FastQCFastQC Report
Thu 26 May 2016
SRR1513028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202246
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC105420.47869311602790965No Hit
CCCATGTACTCTGCGTTGATACCAC86280.3917818445350792No Hit
GAGTACATGGGAAGCAGTGGTATCA42660.1937113292520454No Hit
GCGTTGATACCACTGCTTCCCATGT30530.13863119742299454No Hit
GTATCAACGCAGAGTACATGGGAAG29380.13340925582337307No Hit
CATGTACTCTGCGTTGATACCACTG27730.12591690483261178No Hit
GTATCAACGCAGAGTACTTTTTTTT24860.11288475492746951No Hit
ACGCAGAGTACATGGGAAGCAGTGG22280.10116944246918828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC707.2716666E-613.5703746
GCGTTTA2000.012.82342113
CGTTTAT2050.012.51065414
TGCGTTT2250.012.24293712
TGGCCCG658.021805E-411.6929935
TAGGACC3950.011.5449794
GTCCTAC7750.011.41492751
GTAGGAC8150.010.7235793
GTCTAAG3050.010.60403251
TGTAGGA10100.010.4412052
CGCTAGG750.002647034610.1339262
CTAACAC2350.010.1060563
CACCGTG1901.0004442E-109.9987697
CTAGACA2950.09.9836364
ACACCGT1252.5733698E-69.8792326
TAAGACC2700.09.8524294
CTAGGAC3700.09.7564423
GTGTAGG4100.09.7444511
GTCCTAA4400.09.7286321
GTATTAC2251.8189894E-129.7238281
Produced by FastQC (version 0.11.5)