FastQCFastQC Report
Thu 26 May 2016
SRR1513029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121195
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC98900.46624662041915055No Hit
CCCATGTACTCTGCGTTGATACCAC80080.37752304715030915No Hit
GAGTACATGGGAAGCAGTGGTATCA43550.2053087999924571No Hit
GCGTTGATACCACTGCTTCCCATGT29000.13671538920278428No Hit
CATGTACTCTGCGTTGATACCACTG26150.12327956647078653No Hit
GTATCAACGCAGAGTACATGGGAAG25260.11908381831939072No Hit
GCTTCCCATGTACTCTGCGTTGATA22890.10791087099488732No Hit
ACGCAGAGTACATGGGAAGCAGTGG21440.1010751015347481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCCC602.5734653E-514.2474538
TAGGACC4900.012.798464
GTCCTAT2000.011.8905431
GTCCTAC11000.011.7608291
TCCAACG1251.8313585E-811.40065118
CCGCTCC752.0770315E-411.39849959
GTAGGAC8850.011.3791823
TTACACC2100.011.3117714
ACTGCGT855.334453E-511.1744738
GTCCTAG3750.011.1612571
AGGACCT7100.011.1073165
GCGTTTA1201.2795135E-711.08109113
TGCGTTT1658.0035534E-1110.93692212
CTGTAGG10050.010.7902241
TCCTACA12550.010.7501252
TGTAGGA11050.010.7477272
CGTTTAT1252.2472886E-710.63784814
GTCCTAA4650.010.6375621
GGACGTG5250.010.4948486
ACAGCGT1451.4480975E-810.4808858
Produced by FastQC (version 0.11.5)