##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513030_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1890825 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.493025531183477 31.0 31.0 34.0 27.0 34.0 2 30.62742559464784 31.0 31.0 34.0 27.0 34.0 3 30.651626670897624 31.0 31.0 34.0 27.0 34.0 4 33.894818928245606 37.0 35.0 37.0 30.0 37.0 5 33.84415480015337 37.0 35.0 37.0 28.0 37.0 6 33.81838086549522 37.0 35.0 37.0 29.0 37.0 7 33.73381196038766 37.0 35.0 37.0 28.0 37.0 8 33.73412981106131 37.0 35.0 37.0 28.0 37.0 9 35.2826750228075 39.0 35.0 39.0 29.0 39.0 10 35.19076752211336 39.0 35.0 39.0 28.0 39.0 11 35.215623339018684 39.0 35.0 39.0 29.0 39.0 12 35.126555339600436 39.0 35.0 39.0 28.0 39.0 13 35.10793912710061 39.0 35.0 39.0 28.0 39.0 14 36.279628733489346 40.0 36.0 41.0 28.0 41.0 15 36.255784115399365 40.0 36.0 41.0 27.0 41.0 16 36.204414475162956 40.0 36.0 41.0 27.0 41.0 17 36.093655943834044 40.0 36.0 41.0 27.0 41.0 18 36.01060701016752 40.0 36.0 41.0 27.0 41.0 19 36.06100300133539 40.0 36.0 41.0 27.0 41.0 20 35.95720703925535 40.0 36.0 41.0 27.0 41.0 21 35.97582272288551 40.0 36.0 41.0 27.0 41.0 22 35.947783110546986 40.0 36.0 41.0 27.0 41.0 23 35.88337577512461 40.0 36.0 41.0 27.0 41.0 24 35.75148519826002 40.0 36.0 41.0 27.0 41.0 25 35.73526899633758 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6262579376102906 1101 2 0.5590920126238323 1101 3 0.6355174809070299 1101 4 0.882015045217166 1101 5 0.696064303822638 1101 6 0.7240923114783442 1101 7 0.5531728076148212 1101 8 0.648201980172459 1101 9 0.5988776947173022 1101 10 0.691559960905451 1101 11 0.619023425365917 1101 12 0.6280669715975264 1101 13 0.6648089508372266 1101 14 0.7191630248771048 1101 15 0.6140249669905415 1101 16 0.6237310233535283 1101 17 0.7432645973940026 1101 18 0.6200175637995571 1101 19 0.7159771973766951 1101 20 0.7199441506051869 1101 21 0.6637536737022813 1101 22 0.627892952857998 1101 23 0.6979106139509383 1101 24 0.778633973497719 1101 25 0.8413739675222587 1102 1 0.6472309282466071 1102 2 0.5973742350813467 1102 3 0.5915663387943759 1102 4 0.8528138253422668 1102 5 0.7224750989040416 1102 6 0.58725803350832 1102 7 0.700526565947257 1102 8 0.680115230385681 1102 9 0.6811731811208119 1102 10 0.6435295583702683 1102 11 0.6782883140727236 1102 12 0.6500214872006111 1102 13 0.7334146543252089 1102 14 0.7818827671187734 1102 15 0.797481955757263 1102 16 0.7941378092127493 1102 17 0.8758294011863157 1102 18 0.8416844278267348 1102 19 0.783410392491696 1102 20 0.780419810336312 1102 21 0.8436563040506542 1102 22 0.8320703767722222 1102 23 0.7647066479611624 1102 24 0.8382442156178413 1102 25 0.7662339267681375 1103 1 0.13222432827888397 1103 2 0.21673953128120615 1103 3 0.5190425860358268 1103 4 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2214 8 -0.012760200112964526 2214 9 -0.1253143035350348 2214 10 -0.11065319539373064 2214 11 -0.1792298402423711 2214 12 -0.16058145276048919 2214 13 -0.12978802347016227 2214 14 -0.16238337165900418 2214 15 -0.17801522313039442 2214 16 -0.18211489113112833 2214 17 -0.23294952820312176 2214 18 -0.20686373672032232 2214 19 -0.20858418198356077 2214 20 -0.07637351625958644 2214 21 -0.08643919793944832 2214 22 -0.09404730366627945 2214 23 -0.13576820475771 2214 24 -0.06139767632241444 2214 25 -0.10879310649671936 2215 1 -0.2621314969406541 2215 2 -0.18256830031882743 2215 3 -0.2507056400312777 2215 4 0.06457753156650625 2215 5 -0.21606620966672097 2215 6 -0.3250036961980882 2215 7 -0.5516336743775483 2215 8 -0.5442376695935778 2215 9 -0.790936909195409 2215 10 -0.716962682970987 2215 11 -0.7858413696501145 2215 12 -0.724978941656758 2215 13 -0.7759065319377214 2215 14 -0.9233759162136366 2215 15 -0.8974576188116572 2215 16 -1.0024018493435918 2215 17 -0.8687748297946598 2215 18 -0.8029220446487386 2215 19 -0.9371229322416141 2215 20 -0.7439617030064127 2215 21 -0.8486156633388049 2215 22 -0.8449348401918613 2215 23 -0.9145227948467607 2215 24 -0.8502173254828165 2215 25 -0.8791972098319718 2216 1 -1.127930012295213 2216 2 -0.7599842910189274 2216 3 -2.4886794297090873 2216 4 -0.8715230842679276 2216 5 -0.8480697811680216 2216 6 -0.7918269100387221 2216 7 -0.6899827413041919 2216 8 -0.7834303072298923 2216 9 -0.8930122348310263 2216 10 -0.9827965920984809 2216 11 -0.8873462073249172 2216 12 -0.8024468470481096 2216 13 -0.9572165833963666 2216 14 -1.1646379709626942 2216 15 -1.1762078170770138 2216 16 -1.0789950925748215 2216 17 -1.2519409605353573 2216 18 -1.2284908618743202 2216 19 -1.1388099302502113 2216 20 -1.063674887244197 2216 21 -1.119276348056296 2216 22 -1.2684961937484047 2216 23 -1.2613606882116954 2216 24 -1.2452003585570708 2216 25 -1.1650129688950273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45335.0 3 0.0 4 700.0 5 5432.0 6 6336.0 7 5012.0 8 3945.0 9 3616.0 10 3308.0 11 3107.0 12 2913.0 13 2729.0 14 2810.0 15 2729.0 16 2887.0 17 3078.0 18 3492.0 19 4117.0 20 5197.0 21 7007.0 22 8532.0 23 10819.0 24 12470.0 25 14283.0 26 15829.0 27 17342.0 28 19656.0 29 24150.0 30 29977.0 31 37888.0 32 48477.0 33 62206.0 34 82558.0 35 111836.0 36 160552.0 37 262583.0 38 857917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62312986013823 17.514247927718657 12.759021921356101 28.10360029078701 2 14.911392659617311 18.382708986790526 37.16433225781357 29.541566095778588 3 18.271230811947166 22.051961445400817 27.864292041833593 31.812515700818427 4 11.312469424722012 14.973199529305992 34.32692078854468 39.38741025742731 5 14.330012081766688 36.59511058730928 34.142363836796704 14.932513494127331 6 36.82356694694496 33.36589296833983 15.743875293766651 14.066664790948563 7 31.173633636079483 29.55368697849538 19.40202125763498 19.87065812779015 8 28.102739091907047 35.18464733034427 17.874115515348702 18.838498062399978 9 26.62509774129005 14.855141853164277 17.860441838245176 40.6593185673005 10 15.658441851043643 28.27713612674544 32.0730787578896 23.991343264321323 11 37.21971030803901 21.667926296576997 20.617082908682033 20.495280486701958 12 24.115201871969592 23.780022415820344 29.013095641715005 23.09168007049506 13 30.123654773685686 19.379351308294904 24.355425889381895 26.14156802863751 14 22.523082846501847 19.534714972196923 25.347551668660532 32.59465051264069 15 26.030965319371173 27.024447000647868 21.37315721973213 25.571430460248834 16 24.84073737905879 26.496888798617153 24.39997503422216 24.2623987881019 17 24.57075477437283 26.744898968605334 25.16667521886124 23.517671038160596 18 24.775353084621766 25.98638253374202 25.648750656212428 23.589513725423785 19 25.004642157135038 25.966401891778222 25.718079548851097 23.310876402235643 20 25.07291790620496 25.436727354461674 25.483796755384553 24.006557983948806 21 24.600531514021657 25.25897425726865 25.8749487657504 24.265545462959288 22 24.708896910078934 25.089101318207664 24.859307445162827 25.34269432655058 23 24.00006346452862 25.321553611660548 25.340698744460077 25.337684179350756 24 24.32102245532496 26.164031391654735 25.119564464325283 24.395381688695018 25 24.58307881480306 24.91737733529015 24.935411791149367 25.564132058757423 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 36.0 1 36.0 2 116.5 3 197.0 4 197.0 5 197.0 6 471.5 7 746.0 8 746.0 9 746.0 10 1043.0 11 1340.0 12 1340.0 13 1340.0 14 2260.5 15 3181.0 16 3181.0 17 3181.0 18 6159.5 19 9138.0 20 9138.0 21 9138.0 22 15349.5 23 21561.0 24 21561.0 25 21561.0 26 32077.0 27 42593.0 28 42593.0 29 42593.0 30 57468.0 31 72343.0 32 72343.0 33 72343.0 34 91680.5 35 111018.0 36 111018.0 37 111018.0 38 133620.5 39 156223.0 40 156223.0 41 156223.0 42 179735.5 43 203248.0 44 203248.0 45 203248.0 46 241027.5 47 278807.0 48 278807.0 49 278807.0 50 280778.0 51 282749.0 52 282749.0 53 282749.0 54 262815.0 55 242881.0 56 242881.0 57 242881.0 58 217234.0 59 191587.0 60 191587.0 61 191587.0 62 162031.5 63 132476.0 64 132476.0 65 132476.0 66 104963.5 67 77451.0 68 77451.0 69 77451.0 70 57445.5 71 37440.0 72 37440.0 73 37440.0 74 26751.5 75 16063.0 76 16063.0 77 16063.0 78 11236.5 79 6410.0 80 6410.0 81 6410.0 82 4384.5 83 2359.0 84 2359.0 85 2359.0 86 1552.5 87 746.0 88 746.0 89 746.0 90 482.5 91 219.0 92 219.0 93 219.0 94 115.5 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18684965557362526 2 0.06325281292557482 3 0.0 4 0.0 5 0.019726838813745325 6 0.3756032419710973 7 0.0011635132812396706 8 0.03173218039744556 9 0.033371676384646914 10 0.002274139595150265 11 0.0030674441050864043 12 0.010365845596498884 13 0.008356140837993997 14 0.00142794811788505 15 0.0 16 0.012534211256990996 17 0.02496264857932384 18 0.06309415202358759 19 0.028558962357701008 20 0.0 21 0.0 22 0.0 23 2.644348366453797E-4 24 2.1154786931630373E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1890825.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.43672895813692 #Duplication Level Percentage of deduplicated Percentage of total 1 78.14980519030229 50.35717815178711 2 13.507545283579173 17.407640686555037 3 4.082868147590646 7.8926000459432695 4 1.6979151451760484 4.37632392014499 5 0.8622358371282973 2.777982846751419 6 0.47113793023294814 1.8215152267390862 7 0.2936139501327234 1.3243665766121158 8 0.19516099889271865 1.0060429111079496 9 0.1420427067985454 0.8237490658612182 >10 0.5574444216716252 5.960771917788723 >50 0.0252048157547476 1.1168609277273545 >100 0.011742478295898062 1.3705609162764 >500 0.0017236245832511648 0.8011998077297324 >1k 0.001395315138822368 1.8263272665207646 >5k 0.0 0.0 >10k+ 1.641547222143962E-4 1.1368797324548348 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 11101 0.587098224320072 No Hit CCCATGTACTCTGCGTTGATACCAC 10395 0.5497600253857443 No Hit GAGTACATGGGAAGCAGTGGTATCA 4335 0.22926500337154418 No Hit CATGTACTCTGCGTTGATACCACTG 3249 0.17182975685216773 No Hit GCGTTGATACCACTGCTTCCCATGT 2764 0.14617957769756587 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2666 0.14099665489931645 No Hit GTATCAACGCAGAGTACATGGGAAG 2648 0.14004468948739307 No Hit GCTTCCCATGTACTCTGCGTTGATA 2624 0.13877540227149524 No Hit ACTCTGCGTTGATACCACTGCTTCC 2223 0.1175677283725358 No Hit TATCAACGCAGAGTACATGGGAAGC 2202 0.1164571020586252 No Hit GTATCAACGCAGAGTACTTTTTTTT 1996 0.10556238678883557 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.288696732907593E-5 7 0.0 0.0 0.0 0.0 5.288696732907593E-5 8 0.0 0.0 0.0 0.0 5.288696732907593E-5 9 0.0 0.0 0.0 0.0 5.288696732907593E-5 10 5.288696732907593E-5 0.0 0.0 0.0 5.288696732907593E-5 11 5.288696732907593E-5 0.0 0.0 0.0 5.288696732907593E-5 12 5.288696732907593E-5 0.0 0.0 0.0 1.586609019872278E-4 13 5.288696732907593E-5 0.0 0.0 0.0 1.586609019872278E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAA 70 1.0621357E-4 12.249583 1 TCTATAC 160 4.3655746E-11 11.304542 3 CTAGACA 220 0.0 11.250453 4 ACTGCGT 85 5.400955E-5 11.160742 8 GCGTGAC 60 0.005938374 11.067735 11 TGCGTAT 60 0.0059430515 11.066564 10 TACCGTG 105 3.524774E-6 10.84215 7 TTACACG 80 3.700275E-4 10.709566 4 ACCGTGC 160 5.5842975E-10 10.672459 8 GTCTTAC 90 9.242215E-5 10.58606 1 TAGACAG 325 0.0 10.251892 5 ACCGTCT 75 0.0026791391 10.119073 8 GTACCGT 180 3.8016879E-10 10.048482 6 CTTACAC 355 0.0 9.921851 3 GGTCTAC 250 0.0 9.908551 1 TATACTG 445 0.0 9.840499 5 TCTAGTA 165 9.778887E-9 9.81434 2 AGGACCG 175 2.4829205E-9 9.791603 5 ATAGACC 175 2.4829205E-9 9.791603 3 CTTATAC 205 4.0017767E-11 9.751799 3 >>END_MODULE