##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513030_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1890825
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.066215011965678	31.0	28.0	34.0	16.0	34.0
2	27.811208863855725	31.0	28.0	34.0	10.0	34.0
3	28.161903930823847	31.0	28.0	34.0	16.0	34.0
4	30.94368384170931	35.0	32.0	37.0	10.0	37.0
5	31.10971560033319	35.0	32.0	37.0	10.0	37.0
6	31.57693440693877	35.0	32.0	37.0	17.0	37.0
7	31.530422963521215	35.0	32.0	37.0	17.0	37.0
8	31.507087646926607	35.0	32.0	37.0	15.0	37.0
9	32.746355691298774	37.0	33.0	39.0	11.0	39.0
10	32.188060767125464	37.0	32.0	39.0	10.0	39.0
11	32.57863207859003	37.0	32.0	39.0	10.0	39.0
12	32.42204540346145	37.0	32.0	39.0	10.0	39.0
13	32.48366982666296	37.0	32.0	39.0	8.0	39.0
14	32.70736794785345	38.0	32.0	41.0	7.0	41.0
15	33.17908161781233	38.0	32.0	41.0	2.0	41.0
16	32.71114672166911	38.0	32.0	41.0	2.0	41.0
17	32.20176483809977	38.0	31.0	40.0	2.0	41.0
18	31.539679240543148	38.0	29.0	40.0	2.0	41.0
19	32.43502809620139	38.0	31.0	40.0	2.0	41.0
20	32.709091534118706	38.0	32.0	40.0	2.0	41.0
21	32.73257070326445	39.0	32.0	41.0	2.0	41.0
22	32.29050229397221	38.0	32.0	41.0	2.0	41.0
23	30.514717649703172	38.0	27.0	40.0	2.0	41.0
24	31.527989105284732	38.0	30.0	40.0	2.0	41.0
25	31.258061428212553	38.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6813078087906028
1101	2	1.096419311787706
1101	3	0.853029968030075
1101	4	0.9010290582483869
1101	5	1.1542736150809674
1101	6	0.8470710495851534
1101	7	0.7757538325773048
1101	8	0.7476504002607101
1101	9	0.836728254380148
1101	10	1.258852215252702
1101	11	0.9230946846211125
1101	12	0.9650037881334512
1101	13	0.9824477977115222
1101	14	0.08888293621780718
1101	15	0.6331672764790852
1101	16	1.3052123962506172
1101	17	1.9705765892991352
1101	18	-4.404842659427814
1101	19	-0.8466808549105593
1101	20	0.3278324107490249
1101	21	0.8100633672536262
1101	22	1.3679426403820258
1101	23	3.317579789649379
1101	24	2.2491644582497514
1101	25	2.466898541213766
1102	1	1.2032215878640358
1102	2	1.4490653885300482
1102	3	1.291296313686292
1102	4	1.9949026250344737
1102	5	1.7725928929371904
1102	6	1.2503315772293107
1102	7	1.2581580156966332
1102	8	1.1739508713641058
1102	9	1.452967825196346
1102	10	1.745797779264521
1102	11	1.3202901422286786
1102	12	1.4578527954650369
1102	13	1.402109918709236
1102	14	2.308432710154804
1102	15	1.8241261638099786
1102	16	1.9763124727649242
1102	17	1.635958708392522
1102	18	2.105071490397517
1102	19	1.7883623901317023
1102	20	1.8251894840784075
1102	21	1.886835837847734
1102	22	2.223767540813917
1102	23	3.776764260818304
1102	24	2.811292833623078
1102	25	3.0888366082747822
1103	1	1.0385468421608088
1103	2	1.2131080087785442
1103	3	1.0882068726072838
1103	4	1.5307285356825382
1103	5	1.3212104457692462
1103	6	1.0551365790868488
1103	7	0.937141418509416
1103	8	0.9000169161126479
1103	9	1.0529637157512184
1103	10	1.3164409637663468
1103	11	1.1353061210106858
1103	12	1.2348600522811637
1103	13	0.9281580012056878
1103	14	1.8537736433103689
1103	15	1.3854008076387387
1103	16	1.6175463137804371
1103	17	2.1455627136433293
1103	18	2.065278805276126
1103	19	1.603975232677982
1103	20	1.615703227686815
1103	21	1.6077557967930503
1103	22	1.8528013008998698
1103	23	3.317312056482116
1103	24	2.3091540994183433
1103	25	2.3609643961107594
1104	1	0.6266648582869117
1104	2	1.1442946515930963
1104	3	0.9497349636601484
1104	4	1.5273581278631916
1104	5	1.2177778150363139
1104	6	0.7562928882310231
1104	7	0.7384740259087614
1104	8	0.8419733082207088
1104	9	1.0464717917668764
1104	10	1.3843469034619744
1104	11	0.9497625779342798
1104	12	1.021860648584088
1104	13	0.8544186633611943
1104	14	1.7806202379021627
1104	15	1.3388554756535669
1104	16	1.6684887316591528
1104	17	2.209571943375515
1104	18	2.6971006403335487
1104	19	1.8980666485085322
1104	20	1.3388449662987227
1104	21	1.422323736436823
1104	22	1.8487075517100635
1104	23	3.3221939625773658
1104	24	2.4680869764503086
1104	25	2.4755167800261333
1105	1	0.32884813763164544
1105	2	0.6117085774755253
1105	3	0.4698604611422006
1105	4	0.5612948116667233
1105	5	0.6285679036272818
1105	6	0.32875672937855427
1105	7	0.15251997227216663
1105	8	0.12537852289523954
1105	9	0.3749088682977728
1105	10	0.9469730609670108
1105	11	0.4232243957416415
1105	12	0.3665812863251361
1105	13	0.4018854349012244
1105	14	1.0952230136137544
1105	15	0.5404917849145932
1105	16	0.9051370418359994
1105	17	1.370762920614851
1105	18	1.23141142845029
1105	19	0.910069974367083
1105	20	0.5074656661531307
1105	21	0.37844464169258174
1105	22	0.9440633999518866
1105	23	2.4500301717120863
1105	24	1.5913284396966176
1105	25	1.4702225740967734
1106	1	-0.6107287730535731
1106	2	-0.24711623599490906
1106	3	-0.31607147617796727
1106	4	-2.6226359405868926
1106	5	-0.6166828930082389
1106	6	-0.6612316478533309
1106	7	-0.6190519702494228
1106	8	-0.4911797212043396
1106	9	-0.6051212856581003
1106	10	-0.41115789424936366
1106	11	-0.3708310789575009
1106	12	-0.2705615682642488
1106	13	-0.2857215037260019
1106	14	0.5041310766689477
1106	15	-0.16626345192221237
1106	16	-1.23642076657735
1106	17	-6.108671907204183
1106	18	-7.165594245301605
1106	19	-3.4877660726584345
1106	20	-1.8176674192337714
1106	21	-1.4066361434721024
1106	22	-1.686622584669955
1106	23	-5.096738175928888
1106	24	-2.5670147952580216
1106	25	-1.230943590376345
1107	1	0.15226791835591058
1107	2	0.5424628676062255
1107	3	0.350110449751142
1107	4	0.5761713196924774
1107	5	0.6074230893101387
1107	6	0.20517147740403274
1107	7	0.21297081748874191
1107	8	0.11028193101409656
1107	9	-0.0432687256182831
1107	10	0.5189991563785199
1107	11	0.07639663371075045
1107	12	0.2509885815891195
1107	13	0.20969785296082222
1107	14	1.085956924495271
1107	15	0.3546063600538858
1107	16	-5.910187564656649
1107	17	-1.3835740638752547
1107	18	0.378341258667632
1107	19	0.41094773068095236
1107	20	0.2108575366337888
1107	21	0.16035207235972138
1107	22	0.2777062262068597
1107	23	2.2583189724528054
1107	24	1.2298712266631782
1107	25	0.896232595649515
1108	1	0.48184880477520764
1108	2	0.6446290997536295
1108	3	0.3932132245297133
1108	4	1.0628096328799188
1108	5	0.8749684504878168
1108	6	0.3954233230136346
1108	7	0.2220860660425359
1108	8	0.44431444534445674
1108	9	0.6290804874190528
1108	10	1.0177917195154862
1108	11	0.6042776281014994
1108	12	0.6242017857078324
1108	13	0.43334842924748074
1108	14	1.0077105389055063
1108	15	0.6592568134462056
1108	16	0.9728365577461133
1108	17	0.9339866590945789
1108	18	0.6098096704338865
1108	19	0.6418793575386275
1108	20	0.6669386117836709
1108	21	0.8440628668716101
1108	22	1.2132560466933455
1108	23	2.7733645311392365
1108	24	1.6045418259486368
1108	25	1.7450819974174365
1109	1	0.2856024257472569
1109	2	0.6185255205598956
1109	3	0.46257542368705273
1109	4	0.37653840849822373
1109	5	0.18749906390874216
1109	6	0.3112567505421069
1109	7	0.23130699465148652
1109	8	0.05609393562684062
1109	9	0.2909999405996331
1109	10	0.868029645360636
1109	11	0.44180718875320224
1109	12	0.39730164807210855
1109	13	0.31556527311182947
1109	14	-0.9328423533286028
1109	15	-0.030433140475743414
1109	16	-0.22566673686748118
1109	17	0.8364574530598787
1109	18	1.6180565104639406
1109	19	0.9275410839471832
1109	20	0.7574885119368204
1109	21	0.4545801422605251
1109	22	0.34949856926139233
1109	23	-4.333717392037421
1109	24	-1.5186951180506902
1109	25	-0.23443288652306649
1110	1	0.5317083929947302
1110	2	0.9596933787144408
1110	3	0.7117264119476872
1110	4	1.27822952760636
1110	5	0.9927732287218731
1110	6	0.72825427851933
1110	7	0.4306039103336339
1110	8	0.46496979439826447
1110	9	0.5536786557156219
1110	10	0.9206832143717563
1110	11	0.5886686361028595
1110	12	0.7415987347503545
1110	13	0.37540422705451704
1110	14	1.3211110441056846
1110	15	0.9575126027862026
1110	16	1.3461667864973634
1110	17	1.5708366372213831
1110	18	2.222480598845042
1110	19	1.3977745355581916
1110	20	0.9783338391943133
1110	21	0.9596125895759826
1110	22	1.223426375355693
1110	23	2.5694330012858586
1110	24	1.6703420319799918
1110	25	2.0585121372647492
1111	1	0.27149407041477147
1111	2	0.6419261006856978
1111	3	0.45241822480384997
1111	4	0.8889689617904928
1111	5	0.6509057277508248
1111	6	0.1351787920634706
1111	7	0.16299620365973055
1111	8	-0.1969849680005744
1111	9	0.15072421788673296
1111	10	0.39166857679932576
1111	11	0.17303468715078907
1111	12	0.3138613065148945
1111	13	0.14107473003151227
1111	14	-0.6040379122157162
1111	15	-0.06276961344123322
1111	16	0.4345581433593537
1111	17	1.0267198133245259
1111	18	1.7767574197622444
1111	19	0.8933503811693129
1111	20	0.6912568309842868
1111	21	0.624396218480797
1111	22	0.8688391378715892
1111	23	0.6496362565627827
1111	24	0.6890881278292227
1111	25	0.16500523882321616
1112	1	0.15313341467706465
1112	2	0.41340151052176566
1112	3	0.28651658545958725
1112	4	0.8758542076921323
1112	5	0.6295942523409899
1112	6	-0.07334579810046193
1112	7	-9.382225697756041E-4
1112	8	-0.0678046401317225
1112	9	0.06383897198509203
1112	10	0.3929800522091611
1112	11	0.016969113380298495
1112	12	0.11451704421981646
1112	13	7.468611790528712E-4
1112	14	0.3566178254892023
1112	15	0.0664107144276187
1112	16	0.44144338926099636
1112	17	0.614916534635995
1112	18	0.5272492230409362
1112	19	0.25957988936603016
1112	20	0.1374863391810095
1112	21	0.2847240873332595
1112	22	0.13539651492077098
1112	23	1.922095272956227
1112	24	0.9025307507800449
1112	25	0.9177921240691163
1113	1	0.6784526021417818
1113	2	1.113431975268938
1113	3	0.8519235454825953
1113	4	1.0903013151389516
1113	5	1.1884272181799957
1113	6	0.6118753908768397
1113	7	0.6287152675611374
1113	8	0.5279360473489696
1113	9	0.7602326276482287
1113	10	1.1322629589295161
1113	11	0.9387068822103899
1113	12	0.6686062441473837
1113	13	0.6325348009739002
1113	14	1.2947731104837175
1113	15	0.8893851815608471
1113	16	1.2786091025728155
1113	17	1.8175126423917263
1113	18	2.4308231852689097
1113	19	1.4729754304348575
1113	20	1.095284994897412
1113	21	1.1181232500853113
1113	22	1.2888598027700198
1113	23	1.2186067398444536
1113	24	1.1457819571201213
1113	25	1.7192827662787735
1114	1	-0.4384877868588397
1114	2	-3.142145668690464
1114	3	-1.1364790765332948
1114	4	-0.02767555450275694
1114	5	0.16621678073300217
1114	6	0.02727777011348209
1114	7	0.05338608272627354
1114	8	0.10143569412770148
1114	9	0.1707636444682592
1114	10	0.34102155843455506
1114	11	0.14549684467298363
1114	12	0.06796941550123137
1114	13	0.10915457969059617
1114	14	0.9213003819347421
1114	15	0.1866068725022174
1114	16	0.6342520457785668
1114	17	0.9282325376038258
1114	18	1.071510218345491
1114	19	0.44780108981878897
1114	20	0.2827810907881272
1114	21	0.21370067509260338
1114	22	0.32824091531720256
1114	23	2.0972895626007926
1114	24	1.0212748408546162
1114	25	0.9799974130800422
1115	1	-0.08033732738631372
1115	2	0.15150909424642123
1115	3	-0.22144168577737133
1115	4	-0.06317062148425734
1115	5	-0.05822567286217151
1115	6	-0.4383048847916875
1115	7	-0.6050048086283226
1115	8	-0.6377768022102863
1115	9	-0.6981979765288102
1115	10	-0.4805463656970872
1115	11	-0.44182401430858675
1115	12	-0.6621024760681244
1115	13	-0.8227258492222056
1115	14	-0.031469231283288934
1115	15	-0.6339762045612147
1115	16	-0.18550428107052852
1115	17	0.2852576194713592
1115	18	0.7549251783877828
1115	19	0.04016982834755822
1115	20	-0.46546729240237994
1115	21	-0.4242976487407546
1115	22	-0.19726214717185542
1115	23	1.6063710652122225
1115	24	0.3853882149984713
1115	25	-0.11468038949835346
1116	1	-0.10503650366738526
1116	2	-0.22629680026798837
1116	3	-0.16437289905078956
1116	4	0.7549329336162103
1116	5	-0.07737077913691337
1116	6	-0.17169648064113474
1116	7	-0.24546356072348985
1116	8	-0.307860739365033
1116	9	-0.2614443291431243
1116	10	0.13150027909939155
1116	11	-0.23384183220344568
1116	12	-0.11715533625765318
1116	13	-0.27554560282393936
1116	14	0.3693062254144053
1116	15	-0.349456642051841
1116	16	0.02587647534215165
1116	17	0.34956965437669396
1116	18	0.9272454830920474
1116	19	0.04114942124909504
1116	20	-0.22128321763504744
1116	21	-0.18373193710107927
1116	22	0.11775392936943518
1116	23	1.6183179245799835
1116	24	0.6595998775019751
1116	25	0.777935488776464
1201	1	1.1588029246508533
1201	2	1.5938120129874456
1201	3	1.2390473509311768
1201	4	1.8239440828531777
1201	5	1.658284109731369
1201	6	1.2488466582503293
1201	7	1.1006007174097867
1201	8	1.1325969769992028
1201	9	1.3349267590924683
1201	10	1.633225731715136
1201	11	1.1249023845162895
1201	12	1.2907694321535317
1201	13	1.2389746811621762
1201	14	2.2259447613815126
1201	15	1.6265719832534273
1201	16	1.9967326069072726
1201	17	2.4399274191710703
1201	18	3.0995045431351222
1201	19	2.2077810533448883
1201	20	1.7645646189802022
1201	21	1.7545041752980737
1201	22	1.9603423143787637
1201	23	3.6899414678116145
1201	24	2.6372715211385653
1201	25	2.6366408068182423
1202	1	1.2273245621067943
1202	2	1.5744192724024195
1202	3	1.3889787726240854
1202	4	1.9147675788892933
1202	5	1.6703978481066173
1202	6	1.2433877514386609
1202	7	1.074470612199672
1202	8	1.1000288331976407
1202	9	1.2671272284504056
1202	10	1.8055931569503016
1202	11	1.4762350520676009
1202	12	1.5908353896335967
1202	13	1.4515290403331846
1202	14	2.402874034915847
1202	15	1.7632708722986408
1202	16	2.087112171629286
1202	17	2.5278563956783486
1202	18	3.1701467515083444
1202	19	2.5156073026287586
1202	20	2.0072602131400785
1202	21	1.9635758231913414
1202	22	2.5080263079306064
1202	23	4.001059153979995
1202	24	2.7803010868261495
1202	25	2.9957553765014353
1203	1	1.0630588990287784
1203	2	1.3674164573235963
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2211	12	-0.8915562181774241
2211	13	-0.5848742239388827
2211	14	-0.44987191315125585
2211	15	-0.6275183889064451
2211	16	-0.7913823154849844
2211	17	-0.119160104305017
2211	18	-5.659676240292917
2211	19	-2.196410733735334
2211	20	-1.4352202187337078
2211	21	-1.2702260441261295
2211	22	-0.9320419895069136
2211	23	-5.2332327303127855
2211	24	-2.7969225502216304
2211	25	-2.178585941300341
2212	1	0.32218366052320846
2212	2	0.6484024539494015
2212	3	0.3295215689773521
2212	4	0.8593324589102629
2212	5	0.8304304622036938
2212	6	0.26761272438086436
2212	7	0.23052697604948946
2212	8	0.020136356793813093
2212	9	-0.10924315061610201
2212	10	-4.011184126398337
2212	11	-1.2069699744916598
2212	12	-0.223217619531475
2212	13	0.1555762117678654
2212	14	0.8580489497219759
2212	15	0.4737146202180469
2212	16	0.6919460697054092
2212	17	1.1747782392432526
2212	18	1.7635962712223439
2212	19	0.9236694816943043
2212	20	0.4047509539850509
2212	21	0.2454135664502104
2212	22	0.5282468872972075
2212	23	1.9538732455857257
2212	24	1.2705811853117126
2212	25	1.201392243939921
2213	1	-1.5174299663934647
2213	2	-3.784306207894115
2213	3	-1.8223672378474411
2213	4	-3.4205778802174933
2213	5	-1.3627285534188367
2213	6	-1.10764018557267
2213	7	-1.0015609157542045
2213	8	-0.8704408087595894
2213	9	-0.8389803416970203
2213	10	-0.8188296113528146
2213	11	-0.9509818463109845
2213	12	-0.8648602625957587
2213	13	-1.2693995988566762
2213	14	-6.50789437577993
2213	15	-3.1061720788985063
2213	16	-2.203218576495921
2213	17	-1.2035186112919654
2213	18	-0.28487777993306906
2213	19	-0.7868141693411985
2213	20	-0.8349845508367473
2213	21	-1.1953862477605846
2213	22	-1.0761716436033169
2213	23	-5.552177047497214
2213	24	-3.3495611913976475
2213	25	-2.3939184059313874
2214	1	0.19749726013822055
2214	2	0.30132547793016684
2214	3	0.1874687287584571
2214	4	0.46120556549447755
2214	5	0.4111667643024539
2214	6	0.11291799419863224
2214	7	-0.0029479653305166664
2214	8	0.013701039528907444
2214	9	0.3174790540065544
2214	10	0.6029361121412435
2214	11	-0.08465525871070412
2214	12	0.07597086375090356
2214	13	-0.013364084780086216
2214	14	0.38949606267877357
2214	15	0.30032355035215375
2214	16	0.686712725603229
2214	17	0.8615774149562725
2214	18	1.3620603892428882
2214	19	0.4164854236447084
2214	20	0.34923211605225646
2214	21	0.2568608765619729
2214	22	0.7772363560855595
2214	23	2.16012892779343
2214	24	1.2457712494184392
2214	25	0.9793446731355289
2215	1	-2.629734350987082
2215	2	-4.555590287036953
2215	3	-2.6525144511139196
2215	4	-4.422079051662884
2215	5	-5.193904998342546
2215	6	-2.6962696195000255
2215	7	-2.0611205753090722
2215	8	-2.415563211511266
2215	9	-1.9883983382548038
2215	10	-3.80249338995619
2215	11	-2.714214577832678
2215	12	-2.242146381128819
2215	13	-2.282853518627153
2215	14	-1.432512395416154
2215	15	-2.1165375884563886
2215	16	-2.9304845848389647
2215	17	-7.1173722553609124
2215	18	-3.806726958991483
2215	19	-2.8478993185321073
2215	20	-2.3679366528224683
2215	21	-2.3694639624991467
2215	22	-2.125273408317195
2215	23	-7.097267979649224
2215	24	-4.592756449064375
2215	25	-4.428208127609693
2216	1	-1.4466746883866826
2216	2	-1.0276479932279088
2216	3	-1.4092009055957782
2216	4	-3.6776669798049184
2216	5	-1.8986515212772233
2216	6	-1.5262753932167925
2216	7	-1.4798195874097857
2216	8	-1.412963891679766
2216	9	-1.572505014053867
2216	10	-1.013402272789321
2216	11	-1.6968932841185698
2216	12	-2.8309090303154356
2216	13	-1.9773504753076416
2216	14	-7.482720642165248
2216	15	-3.9904445475777273
2216	16	-2.478983662277617
2216	17	-1.613689180496774
2216	18	-0.6747031665745276
2216	19	-1.1615495265207159
2216	20	-1.7225531452307337
2216	21	-2.0110016240262603
2216	22	-1.4854583967109605
2216	23	-0.4088400224693771
2216	24	-1.1014280668437522
2216	25	-0.955449573086792
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	134256.0
3	1.0
4	1127.0
5	6439.0
6	7573.0
7	6773.0
8	6208.0
9	5932.0
10	5666.0
11	5323.0
12	5265.0
13	5209.0
14	5373.0
15	5471.0
16	5603.0
17	5970.0
18	6613.0
19	7689.0
20	9459.0
21	11985.0
22	14643.0
23	17685.0
24	20808.0
25	23740.0
26	26617.0
27	30118.0
28	33712.0
29	39868.0
30	46536.0
31	53729.0
32	67409.0
33	84742.0
34	107090.0
35	136160.0
36	184290.0
37	243135.0
38	512608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.575684786274465	16.30521161308153	11.548966778395394	25.570136822248614
2	15.411918026463455	17.960905368302583	39.53740012180364	27.08977648343031
3	20.635406862597275	21.916621829168868	27.41891026098863	30.029061047245232
4	11.9872331713232	14.34294901468672	36.01291072795576	37.65690708603431
5	14.146670511148557	36.34463840267068	34.648481209213	14.860209876967762
6	36.47639385313953	33.08790818747384	16.162195358698224	14.273502600688403
7	31.47251352173396	29.592509818960842	19.28631693093006	19.648659728375144
8	28.152967053429073	34.64669270004031	18.24503251703084	18.955307729499776
9	27.032664621843495	15.126789264360932	17.989400635454018	39.85114547834156
10	15.831544263991509	27.95048307517477	32.28816061098878	23.929812049844937
11	37.060991698290465	21.866810829567925	20.7898737551932	20.282323716948415
12	24.418142722254697	23.651607681748573	29.00233012687422	22.92791946912251
13	30.131925060683624	19.65472353710019	24.122487560452207	26.090863841763976
14	22.64999605910392	19.623969746613607	25.905599405004036	31.820434789278433
15	26.138631040166565	26.8921641025207	21.380184804710893	25.589020052601846
16	24.744990935891067	26.424615020024618	24.798312335387372	24.032081708696943
17	24.526260717027107	26.327160346880678	25.82560850389852	23.320970432193693
18	24.48925723704325	25.52867469777549	26.752754059546717	23.229314005634542
19	25.114749276270125	25.889954967918875	25.77922641317781	23.21606934263319
20	25.076702856308568	25.453922213781972	25.55538157819565	23.913993351713806
21	24.72881417799947	25.159590310367246	26.032780883971878	24.078814627661405
22	24.909637470611017	25.017776235289634	25.047297483181353	25.025288810917996
23	23.682890778992274	24.784771207323228	27.127609487101083	24.404728526583412
24	24.463136018945058	26.1797145860768	24.969353424718413	24.387795970259727
25	24.789817718583222	24.823992839095492	25.114692974103086	25.271496468218196
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	104.0
2	194.0
3	284.0
4	284.0
5	284.0
6	515.0
7	746.0
8	746.0
9	746.0
10	1048.0
11	1350.0
12	1350.0
13	1350.0
14	2372.0
15	3394.0
16	3394.0
17	3394.0
18	6565.5
19	9737.0
20	9737.0
21	9737.0
22	15719.0
23	21701.0
24	21701.0
25	21701.0
26	32561.5
27	43422.0
28	43422.0
29	43422.0
30	58674.0
31	73926.0
32	73926.0
33	73926.0
34	93734.0
35	113542.0
36	113542.0
37	113542.0
38	136855.0
39	160168.0
40	160168.0
41	160168.0
42	185198.0
43	210228.0
44	210228.0
45	210228.0
46	243175.0
47	276122.0
48	276122.0
49	276122.0
50	276697.0
51	277272.0
52	277272.0
53	277272.0
54	258735.5
55	240199.0
56	240199.0
57	240199.0
58	214169.0
59	188139.0
60	188139.0
61	188139.0
62	159202.5
63	130266.0
64	130266.0
65	130266.0
66	103172.5
67	76079.0
68	76079.0
69	76079.0
70	56944.0
71	37809.0
72	37809.0
73	37809.0
74	27184.5
75	16560.0
76	16560.0
77	16560.0
78	11516.5
79	6473.0
80	6473.0
81	6473.0
82	4398.5
83	2324.0
84	2324.0
85	2324.0
86	1524.5
87	725.0
88	725.0
89	725.0
90	463.0
91	201.0
92	201.0
93	201.0
94	117.0
95	33.0
96	33.0
97	33.0
98	27.0
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10704322187404969
2	0.04749249666151019
3	0.03522272024116457
4	0.032472597940052625
5	0.03543426811048087
6	0.01925085610778364
7	0.018510438565176576
8	0.022794282918831725
9	0.02765988391310671
10	0.034006319992595825
11	0.01962106487908717
12	0.019568177911758095
13	0.014914124786799413
14	0.020731691192997767
15	0.020996126029643146
16	0.021260560866288525
17	0.026020387925905355
18	0.018140229793873044
19	0.03263125884203985
20	0.021842317506908362
21	0.02728967514180318
22	0.03495828540451919
23	0.0270252403051578
24	0.03850171221556728
25	0.0296695886716116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1890825.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.37347849639167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.94431514184323	60.75342086812516
2	10.36768903214599	15.00691438450587
3	2.8715377239875535	6.23469521155772
4	1.1820808633269115	3.4220521577194534
5	0.5577424533888954	2.0182880728432973
6	0.30547126650607626	1.3264810882645826
7	0.18334380819191606	0.9288460411736936
8	0.12919929981659617	0.7480482197620226
9	0.08698107958858249	0.5665610963877485
>10	0.34412353907733995	4.187260512305553
>50	0.016684940223926956	0.8172306604140218
>100	0.008309644577695756	1.1106333917637372
>500	0.0015572162890111095	0.786815095713275
>1k	8.156847228153383E-4	1.201501695848082
>5k	1.483063132391524E-4	0.8912515036157359
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	9140	0.483386881387754	No Hit
CCCATGTACTCTGCGTTGATACCAC	7467	0.39490698504620997	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3959	0.20937950365581162	No Hit
GCGTTGATACCACTGCTTCCCATGT	2516	0.13306360979995505	No Hit
CATGTACTCTGCGTTGATACCACTG	2446	0.12936152208691973	No Hit
GTATCAACGCAGAGTACATGGGAAG	2409	0.1274047042957439	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2148	0.1136012058228551	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1948	0.1030238123570399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0577393465815186E-4
2	0.0	0.0	0.0	0.0	1.0577393465815186E-4
3	0.0	0.0	0.0	0.0	1.0577393465815186E-4
4	0.0	0.0	0.0	0.0	1.0577393465815186E-4
5	0.0	0.0	0.0	0.0	1.0577393465815186E-4
6	0.0	0.0	0.0	0.0	1.0577393465815186E-4
7	0.0	0.0	0.0	0.0	1.0577393465815186E-4
8	0.0	0.0	0.0	0.0	1.0577393465815186E-4
9	0.0	0.0	0.0	0.0	1.0577393465815186E-4
10	0.0	0.0	0.0	0.0	1.0577393465815186E-4
11	0.0	0.0	0.0	0.0	1.0577393465815186E-4
12	0.0	0.0	0.0	0.0	1.586609019872278E-4
13	0.0	0.0	0.0	0.0	2.1154786931630373E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGA	60	2.5712021E-5	14.248611	18
CGACGGG	40	0.0052858586	14.24748	14
TTGCGCG	70	7.2718485E-6	13.570106	18
GGACCGC	85	2.7041278E-7	13.410104	6
GTCTTAG	270	0.0	13.387126	1
GTATACG	55	0.0030402127	12.106063	1
GCGTTTA	145	9.822543E-11	11.7910185	13
GTATAGA	190	0.0	11.514413	1
TATCGAC	60	0.0058807195	11.082253	19
CGTTTAT	120	1.2787496E-7	11.081374	14
GTGTTAC	195	1.8189894E-12	10.731381	1
TGCGTTT	160	5.456968E-10	10.685328	12
GGACCGT	145	1.4462785E-8	10.48146	6
CGTGCGC	100	2.4031102E-5	10.448429	10
ACTGTCC	455	0.0	10.437991	8
GTGTAGC	310	0.0	10.432413	1
GTAGGAC	265	0.0	10.398515	3
GTCCTAG	275	0.0	10.376625	1
AGGACCG	175	2.3464963E-10	10.315737	5
TATACTG	305	0.0	10.280135	5
>>END_MODULE