FastQCFastQC Report
Thu 26 May 2016
SRR1513025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230273
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC96040.4306199285917016No Hit
CCCATGTACTCTGCGTTGATACCAC80720.3619287862965655No Hit
GAGTACATGGGAAGCAGTGGTATCA37320.1673337748338432No Hit
GTATCAACGCAGAGTACTTTTTTTT28840.1293115237461961No Hit
GTATCAACGCAGAGTACATGGGAAG27590.12370682871558772No Hit
GCGTTGATACCACTGCTTCCCATGT26200.11747440784155123No Hit
CATGTACTCTGCGTTGATACCACTG25090.11249743865437102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACC604.0882142E-412.6685222
GCGTTTA1501.8189894E-1212.66482713
TTACGCT658.018993E-411.6934944
CGTGCGG658.0356066E-411.6906110
GAATCGC700.001493371110.855816
TAACACC1451.4419129E-810.4838244
GTAAGAC1659.276846E-1010.3646883
AACCCGA856.6027866E-410.0575896
TAGGACC2107.2759576E-129.9533914
TAGGACT2900.09.8285854
CGTTTAT1951.6370905E-109.74217514
GGTCTAC2350.09.7153161
TAAGACT2450.09.6948624
GTAGGAC2650.09.6802283
CTTAGAC2750.09.673713
GTCCTAC3050.09.6688641
AGGACTG5550.09.5863455
AAGTGCG800.004522169.4986217
GCTACAC3050.09.3452113
ATAATAC2054.2200554E-109.2692343
Produced by FastQC (version 0.11.5)