011276
1115	13	-0.4578916676551543
1115	14	0.5058276742565084
1115	15	-0.20650797722169045
1115	16	0.26331399860232096
1115	17	0.767938223670086
1115	18	1.2397272457491084
1115	19	0.36901475237260684
1115	20	-0.006256432131003464
1115	21	0.04727276090687127
1115	22	0.27019715562886404
1115	23	1.8286794886094668
1115	24	0.6717774411553066
1115	25	0.2464612900052643
1116	1	0.42177146022187273
1116	2	0.34905356992572223
1116	3	0.42868542744493254
1116	4	1.115734751382604
1116	5	0.6158924151465826
1116	6	0.45519283914231323
1116	7	0.2809941066580066
1116	8	0.34130060071117896
1116	9	0.32792348644127856
1116	10	0.8973114097849901
1116	11	0.4005409102101041
1116	12	0.475275053792366
1116	13	0.3067549071421922
1116	14	0.9998099440935206
1116	15	0.487795936767931
1116	16	0.84897611006609
1116	17	1.1742518588008792
1116	18	1.6435655630510055
1116	19	0.8806787678590879
1116	20	0.6129507980339639
1116	21	0.47224414096425704
1116	22	0.8783307233079825
1116	23	2.273206688923459
1116	24	1.3565053923941903
1116	25	1.4705253086116485
1201	1	0.9198264360792905
1201	2	1.3129427802078304
1201	3	1.1325677622928616
1201	4	1.648752786065458
1201	5	1.4976632774945813
1201	6	1.093632610767429
1201	7	0.9202209345640853
1201	8	0.9656852517329
1201	9	1.107906259764789
1201	10	1.426037059487335
1201	11	1.0156751502056096
1201	12	1.2209987384727654
1201	13	1.1632102208672066
1201	14	2.0282094391966794
1201	15	1.579571793804611
1201	16	1.8833628037202672
1201	17	2.3088844301704086
1201	18	2.916856215364966
1201	19	1.9889794738290547
1201	20	1.762665281821704
1201	21	1.639884510047466
1201	22	2.021875647652017
1201	23	3.6057610603235517
1201	24	2.27141046712169
1201	25	2.544996878643701
1202	1	1.1773494243843317
1202	2	1.3534841073015933
1202	3	1.0927042581650745
1202	4	1.688181340561684
1202	5	1.423827402721976
1202	6	1.1102059378781277
1202	7	0.8949835377852509
1202	8	0.8816815033348533
1202	9	1.0173018067695097
1202	10	1.5546025088241677
1202	11	1.210519356750602
1202	12	1.2993142769689783
1202	13	1.225831591392435
1202	14	2.0101386577583398
1202	15	1.4381724200897494
1202	16	1.765565532579437
1202	17	2.089372706108385
1202	18	2.700302095883984
1202	19	1.9890265669156832
1202	20	1.6737971879699387
1202	21	1.6207345561351971
1202	22	1.9768774096742519
1202	23	3.6086546394722205
1202	24	2.1721919477918235
1202	25	2.484215313311836
1203	1	0.6656332307994006
1203	2	1.0485395217543179
1203	3	0.729142016792931
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2211	16	-0.40444017773214114
2211	17	0.16684418883912144
2211	18	-5.371748186972134
2211	19	-1.8019735203948812
2211	20	-1.0655079370263394
2211	21	-0.8303768805785268
2211	22	-0.4395570142382752
2211	23	-4.811308251865604
2211	24	-2.2213133338282276
2211	25	-1.4406799558271572
2212	1	0.38164968697761026
2212	2	0.6390447210016532
2212	3	0.3133273037708548
2212	4	0.7477938800205592
2212	5	0.7126038264079
2212	6	0.1934673160286664
2212	7	0.3043494279916459
2212	8	0.04772205637859628
2212	9	0.043270725581628255
2212	10	-3.9929285483786465
2212	11	-1.161654417949272
2212	12	-0.28171787095016754
2212	13	0.0362087193763756
2212	14	0.8192495815904195
2212	15	0.4157439723580225
2212	16	0.6809431316368801
2212	17	1.1880195517967849
2212	18	1.765741969822649
2212	19	0.9994083021019691
2212	20	0.5471225044260777
2212	21	0.48621787927056204
2212	22	0.7708990555877975
2212	23	2.0224144547976053
2212	24	1.169390786017594
2212	25	1.0905289233326911
2213	1	-1.4602360956817897
2213	2	-3.5386143523960847
2213	3	-1.7255075436634009
2213	4	-3.4398782285118052
2213	5	-1.3690329329585893
2213	6	-1.1110949642180366
2213	7	-1.014744188504416
2213	8	-0.8401961176328179
2213	9	-0.9503366506965847
2213	10	-0.9530906089068552
2213	11	-1.0113373219116824
2213	12	-0.8478856265777139
2213	13	-1.183258369629435
2213	14	-6.254115675772713
2213	15	-2.852476764245612
2213	16	-2.037365508393883
2213	17	-0.8559959412086116
2213	18	-0.09030673000049916
2213	19	-0.633484393456051
2213	20	-0.7395127747680164
2213	21	-1.1334721340750349
2213	22	-0.9935722343404692
2213	23	-5.896985623610618
2213	24	-3.2760245139115654
2213	25	-2.3345576914290973
2214	1	-0.11563187578857637
2214	2	-0.042017779013704626
2214	3	-0.043746788684760674
2214	4	0.2343805066117639
2214	5	-0.07495563825471407
2214	6	-0.24650168539093897
2214	7	-0.3475133496380991
2214	8	-0.38299889324412817
2214	9	-0.38907919763864385
2214	10	0.07279959839623729
2214	11	-0.2853688479915846
2214	12	-0.2040051736632975
2214	13	-0.24345910663807047
2214	14	0.24393136163499207
2214	15	-0.10461303718818726
2214	16	0.30537473764003664
2214	17	0.482856974301356
2214	18	1.103131914582363
2214	19	0.23082215731409406
2214	20	0.0808312489925811
2214	21	-0.14514024337074716
2214	22	0.2850103148039693
2214	23	1.7008016439732287
2214	24	0.8027330504326002
2214	25	0.6762272117349823
2215	1	-2.402945744221782
2215	2	-4.3203181656534575
2215	3	-2.5201149265043377
2215	4	-3.823001844373664
2215	5	-4.910430999303102
2215	6	-2.506702253829644
2215	7	-1.8124126952576383
2215	8	-2.3342375669227344
2215	9	-1.8212718459643185
2215	10	-3.3576722522059548
2215	11	-2.261122785300657
2215	12	-1.9245545791013434
2215	13	-1.8649752252896583
2215	14	-1.1547560148978278
2215	15	-1.8092046913938091
2215	16	-2.577343464873337
2215	17	-7.018702059486305
2215	18	-3.242441386462559
2215	19	-2.449161280822331
2215	20	-1.9820531955031555
2215	21	-1.8873248784739332
2215	22	-1.7907470218637194
2215	23	-6.242283279463088
2215	24	-3.7057250326887043
2215	25	-3.4590181155796813
2216	1	-1.1484476442717195
2216	2	-0.6971503843492179
2216	3	-0.9186784892148232
2216	4	-3.337977115084371
2216	5	-1.4764206363244625
2216	6	-1.1644147699605156
2216	7	-1.2122783543207731
2216	8	-1.0729208815721982
2216	9	-1.0619720186496266
2216	10	-0.761145475442035
2216	11	-1.3469485220384598
2216	12	-1.943890698311641
2216	13	-1.3956798261412047
2216	14	-7.181796895328581
2216	15	-3.5479207154105445
2216	16	-2.0109974352673277
2216	17	-1.1491403502807955
2216	18	-0.369146236277345
2216	19	-0.8573753470439129
2216	20	-1.4018687007198736
2216	21	-1.5545334199903778
2216	22	-1.0113341116004761
2216	23	0.0688489971406554
2216	24	-0.6632743197756383
2216	25	-0.6152785140774384
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117826.0
3	0.0
4	972.0
5	5950.0
6	7063.0
7	6311.0
8	5917.0
9	5596.0
10	5212.0
11	5098.0
12	5100.0
13	5098.0
14	5120.0
15	5141.0
16	5185.0
17	5672.0
18	6334.0
19	7533.0
20	9340.0
21	11643.0
22	14284.0
23	17420.0
24	20678.0
25	23766.0
26	27137.0
27	31231.0
28	34778.0
29	41910.0
30	48986.0
31	56243.0
32	71860.0
33	92874.0
34	120417.0
35	156758.0
36	218296.0
37	299223.0
38	719985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20345975076997	17.48471883329165	12.060011670688322	25.251809745250053
2	14.313515713789121	18.985641546577618	41.315720273120405	25.38512246651285
3	19.73268705917947	23.438618512458905	28.271312685096223	28.557381743265402
4	11.075745065536234	15.6054752041001	38.318685187469555	35.00009454289411
5	13.056969251659517	37.34714973653027	35.69330538357087	13.902575628239344
6	33.83977067396035	35.28539261910828	17.470445192499007	13.404391514432362
7	29.637318854116824	30.55458898402554	20.841482919293103	18.966609242564537
8	26.339392427924924	35.95000267849225	19.593958581956684	18.11664631162614
9	26.594525338452286	15.394401806519461	19.136600026651205	38.87447282837705
10	14.494852081768789	28.88164920678657	34.109348014400126	22.514150697044517
11	35.40830510885555	22.508548370542826	22.583228448112877	19.499918072488743
12	23.49949808461888	24.505894692301116	30.70794826895462	21.286658954125386
13	29.198358085537457	20.889981017783505	25.117789222224275	24.793871674454763
14	21.298536669249078	20.973747197199653	27.65954860790686	30.068167525644412
15	24.63705680415518	28.691621853029275	22.5758095490186	24.09551179379695
16	23.037257048606484	27.661121294732403	26.681437919324114	22.620183737336998
17	22.741647875295826	27.5103868373819	27.366823615317195	22.381141672005082
18	22.514456409300717	26.743029278336405	28.723802681604283	22.018711630758595
19	23.806856833625144	26.88028071679308	27.09125051605662	22.221611933525153
20	23.907234445590856	26.69359529095699	27.07191050081299	22.32725976263917
21	23.55286123377468	26.327051267795348	27.02736284251948	23.092724655910487
22	23.40268716845994	26.671286053503845	26.56210463946721	23.363922138569
23	22.018406446352948	26.201206777872464	28.541471678355833	23.23891509741875
24	23.142935742557807	27.06721554385622	26.848487113347524	22.941361600238448
25	23.311292803790366	26.16424103788429	26.823431002277587	23.70103515604776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158.0
1	158.0
2	306.0
3	454.0
4	454.0
5	454.0
6	857.5
7	1261.0
8	1261.0
9	1261.0
10	2013.0
11	2765.0
12	2765.0
13	2765.0
14	5046.0
15	7327.0
16	7327.0
17	7327.0
18	13633.5
19	19940.0
20	19940.0
21	19940.0
22	31518.0
23	43096.0
24	43096.0
25	43096.0
26	61771.0
27	80446.0
28	80446.0
29	80446.0
30	105222.0
31	129998.0
32	129998.0
33	129998.0
34	154371.0
35	178744.0
36	178744.0
37	178744.0
38	203008.5
39	227273.0
40	227273.0
41	227273.0
42	249085.5
43	270898.0
44	270898.0
45	270898.0
46	295236.0
47	319574.0
48	319574.0
49	319574.0
50	310115.5
51	300657.0
52	300657.0
53	300657.0
54	270175.0
55	239693.0
56	239693.0
57	239693.0
58	207389.5
59	175086.0
60	175086.0
61	175086.0
62	143886.0
63	112686.0
64	112686.0
65	112686.0
66	87636.0
67	62586.0
68	62586.0
69	62586.0
70	46103.5
71	29621.0
72	29621.0
73	29621.0
74	20932.5
75	12244.0
76	12244.0
77	12244.0
78	8545.5
79	4847.0
80	4847.0
81	4847.0
82	3317.0
83	1787.0
84	1787.0
85	1787.0
86	1184.0
87	581.0
88	581.0
89	581.0
90	385.5
91	190.0
92	190.0
93	190.0
94	111.5
95	33.0
96	33.0
97	33.0
98	22.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12250462092650757
2	0.04973093538713846
3	0.036679377683726554
4	0.033438990943569114
5	0.03730945288320161
6	0.022097637353018083
7	0.0213325460393698
8	0.025112997236220146
9	0.030108593460629527
10	0.03892964625328033
11	0.021647583639107328
12	0.02101750843963227
13	0.01818217004199451
14	0.023312782380577123
15	0.024077873694225406
16	0.02362781998031465
17	0.02862341620472403
18	0.02029742249737506
19	0.035374221913385365
20	0.023807841465878953
21	0.029703545118109846
22	0.03973974293831969
23	0.030153598832020602
24	0.042215038364828844
25	0.03357400705774234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2221957.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.97541494662356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51512056956048	64.1153164891334
2	9.876570058659336	14.809998767947635
3	2.451919614527031	5.515010715447883
4	0.9116321358571903	2.7339999065827816
5	0.4036578064369384	1.5132205767026654
6	0.21522053646965397	0.9681749416108342
7	0.1421941035005643	0.7462743339042561
8	0.09049038534977326	0.5427643352223293
9	0.06763831322054181	0.45640895399998344
>10	0.2977897243554411	3.809695973582259
>50	0.016636507460918184	0.8524600680146174
>100	0.008639656197997331	1.2623865556896077
>500	0.001579397524542423	0.8315079361206296
>1k	7.896987622710685E-4	1.1328766173556244
>5k	1.2149211727247204E-4	0.7099038286854595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8750	0.39379699967191084	No Hit
CCCATGTACTCTGCGTTGATACCAC	6837	0.30770172420078334	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3702	0.1666098848897616	No Hit
GTATCAACGCAGAGTACATGGGAAG	2467	0.11102825122178332	No Hit
GCGTTGATACCACTGCTTCCCATGT	2387	0.10742782151049728	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2261	0.10175714471522176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.500537139107553E-5	0.0
10	0.0	0.0	0.0	4.500537139107553E-5	0.0
11	0.0	0.0	0.0	4.500537139107553E-5	0.0
12	0.0	0.0	0.0	9.001074278215105E-5	1.800214855643021E-4
13	0.0	0.0	0.0	1.3501611417322657E-4	1.800214855643021E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	135	4.7384674E-9	11.258339	6
GTCCTAC	900	0.0	10.990985	1
TAGGACC	415	0.0	10.75961	4
ACCGTGC	135	5.7010766E-8	10.554217	8
TACACCG	120	1.5237292E-6	10.291984	5
CTACACG	75	0.0026471647	10.133874	4
TTAGGAC	465	0.0	10.011287	3
GTAGGAC	855	0.0	9.889416	3
GTTAGAC	145	1.6164631E-7	9.828111	3
TAGGACG	605	0.0	9.736056	4
TATGGCG	590	0.0	9.660228	16
TGGCGAG	515	0.0	9.591664	18
ACTGTTC	565	0.0	9.582856	8
AGGACGT	595	0.0	9.580127	5
GTATTAA	270	0.0	9.511429	1
GTCCTAA	380	0.0	9.511429	1
GTCTTAC	250	0.0	9.511429	1
GGACGTG	590	0.0	9.499224	6
GGACGGT	90	0.0011147432	9.499223	6
TCCTACA	1090	0.0	9.413558	2
>>END_MODULE