##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513027_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2160373
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.265013495354737	31.0	30.0	34.0	25.0	34.0
2	28.995510960375825	31.0	30.0	34.0	16.0	34.0
3	29.35770258191525	31.0	30.0	34.0	23.0	34.0
4	32.21387371532601	35.0	33.0	37.0	19.0	37.0
5	32.461444852347256	35.0	33.0	37.0	22.0	37.0
6	32.91502439625009	37.0	33.0	37.0	26.0	37.0
7	32.91250492391823	37.0	35.0	37.0	26.0	37.0
8	32.880223461411525	37.0	35.0	37.0	26.0	37.0
9	34.26559580220638	39.0	35.0	39.0	26.0	39.0
10	33.78584762908998	39.0	34.0	39.0	21.0	39.0
11	34.14077569012388	39.0	35.0	39.0	25.0	39.0
12	33.976455917566085	39.0	35.0	39.0	23.0	39.0
13	34.055201115733254	39.0	35.0	39.0	25.0	39.0
14	34.37630538800476	40.0	34.0	41.0	17.0	41.0
15	34.909312419660864	40.0	34.0	41.0	23.0	41.0
16	34.494928422082666	39.0	34.0	41.0	17.0	41.0
17	34.03642287697541	39.0	33.0	41.0	14.0	41.0
18	33.45004219178818	39.0	32.0	41.0	10.0	41.0
19	34.342414018319985	39.0	34.0	41.0	18.0	41.0
20	34.59856469230082	39.0	34.0	41.0	18.0	41.0
21	34.62385152934239	40.0	34.0	41.0	18.0	41.0
22	34.20947493789267	40.0	34.0	41.0	10.0	41.0
23	32.52723580603905	39.0	31.0	41.0	2.0	41.0
24	33.62959359332856	39.0	33.0	41.0	2.0	41.0
25	33.36184538503305	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8661984031726746
1101	2	1.2717662575226747
1101	3	1.0155412164596171
1101	4	0.9968232930227146
1101	5	1.1565337900631292
1101	6	0.9030780100367934
1101	7	0.9663750940713598
1101	8	0.9130709473698388
1101	9	1.0184911695327301
1101	10	1.3593330060245208
1101	11	1.048315713274313
1101	12	1.1215257781295591
1101	13	0.9845412190226597
1101	14	0.25773960066734247
1101	15	0.8741228288035643
1101	16	1.430235245845921
1101	17	1.9634562701625882
1101	18	-4.113499331763222
1101	19	-0.5284390894384288
1101	20	0.4588393260090484
1101	21	0.8195172044472798
1101	22	1.446806171936231
1101	23	3.2484579454876368
1101	24	2.1675014484853037
1101	25	2.29420937804381
1102	1	1.118327091866675
1102	2	1.3700455601836943
1102	3	1.0732664753116339
1102	4	1.6784473821215187
1102	5	1.42959922793262
1102	6	1.1175286883214142
1102	7	0.9896740756793321
1102	8	0.7923221239569784
1102	9	1.0432417313513724
1102	10	1.3625696580833875
1102	11	1.0240995348134163
1102	12	1.0618103823211769
1102	13	1.061279010653557
1102	14	1.8785743105073323
1102	15	1.398988870163926
1102	16	1.637625120808373
1102	17	1.301077854469817
1102	18	1.55975616598775
1102	19	1.4417433815164529
1102	20	1.3939645793408104
1102	21	1.3858147444342634
1102	22	1.7098903834120378
1102	23	3.3430540435785616
1102	24	2.181737918148144
1102	25	2.5542031957386584
1103	1	0.8529354476666988
1103	2	1.187881653483874
1103	3	0.8862593815191069
1103	4	1.324475039246309
1103	5	1.1836404231638085
1103	6	0.793196856153557
1103	7	0.7923160824731426
1103	8	0.8135820744556597
1103	9	0.9420580041247888
1103	10	1.2114695917070435
1103	11	0.964045138595246
1103	12	1.0902081222878266
1103	13	0.8070965871458569
1103	14	1.6714262360401264
1103	15	1.142179581666845
1103	16	1.3211439809257186
1103	17	1.8302170666960151
1103	18	1.9766787989856454
1103	19	1.3264439689994205
1103	20	1.1168811765678655
1103	21	1.1815747535002998
1103	22	1.4623739726482157
1103	23	2.9479968427934793
1103	24	1.8773824114357538
1103	25	2.0283766103488787
1104	1	0.7666811460066612
1104	2	1.2034249694525911
1104	3	0.9390494661399273
1104	4	1.383825231677264
1104	5	1.290256181574513
1104	6	0.8449555746694415
1104	7	0.7900231463455825
1104	8	0.7739739560371888
1104	9	0.8685210377675929
1104	10	1.294663861102876
1104	11	0.9594743755250974
1104	12	1.1042650135307923
1104	13	0.8712711141861362
1104	14	1.8013393253804537
1104	15	1.184027800988396
1104	16	1.5083108398200054
1104	17	2.022339081341599
1104	18	2.524353157572591
1104	19	1.7445049819529643
1104	20	1.1571252884434031
1104	21	1.3012489769491893
1104	22	1.6535377271211473
1104	23	3.2165462765744266
1104	24	2.1265736946560168
1104	25	2.3274556069863834
1105	1	0.6690447519653411
1105	2	0.997878453317167
1105	3	0.7182346286017456
1105	4	0.9407307697773817
1105	5	0.8981405273552809
1105	6	0.6829844171799877
1105	7	0.5351598027041504
1105	8	0.5453904291234366
1105	9	0.716224286275299
1105	10	1.1502885441361954
1105	11	0.6696056143037481
1105	12	0.7380952680368154
1105	13	0.8088853314897477
1105	14	1.4568967349684385
1105	15	0.8309233673896372
1105	16	1.3038485683274814
1105	17	1.683057400704108
1105	18	1.533287701402891
1105	19	1.1708687948508114
1105	20	1.044282417934852
1105	21	0.9760237666581943
1105	22	1.4568112642652693
1105	23	2.926425672574375
1105	24	1.8699141154128895
1105	25	1.9564085822469508
1106	1	-0.4002524900623712
1106	2	0.10005489058286443
1106	3	-0.13763062254901826
1106	4	-2.53425165738059
1106	5	-0.2683095064557506
1106	6	-0.2678496254930991
1106	7	-0.20299354369883105
1106	8	-0.09963130336600301
1106	9	-0.07271271990559569
1106	10	-0.049308355628902234
1106	11	-0.008372797052011549
1106	12	-0.08786908801120319
1106	13	-0.12000360518277375
1106	14	0.6043491919038573
1106	15	-0.06516385767324095
1106	16	-0.867429177484695
1106	17	-5.378601401436072
1106	18	-6.474262503539453
1106	19	-2.5479125322620817
1106	20	-1.252106548396469
1106	21	-0.9918659426261982
1106	22	-1.3817829232021026
1106	23	-5.0147103115640945
1106	24	-2.0752993983250683
1106	25	-0.6913797606562895
1107	1	0.6809862521880916
1107	2	0.9615028778790915
1107	3	0.6307193145239296
1107	4	0.8953616540957654
1107	5	1.027755633532891
1107	6	0.5770539245307447
1107	7	0.4758931614062618
1107	8	0.481056220257976
1107	9	0.5313195584018189
1107	10	0.8678261796425843
1107	11	0.6120919708526102
1107	12	0.7501005769171272
1107	13	0.6910620652289765
1107	14	1.49034175278846
1107	15	0.9803783254383447
1107	16	-5.3531091276927185
1107	17	-0.7413490627648542
1107	18	0.8858119718959117
1107	19	0.7639517043399735
1107	20	0.7017645051044425
1107	21	0.7344314274096035
1107	22	0.8419487197152264
1107	23	2.713484580727034
1107	24	1.7760202558940321
1107	25	1.4444079139962795
1108	1	0.2671405541697318
1108	2	0.5589387397034429
1108	3	0.33995023688434856
1108	4	0.7708892391557285
1108	5	0.6708632145901134
1108	6	0.20904553943603332
1108	7	0.12394718625884593
1108	8	0.2751227490367327
1108	9	0.2746422153935413
1108	10	0.6515459516717073
1108	11	0.37323357731795426
1108	12	0.36667221417221896
1108	13	0.19553995010254965
1108	14	0.8668908550569867
1108	15	0.5292702187546965
1108	16	0.8207057556545436
1108	17	0.9869695594386627
1108	18	0.8329217922071663
1108	19	0.748347436937145
1108	20	0.5701842925889764
1108	21	0.5344114315263013
1108	22	0.9059140013064066
1108	23	2.630594789204153
1108	24	1.4153767837397666
1108	25	1.6260555569748618
1109	1	0.33503725963370456
1109	2	0.6946754512532678
1109	3	0.5503183491540966
1109	4	0.5274632565501705
1109	5	0.4386137636320555
1109	6	0.3137395192062655
1109	7	0.2960833877631046
1109	8	0.2544556479710707
1109	9	0.3169765661163453
1109	10	0.803148091246733
1109	11	0.48737280255205917
1109	12	0.4694535230711381
1109	13	0.4462849514924798
1109	14	-0.7814179215801715
1109	15	0.10115125528331248
1109	16	-0.12130704383015001
1109	17	0.8838909787218512
1109	18	1.7042434422692736
1109	19	0.8084352694283368
1109	20	0.549040401298214
1109	21	0.507196174381292
1109	22	0.4379594589219309
1109	23	-4.070873920204249
1109	24	-1.3149044522853472
1109	25	-0.05195178515025134
1110	1	0.4440522422106028
1110	2	0.7304526590027862
1110	3	0.5653099491145639
1110	4	1.1128269933002386
1110	5	0.6440083506551701
1110	6	0.3939506588187882
1110	7	0.41283207834517555
1110	8	0.31057942717249176
1110	9	0.37715290249603584
1110	10	0.6152071965888197
1110	11	0.32782851006740543
1110	12	0.3976528209911123
1110	13	0.18755694520211819
1110	14	1.043357855369777
1110	15	0.4728647344464889
1110	16	0.9429333648715144
1110	17	1.119644984316146
1110	18	1.731138349396197
1110	19	0.9980857332566089
1110	20	0.5784076269649532
1110	21	0.4744046543393523
1110	22	0.8851163785350593
1110	23	2.1886429593636763
1110	24	1.3017598511988453
1110	25	1.4544868988578727
1111	1	0.35061083020961803
1111	2	0.7307166432760646
1111	3	0.37873723429757433
1111	4	0.8894930642577137
1111	5	0.7829990490816527
1111	6	0.344071928189976
1111	7	0.20042651952740442
1111	8	-0.1029438522917161
1111	9	0.1747831713310859
1111	10	0.4617678270193437
1111	11	0.2194303720841404
1111	12	0.26241815412019776
1111	13	0.22244320729714673
1111	14	-0.4267736261589832
1111	15	0.021889218094273133
1111	16	0.3294054899364056
1111	17	0.912078796769876
1111	18	1.4660235902754692
1111	19	0.6338224978748883
1111	20	0.5056694922155387
1111	21	0.37535353398056515
1111	22	0.6049368147643861
1111	23	0.5623783113001579
1111	24	0.30729484060373125
1111	25	-0.0455915645528151
1112	1	0.1771501821041639
1112	2	0.5862314686271013
1112	3	0.4153194674850198
1112	4	0.9565469380109235
1112	5	0.6190036742383853
1112	6	0.05247125574476996
1112	7	0.060640949466439054
1112	8	0.09976440635622907
1112	9	0.15981026599018833
1112	10	0.4850215469807253
1112	11	0.257080697223401
1112	12	0.2021261240311958
1112	13	0.08819310342227027
1112	14	0.4883368735705602
1112	15	0.14321658307957108
1112	16	0.5790838047857676
1112	17	0.7993504942111187
1112	18	0.9155979128754552
1112	19	0.2923551776320821
1112	20	0.21800835201647573
1112	21	0.30883135874842793
1112	22	0.3174612911729682
1112	23	1.961078089599816
1112	24	0.9814733068639327
1112	25	1.0176579232828402
1113	1	0.332426703628542
1113	2	0.7551814610313592
1113	3	0.48114683863566654
1113	4	0.5580287167628342
1113	5	0.5886860260422324
1113	6	0.14919217035320287
1113	7	0.03360969807016545
1113	8	0.10856157407911837
1113	9	0.17433145404529427
1113	10	0.4542860924182861
1113	11	0.11098681711587233
1113	12	0.13103315784970704
1113	13	0.07448006969961796
1113	14	0.8754761063438679
1113	15	0.1627552657100182
1113	16	0.5738006623749712
1113	17	1.089676526155472
1113	18	1.4775741215527987
1113	19	0.5942207240826747
1113	20	0.22644522978516335
1113	21	0.21443392846473586
1113	22	0.5036341182427293
1113	23	0.41889467561081517
1113	24	0.30480271099227707
1113	25	0.6743392912960786
1114	1	-0.4144448358962869
1114	2	-3.0216153533318213
1114	3	-1.0913821484647883
1114	4	0.053482463813075753
1114	5	0.14896733767762527
1114	6	-0.11539366591062361
1114	7	-0.1346238885004496
1114	8	-0.025474890963721464
1114	9	0.0055414180432933335
1114	10	0.12433079246253698
1114	11	-0.23619727302860838
1114	12	-0.09067705193623254
1114	13	-0.052882137319350875
1114	14	0.6282806412218349
1114	15	-0.05253399032223882
1114	16	0.22235332711418465
1114	17	0.6451424842240101
1114	18	0.7765498510485926
1114	19	0.1727021605614638
1114	20	-0.0858311041922164
1114	21	-0.09903826534237226
1114	22	0.0327373801280757
1114	23	1.8423208590405693
1114	24	0.7081802352424802
1114	25	0.6405999824686575
1115	1	-0.038714512400453316
1115	2	0.24624674865787455
1115	3	0.03145015415751473
1115	4	0.19290698655382243
1115	5	0.02080645202011766
1115	6	-0.27815048927715935
1115	7	-0.5021526819762414
1115	8	-0.44543970374417796
1115	9	-0.40351451109558667
1115	10	-0.3324381827165439
1115	11	-0.31577431860969085
1115	12	-0.25861134950053355
1115	13	-0.5610500397509526
1115	14	0.3543769260505627
1115	15	-0.28927918112689355
1115	16	0.18148770366222067
1115	17	0.5328814583484629
1115	18	0.9667107709991996
1115	19	0.25201034202454053
1115	20	-0.09132191391714173
1115	21	-0.04013267383778896
1115	22	0.06515468855710083
1115	23	1.8322997593925905
1115	24	0.5018562286127946
1115	25	0.1616173818291884
1116	1	-0.27424524647996407
1116	2	-0.3644545818136393
1116	3	-0.2333654870780002
1116	4	0.4639281486693889
1116	5	-0.1364673802379457
1116	6	-0.3836191339773336
1116	7	-0.42638779924685366
1116	8	-0.36498799054370323
1116	9	-0.5648736205537546
1116	10	-0.058861140577214144
1116	11	-0.4985834114040273
1116	12	-0.3996001885687832
1116	13	-0.5355097675363325
1116	14	0.1465947159748282
1116	15	-0.577787379890971
1116	16	-0.1794132776471784
1116	17	0.197518850890674
1116	18	0.6492428926301983
1116	19	-0.2209690802519546
1116	20	-0.34030315740717754
1116	21	-0.5022997038131649
1116	22	-0.06427805551675903
1116	23	1.2579796448165084
1116	24	0.15492271892913578
1116	25	0.35934365929650625
1201	1	1.2734230204182637
1201	2	1.6258548396797146
1201	3	1.3973364996227602
1201	4	1.9272512062093412
1201	5	1.6556261820630525
1201	6	1.2331108547009322
1201	7	1.206356243456149
1201	8	1.1286153749733217
1201	9	1.4715691791119312
1201	10	1.7869485816523536
1201	11	1.287039931114819
1201	12	1.5095511282158398
1201	13	1.5361606085229198
1201	14	2.375207101872853
1201	15	1.8452318240815089
1201	16	2.0945744640481223
1201	17	2.6546267218232487
1201	18	3.215924475312704
1201	19	2.246341823008869
1201	20	1.9156815102473317
1201	21	1.9316451691161873
1201	22	2.128421123175805
1201	23	3.776910592815149
1201	24	2.521686260116354
1201	25	2.6687832292241183
1202	1	0.9341436748370384
1202	2	1.316263952979149
1202	3	1.0568981474824248
1202	4	1.7367905294229473
1202	5	1.4526756177749078
1202	6	1.0520417639253665
1202	7	0.9609425617098992
1202	8	0.9013635642941367
1202	9	1.118952078237733
1202	10	1.5095301857533698
1202	11	1.1325289658658733
1202	12	1.159845020559878
1202	13	1.1074990833609135
1202	14	2.0544667830151866
1202	15	1.6052766452519194
1202	16	1.8236440829588005
1202	17	2.1422995327481473
1202	18	2.7170500827950406
1202	19	1.968241578121372
1202	20	1.6426020645860717
1202	21	1.5666178697655369
1202	22	1.8591435080964516
1202	23	3.4656849091985507
1202	24	2.1982171780909567
1202	25	2.3262372195544003
1203	1	1.0619079089718184
1203	2	1.3570355837566268
1203	3	1.1186805611058688
1203	4	1.6936609006708778
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2211	15	-0.4527825202551412
2211	16	-0.5840599878863983
2211	17	-0.09021412442365317
2211	18	-5.094579287868719
2211	19	-1.806619555526325
2211	20	-1.0332259296248978
2211	21	-0.8590876073626958
2211	22	-0.5371472155164767
2211	23	-5.104584169456597
2211	24	-2.258460125844838
2211	25	-1.4707695938705214
2212	1	0.5588109085716226
2212	2	0.725395732281612
2212	3	0.4861652508134533
2212	4	1.0587427875999254
2212	5	0.9574934885783009
2212	6	0.4754870435262575
2212	7	0.3910746091008903
2212	8	0.395466719905734
2212	9	0.18480477076225554
2212	10	-3.394499637164781
2212	11	-0.7846984250590552
2212	12	-0.09032405173245905
2212	13	0.1297576023427922
2212	14	0.9038677761563108
2212	15	0.6115662448512182
2212	16	0.7465206397753619
2212	17	1.2984346228887773
2212	18	1.803784996195823
2212	19	0.9498052513534461
2212	20	0.5463014656343717
2212	21	0.40950309808760466
2212	22	0.7338242565819755
2212	23	1.9619156727433662
2212	24	1.0469446401751838
2212	25	0.9166106348808967
2213	1	-1.4443688327570356
2213	2	-3.818020190955835
2213	3	-1.6698779474203285
2213	4	-3.3229019943289337
2213	5	-1.3149688810803504
2213	6	-0.9682684512455069
2213	7	-0.8488467893951821
2213	8	-0.7522058127112317
2213	9	-0.8197291396454105
2213	10	-0.7135130699250709
2213	11	-0.8635806608961616
2213	12	-0.8173899358149654
2213	13	-1.096365646942509
2213	14	-6.117612289133373
2213	15	-2.711001838855438
2213	16	-1.9551743016466148
2213	17	-0.8726880731668203
2213	18	-0.054289112568277176
2213	19	-0.7346296952663209
2213	20	-0.782015060666339
2213	21	-1.1255147124779725
2213	22	-0.9525596882631646
2213	23	-5.476011697202626
2213	24	-3.0083171747098696
2213	25	-2.17602257554109
2214	1	-0.16298445220814273
2214	2	0.05306752178416119
2214	3	-0.2651865047861435
2214	4	0.21358555019200764
2214	5	-0.0927045694805031
2214	6	-0.28172618361106316
2214	7	-0.3178062195271636
2214	8	-0.4506293301509743
2214	9	-0.3699886331191351
2214	10	0.10370550062828698
2214	11	-0.4307962201206905
2214	12	-0.43336783889171215
2214	13	-0.4666706218654113
2214	14	0.09979942182059887
2214	15	-0.30709209564909656
2214	16	0.09999781137167219
2214	17	0.3620871674798849
2214	18	0.8835404462201311
2214	19	-3.60150184647523E-4
2214	20	-0.19335925555123623
2214	21	-0.2757526074552814
2214	22	0.3025816884332997
2214	23	1.6512948249202566
2214	24	0.6782014176496602
2214	25	0.5200126466222912
2215	1	-2.4853047785523508
2215	2	-4.4572543427227345
2215	3	-2.487717813136225
2215	4	-3.909668773908386
2215	5	-4.976345178561214
2215	6	-2.4551173169753184
2215	7	-1.7680585495661312
2215	8	-2.100474760658134
2215	9	-1.8370683043963254
2215	10	-3.4729409875639377
2215	11	-2.172844037951485
2215	12	-1.9697312734345687
2215	13	-1.8572706242440091
2215	14	-1.1017569768172564
2215	15	-1.7398817598301264
2215	16	-2.5953655795634774
2215	17	-6.896360556792558
2215	18	-3.2392517154931006
2215	19	-2.4206911723096134
2215	20	-2.188174140843145
2215	21	-2.219360788260758
2215	22	-1.959074611492568
2215	23	-6.580455152284806
2215	24	-3.9790285416365982
2215	25	-3.6653222407310793
2216	1	-1.2345461187760343
2216	2	-0.8185336556393352
2216	3	-1.1080419543209032
2216	4	-3.6267111763336075
2216	5	-1.7989919319333367
2216	6	-1.5459064321343448
2216	7	-1.5054037619519463
2216	8	-1.2750343954426668
2216	9	-1.4402820012220232
2216	10	-0.9977959118848005
2216	11	-1.4970602711687135
2216	12	-2.4055549623038743
2216	13	-1.6786317431834945
2216	14	-7.3842599234845245
2216	15	-3.906629028421378
2216	16	-2.1639884388505806
2216	17	-1.281394134007698
2216	18	-0.5109398872514959
2216	19	-0.9520834161519716
2216	20	-1.5326302802652734
2216	21	-1.7002754150966837
2216	22	-1.2684615957090202
2216	23	-0.1659022749829404
2216	24	-0.8963871755663249
2216	25	-0.9505940425655623
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102669.0
3	3.0
4	913.0
5	5160.0
6	6270.0
7	5629.0
8	5246.0
9	5096.0
10	4629.0
11	4723.0
12	4554.0
13	4523.0
14	4502.0
15	4771.0
16	4795.0
17	4992.0
18	5671.0
19	6818.0
20	8442.0
21	10478.0
22	12940.0
23	15594.0
24	18396.0
25	21692.0
26	24514.0
27	28581.0
28	31753.0
29	38524.0
30	45375.0
31	51491.0
32	66193.0
33	86947.0
34	113804.0
35	149593.0
36	211603.0
37	296288.0
38	747201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.60528035248072	17.847163203059182	12.221948301221245	25.325608143238853
2	14.387182495043527	19.792784410852235	40.91771534902332	24.902317745080925
3	19.168376487497817	24.391884914819205	28.96341562236784	27.476322975315142
4	11.012001365022728	16.220248361903273	39.034371438983065	33.733378834090935
5	12.709450254725219	37.59287676967117	36.08647414175521	13.6111988338484
6	32.62333364506837	36.12438042662019	17.995753613271393	13.256532315040042
7	28.470440409973975	31.133474880344007	21.854290531875392	18.541794177806626
8	25.994710802574595	35.33182954190846	20.46563827334372	18.20782138217322
9	26.780057173707505	15.079218088894281	19.423595683542512	38.71712905385571
10	14.316083680349918	28.672823056502615	34.749414127344934	22.261679135802524
11	34.80884548003452	22.616216836857525	23.323138549062747	19.251799134045207
12	23.186623011294717	24.731058340293377	31.14042560283859	20.94189304557332
13	28.916958366916862	21.22199758615246	25.276604058171227	24.584439988759453
14	21.328504104119492	21.00700818508979	28.1995071074951	29.464980603295608
15	24.65196321498069	28.31213097266958	23.21814749842701	23.81775831392272
16	22.925572426309735	27.608275440590585	26.744525750213555	22.721626382886125
17	22.466155290025746	27.35264662182591	27.52270937326806	22.65848871488028
18	22.163188207189023	26.82864497490694	28.861599288875723	22.146567529028317
19	23.60952584248956	27.00805365336183	27.412611633137175	21.96980887101143
20	23.914116417128195	26.57082376864362	27.286936987407483	22.2281228268207
21	23.66562641799476	26.375952660968448	27.115578727069	22.84284219396779
22	23.419190408407648	26.5968776944171	26.696851855796417	23.28708004137884
23	21.762573272708753	26.25978126996768	28.778278866900646	23.19936659042292
24	23.083662538081782	27.023885654853885	26.925205267708176	22.967246539356157
25	23.161685718730784	26.1755358161519	27.069365318081807	23.59341314703551
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	306.0
2	442.5
3	579.0
4	579.0
5	579.0
6	1166.5
7	1754.0
8	1754.0
9	1754.0
10	2742.0
11	3730.0
12	3730.0
13	3730.0
14	6436.5
15	9143.0
16	9143.0
17	9143.0
18	16256.0
19	23369.0
20	23369.0
21	23369.0
22	35753.5
23	48138.0
24	48138.0
25	48138.0
26	67039.0
27	85940.0
28	85940.0
29	85940.0
30	109106.5
31	132273.0
32	132273.0
33	132273.0
34	155183.0
35	178093.0
36	178093.0
37	178093.0
38	200843.5
39	223594.0
40	223594.0
41	223594.0
42	242643.5
43	261693.0
44	261693.0
45	261693.0
46	283518.5
47	305344.0
48	305344.0
49	305344.0
50	296187.5
51	287031.0
52	287031.0
53	287031.0
54	257602.5
55	228174.0
56	228174.0
57	228174.0
58	196477.5
59	164781.0
60	164781.0
61	164781.0
62	134570.0
63	104359.0
64	104359.0
65	104359.0
66	80886.5
67	57414.0
68	57414.0
69	57414.0
70	42337.5
71	27261.0
72	27261.0
73	27261.0
74	19204.5
75	11148.0
76	11148.0
77	11148.0
78	7639.0
79	4130.0
80	4130.0
81	4130.0
82	2808.5
83	1487.0
84	1487.0
85	1487.0
86	976.5
87	466.0
88	466.0
89	466.0
90	294.5
91	123.0
92	123.0
93	123.0
94	73.0
95	23.0
96	23.0
97	23.0
98	21.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11160109851400661
2	0.04980621401952348
3	0.036567759363776535
4	0.03249438870046978
5	0.03661404766676865
6	0.019394798953699197
7	0.019950258589604666
8	0.023097863193068974
9	0.02957822561196608
10	0.03582714651590258
11	0.020598294831494376
12	0.019672528771651935
13	0.015552869805353057
14	0.021755502406297433
15	0.021848079012281675
16	0.02258869186015563
17	0.02693979234141512
18	0.019117069135746467
19	0.033883037790233446
20	0.02402362925291142
21	0.02930049579401335
22	0.03772496693857959
23	0.02930049579401335
24	0.04054855342109904
25	0.031337181125666726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2160373.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.33025026741528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29951803711579	61.083745048167735
2	11.176534889193604	16.391562010941307
3	2.9853788280991473	6.567557298226601
4	1.1080025044892492	3.250004038044782
5	0.4947151158935459	1.8138791629773536
6	0.26235111281391205	1.154296365634744
7	0.16409405519420728	0.8423140694350393
8	0.1111176573063002	0.6518628495519904
9	0.07582545872254152	0.5004269878288983
>10	0.2981371953755976	3.6266663495191613
>50	0.014919624850622846	0.746429452260302
>100	0.007040394858753218	1.040332973043264
>500	0.0014702135133824595	0.6746163953539759
>1k	7.670679200251931E-4	1.0364867797429118
>5k	1.278446533375322E-4	0.6198202192719122
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7068	0.32716572554831963	No Hit
CCCATGTACTCTGCGTTGATACCAC	6155	0.2849045049165121	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3052	0.14127190073195695	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2997	0.1387260440673902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	4.628830299212219E-5
13	0.0	0.0	0.0	4.628830299212219E-5	4.628830299212219E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTGC	155	1.8189894E-12	12.255944	8
TAGGACG	325	0.0	11.109371	4
CACCGTA	60	0.0058844895	11.081416	7
TCGAACT	215	0.0	11.045623	19
TACACCG	70	0.0014907944	10.858029	5
TGCGCCA	195	1.8189894E-12	10.716591	12
GTATAAG	260	0.0	10.607828	1
ACACCGT	135	5.696893E-8	10.554706	6
GCGTGCG	145	1.4479156E-8	10.480945	9
ATTACAC	215	1.8189894E-12	10.164327	3
GTATAAT	385	0.0	10.128028	1
TTAGACA	255	0.0	10.060344	4
ACCGTGC	85	6.60566E-4	10.057083	8
AGGCGTG	200	2.5465852E-11	9.973274	7
GCGTTTA	105	4.1108986E-5	9.95135	13
TACTCCG	115	1.0257363E-5	9.913854	5
TGTACCG	125	2.5680492E-6	9.880807	5
CGTGCGC	165	1.0253643E-8	9.786185	10
GTAGACC	515	0.0	9.778176	3
GTCTTAC	215	1.0913936E-11	9.731639	1
>>END_MODULE