FastQCFastQC Report
Thu 26 May 2016
SRR1513031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582769
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC101420.6407757543899331No Hit
CCCATGTACTCTGCGTTGATACCAC89090.5628743044626221No Hit
GAGTACATGGGAAGCAGTGGTATCA40860.2581551698321107No Hit
GTATCAACGCAGAGTACTTTTTTTT38400.24261278809478828No Hit
CATGTACTCTGCGTTGATACCACTG27650.17469384351096084No Hit
GTATCAACGCAGAGTACATGGGAAG26010.1643322556860793No Hit
TATCAACGCAGAGTACTTTTTTTTT26000.16426907527251292No Hit
GCGTTGATACCACTGCTTCCCATGT24320.15365476579336593No Hit
GCTTCCCATGTACTCTGCGTTGATA24170.15270705958987066No Hit
GGTATCAACGCAGAGTACTTTTTTT24120.1523911575220389No Hit
ACGCAGAGTACATGGGAAGCAGTGG23790.15030620387434931No Hit
TATCAACGCAGAGTACATGGGAAGC21600.13646969330331843No Hit
ACTCTGCGTTGATACCACTGCTTCC18740.118400095023342No Hit
GGTATCAACGCAGAGTACATGGGAA16160.10209954832322342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT250.00595206919.0439974
CCCGTTT402.7798727E-416.61028112
ACTGCGC704.511403E-714.9064018
TAGGACC4050.014.8119964
TAGACTG1750.014.1469685
CCAACGA655.5291683E-513.1343119
TTAGACT1102.6539055E-912.9845424
GTAGGAC8550.012.9187343
GTCCTAC9150.012.7076861
CCGGTTT604.1227834E-412.65545312
GCCGGTT604.1459323E-412.64705511
GGACCGA550.003015784812.1189066
TGTAGGA9950.011.9714222
CTGCGCA658.124218E-411.6749429
AGGACGT6000.011.5850965
GGACGTG5800.011.4920676
TCCAACG1001.9553208E-611.38738718
TAAGCTA1104.8214315E-711.253275
GGCGTGT855.403953E-511.1598718
ATTTCTC9150.011.09945415
Produced by FastQC (version 0.11.5)