FastQCFastQC Report
Thu 26 May 2016
SRR1513027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160373
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70680.32716572554831963No Hit
CCCATGTACTCTGCGTTGATACCAC61550.2849045049165121No Hit
GAGTACATGGGAAGCAGTGGTATCA30520.14127190073195695No Hit
GTATCAACGCAGAGTACTTTTTTTT29970.1387260440673902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGC1551.8189894E-1212.2559448
TAGGACG3250.011.1093714
CACCGTA600.005884489511.0814167
TCGAACT2150.011.04562319
TACACCG700.001490794410.8580295
TGCGCCA1951.8189894E-1210.71659112
GTATAAG2600.010.6078281
ACACCGT1355.696893E-810.5547066
GCGTGCG1451.4479156E-810.4809459
ATTACAC2151.8189894E-1210.1643273
GTATAAT3850.010.1280281
TTAGACA2550.010.0603444
ACCGTGC856.60566E-410.0570838
AGGCGTG2002.5465852E-119.9732747
GCGTTTA1054.1108986E-59.9513513
TACTCCG1151.0257363E-59.9138545
TGTACCG1252.5680492E-69.8808075
CGTGCGC1651.0253643E-89.78618510
GTAGACC5150.09.7781763
GTCTTAC2151.0913936E-119.7316391
Produced by FastQC (version 0.11.5)