FastQCFastQC Report
Thu 26 May 2016
SRR1513028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202246
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC124490.5652865302059806No Hit
CCCATGTACTCTGCGTTGATACCAC113890.5171538511138174No Hit
GAGTACATGGGAAGCAGTGGTATCA47470.21555266759481No Hit
GTATCAACGCAGAGTACTTTTTTTT38740.1759113196255096No Hit
CATGTACTCTGCGTTGATACCACTG32760.1487572233074779No Hit
GTATCAACGCAGAGTACATGGGAAG32190.14616895660157855No Hit
GCGTTGATACCACTGCTTCCCATGT30720.13949395299162765No Hit
TATCAACGCAGAGTACATGGGAAGC28230.12818731422375157No Hit
ACGCAGAGTACATGGGAAGCAGTGG27900.12668884402559932No Hit
TATCAACGCAGAGTACTTTTTTTTT25770.11701690001934388No Hit
GCTTCCCATGTACTCTGCGTTGATA24910.11311179586658347No Hit
ACTCTGCGTTGATACCACTGCTTCC23800.1080714870182532No Hit
GGTATCAACGCAGAGTACTTTTTTT22160.10062454421531472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3900.013.6733364
TGGCGAG6150.011.88022918
CCGTCCA1201.0233634E-811.858259
TCCAACG1052.7637907E-711.74798218
GACCGCT658.1213383E-411.6758167
GTCTAGG2550.011.5858831
CCTATAC2650.011.498873
GTAGGAC7100.011.4001783
TCGCTCA600.005939041311.0676999
GGCGAGA4200.011.06719719
GGACCGC803.6912182E-410.712576
TAGGACG5500.010.561324
TATGGCG6900.010.45423216
GTCCTAC8300.010.448911
GTCCTAG3700.010.3030531
TGTAGGA9150.010.100422
CCGTTCA856.6814024E-410.0446359
AGCGTGC951.6681169E-49.9858958
GGCGAGG2850.09.9854419
AGGACCG1159.98289E-69.9365235
Produced by FastQC (version 0.11.5)