##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513026_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2066061
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.247812141074245	31.0	30.0	34.0	25.0	34.0
2	29.020921453916415	31.0	30.0	34.0	16.0	34.0
3	29.37551021000832	31.0	30.0	34.0	23.0	34.0
4	32.224598886480116	35.0	33.0	37.0	19.0	37.0
5	32.46554288571344	35.0	33.0	37.0	22.0	37.0
6	32.91949221247582	37.0	35.0	37.0	26.0	37.0
7	32.91930247945245	37.0	35.0	37.0	26.0	37.0
8	32.896391248854705	37.0	35.0	37.0	26.0	37.0
9	34.28145441978722	39.0	35.0	39.0	26.0	39.0
10	33.7857570517037	39.0	34.0	39.0	19.0	39.0
11	34.147833486039374	39.0	35.0	39.0	25.0	39.0
12	34.03346416199715	39.0	35.0	39.0	25.0	39.0
13	34.083956378829086	39.0	35.0	39.0	25.0	39.0
14	34.48615699149251	40.0	34.0	41.0	17.0	41.0
15	34.9635131779749	40.0	34.0	41.0	23.0	41.0
16	34.524424496662974	40.0	34.0	41.0	17.0	41.0
17	34.039807633946914	39.0	33.0	41.0	11.0	41.0
18	33.42566845799809	39.0	32.0	41.0	10.0	41.0
19	34.3512355153115	39.0	34.0	41.0	18.0	41.0
20	34.621907097612315	39.0	34.0	41.0	18.0	41.0
21	34.65796992441172	40.0	34.0	41.0	18.0	41.0
22	34.23984577415672	40.0	34.0	41.0	10.0	41.0
23	32.48068135451954	39.0	31.0	41.0	2.0	41.0
24	33.614221458127325	39.0	33.0	41.0	2.0	41.0
25	33.3590329617567	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7057230325456842
1101	2	1.0860806296308283
1101	3	0.8107918555750793
1101	4	0.8646121808010463
1101	5	1.0779155557661326
1101	6	0.7382955678828935
1101	7	0.7613026865111223
1101	8	0.8359689562120352
1101	9	0.9701857533881864
1101	10	1.3211631653499083
1101	11	0.9364533872766785
1101	12	0.9708036355180525
1101	13	0.8742592955913153
1101	14	0.057249781199217864
1101	15	0.6022874274097418
1101	16	1.223520182784327
1101	17	1.8797140073247007
1101	18	-4.418857884113699
1101	19	-0.7991755739580881
1101	20	0.21431280012998144
1101	21	0.6272917406403948
1101	22	1.2016423329758652
1101	23	3.0947563104622873
1101	24	1.8972630557830712
1101	25	2.11733544839538
1102	1	0.7772465595834959
1102	2	1.1043538222206983
1102	3	0.8550289001814306
1102	4	1.458009457630169
1102	5	1.1488948517625275
1102	6	0.7342550248612554
1102	7	0.6420565823421427
1102	8	0.5960157372957511
1102	9	0.7179160864689749
1102	10	1.1630953345312633
1102	11	0.7391600822386835
1102	12	0.7615457484402981
1102	13	0.6952124535992894
1102	14	1.6047510289430846
1102	15	1.0725082178242644
1102	16	1.3891842870663496
1102	17	1.0832324916806897
1102	18	1.4398620161193705
1102	19	1.1804274178227985
1102	20	1.0800513527646132
1102	21	1.093806063513128
1102	22	1.536460455957183
1102	23	2.9840892505938044
1102	24	1.8903741073978253
1102	25	2.1112829156396344
1103	1	0.6999517976227807
1103	2	0.9173819087977861
1103	3	0.7840443636572196
1103	4	1.2173790270152622
1103	5	1.067273709128017
1103	6	0.7630262956439182
1103	7	0.6918197666714079
1103	8	0.5965490032493364
1103	9	0.7497060171426355
1103	10	1.062943818442733
1103	11	0.791113844652628
1103	12	0.9270002569494977
1103	13	0.705245999172945
1103	14	1.4250670835524915
1103	15	0.9565713757376031
1103	16	1.1529495899704258
1103	17	1.6382224068051698
1103	18	1.704633067800053
1103	19	1.1155505257490788
1103	20	1.0356374373150103
1103	21	0.9402788308153376
1103	22	1.2509991976933748
1103	23	2.807672221775711
1103	24	1.690308641845867
1103	25	1.9077815246070884
1104	1	0.6121761776725876
1104	2	0.9980926509223593
1104	3	0.8335590304567972
1104	4	1.253818445363045
1104	5	1.1816227680996079
1104	6	0.6802884218536889
1104	7	0.570269655328687
1104	8	0.5934591761109047
1104	9	0.6853271421757867
1104	10	1.0304513258433303
1104	11	0.6113853393151203
1104	12	0.835693889142938
1104	13	0.7026955300382838
1104	14	1.548713392866297
1104	15	0.9297668850037013
1104	16	1.1973364396034682
1104	17	1.7571752334505248
1104	18	2.3945230423273998
1104	19	1.606294572785366
1104	20	1.0000401650780617
1104	21	1.1364412007152467
1104	22	1.430271829813833
1104	23	3.0514465529628794
1104	24	1.9591805647906568
1104	25	2.073384190424079
1105	1	0.7447328678094465
1105	2	1.1720968795613338
1105	3	0.797357424131075
1105	4	1.0834448575569837
1105	5	1.1351066077873568
1105	6	0.7892640519061018
1105	7	0.5296119618258004
1105	8	0.54574432112328
1105	9	0.7230383673930305
1105	10	1.2273739420982608
1105	11	0.8081000924602648
1105	12	0.7357280977703056
1105	13	0.6763793465721051
1105	14	1.4241919872311826
1105	15	0.9377418474220889
1105	16	1.290027718492567
1105	17	1.7637180468968694
1105	18	1.6144362934265644
1105	19	1.3019244859233083
1105	20	1.1266448151882216
1105	21	0.8538820298000616
1105	22	1.3477949162850607
1105	23	2.870520256451556
1105	24	1.7690330846956357
1105	25	1.8495294613207136
1106	1	-0.6089215043624492
1106	2	-0.2015771668352393
1106	3	-0.4035708635227735
1106	4	-2.758346567270749
1106	5	-0.705665661519042
1106	6	-0.5984289155681211
1106	7	-0.5321026264874718
1106	8	-0.4130117536705171
1106	9	-0.42492830757041844
1106	10	-0.40091670070682284
1106	11	-0.411965377194619
1106	12	-0.3678105734903667
1106	13	-0.39479269462120925
1106	14	0.16612662637791686
1106	15	-0.41286123007667186
1106	16	-1.295695216685914
1106	17	-6.033103923059603
1106	18	-7.41205116974378
1106	19	-3.283321097581805
1106	20	-1.8503648031746707
1106	21	-1.4216460790629242
1106	22	-1.8968570741541484
1106	23	-5.3432615229451415
1106	24	-2.4609010621105476
1106	25	-1.0424035510919225
1107	1	0.3602045430819061
1107	2	0.7595014801872964
1107	3	0.4416944890474639
1107	4	0.8417427659493057
1107	5	0.7705970303751286
1107	6	0.26426276687036676
1107	7	0.20669483685842494
1107	8	0.2903319959903854
1107	9	0.2181790381239992
1107	10	0.574590769944713
1107	11	0.18991688416213037
1107	12	0.3258010955490178
1107	13	0.24180927647933004
1107	14	0.9447588012113073
1107	15	0.40109594308712104
1107	16	-6.193823321370406
1107	17	-1.3828813266600477
1107	18	0.3840417413926005
1107	19	0.41909994207655643
1107	20	0.2500384667070179
1107	21	0.2548020723366804
1107	22	0.3900826225698921
1107	23	2.2481699161904487
1107	24	1.2326983441226105
1107	25	0.9732098243301834
1108	1	0.38602727425677585
1108	2	0.5164800139887795
1108	3	0.36991921876138534
1108	4	0.8572436255241342
1108	5	0.6616830077194251
1108	6	0.17526889975466986
1108	7	0.16134035879358066
1108	8	0.2317680045618573
1108	9	0.27421404786731784
1108	10	0.7833573297254404
1108	11	0.26274430051633857
1108	12	0.40517337420574506
1108	13	0.24168171954767104
1108	14	0.8464439209669621
1108	15	0.4581000324122826
1108	16	0.712830516040853
1108	17	0.7933448860687164
1108	18	0.3999767359085382
1108	19	0.6189484627030382
1108	20	0.436687272460766
1108	21	0.5697474634046102
1108	22	0.8979053051936816
1108	23	2.696204590489934
1108	24	1.3268425982124867
1108	25	1.5361213002609801
1109	1	0.09764080316394086
1109	2	0.3804598026024024
1109	3	0.35549441681012794
1109	4	0.3263835889268307
1109	5	0.11664149419477354
1109	6	0.08367458779066794
1109	7	0.12906243431303466
1109	8	0.050678910065116156
1109	9	0.08602642062226096
1109	10	0.5294773213936139
1109	11	0.15188771311396465
1109	12	0.25549166322588235
1109	13	0.08088159591418531
1109	14	-1.1093649040446252
1109	15	-0.3140208865069951
1109	16	-0.5629638850646828
1109	17	0.5063258191879356
1109	18	1.4015050428241764
1109	19	0.441226319991479
1109	20	0.2870429202934801
1109	21	0.1792615972647269
1109	22	0.04869738817145475
1109	23	-4.382097509711418
1109	24	-1.7902770104606702
1109	25	-0.47908122795410435
1110	1	0.4317415599710621
1110	2	0.811517607973741
1110	3	0.4399944067313122
1110	4	1.0623564074790224
1110	5	0.7061942859149184
1110	6	0.31962980201031144
1110	7	0.3279569001469653
1110	8	0.3262040947874212
1110	9	0.3635373561379893
1110	10	0.7208009387479919
1110	11	0.2825939245764886
1110	12	0.2960004418749165
1110	13	0.21702006541232777
1110	14	1.0891506878842563
1110	15	0.5390022880513854
1110	16	0.8959884107776901
1110	17	1.219510299602554
1110	18	1.8147135780138015
1110	19	1.0409033499210807
1110	20	0.773830129603617
1110	21	0.6304993431038568
1110	22	1.0293338766222533
1110	23	2.38703165815911
1110	24	1.433008011746324
1110	25	1.7132641574038416
1111	1	0.3687461184687564
1111	2	0.7249176842513911
1111	3	0.49797168688822424
1111	4	1.0264603266973538
1111	5	0.7239108599587212
1111	6	0.23644103298630625
1111	7	0.15365656597820987
1111	8	-0.025422616919385632
1111	9	0.0935990139056031
1111	10	0.4234593964865425
1111	11	0.18724132205569788
1111	12	0.3446855604685055
1111	13	0.16365558537165015
1111	14	-0.434443260755458
1111	15	-0.07768717110375434
1111	16	0.3400567337590559
1111	17	0.8408595967584844
1111	18	1.4888986784460414
1111	19	0.5631001462597567
1111	20	0.33679133696985986
1111	21	0.2963117908899875
1111	22	0.6168466803707489
1111	23	0.38641426322249117
1111	24	0.30898345760735424
1111	25	-0.16001723448167127
1112	1	0.5165609232222259
1112	2	0.760758372182341
1112	3	0.5417671561910637
1112	4	1.1091511450086102
1112	5	0.9231028480853567
1112	6	0.31426246718437056
1112	7	0.1768435177882992
1112	8	0.261944665354342
1112	9	0.1809575906621248
1112	10	0.5202526005144179
1112	11	0.26326248106371253
1112	12	0.32921697910339276
1112	13	0.28529899192702857
1112	14	0.56425680123224
1112	15	0.2920343959040679
1112	16	0.7369184408208014
1112	17	1.0115997276835742
1112	18	0.9524212730361796
1112	19	0.4871953982375459
1112	20	0.3743555516622692
1112	21	0.3689120313315897
1112	22	0.5488768364295709
1112	23	2.0865376312601
1112	24	0.9397988535261774
1112	25	1.0135818214206367
1113	1	0.5768644224778825
1113	2	1.0935503651434182
1113	3	0.7663383107718538
1113	4	0.9828595102544071
1113	5	1.0716366741733836
1113	6	0.564377464524533
1113	7	0.49654811989375247
1113	8	0.46075088611999604
1113	9	0.5965392335251849
1113	10	0.8978742493307266
1113	11	0.6021376177577835
1113	12	0.514703564996374
1113	13	0.38162290091436546
1113	14	1.1702971888229499
1113	15	0.6952206938444618
1113	16	1.1917132394545504
1113	17	1.5197361409587131
1113	18	1.9780870485906092
1113	19	1.0111856053752177
1113	20	0.8419186165035271
1113	21	0.92502745304661
1113	22	1.1443280964892608
1113	23	1.0197246452393571
1113	24	1.081315311360676
1113	25	1.480756781954149
1114	1	-0.297397799621951
1114	2	-2.976926356414843
1114	3	-0.977287884818459
1114	4	0.1547365182035918
1114	5	0.3511145155935935
1114	6	0.09008495107701009
1114	7	0.09530250295996723
1114	8	0.1477919263065175
1114	9	0.2630326428622638
1114	10	0.3775251186006088
1114	11	0.1782824930125031
1114	12	0.12317986431160932
1114	13	0.1654987247472377
1114	14	0.8911119342838347
1114	15	0.36714933477266243
1114	16	0.6583835777997251
1114	17	0.9618578662804396
1114	18	0.9845905091476084
1114	19	0.43855980449034604
1114	20	0.38295995042432907
1114	21	0.13161444428966007
1114	22	0.436309150628162
1114	23	2.085058722087588
1114	24	0.9043805662669513
1114	25	0.7924937612642395
1115	1	0.3419865921944769
1115	2	0.6533597132368989
1115	3	0.44722856355193485
1115	4	0.4811211765445407
1115	5	0.5041487703402652
1115	6	0.1174735184442568
1115	7	0.0774802621233377
1115	8	0.013089164604821235
1115	9	0.037576695557973494
1115	10	0.2508418893387585
1115	11	0.17815716302976625
1115	12	-0.04067718970402723
1115	13	-0.10461594425469656
1115	14	0.9010605965845784
1115	15	0.2811317187710358
1115	16	0.7276593291450411
1115	17	1.330253452126719
1115	18	1.7124102751136903
1115	19	0.9432160513174281
1115	20	0.532702310054745
1115	21	0.44717630336164405
1115	22	0.744360729468653
1115	23	2.4438784626703836
1115	24	1.0893707937012138
1115	25	0.8567785236766667
1116	1	0.6273813841692402
1116	2	0.50372203207748
1116	3	0.5653026047626213
1116	4	1.3930062204171634
1116	5	0.7636451594756295
1116	6	0.6076970716336447
1116	7	0.48753337757241155
1116	8	0.5855677212448711
1116	9	0.5924516709104282
1116	10	1.1396429713657739
1116	11	0.6435819017658417
1116	12	0.8642507778598087
1116	13	0.6814695950334837
1116	14	1.5758224397276308
1116	15	0.7947162105387591
1116	16	1.3539456226479487
1116	17	1.558069741686019
1116	18	2.091072591489521
1116	19	1.296433479593091
1116	20	1.0226609021872548
1116	21	0.957120969633948
1116	22	1.4609288059746177
1116	23	3.114373200420559
1116	24	1.8819303175107365
1116	25	2.069692910445866
1201	1	0.8202750444783646
1201	2	1.1313936573642636
1201	3	0.8827449900083693
1201	4	1.5054451446418398
1201	5	1.3035159770457199
1201	6	0.7765950447958616
1201	7	0.7037946621699263
1201	8	0.7285044085035182
1201	9	0.915219384880622
1201	10	1.1245530646319466
1201	11	0.919843070270403
1201	12	1.0121318638513088
1201	13	0.9118226772287414
1201	14	1.7653891663272816
1201	15	1.2492156104833256
1201	16	1.5654198144596236
1201	17	1.9693165364322383
1201	18	2.4843162720423564
1201	19	1.7049497681595298
1201	20	1.3108102789857838
1201	21	1.2027505779798702
1201	22	1.4976803510992198
1201	23	3.15471840280685
1201	24	2.0109380275337614
1201	25	2.127138314108308
1202	1	0.883449005544616
1202	2	1.2168989056359472
1202	3	1.0242742274231844
1202	4	1.604430595323926
1202	5	1.4233712544645556
1202	6	1.0345350580244599
1202	7	0.8392431323545608
1202	8	0.8567922040153562
1202	9	1.0062501952428775
1202	10	1.5041965417698009
1202	11	1.1121146682408778
1202	12	1.1915664054775092
1202	13	1.1152467187439967
1202	14	2.011842899410695
1202	15	1.4771633974757776
1202	16	1.767506904160797
1202	17	2.127282967614626
1202	18	2.7046791987163914
1202	19	1.9557083255430356
1202	20	1.5731247735769713
1202	21	1.4747724665299984
1202	22	2.000711013050015
1202	23	3.5508569577501277
1202	24	2.2181140071386025
1202	25	2.3533208609203484
1203	1	0.7719049889154341
1203	2	1.0443030259029733
1203	3	0.8315529310314318
1203	4	1.4840148938188733
1203	5	1.215199482697841
1203	6	0.818058513292506
1203	7	0.637092005403467
1203	8	0.6106726621313783
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2211	18	-5.674248049414192
2211	19	-2.074978015141184
2211	20	-1.3932244122295927
2211	21	-1.1158186191708879
2211	22	-0.6869045520804491
2211	23	-5.011063429047169
2211	24	-2.2457839108657467
2211	25	-1.470914139029599
2212	1	0.34280072861563937
2212	2	0.6338442878707404
2212	3	0.32233031818336144
2212	4	0.7902374375706103
2212	5	0.766327229679753
2212	6	0.2420724621039838
2212	7	0.22083524746430783
2212	8	0.10542672287524368
2212	9	-0.13628649454511788
2212	10	-3.7172277259333413
2212	11	-1.0211361391306113
2212	12	-0.20292246526828706
2212	13	7.723410843709644E-4
2212	14	0.7137256497513746
2212	15	0.3624038583529554
2212	16	0.5000466398655234
2212	17	1.0128901968370698
2212	18	1.6139925795063164
2212	19	0.9054389488123604
2212	20	0.5103210608894528
2212	21	0.40996133557334247
2212	22	0.6452151503894044
2212	23	2.0556991511358618
2212	24	1.1329327066246861
2212	25	1.0582859827681261
2213	1	-1.1594280700417627
2213	2	-3.6898306731910004
2213	3	-1.4122024378778555
2213	4	-3.150966059858032
2213	5	-1.0172360618678304
2213	6	-0.6718637291786393
2213	7	-0.5725516757224938
2213	8	-0.517858087057391
2213	9	-0.4919468775376217
2213	10	-0.5133254705835313
2213	11	-0.5704646407812959
2213	12	-0.3551316295855429
2213	13	-0.7293906361767029
2213	14	-5.841740063971823
2213	15	-2.341505201218446
2213	16	-1.7691289537930146
2213	17	-0.5018928312216815
2213	18	0.29906432292509066
2213	19	-0.38249397468165824
2213	20	-0.4318078106759202
2213	21	-0.7217987442445803
2213	22	-0.5377261863532823
2213	23	-5.690673537913071
2213	24	-2.9865585294889527
2213	25	-2.233151457505251
2214	1	0.008473333539061656
2214	2	0.20806176165402945
2214	3	0.030433633289327133
2214	4	0.41296411856306037
2214	5	0.14048644088334328
2214	6	-0.09326911194123255
2214	7	-0.27833443340105646
2214	8	-0.3047353581279211
2214	9	-0.18888317377150798
2214	10	0.2875797106311495
2214	11	-0.25026205149031
2214	12	-0.097012817530576
2214	13	-0.23245100518781925
2214	14	0.38940028767516566
2214	15	0.05507029792709517
2214	16	0.5305844912955635
2214	17	0.6844535702559114
2214	18	1.130351462296204
2214	19	0.11718483575858585
2214	20	-0.0039874123891650015
2214	21	-0.13575680004731794
2214	22	0.3563110962075271
2214	23	1.7960319055186602
2214	24	0.8271196409123505
2214	25	0.6802048797870768
2215	1	-2.382967071703515
2215	2	-4.539525819354516
2215	3	-2.414139497185875
2215	4	-4.091191532322789
2215	5	-4.995483278425187
2215	6	-2.419854520879582
2215	7	-1.8305092174522777
2215	8	-2.0088033761952815
2215	9	-1.760381981155959
2215	10	-3.0296242498196087
2215	11	-2.0560732521092646
2215	12	-1.7889177359225172
2215	13	-1.758784469773012
2215	14	-1.0395950237716747
2215	15	-1.6519378604119836
2215	16	-2.351442886065371
2215	17	-6.957826205411969
2215	18	-3.0488258747516603
2215	19	-2.2110755639773316
2215	20	-1.9292895137017823
2215	21	-1.7660336427789716
2215	22	-1.510139448981107
2215	23	-6.761875426723211
2215	24	-3.729410435006489
2215	25	-3.389253503999271
2216	1	-0.44451779256676005
2216	2	0.016623771916606245
2216	3	-0.2555270374659777
2216	4	-2.906137443284809
2216	5	-0.6008233805005112
2216	6	-0.35808845451119
2216	7	-0.2893786146792223
2216	8	-0.1737852428598785
2216	9	-0.1718326091174447
2216	10	0.1931409574990255
2216	11	-0.2903987036934481
2216	12	-1.2690270412583402
2216	13	-0.5454866904749451
2216	14	-6.002940420081256
2216	15	-2.3627327341005753
2216	16	-0.7694813761590851
2216	17	0.20846483753663136
2216	18	0.8394774436681587
2216	19	0.47495302177798493
2216	20	-0.027741280584443473
2216	21	-0.42661336531756433
2216	22	0.14711757792930769
2216	23	1.311073057500984
2216	24	0.6441145065897871
2216	25	0.7123538130833822
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	98902.0
3	2.0
4	867.0
5	5014.0
6	6076.0
7	5403.0
8	4831.0
9	4707.0
10	4448.0
11	4297.0
12	4355.0
13	4314.0
14	4323.0
15	4363.0
16	4425.0
17	4772.0
18	5308.0
19	6349.0
20	7803.0
21	9854.0
22	12131.0
23	14861.0
24	17016.0
25	20540.0
26	23308.0
27	26839.0
28	29840.0
29	36422.0
30	42996.0
31	48723.0
32	62962.0
33	83335.0
34	108277.0
35	143525.0
36	204149.0
37	286374.0
38	714350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21274841215354	18.046385611342085	12.469579095321176	25.2712868811832
2	14.498489604469105	19.7216300843284	41.38425643891445	24.395623872288045
3	19.02892444935958	24.344579443822788	29.153522747796057	27.472973359021573
4	11.012818495430613	16.278835562549173	39.109169037099804	33.59917690492041
5	12.761223659745893	37.555876200805706	36.10677486829873	13.576125271149674
6	32.41239784529271	36.4189489145374	18.029770281712437	13.13888295845745
7	28.53664830092258	31.177742673994892	21.863836589577662	18.42177243550487
8	25.71707571184706	35.89237110646988	20.294915329729438	18.095637851953626
9	26.52976742654734	15.232166754139126	19.593621030574045	38.644444788739484
10	14.26378028816334	28.72961481866685	34.843099288379776	22.16350560479004
11	34.863961139084914	22.540850871625658	23.652573581916933	18.942614407372496
12	23.07131476938852	24.681826301778113	31.35382793615661	20.893030992676763
13	28.75082780495173	21.408875618191185	25.631891113150772	24.20840546370632
14	21.225468315651575	21.145394496691992	28.06601878200653	29.563118405649902
15	23.94470864416405	29.14066694520197	23.195519680552604	23.719104730081376
16	22.864545914029925	27.818417898134935	26.781215324106437	22.535820863728702
17	22.207197393329363	27.610083903421494	27.691518153253252	22.491200549995884
18	21.858743042155172	27.12734078408097	29.161853824503037	21.852062349260816
19	23.47093072518853	26.98287194509339	27.41156960768886	22.134627722029222
20	23.658574607225923	26.81340302212858	27.49747768648175	22.030544684163743
21	23.588316689599612	26.403936784475185	27.185609666115052	22.82213685981015
22	23.257164078868175	26.848318548334383	27.223378649464063	22.671138723333375
23	22.040377817618612	26.343768715736736	28.889278367186478	22.72657509945818
24	23.048790290169602	26.93973385705833	27.242173419988124	22.769302432783945
25	23.16300859693191	26.445345862375497	27.358878815990025	23.032766724702576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	212.0
1	212.0
2	367.5
3	523.0
4	523.0
5	523.0
6	1078.5
7	1634.0
8	1634.0
9	1634.0
10	2604.0
11	3574.0
12	3574.0
13	3574.0
14	6368.0
15	9162.0
16	9162.0
17	9162.0
18	15932.0
19	22702.0
20	22702.0
21	22702.0
22	35675.0
23	48648.0
24	48648.0
25	48648.0
26	67401.0
27	86154.0
28	86154.0
29	86154.0
30	110257.0
31	134360.0
32	134360.0
33	134360.0
34	156642.5
35	178925.0
36	178925.0
37	178925.0
38	199287.0
39	219649.0
40	219649.0
41	219649.0
42	237037.5
43	254426.0
44	254426.0
45	254426.0
46	267440.0
47	280454.0
48	280454.0
49	280454.0
50	272352.5
51	264251.0
52	264251.0
53	264251.0
54	238379.0
55	212507.0
56	212507.0
57	212507.0
58	183340.0
59	154173.0
60	154173.0
61	154173.0
62	126284.0
63	98395.0
64	98395.0
65	98395.0
66	76412.0
67	54429.0
68	54429.0
69	54429.0
70	40080.5
71	25732.0
72	25732.0
73	25732.0
74	18145.5
75	10559.0
76	10559.0
77	10559.0
78	7163.5
79	3768.0
80	3768.0
81	3768.0
82	2532.5
83	1297.0
84	1297.0
85	1297.0
86	854.5
87	412.0
88	412.0
89	412.0
90	243.5
91	75.0
92	75.0
93	75.0
94	51.0
95	27.0
96	27.0
97	27.0
98	20.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10173949365483399
2	0.05028893144974906
3	0.037946604674305356
4	0.03320327909001718
5	0.03780140082988837
6	0.02149016897371375
7	0.021296563847824437
8	0.025120265084138366
9	0.031412431675541044
10	0.037462591859582076
11	0.02119976128487978
12	0.02149016897371375
13	0.017279257485621192
14	0.0226033984475773
15	0.02391023304733016
16	0.02444264714352577
17	0.028605157350146
18	0.019699321559237603
19	0.0356233431636336
20	0.024055436891747148
21	0.02996039323137119
22	0.03833381492608399
23	0.030008794512843523
24	0.041383095658840664
25	0.03257406243087692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2066061.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.38645227866746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.09179938978843	66.62338925062475
2	9.549122423930543	14.779454135253085
3	2.3583060645585845	5.475028191703649
4	0.8470162289899721	2.6219032393595736
5	0.38074333219914075	1.4732187853824819
6	0.2215506386950164	1.028701075720811
7	0.13420976948412766	0.7270212545060085
8	0.09370309538133534	0.5801080095272906
9	0.05457419261459166	0.38009728371743134
>10	0.24982528763619777	3.2138409083355044
>50	0.010383615291293509	0.5363645741381822
>100	0.0069381417791570385	1.0893701399505544
>500	0.001323593540833903	0.7136319604131746
>1k	5.042261107938229E-4	0.7578711913675864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3416	0.16533877750947334	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2853	0.13808885604055252	No Hit
CCCATGTACTCTGCGTTGATACCAC	2731	0.1321838997009285	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.840128147232826E-5	0.0	0.0	0.0	0.0
7	4.840128147232826E-5	0.0	0.0	0.0	0.0
8	4.840128147232826E-5	0.0	0.0	0.0	0.0
9	4.840128147232826E-5	0.0	0.0	0.0	0.0
10	4.840128147232826E-5	0.0	0.0	0.0	0.0
11	4.840128147232826E-5	0.0	0.0	4.840128147232826E-5	0.0
12	4.840128147232826E-5	0.0	0.0	4.840128147232826E-5	0.0
13	4.840128147232826E-5	0.0	0.0	4.840128147232826E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	800	0.0	11.532301	1
TACACCG	100	1.9272939E-6	11.4015255	5
ACCGTCT	110	4.9775736E-7	11.226057	8
TAATACT	290	0.0	10.812577	4
TCTAGAC	220	0.0	10.796899	3
CTAGACA	205	0.0	10.660737	4
GCGTTTA	100	2.4042165E-5	10.44811	13
AGGCGTG	175	2.3646862E-10	10.313169	7
GTATAAT	260	0.0	10.242805	1
ATAACAC	225	0.0	10.134689	3
CCTACAC	265	0.0	10.03908	3
GTCCTAA	390	0.0	9.998928	1
CGTTTAT	105	4.1141568E-5	9.950581	14
GTATTAG	220	1.8189894E-12	9.943501	1
GTGTCGG	115	1.012416E-5	9.9247055	1
TAGGACC	375	0.0	9.88204	4
TCCTACA	985	0.0	9.645961	2
GTACTAG	200	2.582965E-10	9.511176	1
TAATACC	180	4.1673047E-9	9.501962	4
TAAACAG	390	0.0	9.501271	5
>>END_MODULE