FastQCFastQC Report
Thu 26 May 2016
SRR1513026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066061
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC34160.16533877750947334No Hit
GTATCAACGCAGAGTACTTTTTTTT28530.13808885604055252No Hit
CCCATGTACTCTGCGTTGATACCAC27310.1321838997009285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC8000.011.5323011
TACACCG1001.9272939E-611.40152555
ACCGTCT1104.9775736E-711.2260578
TAATACT2900.010.8125774
TCTAGAC2200.010.7968993
CTAGACA2050.010.6607374
GCGTTTA1002.4042165E-510.4481113
AGGCGTG1752.3646862E-1010.3131697
GTATAAT2600.010.2428051
ATAACAC2250.010.1346893
CCTACAC2650.010.039083
GTCCTAA3900.09.9989281
CGTTTAT1054.1141568E-59.95058114
GTATTAG2201.8189894E-129.9435011
GTGTCGG1151.012416E-59.92470551
TAGGACC3750.09.882044
TCCTACA9850.09.6459612
GTACTAG2002.582965E-109.5111761
TAATACC1804.1673047E-99.5019624
TAAACAG3900.09.5012715
Produced by FastQC (version 0.11.5)