FastQCFastQC Report
Thu 26 May 2016
SRR1513033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2221957
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC100480.4522139717375269No Hit
CCCATGTACTCTGCGTTGATACCAC94700.42620086707348526No Hit
GAGTACATGGGAAGCAGTGGTATCA37970.17088539517191378No Hit
GTATCAACGCAGAGTACTTTTTTTT33070.14883276319028677No Hit
CATGTACTCTGCGTTGATACCACTG27010.12155950812729498No Hit
GTATCAACGCAGAGTACATGGGAAG26180.11782406230183573No Hit
GCGTTGATACCACTGCTTCCCATGT25420.11440365407611398No Hit
ACGCAGAGTACATGGGAAGCAGTGG24140.10864296653805633No Hit
TATCAACGCAGAGTACTTTTTTTTT23060.10378238642782016No Hit
TATCAACGCAGAGTACATGGGAAGC22270.1002269620879252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG1102.8176146E-912.93298419
TTGCGCG604.1307247E-412.65298918
GTCCTAC7650.011.8366271
TAGGACC5700.011.6922494
ACCGCAT752.1029609E-411.3833318
GTAGGAC10250.011.3321113
GTGCGCC1105.072052E-711.20984811
TTTGCGC855.367896E-511.16767117
TGTAGGA11250.010.925862
TAGGACG6800.010.9209544
GTCCTAG3450.010.7748521
TCCTACA9750.010.65222552
AGGACGT6800.010.5009175
GGACGTG6600.010.3863626
CCTACAG10350.010.2107483
ATTTGCG856.6418614E-410.05113116
GACGTGG3900.09.9725777
ATAGGAC2201.8189894E-129.9535973
TGGACCG1151.0003852E-59.9347815
GACCGTG1252.6193738E-69.8655557
Produced by FastQC (version 0.11.5)