##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513031_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1582769
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.920808406027664	31.0	30.0	34.0	22.0	34.0
2	28.65885546153608	31.0	29.0	34.0	16.0	34.0
3	29.011710489654522	31.0	30.0	34.0	20.0	34.0
4	31.84548029434491	35.0	32.0	37.0	17.0	37.0
5	32.054241016850845	35.0	32.0	37.0	19.0	37.0
6	32.5037317511273	36.0	33.0	37.0	25.0	37.0
7	32.47192799454627	36.0	33.0	37.0	24.0	37.0
8	32.445728972452706	37.0	33.0	37.0	23.0	37.0
9	33.76752893189088	39.0	34.0	39.0	22.0	39.0
10	33.26674960148954	38.0	33.0	39.0	17.0	39.0
11	33.621877229083964	38.0	34.0	39.0	21.0	39.0
12	33.45431266343983	38.0	34.0	39.0	17.0	39.0
13	33.535023114554306	38.0	34.0	39.0	18.0	39.0
14	33.80572149189174	39.0	33.0	41.0	10.0	41.0
15	34.34171568940256	39.0	33.0	41.0	17.0	41.0
16	33.92919118329965	39.0	33.0	41.0	11.0	41.0
17	33.46961116878079	39.0	32.0	41.0	10.0	41.0
18	32.88790657385885	38.0	32.0	41.0	10.0	41.0
19	33.76663177001824	39.0	33.0	41.0	11.0	41.0
20	34.02104729117136	39.0	34.0	41.0	10.0	41.0
21	34.04204909244495	39.0	34.0	41.0	10.0	41.0
22	33.6295504903116	39.0	33.0	41.0	2.0	41.0
23	31.97171349704221	38.0	30.0	41.0	2.0	41.0
24	33.03695864652391	39.0	32.0	41.0	2.0	41.0
25	32.76810576906674	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.116805353486157
1101	2	1.4208837832842072
1101	3	1.149929472387111
1101	4	1.1363103453299708
1101	5	1.3078843078582665
1101	6	1.1913626027467714
1101	7	1.2246548931245655
1101	8	1.1609066270906396
1101	9	1.3988170268577065
1101	10	1.6732294433361687
1101	11	1.227687252406568
1101	12	1.2837641555143477
1101	13	1.373565006017074
1101	14	0.49064249533756055
1101	15	0.9339272065716671
1101	16	1.541747896626248
1101	17	2.2246088277896376
1101	18	-4.051469324452988
1101	19	-0.26400378401701374
1101	20	0.6778487518743717
1101	21	1.1351947955532964
1101	22	1.584033403145547
1101	23	3.4185726365174247
1101	24	2.4743450758280616
1101	25	2.636856554823858
1102	1	0.8015377700282968
1102	2	1.0868095719995203
1102	3	0.8421154334059686
1102	4	1.3687491512097374
1102	5	1.2318069654009562
1102	6	0.7753868541952471
1102	7	0.7589387398004064
1102	8	0.7822812439316493
1102	9	0.839035318391609
1102	10	1.2348332164169875
1102	11	0.8932854969550661
1102	12	1.0432217039023612
1102	13	0.8130762011169139
1102	14	1.7857361010128585
1102	15	1.3404146827717298
1102	16	1.6581033755549512
1102	17	1.2495663040431282
1102	18	1.6030759519825324
1102	19	1.3206440303069442
1102	20	1.2946087184609496
1102	21	1.2724813975898073
1102	22	1.674678535133907
1102	23	3.1590646830183644
1102	24	2.1237468936180335
1102	25	2.5512268204392043
1103	1	0.7685173266650374
1103	2	1.0277640229014793
1103	3	0.7986215674119421
1103	4	1.2406970141173552
1103	5	1.2616455734263283
1103	6	0.822924243252956
1103	7	0.6402362706021876
1103	8	0.5824405493401272
1103	9	0.6857090257353988
1103	10	1.0798344662202553
1103	11	0.8110032208745963
1103	12	0.8417594696813921
1103	13	0.7057900260775156
1103	14	1.5758567444158587
1103	15	1.2330256037022664
1103	16	1.4755897256380237
1103	17	1.7648666022155695
1103	18	1.6814549963067549
1103	19	1.2319553552337155
1103	20	1.1044096685962899
1103	21	1.14566339671957
1103	22	1.447806839012003
1103	23	2.9690999267584886
1103	24	1.9339430806854523
1103	25	2.06438144496056
1104	1	0.5374831484437159
1104	2	0.907739672968976
1104	3	0.7506170142409125
1104	4	1.1686512833460263
1104	5	1.0803388077338028
1104	6	0.6519420171616588
1104	7	0.6008905225882373
1104	8	0.6088399695397726
1104	9	0.6803112470884827
1104	10	1.0626538856068422
1104	11	0.584197337639317
1104	12	0.7009262309721507
1104	13	0.6484765652049234
1104	14	1.5238078875449546
1104	15	1.0588608540495699
1104	16	1.2921952721517158
1104	17	1.7186988077699326
1104	18	2.2883525317347093
1104	19	1.3817873449049713
1104	20	0.9388052449551267
1104	21	0.980190395425673
1104	22	1.2325962618611257
1104	23	2.7663071843561013
1104	24	1.8720305385777323
1104	25	1.8481429698242664
1105	1	0.3594640966077165
1105	2	0.7622418837937168
1105	3	0.4741737259440377
1105	4	0.6425687786876075
1105	5	0.8316369113648037
1105	6	0.4806251363822014
1105	7	0.26440084489512117
1105	8	0.31156757584282957
1105	9	0.45557891369979586
1105	10	0.9775986303626638
1105	11	0.5947189979820209
1105	12	0.6279951508966306
1105	13	0.5283868612729989
1105	14	1.3057428767931896
1105	15	0.6740763915249488
1105	16	0.889554069430261
1105	17	1.5173543762399007
1105	18	1.2740908160035218
1105	19	1.0471779444412874
1105	20	0.7742945680143265
1105	21	0.5400815043638829
1105	22	0.9205199520217775
1105	23	2.3344973419324546
1105	24	1.479845911650564
1105	25	1.5488801163946704
1106	1	-0.5628722474836714
1106	2	-0.16323747745894224
1106	3	-0.4076363829157792
1106	4	-2.5200858262260155
1106	5	-0.5056664485538249
1106	6	-0.44629070089160194
1106	7	-0.5548207192672692
1106	8	-0.4671156192251402
1106	9	-0.4697409739267613
1106	10	-0.44232385505459604
1106	11	-0.4262854119837627
1106	12	-0.31505664388369325
1106	13	-0.4036614266476164
1106	14	0.09198997528324071
1106	15	-0.397161690638292
1106	16	-1.3098982080004618
1106	17	-6.283174625512402
1106	18	-7.066535662269324
1106	19	-3.153666433740714
1106	20	-1.8489002815422424
1106	21	-1.529414155093363
1106	22	-1.9156488050494467
1106	23	-5.745680277018835
1106	24	-2.7318364328336138
1106	25	-1.3230564949190509
1107	1	0.6228442647815058
1107	2	0.9046547519053227
1107	3	0.5784437879229962
1107	4	0.8231800830239244
1107	5	0.992439211233453
1107	6	0.6614053417209149
1107	7	0.5739678970012534
1107	8	0.479666466039248
1107	9	0.5874823861066858
1107	10	0.978062102756148
1107	11	0.6237037839896473
1107	12	0.6308116756479194
1107	13	0.7136111066767725
1107	14	1.446311406280408
1107	15	0.9947974446495067
1107	16	-5.3239868981948675
1107	17	-0.6357042818382794
1107	18	1.0165667203820945
1107	19	0.9207446527132248
1107	20	0.7619699892153875
1107	21	0.7847510272671414
1107	22	0.8779200361036459
1107	23	2.8116069833144053
1107	24	1.793350635935596
1107	25	1.333581103812044
1108	1	0.3266691429684272
1108	2	0.4226328925501832
1108	3	0.2648231004832091
1108	4	0.7561206864331744
1108	5	0.4314539065659986
1108	6	0.08089423323897904
1108	7	0.08231546598777584
1108	8	0.2930965011343005
1108	9	0.35639839444566235
1108	10	0.750775138186782
1108	11	0.3631168217398155
1108	12	0.3611616766408119
1108	13	0.2907568883872642
1108	14	0.9512160525740043
1108	15	0.5367216866666809
1108	16	0.774748822278255
1108	17	0.9250570793310189
1108	18	0.5333168429919937
1108	19	0.5819672006529046
1108	20	0.35347669008211113
1108	21	0.6220011746576759
1108	22	0.9900786828841746
1108	23	2.568110981673712
1108	24	1.3021823033484665
1108	25	1.4295196999065851
1109	1	0.013478325556928183
1109	2	0.3256425088507662
1109	3	0.17533654185782765
1109	4	0.1331205526664938
1109	5	-0.11295319816895955
1109	6	-0.09438347492097421
1109	7	-0.0716175003326498
1109	8	-0.21769950769664348
1109	9	-0.1427430042984028
1109	10	0.3384124855081936
1109	11	-0.007400466891674284
1109	12	-0.007686458335285806
1109	13	-0.10468134983026545
1109	14	-1.2151696036201898
1109	15	-0.4690326242951386
1109	16	-0.7118628744875863
1109	17	0.5128548127308505
1109	18	1.2624878729803442
1109	19	0.5070210296026616
1109	20	0.23799117982585472
1109	21	0.2294865503101633
1109	22	0.10959996224943325
1109	23	-4.317416384661318
1109	24	-1.5663878346722946
1109	25	-0.2501060949294853
1110	1	0.6925332567538405
1110	2	1.0571064753208539
1110	3	0.8780738290504395
1110	4	1.3667635926130863
1110	5	1.0601486897432082
1110	6	0.732950451862159
1110	7	0.5551860306936476
1110	8	0.5360837347646878
1110	9	0.7610439472241666
1110	10	1.0352430926815188
1110	11	0.8616418534970336
1110	12	0.9037875226001333
1110	13	0.7042463605662377
1110	14	1.5621012691219178
1110	15	0.9615801126211494
1110	16	1.4676556893639017
1110	17	1.8086339010435992
1110	18	2.330966416061088
1110	19	1.5234058076153474
1110	20	1.1721738881823711
1110	21	1.0653884085498433
1110	22	1.4350608131080804
1110	23	2.754418858838555
1110	24	1.9629693850154055
1110	25	2.187062407722493
1111	1	0.46260822390740586
1111	2	0.8313689235921125
1111	3	0.6278331894056706
1111	4	1.1548162733738572
1111	5	0.7896978329255546
1111	6	0.3504651537149073
1111	7	0.2122604849510381
1111	8	0.09566927249768753
1111	9	0.1340248576500116
1111	10	0.4836531145323022
1111	11	0.2787792604906869
1111	12	0.5146942949374704
1111	13	0.40634853229511947
1111	14	-0.33297281978477145
1111	15	0.028848554049105246
1111	16	0.6182434121751328
1111	17	1.0672143730486
1111	18	1.7154751950168858
1111	19	0.8869736679951714
1111	20	0.6578619770469061
1111	21	0.5646981065031795
1111	22	0.9419866679935964
1111	23	0.7891289319789507
1111	24	0.7626799148441776
1111	25	0.4587041673076797
1112	1	0.28161960899808847
1112	2	0.7021994775567144
1112	3	0.42363666220725804
1112	4	0.980553311665961
1112	5	0.7555472761192377
1112	6	0.2181663637697966
1112	7	0.23509196435897906
1112	8	0.18795316858901145
1112	9	0.1625234201322101
1112	10	0.586506365749301
1112	11	0.1888259475984242
1112	12	0.29384858935293323
1112	13	0.15501113463928107
1112	14	0.5227834440898107
1112	15	0.3004993387584207
1112	16	0.7509187921758809
1112	17	0.9815674837516184
1112	18	0.8402522430160886
1112	19	0.4390559469496935
1112	20	0.4323591916007601
1112	21	0.38310452701784214
1112	22	0.36067968045627197
1112	23	2.0226562605346032
1112	24	0.90598751976286
1112	25	1.0971673403838196
1113	1	0.23197521184291148
1113	2	0.594709079171988
1113	3	0.4140699702891055
1113	4	0.5475481553170205
1113	5	0.6843808606340716
1113	6	0.20983398941849885
1113	7	0.10289490678251667
1113	8	0.08405926051466395
1113	9	0.2370307854219007
1113	10	0.5850726619719779
1113	11	0.2721239985982109
1113	12	0.196091038061752
1113	13	0.0847598033978656
1113	14	0.8602018357446326
1113	15	0.4149253310894352
1113	16	0.6606725317442539
1113	17	0.9617630176235821
1113	18	1.6833026797272694
1113	19	0.7111879701597275
1113	20	0.3905974728916206
1113	21	0.3692128774231094
1113	22	0.6243934824743818
1113	23	0.6286266467592299
1113	24	0.41438881774247704
1113	25	0.9310327805381036
1114	1	-0.2126044957381481
1114	2	-2.6438655086699967
1114	3	-0.950234076218738
1114	4	0.13596191923090473
1114	5	0.3694953226818427
1114	6	0.14467920402927348
1114	7	0.031972980916542326
1114	8	-0.0035029494169762643
1114	9	0.033957915555923535
1114	10	0.30217681389953555
1114	11	0.01480118529872243
1114	12	0.01973792912367145
1114	13	0.025971710451607066
1114	14	0.6897660571640643
1114	15	0.12249068972550958
1114	16	0.5113228086445858
1114	17	0.8749651911654936
1114	18	0.7502075168527611
1114	19	0.2850616932249821
1114	20	0.16741591385424215
1114	21	-0.005220759555420784
1114	22	0.2431031868689999
1114	23	1.8140427715976628
1114	24	0.8162241833687958
1114	25	0.7367959058883926
1115	1	0.21861017098865076
1115	2	0.5634196066339854
1115	3	0.34431519002864164
1115	4	0.5156942218068714
1115	5	0.47873533859301887
1115	6	0.013876713051580225
1115	7	-0.002298487317190734
1115	8	-0.13435285014558218
1115	9	-0.006530445350229286
1115	10	0.2498877612735555
1115	11	0.2094998789866409
1115	12	-0.013586789511016661
1115	13	-0.11431981326309426
1115	14	0.8166398986605472
1115	15	0.2613954782259853
1115	16	0.633887710859085
1115	17	1.11301663305791
1115	18	1.5106868063077954
1115	19	0.8055749384686877
1115	20	0.39432606428191264
1115	21	0.41929554535172286
1115	22	0.6593700505752125
1115	23	2.3366368423723287
1115	24	1.0615278717317835
1115	25	0.6690048765070671
1116	1	0.0430409287950404
1116	2	0.021793226809634803
1116	3	0.021053567274631035
1116	4	0.6003028514662638
1116	5	0.0676622822153945
1116	6	0.10144697003807579
1116	7	0.006147594732958339
1116	8	-0.09063101010394803
1116	9	-0.09934016950513325
1116	10	0.40049603815086243
1116	11	-0.18480241823444032
1116	12	-0.038481335979170694
1116	13	-0.09509046789161602
1116	14	0.521623979913663
1116	15	-0.12406576256520196
1116	16	0.2508191818514689
1116	17	0.5082232065639758
1116	18	1.0293445140517932
1116	19	0.24348502041429754
1116	20	0.09900527213370935
1116	21	-0.09029353974497667
1116	22	0.2524032578216193
1116	23	1.6880412743605149
1116	24	0.5971477011333377
1116	25	0.8782955667622261
1201	1	0.9068558750794224
1201	2	1.2427658863323785
1201	3	0.9771932665686904
1201	4	1.5786770189445996
1201	5	1.3854850780892392
1201	6	0.8375228624019542
1201	7	0.7778593914322371
1201	8	0.731250749612947
1201	9	0.9479837679668037
1201	10	1.2380588709235383
1201	11	0.8393975423350071
1201	12	1.0410103812833356
1201	13	0.9608561950115018
1201	14	1.8344017386054858
1201	15	1.3015457437283615
1201	16	1.4929493973855585
1201	17	2.002031928763344
1201	18	2.379099713453094
1201	19	1.5496261279424104
1201	20	1.3554634180249039
1201	21	1.1928509509493779
1201	22	1.5259305931920935
1201	23	3.236734603385578
1201	24	2.0607747178096147
1201	25	2.226901121023026
1202	1	1.003801493187595
1202	2	1.2766590531961768
1202	3	1.0939269364637667
1202	4	1.618345316357587
1202	5	1.3763313144926812
1202	6	1.0634086147118182
1202	7	0.9641457205501425
1202	8	0.9061853055450513
1202	9	1.0484799335165675
1202	10	1.6647118929020053
1202	11	1.2278980420798433
1202	12	1.1893687072818366
1202	13	1.1557854253936028
1202	14	1.991926843801366
1202	15	1.386842399098576
1202	16	1.568906274530761
1202	17	1.944642890827751
1202	18	2.622653601565567
1202	19	1.9144132876183448
1202	20	1.5219942343579618
1202	21	1.5769250617804715
1202	22	1.8831643658561887
1202	23	3.387996493001353
1202	24	2.09711698689523
1202	25	2.437747888896837
1203	1	0.9823444655368974
1203	2	1.2834630644681404
1203	3	1.0260394089621627
1203	4	1.6217226074864222
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2211	16	-0.20495052496710287
2211	17	0.38012560950423335
2211	18	-5.063369780399395
2211	19	-1.6009855376701054
2211	20	-0.793881721817435
2211	21	-0.5968980578900798
2211	22	-0.1902663327961065
2211	23	-4.208959054729593
2211	24	-1.8798276404068588
2211	25	-1.286642939984123
2212	1	0.5120454331739133
2212	2	0.6613095638989428
2212	3	0.3720929678064202
2212	4	1.1023481834608369
2212	5	0.9338815906142699
2212	6	0.46820724550710935
2212	7	0.3359472103035088
2212	8	0.2245096180918864
2212	9	0.19209726890248646
2212	10	-3.7744466693108762
2212	11	-0.9408018650651044
2212	12	-0.08385778165700941
2212	13	0.1863919561986762
2212	14	0.8475397889354355
2212	15	0.514196356026865
2212	16	0.5591916205379945
2212	17	1.1680127349290075
2212	18	1.7970729520689375
2212	19	0.9653837857775329
2212	20	0.45383028003612935
2212	21	0.38747887291004446
2212	22	0.6646108683160321
2212	23	1.888416987902474
2212	24	1.0382726453515616
2212	25	0.9107466182488082
2213	1	-1.4612206543226804
2213	2	-3.6453294634285953
2213	3	-1.6944358493131801
2213	4	-3.290502827940788
2213	5	-1.3044619488874538
2213	6	-1.0954343999519338
2213	7	-1.0308772827333215
2213	8	-1.014843177022282
2213	9	-0.9324765273124598
2213	10	-0.8317795204232681
2213	11	-1.0524028764667293
2213	12	-0.9250806977556181
2213	13	-1.255924888208071
2213	14	-6.1701125587988805
2213	15	-2.85968266963436
2213	16	-2.01959927356528
2213	17	-0.8634251986911323
2213	18	-0.07580328756709775
2213	19	-0.7770237590866884
2213	20	-0.8978485936371285
2213	21	-1.0508014224313484
2213	22	-0.9150988649728689
2213	23	-5.615189885891976
2213	24	-2.9608085154628156
2213	25	-2.247681896914642
2214	1	-0.4934173509756299
2214	2	-0.2248081093917378
2214	3	-0.49723755474372666
2214	4	-0.07877534358066995
2214	5	-0.45976856392966425
2214	6	-0.6541485851481177
2214	7	-0.7784913148706885
2214	8	-0.7443369718679165
2214	9	-0.615786836659133
2214	10	-0.28357157963117174
2214	11	-0.6586132993507618
2214	12	-0.6491883742310378
2214	13	-0.7154219694629873
2214	14	-0.13583245679187428
2214	15	-0.4732398853860005
2214	16	-0.12225238861179832
2214	17	0.15062423874715591
2214	18	0.654219472741957
2214	19	-0.25128054675973743
2214	20	-0.3841200226635948
2214	21	-0.43990473388497264
2214	22	-0.054195949307384694
2214	23	1.298069288616965
2214	24	0.3182571479608711
2214	25	0.10057030548529866
2215	1	-2.2499412276060298
2215	2	-4.066295401938756
2215	3	-2.2304976732674753
2215	4	-3.867894909288843
2215	5	-4.851711828559079
2215	6	-2.266200927630127
2215	7	-1.6159407332514917
2215	8	-1.9915269700690423
2215	9	-1.679598837415611
2215	10	-3.2477245009698734
2215	11	-2.0543339115219723
2215	12	-1.6310500374785946
2215	13	-1.6573103951132921
2215	14	-0.9891698158337761
2215	15	-1.4967435414862393
2215	16	-2.366354439458341
2215	17	-6.746799101005099
2215	18	-3.0725866251967275
2215	19	-2.2590047428309816
2215	20	-1.8776673725120503
2215	21	-1.8849544611470606
2215	22	-1.6546262942830054
2215	23	-6.666903440477327
2215	24	-3.8664931583310214
2215	25	-3.6062838672611655
2216	1	-0.9925330675936124
2216	2	-0.4994345446348021
2216	3	-0.8154532258373344
2216	4	-3.2133604981015047
2216	5	-1.3405592886284374
2216	6	-1.0057102242182872
2216	7	-1.0194142727256548
2216	8	-0.9384659987709263
2216	9	-0.9847062375504123
2216	10	-0.6315110987838466
2216	11	-1.1629915148462189
2216	12	-1.9002833530368513
2216	13	-1.4379133624664036
2216	14	-6.974114547581703
2216	15	-3.4506325142898113
2216	16	-1.9160530281060488
2216	17	-0.9194362743949966
2216	18	-0.22636232708280346
2216	19	-0.6465576264687556
2216	20	-1.2442107541329506
2216	21	-1.408363876819898
2216	22	-0.934595311682827
2216	23	0.08698391893891255
2216	24	-0.6467089127245131
2216	25	-0.42203239135947257
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87022.0
3	1.0
4	714.0
5	4417.0
6	5412.0
7	4880.0
8	4491.0
9	4047.0
10	3902.0
11	3845.0
12	3684.0
13	3636.0
14	3700.0
15	3706.0
16	3781.0
17	3948.0
18	4552.0
19	5374.0
20	6661.0
21	8280.0
22	10157.0
23	12524.0
24	14610.0
25	17060.0
26	19474.0
27	21892.0
28	24668.0
29	29409.0
30	34813.0
31	39186.0
32	50387.0
33	64860.0
34	84767.0
35	109901.0
36	154066.0
37	212791.0
38	516151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33548221417376	17.549263810122596	12.202573343841596	24.91268063186205
2	13.768987603815125	18.972464932469205	42.4960762165309	24.76247124718477
3	20.216181642131023	23.571851132735176	28.13085423476856	28.08111299036524
4	10.766392564545455	15.868345028652108	38.889826368282776	34.47543603851966
5	12.544558435385845	37.7791064548424	36.116792399405384	13.55954271036638
6	33.246379998710864	35.64461110500247	17.783915908036736	13.325092988249926
7	29.380186597440417	30.73025016999022	20.704492544443912	19.185070688125457
8	25.803868731827208	36.944752173783904	19.605353711089442	17.64602538329945
9	26.49385860411617	15.725702223668787	19.440369842538843	38.3400693296762
10	14.392564239991101	29.612208884888812	34.11197882431462	21.883248050805467
11	35.10995142900837	22.943442536965396	22.444019499174054	19.50258653485218
12	23.407808943392556	25.071692990959544	30.983821207981084	20.536676857666823
13	29.258328472858963	21.25052053942735	24.91575002227498	24.57540096543871
14	20.67463432082194	21.961541912970063	27.976400374621303	29.387423391586694
15	24.673776008341626	29.16195080335561	22.62995086654976	23.534322321753006
16	22.0680890648244	28.29496751133406	27.869726832313997	21.767216591527543
17	22.500290707962364	28.007879449727746	27.71761386904491	21.77421597326498
18	21.88138451923326	27.085938749059206	29.67864204731804	21.354034684389497
19	23.412470184637204	27.346265226849564	27.699435862367107	21.541828726146132
20	23.363540995209746	27.142658527028907	27.988283471732455	21.505517006028892
21	22.87295157080471	26.68193969576126	27.84346935809039	22.601639375343645
22	22.635428734675404	27.556047703831236	27.120825140518235	22.687698420975124
23	21.292574531500033	26.994142644430458	29.06899140735797	22.64429141671154
24	22.422365037910485	27.78709703516991	27.67901612238552	22.11152180453409
25	22.69653434184348	26.737505909239147	27.31787535360634	23.248084395311032
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	256.5
3	370.0
4	370.0
5	370.0
6	756.0
7	1142.0
8	1142.0
9	1142.0
10	1884.0
11	2626.0
12	2626.0
13	2626.0
14	4390.5
15	6155.0
16	6155.0
17	6155.0
18	10924.0
19	15693.0
20	15693.0
21	15693.0
22	24876.5
23	34060.0
24	34060.0
25	34060.0
26	48543.0
27	63026.0
28	63026.0
29	63026.0
30	81556.5
31	100087.0
32	100087.0
33	100087.0
34	118076.0
35	136065.0
36	136065.0
37	136065.0
38	152671.5
39	169278.0
40	169278.0
41	169278.0
42	183890.5
43	198503.0
44	198503.0
45	198503.0
46	218142.0
47	237781.0
48	237781.0
49	237781.0
50	225827.0
51	213873.0
52	213873.0
53	213873.0
54	186697.0
55	159521.0
56	159521.0
57	159521.0
58	135518.0
59	111515.0
60	111515.0
61	111515.0
62	90093.0
63	68671.0
64	68671.0
65	68671.0
66	52720.5
67	36770.0
68	36770.0
69	36770.0
70	26762.0
71	16754.0
72	16754.0
73	16754.0
74	11784.5
75	6815.0
76	6815.0
77	6815.0
78	4718.0
79	2621.0
80	2621.0
81	2621.0
82	1746.5
83	872.0
84	872.0
85	872.0
86	584.5
87	297.0
88	297.0
89	297.0
90	202.0
91	107.0
92	107.0
93	107.0
94	61.0
95	15.0
96	15.0
97	15.0
98	12.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11315612069733486
2	0.04738531017476334
3	0.036707820282050004
4	0.03234837174597177
5	0.03645509862778459
6	0.0196491086191352
7	0.020533634409064114
8	0.02464036129087694
9	0.02994751603045043
10	0.03645509862778459
11	0.021670881853258436
12	0.020659995236196816
13	0.017437794144312912
14	0.023439933433116265
15	0.021734062266824786
16	0.023439933433116265
17	0.026851675765699228
18	0.020723175649763167
19	0.03354879960373245
20	0.02457718087731059
21	0.028999809826955165
22	0.03727644400414716
23	0.030642500579680296
24	0.03923503682470405
25	0.03165338719674191
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1582769.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.95125453664018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.23421412018025	67.12782215065761
2	9.164875979355097	14.10497408568187
3	2.019441404731754	4.661956486720301
4	0.6708744958414793	2.064985363665513
5	0.3094440798218717	1.1906055075614626
6	0.15318139383889048	0.7072500256544273
7	0.09378661988198814	0.5051898641068991
8	0.06800877176161962	0.4186688245242127
9	0.04337321895682386	0.3003861250817982
>10	0.21550267094234782	2.8710885554547043
>50	0.014382674807233277	0.7666602625283762
>100	0.009415373377840795	1.4152051362336615
>500	0.0019995408587726205	1.043924688555114
>1k	0.001333027239181747	1.860767067532005
>5k	1.6662840489771837E-4	0.9605158560420513
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8567	0.5412666030229301	No Hit
CCCATGTACTCTGCGTTGATACCAC	6415	0.4053023530281425	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3819	0.24128599940989492	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2525	0.1595305442550366	No Hit
GCGTTGATACCACTGCTTCCCATGT	2335	0.14752626567742988	No Hit
GTATCAACGCAGAGTACATGGGAAG	2330	0.14721036360959813	No Hit
CATGTACTCTGCGTTGATACCACTG	2212	0.1397550748087687	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2051	0.12958302822458614	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1738	0.10980755877831826	No Hit
TATCAACGCAGAGTACATGGGAAGC	1696	0.10715398140853151	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1595	0.10077275963833003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.2636082713270226E-4	0.0
10	0.0	0.0	0.0	1.2636082713270226E-4	0.0
11	0.0	0.0	0.0	1.2636082713270226E-4	0.0
12	0.0	6.318041356635113E-5	0.0	1.2636082713270226E-4	1.2636082713270226E-4
13	0.0	6.318041356635113E-5	0.0	1.2636082713270226E-4	1.2636082713270226E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTG	35	0.0021724666	16.283195	14
TAGGACC	430	0.0	14.362328	4
CGATTTT	40	0.0052845795	14.247796	14
GTAGGAC	780	0.0	13.520557	3
ACCGTCC	110	2.752131E-9	12.952542	8
GTCCTAC	790	0.0	12.762203	1
TGTAGGA	915	0.0	12.252181	2
CTGTAGG	905	0.0	12.191477	1
AGGACCT	640	0.0	12.173454	5
TCGTCCA	55	0.0030713172	12.089422	9
GGACCTG	600	0.0	12.032233	6
GGACGTG	535	0.0	11.896108	6
AGCACCG	65	8.0148334E-4	11.693826	5
TGCACCG	65	8.0148334E-4	11.693826	5
TCCTACA	965	0.0	11.420449	2
TCTAGAC	150	1.7644197E-10	11.401118	3
ATACCGT	75	2.0756232E-4	11.398957	6
AGGACGT	570	0.0	11.334804	5
TCGTACA	60	0.0058728284	11.084071	2
TAGGACG	575	0.0	11.071001	4
>>END_MODULE