##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513029_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2121195
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.867123956071932	31.0	30.0	34.0	22.0	34.0
2	28.598947291503137	31.0	28.0	34.0	16.0	34.0
3	28.965741952060043	31.0	30.0	34.0	18.0	34.0
4	31.80111210897631	35.0	32.0	37.0	17.0	37.0
5	32.004297106112354	35.0	32.0	37.0	19.0	37.0
6	32.45805831147066	36.0	33.0	37.0	24.0	37.0
7	32.429619153354594	36.0	33.0	37.0	23.0	37.0
8	32.415177293931016	36.0	33.0	37.0	23.0	37.0
9	33.75275446151815	39.0	34.0	39.0	22.0	39.0
10	33.253533974952795	38.0	33.0	39.0	17.0	39.0
11	33.61443243077605	38.0	34.0	39.0	21.0	39.0
12	33.43836799539882	38.0	34.0	39.0	17.0	39.0
13	33.52664606507181	38.0	34.0	39.0	18.0	39.0
14	33.794020823168076	39.0	33.0	41.0	10.0	41.0
15	34.32833850730366	39.0	33.0	41.0	17.0	41.0
16	33.906611131932706	39.0	33.0	41.0	11.0	41.0
17	33.43797529222915	39.0	32.0	41.0	10.0	41.0
18	32.84420904254441	38.0	32.0	41.0	10.0	41.0
19	33.725365183304696	39.0	33.0	41.0	10.0	41.0
20	33.9749546835628	39.0	34.0	41.0	10.0	41.0
21	33.994978773757246	39.0	34.0	41.0	9.0	41.0
22	33.57834428235028	39.0	33.0	41.0	2.0	41.0
23	31.900185508640178	38.0	30.0	41.0	2.0	41.0
24	32.96557082210735	39.0	32.0	41.0	2.0	41.0
25	32.705755953601624	39.0	32.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.842310147376196
1101	2	1.1674744436057338
1101	3	0.9521753561184383
1101	4	1.070664292026116
1101	5	1.2534421754937242
1101	6	0.9738842430260988
1101	7	0.8965308966487555
1101	8	0.9499573917876916
1101	9	0.9039802614147447
1101	10	1.291316298779563
1101	11	0.9824839923881754
1101	12	0.9605075048288114
1101	13	0.9174804540508887
1101	14	-0.09218493153687746
1101	15	0.544300018072839
1101	16	1.3289366466664845
1101	17	1.9317803648841547
1101	18	-4.218518739315282
1101	19	-0.6057931993471186
1101	20	0.335304165327706
1101	21	0.7638567976549311
1101	22	1.3730493560645627
1101	23	3.3695020187031197
1101	24	2.3221179709848556
1101	25	2.4418746535052662
1102	1	1.030987442971547
1102	2	1.2170950636789968
1102	3	1.083670745329659
1102	4	1.6199408369266663
1102	5	1.4344088805500235
1102	6	1.065820161369082
1102	7	0.9206280546866239
1102	8	0.8555914740415176
1102	9	1.1078315443221598
1102	10	1.274768833543959
1102	11	1.0661306528931433
1102	12	1.1916276272885256
1102	13	1.0704923870980778
1102	14	1.9961735324905803
1102	15	1.5184810727877505
1102	16	1.8038688599257497
1102	17	1.3300044813176584
1102	18	1.7946727580256692
1102	19	1.5060470594441853
1102	20	1.4862099107076432
1102	21	1.415340101389532
1102	22	1.8783458756731193
1102	23	3.2397782184195556
1102	24	2.201936636412732
1102	25	2.516926995676279
1103	1	0.647857969809241
1103	2	0.9038408481898514
1103	3	0.7208545628969105
1103	4	1.1215023415598822
1103	5	1.1391328658586986
1103	6	0.6816236323785674
1103	7	0.5519385386610196
1103	8	0.5444991329735345
1103	9	0.7397105614357926
1103	10	0.9883422001201865
1103	11	0.7027972380821339
1103	12	0.793597395448316
1103	13	0.6583668185272131
1103	14	1.437511263204435
1103	15	1.0157634904175836
1103	16	1.267760215777706
1103	17	1.6018988162349785
1103	18	1.6795758795562676
1103	19	1.1247082846089569
1103	20	0.9723220402897468
1103	21	0.9630004698315986
1103	22	1.4128608767700541
1103	23	2.9489692929321016
1103	24	1.8164183584042561
1103	25	2.034889655361212
1104	1	0.9202211144520582
1104	2	1.26229816259967
1104	3	1.0604283423885157
1104	4	1.6430016041364794
1104	5	1.3885981384097263
1104	6	1.0869791307735213
1104	7	0.9932706962437479
1104	8	0.9747654258046836
1104	9	1.1373065224566616
1104	10	1.4076384580250263
1104	11	1.0776763719709805
1104	12	1.1600646348916328
1104	13	0.9761696687998622
1104	14	1.859021626565145
1104	15	1.3142678408039643
1104	16	1.6258432383337222
1104	17	2.1043757592913295
1104	18	2.8098716058337487
1104	19	1.9731795062477673
1104	20	1.354789289256523
1104	21	1.429510177958349
1104	22	1.918548714189491
1104	23	3.3413848880358152
1104	24	2.3215047297464153
1104	25	2.333357003133898
1105	1	0.5557343380030666
1105	2	0.9355140544662319
1105	3	0.6250318083439979
1105	4	0.7895876366293066
1105	5	1.0030907774356521
1105	6	0.5171476028231794
1105	7	0.3495075187696841
1105	8	0.35957412265170063
1105	9	0.6474163183479007
1105	10	1.0754931444139828
1105	11	0.5554366621501288
1105	12	0.6972748467448113
1105	13	0.6507926760324381
1105	14	1.364014507168001
1105	15	0.779654508276856
1105	16	1.1850184425597377
1105	17	1.6048585255438965
1105	18	1.5370088639733765
1105	19	1.0612552767115062
1105	20	0.7990746527566444
1105	21	0.7706055631509727
1105	22	1.3248868284785402
1105	23	2.783277254224192
1105	24	1.7805764812925347
1105	25	1.7793788522313676
1106	1	-0.7005028652585104
1106	2	-0.26082041137660994
1106	3	-0.5143591243875321
1106	4	-2.8319180763240475
1106	5	-0.7165285201490512
1106	6	-0.6535951548050498
1106	7	-0.6354571057836615
1106	8	-0.5819681065113933
1106	9	-0.6246120527544221
1106	10	-0.5240872198402187
1106	11	-0.6281813903226166
1106	12	-0.537638340299651
1106	13	-0.5402480005727242
1106	14	0.22909377399042086
1106	15	-0.3633111102437141
1106	16	-1.1729344448089165
1106	17	-6.306474705209194
1106	18	-7.452546816171978
1106	19	-3.3649411833267067
1106	20	-1.9160779825240013
1106	21	-1.5430329523124087
1106	22	-1.9128838669388912
1106	23	-5.30740957948321
1106	24	-2.5139832873241588
1106	25	-1.2117034460142193
1107	1	0.4048031533040586
1107	2	0.7639965246685847
1107	3	0.4406001667345052
1107	4	0.698872822618192
1107	5	0.8231756003198853
1107	6	0.5063553885929437
1107	7	0.43394374273876224
1107	8	0.5659113365997968
1107	9	0.4262534669689728
1107	10	0.7558928776524922
1107	11	0.4764271259190025
1107	12	0.5378992443633521
1107	13	0.49260261717751774
1107	14	1.29769223701404
1107	15	0.6887429557696478
1107	16	-5.763147307066951
1107	17	-1.0910051564539813
1107	18	0.6384588967505067
1107	19	0.5335462027685054
1107	20	0.5054423526507179
1107	21	0.5464136206054349
1107	22	0.7267438581021679
1107	23	2.649627624467481
1107	24	1.6341825899944382
1107	25	1.1766800317913138
1108	1	0.4962618740762039
1108	2	0.6702045089774558
1108	3	0.5065050618520424
1108	4	0.9238421099718082
1108	5	0.73836086668571
1108	6	0.245579599573567
1108	7	0.2966922967399128
1108	8	0.44331251569425945
1108	9	0.5074858745165614
1108	10	0.9353807089668109
1108	11	0.49179493575141464
1108	12	0.6125061187235801
1108	13	0.3767144435989067
1108	14	1.0242721023227972
1108	15	0.6288770218378019
1108	16	0.9672412276818392
1108	17	1.0058191446120475
1108	18	0.8711882586430235
1108	19	0.9331902298272752
1108	20	0.6870853417689133
1108	21	0.8501547259895403
1108	22	1.1226302465435225
1108	23	2.7990272308334205
1108	24	1.599551303693822
1108	25	1.7801912536763425
1109	1	0.21106599842291018
1109	2	0.5611278869706666
1109	3	0.410951428229378
1109	4	0.35763687427019164
1109	5	0.1860194437846161
1109	6	0.03431913954971577
1109	7	-0.04160582363941501
1109	8	-0.10509262652197293
1109	9	-0.006174761637041115
1109	10	0.5731789349720131
1109	11	0.15990611505215924
1109	12	0.15393104323543128
1109	13	-0.034351546022655555
1109	14	-1.2877368719776285
1109	15	-0.33553049501129095
1109	16	-0.3752275365771922
1109	17	0.6018064197579704
1109	18	1.4035339993959326
1109	19	0.5575168881255479
1109	20	0.36180816204269917
1109	21	0.29364303047717755
1109	22	0.11726476804719965
1109	23	-4.4091415841780055
1109	24	-1.757150592633927
1109	25	-0.5072074503740822
1110	1	0.41494589207751886
1110	2	0.759241951484583
1110	3	0.4872001353904558
1110	4	0.8343244671650538
1110	5	0.7139577286969896
1110	6	0.3731613137793346
1110	7	0.2620071157648809
1110	8	0.26957692170324776
1110	9	0.3169851691540586
1110	10	0.7225823872632162
1110	11	0.3257971975749854
1110	12	0.466251243852561
1110	13	0.21331007851259898
1110	14	1.0425688996272626
1110	15	0.5049929956752663
1110	16	0.992679820337905
1110	17	1.2833603441946622
1110	18	1.8794044930565477
1110	19	1.0399218510534354
1110	20	0.7055242317265353
1110	21	0.5094241948690623
1110	22	0.7825478765255056
1110	23	2.131405993009661
1110	24	1.154415827457541
1110	25	1.4708143773246434
1111	1	0.4938723056039933
1111	2	0.7974745944569115
1111	3	0.6198837758769251
1111	4	1.0585329345917955
1111	5	0.7910366460437999
1111	6	0.21521218594401148
1111	7	0.29709017739033783
1111	8	0.21399975079840772
1111	9	0.2971596196938364
1111	10	0.47776194332892885
1111	11	0.34062505332450854
1111	12	0.4230715222973771
1111	13	0.283983761462963
1111	14	-0.6238729185033378
1111	15	-0.03556785515183236
1111	16	0.48059864951757447
1111	17	1.0083922097186289
1111	18	1.546509149296206
1111	19	0.6471187940757659
1111	20	0.5498788404319868
1111	21	0.4667735994591098
1111	22	0.6779043126571409
1111	23	0.3656559736438787
1111	24	0.5395842746030226
1111	25	0.1893688268785212
1112	1	0.40992390987472405
1112	2	0.7509905399079138
1112	3	0.5290419595133571
1112	4	1.0039670138037664
1112	5	0.7404943441402381
1112	6	0.13577473721617395
1112	7	0.18167278875205284
1112	8	0.24154726572593432
1112	9	0.3071151740088496
1112	10	0.6449466269036535
1112	11	0.15300407719416853
1112	12	0.34351710435442584
1112	13	0.2597298763361522
1112	14	0.5760661981915547
1112	15	0.40425401320677423
1112	16	0.6802147706840742
1112	17	0.905136392113846
1112	18	1.0265074571241186
1112	19	0.5036796461978099
1112	20	0.35734131935070224
1112	21	0.3772657206099055
1112	22	0.5017327996764749
1112	23	2.088985662410689
1112	24	0.9944818262306256
1112	25	0.9330176645351074
1113	1	0.286265131251632
1113	2	0.6280680161839562
1113	3	0.3469913238575195
1113	4	0.3821974078105015
1113	5	0.5618830304787821
1113	6	0.20570062935529876
1113	7	0.12298520252611667
1113	8	-0.011012027551174697
1113	9	0.1491697913557246
1113	10	0.5410760516345263
1113	11	0.26007261852824115
1113	12	0.09599952508622067
1113	13	-0.023189555441085474
1113	14	0.5836737677680262
1113	15	0.239924203738191
1113	16	0.5606085899268862
1113	17	0.9822624804739775
1113	18	1.5264880259264118
1113	19	0.6749444330835885
1113	20	0.41951373748144505
1113	21	0.36451629817668874
1113	22	0.75289262059583
1113	23	0.4723657499328944
1113	24	0.4334717857557848
1113	25	0.8653706768991469
1114	1	-0.26196206164821945
1114	2	-2.8828049969355263
1114	3	-0.8966556148471874
1114	4	0.2313981122743627
1114	5	0.41814737294338045
1114	6	0.3694456874520853
1114	7	0.3118564412689864
1114	8	0.2821306015991567
1114	9	0.4418035676223653
1114	10	0.5014545027061672
1114	11	0.36543234282250125
1114	12	0.2787086875771507
1114	13	0.2538750573103101
1114	14	1.1052396303480094
1114	15	0.5051282487881892
1114	16	0.8569572400624494
1114	17	1.1284972995586102
1114	18	1.2582691927525076
1114	19	0.7487473484141063
1114	20	0.5478311526409669
1114	21	0.3373351283767789
1114	22	0.5525066854217044
1114	23	2.1576334676781492
1114	24	1.2120451142649245
1114	25	1.174627503870994
1115	1	0.14874749092340878
1115	2	0.40962497375057083
1115	3	0.18248060696173596
1115	4	0.3075051114016034
1115	5	0.3439249242865259
1115	6	-0.14222095863363649
1115	7	-0.2627154717987068
1115	8	-0.24694887655842024
1115	9	-0.35252350127028365
1115	10	-0.05456038408735964
1115	11	-0.05117050300406589
1115	12	-0.28303075529539257
1115	13	-0.2843937454723431
1115	14	0.6228611531811055
1115	15	0.03215667000812772
1115	16	0.43981360672403014
1115	17	0.8286421761187626
1115	18	1.2876579028970099
1115	19	0.590914593405266
1115	20	0.029436928264338746
1115	21	0.14709264636286434
1115	22	0.5222818578734092
1115	23	2.107832737678738
1115	24	0.906002961041537
1115	25	0.5982178055464544
1116	1	0.2800166269626452
1116	2	0.3114114489971662
1116	3	0.23526418106624192
1116	4	1.0406950223572693
1116	5	0.2611265574806154
1116	6	0.3014777294433344
1116	7	0.13793517716764114
1116	8	0.1255565424283418
1116	9	0.22050669902672126
1116	10	0.5907089574963962
1116	11	0.19619944604010442
1116	12	0.34659451618330905
1116	13	0.15279058810326518
1116	14	0.7531128514378267
1116	15	0.10784166903220438
1116	16	0.5604768575806673
1116	17	0.9021598829316702
1116	18	1.3366518350989338
1116	19	0.6993824299615312
1116	20	0.4896595651887097
1116	21	0.32588249433932504
1116	22	0.7012886695036613
1116	23	1.9439379096188425
1116	24	0.8716223514364358
1116	25	1.1680136668721204
1201	1	1.086603004760235
1201	2	1.3812355803271288
1201	3	1.1407037090333176
1201	4	1.7556268239228459
1201	5	1.4883163532535661
1201	6	1.0593063732049401
1201	7	1.0052310055036244
1201	8	1.0481422328225989
1201	9	1.3314367114275285
1201	10	1.5498132493961094
1201	11	1.2104738433197113
1201	12	1.427933850922983
1201	13	1.3271958315296715
1201	14	2.2803003119555427
1201	15	1.5738382571644394
1201	16	1.9435670948366166
1201	17	2.4629139428327633
1201	18	2.9656707943774734
1201	19	2.059724287375637
1201	20	1.8060357520522388
1201	21	1.6836483856014652
1201	22	1.9596289736736523
1201	23	3.5317575776174017
1201	24	2.3703316738351816
1201	25	2.5431766108026395
1202	1	1.1319538232887503
1202	2	1.3660690461194953
1202	3	1.2534873770605408
1202	4	1.8191848574049914
1202	5	1.6012695005294262
1202	6	1.1668863150194113
1202	7	0.9278132463889932
1202	8	0.956806078317797
1202	9	1.248508013567637
1202	10	1.6742579336096597
1202	11	1.25947504594928
1202	12	1.3618817499679992
1202	13	1.2785633241238656
1202	14	2.1381313332081646
1202	15	1.6448551859010792
1202	16	1.9216032070770837
1202	17	2.40452306742673
1202	18	2.9230281276601673
1202	19	2.1987763347844194
1202	20	1.832804934074261
1202	21	1.8260694232906545
1202	22	2.1256552458705826
1202	23	3.6916232238723232
1202	24	2.550358418805885
1202	25	2.6733247187883507
1203	1	1.0030429423599223
1203	2	1.3614848039942196
1203	3	1.0318660652255573
1203	4	1.6599489057606789
1203	5	1.4607242823728512
1203	6	1.1688306721978279
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2211	17	0.2860711544492247
2211	18	-4.914703441737398
2211	19	-1.5428468476166728
2211	20	-0.9138974973277669
2211	21	-0.5749755009224842
2211	22	-0.17783172149079718
2211	23	-4.889833621189737
2211	24	-2.172573368772415
2211	25	-1.44806237032331
2212	1	0.1867770304296883
2212	2	0.41978982334021353
2212	3	0.23056392503682943
2212	4	0.7719925085431072
2212	5	0.626976378441789
2212	6	0.12810714179610727
2212	7	0.03608972376706987
2212	8	0.010813136613926133
2212	9	-0.16419989282188396
2212	10	-3.7870985050080215
2212	11	-1.2115991294392359
2212	12	-0.25890141746716466
2212	13	-0.16905953277066743
2212	14	0.5862838672721793
2212	15	0.11547735358630717
2212	16	0.4248708520550082
2212	17	0.9612762893277278
2212	18	1.4591614881349315
2212	19	0.6875248404810677
2212	20	0.24429052319572975
2212	21	0.28474860017347225
2212	22	0.5861146259786594
2212	23	1.6399575902572003
2212	24	0.992495142987984
2212	25	0.7475002026071778
2213	1	-1.276339832036637
2213	2	-3.534441226495165
2213	3	-1.5087777765495218
2213	4	-2.8218440112114607
2213	5	-0.9982141988554254
2213	6	-0.8081523519931757
2213	7	-0.6973978641557075
2213	8	-0.6518837542869989
2213	9	-0.6740070239203533
2213	10	-0.8408506563100246
2213	11	-0.7392527136954499
2213	12	-0.5352314833157124
2213	13	-0.9471047942941624
2213	14	-6.022631642679929
2213	15	-2.6258484564256612
2213	16	-1.783580958780071
2213	17	-0.6173327536234865
2213	18	0.15717246912864624
2213	19	-0.6151727051639426
2213	20	-0.7304948142780816
2213	21	-0.9773700113949175
2213	22	-0.8494456946588755
2213	23	-5.611191719455459
2213	24	-3.139012545578314
2213	25	-2.179188335629874
2214	1	0.05053590127243268
2214	2	0.2642930146293523
2214	3	-0.023688128680898757
2214	4	0.40154521601841253
2214	5	0.022082641727294572
2214	6	-0.05304678047261291
2214	7	-0.06348462109144037
2214	8	-0.19533950554097856
2214	9	-0.08988478830924151
2214	10	0.34446017540914653
2214	11	-0.1189555629452812
2214	12	0.017682048721226806
2214	13	-0.14378576140314436
2214	14	0.431209669107794
2214	15	0.097622706703973
2214	16	0.5310690939397773
2214	17	0.7566487124932237
2214	18	1.2880216886353537
2214	19	0.3001056606396091
2214	20	0.2030691285497852
2214	21	0.18768051057381285
2214	22	0.5976237140947731
2214	23	1.941668825643145
2214	24	0.8865297434264079
2214	25	0.7960433292955429
2215	1	-2.4734533141595456
2215	2	-4.589071219750856
2215	3	-2.6513123877351035
2215	4	-4.036525807304464
2215	5	-5.092139908369404
2215	6	-2.5641408562266967
2215	7	-1.9603290377531728
2215	8	-2.2067608943460577
2215	9	-1.8111623662985323
2215	10	-3.156902171928312
2215	11	-2.2498453331171397
2215	12	-1.9506324278193077
2215	13	-1.8139198129804939
2215	14	-1.0763591143543678
2215	15	-1.7847487144474243
2215	16	-2.625329278686362
2215	17	-7.129592477684724
2215	18	-3.1210242623955367
2215	19	-2.26921396612887
2215	20	-2.020809870367934
2215	21	-1.9721837230845551
2215	22	-1.9406023758913697
2215	23	-6.315021281482299
2215	24	-3.6952652346080903
2215	25	-3.669014618989795
2216	1	-1.3952105373439458
2216	2	-0.890616077201976
2216	3	-1.1159234944819794
2216	4	-3.6933873415077265
2216	5	-1.5698544098503149
2216	6	-1.3642061100676663
2216	7	-1.3430177292149708
2216	8	-1.2474335382420527
2216	9	-1.335810152056041
2216	10	-0.9891936634288925
2216	11	-1.4144487990427592
2216	12	-2.1909689369520855
2216	13	-1.5543645670810662
2216	14	-7.012656375442155
2216	15	-3.6288241014289397
2216	16	-2.055347717921272
2216	17	-1.227163540391011
2216	18	-0.5374365930239975
2216	19	-0.9833494394240461
2216	20	-1.4596948842932562
2216	21	-1.7185010927272373
2216	22	-1.2632783234452631
2216	23	-0.2199482149783094
2216	24	-0.9669174502007678
2216	25	-0.9755849274334842
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118706.0
3	4.0
4	1021.0
5	5918.0
6	7042.0
7	6129.0
8	5703.0
9	5625.0
10	5198.0
11	5032.0
12	4908.0
13	4811.0
14	4989.0
15	4953.0
16	5049.0
17	5556.0
18	6153.0
19	7160.0
20	9040.0
21	11133.0
22	13728.0
23	16717.0
24	19746.0
25	23071.0
26	25993.0
27	29581.0
28	32892.0
29	39568.0
30	46414.0
31	52913.0
32	67801.0
33	88036.0
34	113750.0
35	149218.0
36	207183.0
37	284465.0
38	685989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.725588885511634	17.18774483798923	11.691621655808053	25.39504462069109
2	14.327159221525172	18.65803036227294	41.37747577229852	25.637334643903376
3	20.32061880854267	22.818046255957427	27.93193181485921	28.92940312064069
4	11.086421278443936	15.257782130985117	38.03283234246517	35.62296424810578
5	13.025578243355024	37.26440409078348	35.855238026969445	13.85477963889206
6	34.61241761216016	34.856575136672575	17.127570184678902	13.403437066488365
7	30.09297548913746	30.22071187217497	20.341582837209433	19.344729801478138
8	26.46146112869176	36.26745470723623	19.306192333391333	17.964891830680678
9	26.603155830259883	15.329510799624444	18.925078622246808	39.14225474786887
10	14.404564337856455	29.104869321871558	34.06874708491968	22.42181925535231
11	35.945004672848725	22.29123305679751	22.048112895270663	19.715649375083107
12	23.561872105425465	24.480921624045514	30.674026229996727	21.283180040532297
13	29.625707380140266	20.628152621538305	24.63861255890316	25.10752743941827
14	21.049946339890116	21.023493508918516	27.474353485266573	30.45220666592479
15	24.92698310308373	28.73598014279908	22.175304681097465	24.161732073019728
16	22.97605709780489	27.5425894699516	26.962595581106726	22.51875785113679
17	22.9309587087696	27.481604644626824	27.333819349216558	22.25361729738702
18	22.569646479458992	26.72622645797635	28.76075900304699	21.943368059517667
19	23.96781322123388	26.800449705682343	26.966637082421208	22.26509999066257
20	23.86285510579967	26.650558823335246	27.16152073884829	22.32506533201679
21	23.469719776364336	26.318499739695326	26.967846714502365	23.24393376943797
22	23.313367191357575	26.847190395501276	26.36940836804732	23.470034045093833
23	21.83429053504424	26.39136919849231	28.499885173520024	23.27445509294343
24	23.175909192093357	27.10635632708655	26.764100542176177	22.953633938643918
25	23.23144772011763	26.1778559127169	26.748463133114647	23.842233234050823
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	138.0
2	240.0
3	342.0
4	342.0
5	342.0
6	670.5
7	999.0
8	999.0
9	999.0
10	1625.0
11	2251.0
12	2251.0
13	2251.0
14	4159.0
15	6067.0
16	6067.0
17	6067.0
18	11326.0
19	16585.0
20	16585.0
21	16585.0
22	27081.0
23	37577.0
24	37577.0
25	37577.0
26	54665.0
27	71753.0
28	71753.0
29	71753.0
30	94790.0
31	117827.0
32	117827.0
33	117827.0
34	141801.5
35	165776.0
36	165776.0
37	165776.0
38	190785.0
39	215794.0
40	215794.0
41	215794.0
42	237605.5
43	259417.0
44	259417.0
45	259417.0
46	287450.5
47	315484.0
48	315484.0
49	315484.0
50	305286.0
51	295088.0
52	295088.0
53	295088.0
54	264548.5
55	234009.0
56	234009.0
57	234009.0
58	201574.0
59	169139.0
60	169139.0
61	169139.0
62	138364.5
63	107590.0
64	107590.0
65	107590.0
66	83597.5
67	59605.0
68	59605.0
69	59605.0
70	43799.0
71	27993.0
72	27993.0
73	27993.0
74	19556.0
75	11119.0
76	11119.0
77	11119.0
78	7818.0
79	4517.0
80	4517.0
81	4517.0
82	2998.5
83	1480.0
84	1480.0
85	1480.0
86	979.5
87	479.0
88	479.0
89	479.0
90	302.5
91	126.0
92	126.0
93	126.0
94	77.5
95	29.0
96	29.0
97	29.0
98	20.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11535950254455624
2	0.047048951180820246
3	0.034555993201945134
4	0.031303109803671986
5	0.03399027435007154
6	0.020413021905105376
7	0.019847303053231787
8	0.023194472926817197
9	0.028851661445553095
10	0.03564028766803618
11	0.020413021905105376
12	0.01965873010260726
13	0.016075844040741187
14	0.020695881331042172
15	0.02135588665822803
16	0.022770183787912002
17	0.025975923948529014
18	0.019470157151982727
19	0.03285883664632436
20	0.0230530432138488
21	0.0284273723066479
22	0.0363945794705343
23	0.028474515544304038
24	0.04011889524536877
25	0.030501674763517738
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2121195.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.63658146086706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.36332155807028	64.56589824797172
2	10.156827830720976	15.364554712046576
3	2.521280909133827	5.7210320660828895
4	0.8743751063855365	2.6453897584593578
5	0.40360891619652656	1.5263799334115422
6	0.20301585132403108	0.921325498591041
7	0.10952451079141269	0.579884170770541
8	0.07934517898150316	0.4801118474849232
9	0.057080128839628416	0.38856112332978077
>10	0.20768117611938056	2.7225254778933308
>50	0.012911033821458315	0.6682886168810508
>100	0.008257226480111114	1.1984009689312638
>500	0.0016371568526437326	0.9067136559021092
>1k	0.0010074811400883408	1.4585144972341624
>5k	1.259351425110426E-4	0.8524194250096996
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	9890	0.46624662041915055	No Hit
CCCATGTACTCTGCGTTGATACCAC	8008	0.37752304715030915	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4355	0.2053087999924571	No Hit
GCGTTGATACCACTGCTTCCCATGT	2900	0.13671538920278428	No Hit
CATGTACTCTGCGTTGATACCACTG	2615	0.12327956647078653	No Hit
GTATCAACGCAGAGTACATGGGAAG	2526	0.11908381831939072	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2289	0.10791087099488732	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2144	0.1010751015347481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.714323765613251E-5
2	0.0	0.0	0.0	0.0	4.714323765613251E-5
3	0.0	0.0	0.0	0.0	4.714323765613251E-5
4	0.0	0.0	0.0	0.0	4.714323765613251E-5
5	0.0	0.0	0.0	0.0	4.714323765613251E-5
6	0.0	0.0	0.0	0.0	4.714323765613251E-5
7	0.0	0.0	0.0	0.0	4.714323765613251E-5
8	0.0	0.0	0.0	0.0	4.714323765613251E-5
9	0.0	0.0	0.0	0.0	4.714323765613251E-5
10	0.0	0.0	0.0	9.428647531226502E-5	4.714323765613251E-5
11	0.0	0.0	0.0	1.4142971296839753E-4	4.714323765613251E-5
12	0.0	0.0	0.0	1.8857295062453005E-4	1.8857295062453005E-4
13	0.0	0.0	0.0	1.8857295062453005E-4	1.8857295062453005E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCCC	60	2.5734653E-5	14.247453	8
TAGGACC	490	0.0	12.79846	4
GTCCTAT	200	0.0	11.890543	1
GTCCTAC	1100	0.0	11.760829	1
TCCAACG	125	1.8313585E-8	11.400651	18
CCGCTCC	75	2.0770315E-4	11.3984995	9
GTAGGAC	885	0.0	11.379182	3
TTACACC	210	0.0	11.311771	4
ACTGCGT	85	5.334453E-5	11.174473	8
GTCCTAG	375	0.0	11.161257	1
AGGACCT	710	0.0	11.107316	5
GCGTTTA	120	1.2795135E-7	11.081091	13
TGCGTTT	165	8.0035534E-11	10.936922	12
CTGTAGG	1005	0.0	10.790224	1
TCCTACA	1255	0.0	10.750125	2
TGTAGGA	1105	0.0	10.747727	2
CGTTTAT	125	2.2472886E-7	10.637848	14
GTCCTAA	465	0.0	10.637562	1
GGACGTG	525	0.0	10.494848	6
ACAGCGT	145	1.4480975E-8	10.480885	8
>>END_MODULE