FastQCFastQC Report
Thu 26 May 2016
SRR1513026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066061
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44870.21717654996633692No Hit
GTACATGGGAAGCAGTGGTATCAAC38860.1880873798014676No Hit
CCCATGTACTCTGCGTTGATACCAC37000.17908474144761455No Hit
TATCAACGCAGAGTACTTTTTTTTT30890.149511558468022No Hit
GGTATCAACGCAGAGTACTTTTTTT25830.1250205100430239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG903.7067366E-813.7035329
TAGCCCG657.888108E-411.7163755
CGTGCGC855.413685E-511.15827610
GTAGGAC7600.010.8973863
GGCGTGC1252.2833774E-710.6255078
TGGCGAG6300.010.54500718
TAGACAG3850.010.3849695
AAACGCC750.002678848810.11928719
ACCGCTG856.678048E-410.0451228
AGGACGT6550.010.0282345
TGTACCG951.6106415E-410.0205845
CCATACT2201.8189894E-129.9522634
ATGGCGA7050.09.82895317
TCGAACT1651.045737E-89.7743119
TAGGACC3900.09.7636474
CCTACAC2650.09.6991693
TCTAGAC2850.09.6865653
GGACGTG6800.09.6595496
TAGGACG6900.09.657524
TAGGACT2500.09.5195564
Produced by FastQC (version 0.11.5)