FastQCFastQC Report
Thu 26 May 2016
SRR1513032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844788
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC74570.4042198886809758No Hit
CCCATGTACTCTGCGTTGATACCAC59800.3241564884420324No Hit
GAGTACATGGGAAGCAGTGGTATCA31020.16814940253297397No Hit
GTATCAACGCAGAGTACTTTTTTTT21920.11882124124831689No Hit
GCGTTGATACCACTGCTTCCCATGT21580.11697821104647256No Hit
CATGTACTCTGCGTTGATACCACTG19730.10694995847761368No Hit
GTATCAACGCAGAGTACATGGGAAG19630.1064078907711889No Hit
GCTTCCCATGTACTCTGCGTTGATA19300.10461906733998703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA1750.014.11184913
TGCGTTT1900.012.99775512
TAGGACC3800.012.0018334
GTCCTAC8050.011.9340221
CGTTTAT2100.011.75987314
CGTGCAA658.034474E-411.69060610
AATCCCG1501.7644197E-1011.39957719
GTAGGAC7300.011.19288353
TGGCGAG4850.010.96866518
TCCTACA9750.010.9144872
AGGACGT5050.010.9116715
GACAACG700.001493443410.8555627
ATAGGAC1502.1300366E-910.7677273
GTGTAGG2750.010.722361
GGACGTG4800.010.6865236
TGTAGGA8400.010.6325752
GATATAC3600.010.5686341
GACGTGG3150.010.5540197
ACGTGGA3150.010.5540198
GTATTAC1451.41590135E-810.4957471
Produced by FastQC (version 0.11.5)