FastQCFastQC Report
Thu 26 May 2016
SRR1513030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890825
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC91400.483386881387754No Hit
CCCATGTACTCTGCGTTGATACCAC74670.39490698504620997No Hit
GAGTACATGGGAAGCAGTGGTATCA39590.20937950365581162No Hit
GCGTTGATACCACTGCTTCCCATGT25160.13306360979995505No Hit
CATGTACTCTGCGTTGATACCACTG24460.12936152208691973No Hit
GTATCAACGCAGAGTACATGGGAAG24090.1274047042957439No Hit
GCTTCCCATGTACTCTGCGTTGATA21480.1136012058228551No Hit
ACGCAGAGTACATGGGAAGCAGTGG19480.1030238123570399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA602.5712021E-514.24861118
CGACGGG400.005285858614.2474814
TTGCGCG707.2718485E-613.57010618
GGACCGC852.7041278E-713.4101046
GTCTTAG2700.013.3871261
GTATACG550.003040212712.1060631
GCGTTTA1459.822543E-1111.791018513
GTATAGA1900.011.5144131
TATCGAC600.005880719511.08225319
CGTTTAT1201.2787496E-711.08137414
GTGTTAC1951.8189894E-1210.7313811
TGCGTTT1605.456968E-1010.68532812
GGACCGT1451.4462785E-810.481466
CGTGCGC1002.4031102E-510.44842910
ACTGTCC4550.010.4379918
GTGTAGC3100.010.4324131
GTAGGAC2650.010.3985153
GTCCTAG2750.010.3766251
AGGACCG1752.3464963E-1010.3157375
TATACTG3050.010.2801355
Produced by FastQC (version 0.11.5)