##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202246 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.305510374408673 31.0 30.0 34.0 25.0 34.0 2 29.034857595382167 31.0 30.0 34.0 16.0 34.0 3 29.401040574032148 31.0 30.0 34.0 23.0 34.0 4 32.27218348903801 35.0 33.0 37.0 19.0 37.0 5 32.48296920507518 35.0 33.0 37.0 22.0 37.0 6 32.94668306810411 37.0 33.0 37.0 26.0 37.0 7 32.93376580091416 37.0 35.0 37.0 26.0 37.0 8 32.9175346441769 37.0 35.0 37.0 26.0 37.0 9 34.28623550684165 39.0 35.0 39.0 26.0 39.0 10 33.78984454960981 38.0 34.0 39.0 22.0 39.0 11 34.16315979232111 39.0 34.0 39.0 26.0 39.0 12 34.00199886842796 39.0 34.0 39.0 25.0 39.0 13 34.08348613188536 39.0 35.0 39.0 25.0 39.0 14 34.37329707943618 39.0 33.0 41.0 17.0 41.0 15 34.91873205808979 40.0 34.0 41.0 24.0 41.0 16 34.50192485308181 39.0 34.0 41.0 17.0 41.0 17 34.036395570703725 39.0 33.0 41.0 16.0 41.0 18 33.43092143202894 39.0 32.0 41.0 10.0 41.0 19 34.33507155876319 39.0 34.0 41.0 18.0 41.0 20 34.60270151472633 39.0 34.0 41.0 18.0 41.0 21 34.63003497338626 39.0 34.0 41.0 18.0 41.0 22 34.21215795147318 39.0 34.0 41.0 11.0 41.0 23 32.51728598894038 39.0 31.0 41.0 2.0 41.0 24 33.611888499286636 39.0 33.0 41.0 2.0 41.0 25 33.3420748635711 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6134001652566674 1101 2 1.0138172731155208 1101 3 0.7709180384448722 1101 4 0.7364772703605453 1101 5 0.9569636569443176 1101 6 0.6353427897663195 1101 7 0.7178797690747984 1101 8 0.7305799667218125 1101 9 0.7336909116823023 1101 10 1.061800661759257 1101 11 0.794264752452527 1101 12 0.8412370750694436 1101 13 0.798337326740338 1101 14 -0.10748758559327598 1101 15 0.38129574659955523 1101 16 1.0401665067649546 1101 17 1.730565632374791 1101 18 -4.591394861210016 1101 19 -1.0553608002852357 1101 20 0.02075179019291795 1101 21 0.3681099197256614 1101 22 1.038694443055192 1101 23 2.8439144208796705 1101 24 1.8875209670713105 1101 25 1.983330942410113 1102 1 0.7283492810710506 1102 2 1.0586771735639573 1102 3 0.7833781871045034 1102 4 1.2956582802453696 1102 5 1.1098283684878254 1102 6 0.6802791656157652 1102 7 0.6796000471639303 1102 8 0.6174405313221598 1102 9 0.7533589781722796 1102 10 1.1478869463100665 1102 11 0.6881230388159523 1102 12 0.7942025667688739 1102 13 0.7317627747980495 1102 14 1.6055070005893413 1102 15 0.932141471945144 1102 16 1.3403666426222998 1102 17 0.9296001193085317 1102 18 1.4128340771173313 1102 19 1.2013808312671443 1102 20 1.04168717604648 1102 21 0.9807226630736849 1102 22 1.3854454849101145 1102 23 2.9752216160664418 1102 24 1.8184009149362765 1102 25 2.062589816539081 1103 1 0.7128609201997484 1103 2 0.9425170933671687 1103 3 0.7107023404221025 1103 4 1.2499583967835548 1103 5 1.0809900200359905 1103 6 0.719225354176146 1103 7 0.7259684449286041 1103 8 0.67815336396567 1103 9 0.7486699410412001 1103 10 1.061800661759257 1103 11 0.7852773348371898 1103 12 0.8972586448717195 1103 13 0.6635260625102646 1103 14 1.5177370998471105 1103 15 0.9873472744605536 1103 16 1.3652114438530347 1103 17 1.706599185400556 1103 18 1.7669035210548145 1103 19 1.2022785046878042 1103 20 1.1067314187129895 1103 21 1.1838678586112223 1103 22 1.4796770673811324 1103 23 2.9682403645585183 1103 24 1.92287147635831 1103 25 2.068711409755828 1104 1 0.14837364833830335 1104 2 0.6069603108254178 1104 3 0.3618787597660855 1104 4 0.9430173709564613 1104 5 0.7721206604145507 1104 6 0.17937403911761152 1104 7 0.21159389946696194 1104 8 0.19501965344053218 1104 9 0.28294171182062655 1104 10 0.5712220587149801 1104 11 0.2325837975276528 1104 12 0.35097535146176284 1104 13 0.17301887266488336 1104 14 0.929163885882339 1104 15 0.4692023782332413 1104 16 0.8801380443913729 1104 17 1.2873636987360157 1104 18 1.9511312108174224 1104 19 1.07888598918138 1104 20 0.4615710069258583 1104 21 0.49016909314583046 1104 22 0.912139786586387 1104 23 2.332317449650944 1104 24 1.4247941325815532 1104 25 1.4463381956846035 1105 1 0.6085241717423742 1105 2 1.0328101144173196 1105 3 0.7815407259241063 1105 4 0.9913644449038657 1105 5 1.0615999859888632 1105 6 0.6059524788556274 1105 7 0.44830995118004324 1105 8 0.5173302343565993 1105 9 0.7292034145574249 1105 10 1.3325885019110046 1105 11 0.8381547282873072 1105 12 0.7648887494551602 1105 13 0.6562133145753819 1105 14 1.4579922473798987 1105 15 0.8819390994600553 1105 16 1.2777651482876777 1105 17 1.8253802260668408 1105 18 1.5952028474954218 1105 19 1.300578207378912 1105 20 1.0510529112774307 1105 21 0.8835762999686807 1105 22 1.417512853205153 1105 23 2.94957585506139 1105 24 1.9692374289535834 1105 25 1.7943261178848218 1106 1 -0.7945807271076326 1106 2 -0.3432645862788952 1106 3 -0.6417063530206875 1106 4 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1107 16 -6.19234663373048 1107 17 -1.5508932115411014 1107 18 0.3372974476916326 1107 19 0.21767747091642065 1107 20 -0.01884245716107813 1107 21 0.12353215367102877 1107 22 0.20561148805516893 1107 23 2.0591944282862116 1107 24 1.0748961345482115 1107 25 0.7813869002207809 1108 1 0.3372484747359472 1108 2 0.6519302790792416 1108 3 0.42515525895842643 1108 4 0.964603915449878 1108 5 0.7907425068020117 1108 6 0.3207441589043327 1108 7 0.1913795093912114 1108 8 0.24892393749037467 1108 9 0.3808051050231782 1108 10 0.7926884971632902 1108 11 0.38434370967905807 1108 12 0.40980925315977146 1108 13 0.32402199283950495 1108 14 0.9133956704822808 1108 15 0.6816991653136526 1108 16 0.83458272712069 1108 17 0.8984878777074599 1108 18 0.7087742950178182 1108 19 0.6194399706801974 1108 20 0.42516987123185146 1108 21 0.5053646105362546 1108 22 0.914092927604635 1108 23 2.645017979021432 1108 24 1.4761525104886033 1108 25 1.5928547452087614 1109 1 0.33905774991364623 1109 2 0.6484571477482461 1109 3 0.4848981263452572 1109 4 0.4105759120979897 1109 5 0.25750251567222193 1109 6 0.0704911436184048 1109 7 0.0813568917244254 1109 8 0.08680797669893536 1109 9 0.1624016193878859 1109 10 0.6544404268150288 1109 11 0.3128120570350177 1109 12 0.3629896812098252 1109 13 0.2758162770773964 1109 14 -0.872524037491452 1109 15 -0.0011877097385948332 1109 16 -0.368870922896825 1109 17 0.8289718847234937 1109 18 1.5468215420387992 1109 19 0.6934368384718539 1109 20 0.5561836041762049 1109 21 0.3826088474870559 1109 22 0.30109595549814827 1109 23 -4.603571525524554 1109 24 -1.5521753572540007 1109 25 -0.18269254498888898 1110 1 0.7420771397944748 1110 2 1.0775917298832418 1110 3 0.8901313699965279 1110 4 1.3679000410714792 1110 5 1.0291980970734613 1110 6 0.6318564476237398 1110 7 0.6441025966844904 1110 8 0.5933474016654046 1110 9 0.7255915457586255 1110 10 1.0873184620187502 1110 11 0.7157690349462413 1110 12 0.8222214236002046 1110 13 0.5710290259872934 1110 14 1.5013061176107314 1110 15 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1.0348343194887804 2209 21 0.8122697481657895 2209 22 0.30214907260196355 2209 23 2.5379796944771797 2209 24 1.5931450657964277 2209 25 1.7794899803376794 2210 1 0.44678642189095186 2210 2 0.3761930920602232 2210 3 0.40323739603581643 2210 4 1.0603939000242804 2210 5 0.9547307274417989 2210 6 0.5489686657554032 2210 7 0.4257098321819299 2210 8 0.5135369980334943 2210 9 0.5073718764896995 2210 10 0.9548431043379111 2210 11 0.6058918975597152 2210 12 0.6819211946261277 2210 13 0.5775664754369458 2210 14 0.7491274886633192 2210 15 0.720190798305623 2210 16 0.9943657277686668 2210 17 1.6585978381988866 2210 18 2.1647645000456492 2210 19 1.2613642468555426 2210 20 1.0419622345803319 2210 21 0.9915164728796597 2210 22 0.36316789525252346 2210 23 2.54787127919289 2210 24 1.6214106370849848 2210 25 1.952753160514149 2211 1 -0.6084501668197646 2211 2 -0.10027078428297287 2211 3 -0.28463004280534676 2211 4 0.4862780279769936 2211 5 -2.76276597135848 2211 6 -0.874880930098108 2211 7 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1.6308500446597947 2212 19 0.8084174645391329 2212 20 0.4249119885435988 2212 21 0.33554445828204393 2212 22 0.5869981842231269 2212 23 1.9631540128660347 2212 24 1.030874840838635 2212 25 1.0142216379950355 2213 1 -1.3387208085976035 2213 2 -3.6920311035391506 2213 3 -1.6460557835212661 2213 4 -3.24286429841764 2213 5 -1.1564833961604037 2213 6 -0.8975314329522135 2213 7 -0.7542429127410628 2213 8 -0.6542081438035794 2213 9 -0.648731912595423 2213 10 -0.7408061446910637 2213 11 -0.8324637639594528 2213 12 -0.7152361423229721 2213 13 -1.0828136331804643 2213 14 -6.321968986424196 2213 15 -2.737252681929725 2213 16 -1.9853054035385398 2213 17 -0.8143907003395654 2213 18 -0.05501820035053839 2213 19 -0.5768702912422299 2213 20 -0.7130776882275711 2213 21 -0.9473704324443304 2213 22 -0.8232608499332486 2213 23 -5.432259961655269 2213 24 -3.0565544595367733 2213 25 -2.3072593920049904 2214 1 0.038728886407259466 2214 2 0.1440127492390424 2214 3 0.01549074999882194 2214 4 0.3703710113984968 2214 5 -0.05780148210450875 2214 6 -0.10365066306051318 2214 7 -0.2793281700371466 2214 8 -0.29102069076241577 2214 9 -0.15019113288435193 2214 10 0.29466734361876235 2214 11 -0.19698871854869537 2214 12 -0.16591236156310174 2214 13 -0.04019955286247523 2214 14 0.47839100889165564 2214 15 0.0020214409549126344 2214 16 0.42862275198470456 2214 17 0.701473950989012 2214 18 1.1283980165025795 2214 19 0.24769259079428707 2214 20 0.21421075452781935 2214 21 -0.009684006728605254 2214 22 0.45108837618357 2214 23 1.9177028886452874 2214 24 0.8468620205787687 2214 25 0.6505877987641924 2215 1 -2.293455687062977 2215 2 -4.1942194765439105 2215 3 -2.285507844719863 2215 4 -3.9492853677957704 2215 5 -4.767914471812382 2215 6 -2.1221582280767883 2215 7 -1.4713226057097337 2215 8 -1.9044313778061621 2215 9 -1.5200211218561037 2215 10 -3.0508505259403123 2215 11 -1.836369991287718 2215 12 -1.5792922598251096 2215 13 -1.5979321266874607 2215 14 -0.8342533959649927 2215 15 -1.520486919852054 2215 16 -2.25140995488848 2215 17 -6.73816253964543 2215 18 -2.836433094071591 2215 19 -2.125320716120285 2215 20 -1.8860611225194148 2215 21 -1.9092841793257946 2215 22 -1.5980012636319074 2215 23 -6.194659715058322 2215 24 -3.4436091851209447 2215 25 -3.096678668753057 2216 1 -0.8603413090081276 2216 2 -0.3504697661254319 2216 3 -0.6700696746472126 2216 4 -3.270950850817748 2216 5 -1.1676167250414515 2216 6 -0.7960138588707935 2216 7 -0.8382350400554301 2216 8 -0.6898850808417336 2216 9 -0.7094434399514356 2216 10 -0.38599976011587245 2216 11 -0.904741460766779 2216 12 -1.6072316167072813 2216 13 -0.9755266062192689 2216 14 -6.679494118912384 2216 15 -3.0143520451221697 2216 16 -1.4900605999431846 2216 17 -0.599994213977304 2216 18 0.1398063338292559 2216 19 -0.35267278268795366 2216 20 -1.000570960695562 2216 21 -1.1503693599676978 2216 22 -0.6166481868519824 2216 23 0.5895846195877823 2216 24 -0.07330866596299046 2216 25 0.0015786011673029066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100182.0 3 4.0 4 882.0 5 5004.0 6 6121.0 7 5548.0 8 5186.0 9 4990.0 10 4751.0 11 4594.0 12 4631.0 13 4490.0 14 4561.0 15 4599.0 16 4942.0 17 5112.0 18 5918.0 19 7000.0 20 8505.0 21 10833.0 22 13214.0 23 16211.0 24 19554.0 25 22692.0 26 26454.0 27 30313.0 28 33362.0 29 41075.0 30 47824.0 31 54449.0 32 70171.0 33 91379.0 34 119063.0 35 156645.0 36 219343.0 37 301883.0 38 740761.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.955620407292955 17.59494114015295 12.252477441946 25.196961010608092 2 14.100439395095785 19.179923024084957 41.58357592739986 25.136061653419397 3 19.65214887036279 23.74870483620181 28.4437729705618 28.155373322873604 4 10.881045302751161 15.931955027925856 38.7266391223614 34.46036054696159 5 12.834299658228055 37.54581034109967 36.00629216504623 13.613597835626045 6 33.42977032704858 35.51839577288043 17.771778599435095 13.280055300635892 7 29.36200574895619 30.778897724625086 21.01870704349323 18.84038948292549 8 26.122733175183026 35.928369993591325 19.92312314603741 18.025773685188238 9 26.659377955266635 15.442786946453863 19.368062362541778 38.52977273573773 10 14.370340558288106 29.104396932195414 34.359220584307394 22.16604192520909 11 35.027425674537156 22.80252833062869 22.68389644871919 19.486149546114966 12 23.378929650747164 24.826027597595772 30.885381231418496 20.909661520238572 13 29.02949359235788 21.09217394779604 25.13295323770086 24.745379222145218 14 21.08000921081735 21.35092896179827 27.949743818465144 29.619318008919233 15 24.740469106319896 28.764202835820964 22.752106155027725 23.743221902831415 16 22.682205229662976 27.863406711394767 27.140254016354277 22.31413404258798 17 22.590941112827526 27.735478059989543 27.51936417642124 22.15421665076169 18 22.31052391344335 26.87543572403425 29.008377255829902 21.805663106692492 19 23.56920863021775 27.073587968788182 27.50929604437754 21.84790735661652 20 23.676123532735375 26.837067087671617 27.492873740300748 21.99393563929226 21 23.33163305397577 26.456271101140583 27.437512462532915 22.774583382350738 22 23.124742209971455 26.99154721259705 26.66521002051418 23.218500556917313 23 21.675589718572986 26.48354538356405 28.79904215159786 23.041822746265105 24 22.84359793436034 27.444452318393424 27.018986513742465 22.692963233503775 25 23.065927307446678 26.395496619193963 26.94815370653168 23.590422366827674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 187.0 2 301.5 3 416.0 4 416.0 5 416.0 6 840.0 7 1264.0 8 1264.0 9 1264.0 10 2123.5 11 2983.0 12 2983.0 13 2983.0 14 5440.5 15 7898.0 16 7898.0 17 7898.0 18 14490.0 19 21082.0 20 21082.0 21 21082.0 22 33391.5 23 45701.0 24 45701.0 25 45701.0 26 64910.0 27 84119.0 28 84119.0 29 84119.0 30 109516.5 31 134914.0 32 134914.0 33 134914.0 34 159458.0 35 184002.0 36 184002.0 37 184002.0 38 207088.0 39 230174.0 40 230174.0 41 230174.0 42 250840.5 43 271507.0 44 271507.0 45 271507.0 46 297251.5 47 322996.0 48 322996.0 49 322996.0 50 310424.0 51 297852.0 52 297852.0 53 297852.0 54 263515.0 55 229178.0 56 229178.0 57 229178.0 58 196681.5 59 164185.0 60 164185.0 61 164185.0 62 134026.0 63 103867.0 64 103867.0 65 103867.0 66 80090.5 67 56314.0 68 56314.0 69 56314.0 70 41297.0 71 26280.0 72 26280.0 73 26280.0 74 18546.0 75 10812.0 76 10812.0 77 10812.0 78 7536.5 79 4261.0 80 4261.0 81 4261.0 82 2901.5 83 1542.0 84 1542.0 85 1542.0 86 1025.5 87 509.0 88 509.0 89 509.0 90 334.5 91 160.0 92 160.0 93 160.0 94 96.0 95 32.0 96 32.0 97 32.0 98 21.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12255669893372492 2 0.047133698960061685 3 0.035736243816540024 4 0.03187654785160241 5 0.03532757012613486 6 0.021023990961954297 7 0.020660725459371934 8 0.024656645987777934 9 0.030332669465627363 10 0.036417366633881953 11 0.02165970559147343 12 0.02088776639848591 13 0.015620416611041637 14 0.022113787469701388 15 0.022022971094055797 16 0.022613277535752135 17 0.02856175014053834 18 0.020479092708080748 19 0.03505512099919809 20 0.02406633954608159 21 0.029379097521348662 22 0.03823369414679377 23 0.02928828114570307 24 0.040594919913579136 25 0.03178573147595682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2202246.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.77832987952333 #Duplication Level Percentage of deduplicated Percentage of total 1 83.93388921568085 61.08568277410187 2 10.854826424359052 15.799922765939394 3 2.81595043599683 6.1482050926609455 4 1.0438913765538722 3.0389068384490967 5 0.4482379519974433 1.6311004767495938 6 0.24436224548078916 1.0670565667021161 7 0.15276762809118039 0.7782720982492584 8 0.10179655134999786 0.5926866395798386 9 0.06667659889855819 0.43673503588955376 >10 0.3082045899185563 3.9147305247399165 >50 0.016429127511796725 0.8334936873451129 >100 0.009808097682090393 1.2703247772943616 >500 0.0020222441465467915 1.02903305980341 >1k 0.0010111220732729664 1.492176123726253 >5k 6.31951295795604E-5 0.3968220809860101 >10k+ 6.31951295795604E-5 0.4848514577833238 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 10542 0.47869311602790965 No Hit CCCATGTACTCTGCGTTGATACCAC 8628 0.3917818445350792 No Hit GAGTACATGGGAAGCAGTGGTATCA 4266 0.1937113292520454 No Hit GCGTTGATACCACTGCTTCCCATGT 3053 0.13863119742299454 No Hit GTATCAACGCAGAGTACATGGGAAG 2938 0.13340925582337307 No Hit CATGTACTCTGCGTTGATACCACTG 2773 0.12591690483261178 No Hit GTATCAACGCAGAGTACTTTTTTTT 2486 0.11288475492746951 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2228 0.10116944246918828 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.540818782279545E-5 0.0 6 0.0 0.0 0.0 4.540818782279545E-5 0.0 7 0.0 0.0 0.0 4.540818782279545E-5 0.0 8 0.0 0.0 0.0 9.08163756455909E-5 0.0 9 0.0 0.0 0.0 1.3622456346838637E-4 0.0 10 0.0 0.0 0.0 1.3622456346838637E-4 0.0 11 0.0 0.0 0.0 2.7244912693677275E-4 0.0 12 0.0 0.0 0.0 2.7244912693677275E-4 0.0 13 0.0 0.0 0.0 2.7244912693677275E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGC 70 7.2716666E-6 13.570374 6 GCGTTTA 200 0.0 12.823421 13 CGTTTAT 205 0.0 12.510654 14 TGCGTTT 225 0.0 12.242937 12 TGGCCCG 65 8.021805E-4 11.692993 5 TAGGACC 395 0.0 11.544979 4 GTCCTAC 775 0.0 11.4149275 1 GTAGGAC 815 0.0 10.723579 3 GTCTAAG 305 0.0 10.6040325 1 TGTAGGA 1010 0.0 10.441205 2 CGCTAGG 75 0.0026470346 10.133926 2 CTAACAC 235 0.0 10.106056 3 CACCGTG 190 1.0004442E-10 9.998769 7 CTAGACA 295 0.0 9.983636 4 ACACCGT 125 2.5733698E-6 9.879232 6 TAAGACC 270 0.0 9.852429 4 CTAGGAC 370 0.0 9.756442 3 GTGTAGG 410 0.0 9.744451 1 GTCCTAA 440 0.0 9.728632 1 GTATTAC 225 1.8189894E-12 9.723828 1 >>END_MODULE