FastQCFastQC Report
Thu 26 May 2016
SRR1513029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121195
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC116140.547521562138323No Hit
CCCATGTACTCTGCGTTGATACCAC107080.5048097888218669No Hit
GAGTACATGGGAAGCAGTGGTATCA46590.21964034423992135No Hit
CATGTACTCTGCGTTGATACCACTG33130.156185546354767No Hit
ACGCAGAGTACATGGGAAGCAGTGG29770.14034541850230647No Hit
GTATCAACGCAGAGTACATGGGAAG28830.13591395416263002No Hit
GCGTTGATACCACTGCTTCCCATGT28660.13511251912247577No Hit
GTATCAACGCAGAGTACTTTTTTTT27450.12940818736608373No Hit
GCTTCCCATGTACTCTGCGTTGATA25570.12054525868673083No Hit
TATCAACGCAGAGTACATGGGAAGC25180.11870667241814165No Hit
ACTCTGCGTTGATACCACTGCTTCC23330.10998517345175715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG707.3758565E-613.5516397
TAGGACC6100.013.1089484
GTAGGAC10150.012.1925833
GGACCGA657.889565E-411.7161456
AGACCCG700.001466365210.8795365
CTAGGAC3150.010.8795353
TGTAGGA12550.010.8552472
AGGACCT9500.010.7220675
TATACCG803.700753E-410.7095425
GGACTGT2950.010.3260956
TAGGACA4650.010.2361214
TCTAGAC2450.010.1024263
GTAAGAC1803.8016879E-1010.0484593
CCCTATA2002.3646862E-1110.0031472
CTGTAGG12350.09.9529631
GTCCTAC10250.09.9469411
GTTCTAG2400.09.9256371
TAGGACG6800.09.7995814
TGGACTG3600.09.7840265
GTAGAAC3600.09.7840263
Produced by FastQC (version 0.11.5)