FastQCFastQC Report
Thu 26 May 2016
SRR1513031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582769
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC85670.5412666030229301No Hit
CCCATGTACTCTGCGTTGATACCAC64150.4053023530281425No Hit
GAGTACATGGGAAGCAGTGGTATCA38190.24128599940989492No Hit
GTATCAACGCAGAGTACTTTTTTTT25250.1595305442550366No Hit
GCGTTGATACCACTGCTTCCCATGT23350.14752626567742988No Hit
GTATCAACGCAGAGTACATGGGAAG23300.14721036360959813No Hit
CATGTACTCTGCGTTGATACCACTG22120.1397550748087687No Hit
GCTTCCCATGTACTCTGCGTTGATA20510.12958302822458614No Hit
ACGCAGAGTACATGGGAAGCAGTGG17380.10980755877831826No Hit
TATCAACGCAGAGTACATGGGAAGC16960.10715398140853151No Hit
ACTCTGCGTTGATACCACTGCTTCC15950.10077275963833003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG350.002172466616.28319514
TAGGACC4300.014.3623284
CGATTTT400.005284579514.24779614
GTAGGAC7800.013.5205573
ACCGTCC1102.752131E-912.9525428
GTCCTAC7900.012.7622031
TGTAGGA9150.012.2521812
CTGTAGG9050.012.1914771
AGGACCT6400.012.1734545
TCGTCCA550.003071317212.0894229
GGACCTG6000.012.0322336
GGACGTG5350.011.8961086
AGCACCG658.0148334E-411.6938265
TGCACCG658.0148334E-411.6938265
TCCTACA9650.011.4204492
TCTAGAC1501.7644197E-1011.4011183
ATACCGT752.0756232E-411.3989576
AGGACGT5700.011.3348045
TCGTACA600.005872828411.0840712
TAGGACG5750.011.0710014
Produced by FastQC (version 0.11.5)