FastQCFastQC Report
Thu 26 May 2016
SRR1513030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890825
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC111010.587098224320072No Hit
CCCATGTACTCTGCGTTGATACCAC103950.5497600253857443No Hit
GAGTACATGGGAAGCAGTGGTATCA43350.22926500337154418No Hit
CATGTACTCTGCGTTGATACCACTG32490.17182975685216773No Hit
GCGTTGATACCACTGCTTCCCATGT27640.14617957769756587No Hit
ACGCAGAGTACATGGGAAGCAGTGG26660.14099665489931645No Hit
GTATCAACGCAGAGTACATGGGAAG26480.14004468948739307No Hit
GCTTCCCATGTACTCTGCGTTGATA26240.13877540227149524No Hit
ACTCTGCGTTGATACCACTGCTTCC22230.1175677283725358No Hit
TATCAACGCAGAGTACATGGGAAGC22020.1164571020586252No Hit
GTATCAACGCAGAGTACTTTTTTTT19960.10556238678883557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA701.0621357E-412.2495831
TCTATAC1604.3655746E-1111.3045423
CTAGACA2200.011.2504534
ACTGCGT855.400955E-511.1607428
GCGTGAC600.00593837411.06773511
TGCGTAT600.005943051511.06656410
TACCGTG1053.524774E-610.842157
TTACACG803.700275E-410.7095664
ACCGTGC1605.5842975E-1010.6724598
GTCTTAC909.242215E-510.586061
TAGACAG3250.010.2518925
ACCGTCT750.002679139110.1190738
GTACCGT1803.8016879E-1010.0484826
CTTACAC3550.09.9218513
GGTCTAC2500.09.9085511
TATACTG4450.09.8404995
TCTAGTA1659.778887E-99.814342
AGGACCG1752.4829205E-99.7916035
ATAGACC1752.4829205E-99.7916033
CTTATAC2054.0017767E-119.7517993
Produced by FastQC (version 0.11.5)