FastQCFastQC Report
Thu 26 May 2016
SRR1513027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160373
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC84790.3924785210702041No Hit
CCCATGTACTCTGCGTTGATACCAC79570.3683160269083163No Hit
GTATCAACGCAGAGTACTTTTTTTT45720.21163012127998265No Hit
GAGTACATGGGAAGCAGTGGTATCA33810.15650075241636513No Hit
TATCAACGCAGAGTACTTTTTTTTT31780.14710422690896433No Hit
GGTATCAACGCAGAGTACTTTTTTT27810.1287277706210918No Hit
CATGTACTCTGCGTTGATACCACTG23250.10762030445668411No Hit
GTATCAACGCAGAGTACATGGGAAG22700.10507444779211737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC707.1237246E-613.5973753
CGGTACT657.898055E-411.7146624
GCACCGT907.307164E-611.633316
AGGACCG1104.849753E-711.2487385
TAGGACC3400.010.9178924
TTGGACA2600.010.2503294
ATCGTGC750.002676260410.1205038
CTGGTCG856.6738325E-410.04585559
ATAATAC2600.09.8842463
TTAGAAC1856.2573235E-109.7754113
GGCGTGC1752.6302587E-99.7590578
TAGGACG2750.09.691224
ATAGCCC2950.09.6794883
GTCCTAT2303.6379788E-129.5274571
GTCCTAA2303.6379788E-129.5274571
GCCTAAG1701.5885234E-89.5274571
CTTAGAC2900.09.5181643
CTTGGAC3100.09.5181633
ATTGGAC1106.6965746E-59.5181633
ACTCCGC800.0045640949.4879728
Produced by FastQC (version 0.11.5)