FastQCFastQC Report
Thu 26 May 2016
SRR1513032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844788
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC91560.4963171920025499No Hit
CCCATGTACTCTGCGTTGATACCAC78840.42736617974531493No Hit
GAGTACATGGGAAGCAGTGGTATCA35970.19498175400100173No Hit
GTATCAACGCAGAGTACTTTTTTTT33110.17947861759725237No Hit
TATCAACGCAGAGTACTTTTTTTTT24040.1303130766245227No Hit
CATGTACTCTGCGTTGATACCACTG23610.12798218548689605No Hit
GTATCAACGCAGAGTACATGGGAAG22720.1231577828997153No Hit
GCTTCCCATGTACTCTGCGTTGATA21280.11535200792719814No Hit
ACGCAGAGTACATGGGAAGCAGTGG21170.11475573345013086No Hit
GGTATCAACGCAGAGTACTTTTTTT20850.11302111678957148No Hit
CACACACACACACACACACACACAC20120.10906402253267043No Hit
GCGTTGATACCACTGCTTCCCATGT20110.10900981576202794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA759.453961E-713.9619836
GTATTAC1259.822543E-1112.95819951
TAGGACC4350.012.9115534
GTCCTAA4050.012.2335951
TTTGCGC550.003085969212.08191317
GTAGGAC7750.012.0376053
GACCGTG907.5816315E-611.5946097
TAGCACC1900.011.5236464
GTCCTAC7500.011.4337061
GTAATAC1751.8189894E-1211.4234423
TGGACCG752.0350119E-411.423445
TATACCG600.005787187311.1061235
TCTGGAC2500.011.042663
TGTAGGA9800.010.6921732
TCCTACA9800.010.497772
TCCAACG1002.4260702E-510.43939418
TATGGCA5850.010.38599416
GTGTAGC5000.010.2903351
TTTGCAC1951.4551915E-1110.2518063
TCTTATA2151.8189894E-1210.1903422
Produced by FastQC (version 0.11.5)