##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513025_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2230273 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.227898109334596 31.0 30.0 34.0 24.0 34.0 2 28.939112835065483 31.0 30.0 34.0 16.0 34.0 3 29.30826943607352 31.0 30.0 34.0 23.0 34.0 4 32.156008703867194 35.0 32.0 37.0 19.0 37.0 5 32.40055410256951 35.0 33.0 37.0 22.0 37.0 6 32.854777868000916 37.0 33.0 37.0 26.0 37.0 7 32.84562248657451 37.0 35.0 37.0 26.0 37.0 8 32.822799271658674 37.0 35.0 37.0 26.0 37.0 9 34.18989155139304 39.0 35.0 39.0 25.0 39.0 10 33.69999950678684 39.0 34.0 39.0 19.0 39.0 11 34.05764406420201 39.0 34.0 39.0 25.0 39.0 12 33.896462899384964 39.0 34.0 39.0 23.0 39.0 13 33.973981212165505 39.0 34.0 39.0 25.0 39.0 14 34.26391387960128 39.0 33.0 41.0 17.0 41.0 15 34.80595245514787 40.0 34.0 41.0 22.0 41.0 16 34.38273296587458 39.0 34.0 41.0 17.0 41.0 17 33.911654761547126 39.0 33.0 41.0 11.0 41.0 18 33.30430310549426 39.0 32.0 41.0 10.0 41.0 19 34.2145867344491 39.0 33.0 41.0 18.0 41.0 20 34.47558617263447 39.0 34.0 41.0 18.0 41.0 21 34.50437816357011 39.0 34.0 41.0 18.0 41.0 22 34.08205318362371 39.0 34.0 41.0 10.0 41.0 23 32.37358655196023 39.0 31.0 41.0 2.0 41.0 24 33.46069472212594 39.0 33.0 41.0 2.0 41.0 25 33.20649669345412 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7171875559227558 1101 2 1.0388695019369614 1101 3 0.8147418291046442 1101 4 0.848377177359076 1101 5 0.9173956368457041 1101 6 0.7077598801595784 1101 7 0.7711738742458891 1101 8 0.6816484410621726 1101 9 0.8525407315651705 1101 10 1.1480743872418273 1101 11 0.8839813934246408 1101 12 0.8819793455296718 1101 13 0.8821558141021697 1101 14 -0.2578203605910332 1101 15 0.47968231424292895 1101 16 1.1897884653791166 1101 17 1.7974624775889794 1101 18 -4.246946497298545 1101 19 -0.6844928079569712 1101 20 0.35798091181573 1101 21 0.6919715003596707 1101 22 1.2052094678892402 1101 23 3.038589681198559 1101 24 1.9477044432284174 1101 25 2.1335236082088542 1102 1 0.9503163902785836 1102 2 1.2582676906603005 1102 3 0.9970380604806302 1102 4 1.5203023891615857 1102 5 1.369338377132003 1102 6 0.9259895032971741 1102 7 0.8316471433081176 1102 8 0.8195391801799019 1102 9 0.9311267672064218 1102 10 1.4010688365552966 1102 11 1.0285913846603947 1102 12 1.0832647676739882 1102 13 1.0679577422353859 1102 14 1.8438448453686505 1102 15 1.398174864637319 1102 16 1.5824265021889303 1102 17 1.3300362432915733 1102 18 1.6946252234142847 1102 19 1.5182533211695315 1102 20 1.351845942490577 1102 21 1.3212440682825388 1102 22 1.6802313784939713 1102 23 3.2769770606902355 1102 24 2.281329917958189 1102 25 2.5033746160966714 1103 1 0.9522512725788239 1103 2 1.2661791649463368 1103 3 1.0770816720858178 1103 4 1.4766045939985375 1103 5 1.44565338087574 1103 6 0.9682852110973315 1103 7 0.9537585071909689 1103 8 0.826724084288557 1103 9 0.9553588480193937 1103 10 1.295430716338629 1103 11 1.0732370955959212 1103 12 1.2185716339882973 1103 13 0.976158548698713 1103 14 1.7865662585850401 1103 15 1.4821409463167114 1103 16 1.6150965901888128 1103 17 2.154582417417764 1103 18 2.0473895689788506 1103 19 1.5625882785446308 1103 20 1.5156679486261524 1103 21 1.4680827356968251 1103 22 1.7864536075580517 1103 23 3.264702686430752 1103 24 2.2099540954897066 1103 25 2.3220832676306102 1104 1 0.7106681449592678 1104 2 1.031033485389358 1104 3 0.7895146660510441 1104 4 1.406862737594139 1104 5 1.065652686340897 1104 6 0.7170064293463554 1104 7 0.6574865189680423 1104 8 0.6666709715683439 1104 9 0.7739716490295763 1104 10 1.1814248277999582 1104 11 0.761700697258199 1104 12 0.8972322601795213 1104 13 0.7089221307813887 1104 14 1.6893378365437854 1104 15 1.093004064666495 1104 16 1.5420686067166471 1104 17 1.7885290471263318 1104 18 2.490787160164473 1104 19 1.586711759860421 1104 20 0.9241416053381712 1104 21 1.1165233304559834 1104 22 1.4053413082533197 1104 23 2.9522017920862567 1104 24 1.8908868802219416 1104 25 1.953969327618175 1105 1 0.7030002001595967 1105 2 1.0134583033535272 1105 3 0.6947709667006912 1105 4 0.901767867361329 1105 5 1.007001654193033 1105 6 0.6291151610937149 1105 7 0.4897345484418949 1105 8 0.4005908126634736 1105 9 0.5521022643251925 1105 10 1.132155360161974 1105 11 0.6759004307102998 1105 12 0.5964291182589747 1105 13 0.582933369478269 1105 14 1.32041319178456 1105 15 0.7323792641081468 1105 16 1.0868439607628986 1105 17 1.5411906865836116 1105 18 1.5553092425144612 1105 19 1.032267815975409 1105 20 0.8873475007906464 1105 21 0.7706310178543418 1105 22 1.2494016586562964 1105 23 2.7053374840783846 1105 24 1.7245295934133367 1105 25 1.6354865687621114 1106 1 -0.5230504562419576 1106 2 -0.02448182716793923 1106 3 -0.2382129526685013 1106 4 -2.903885360837375 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-1.7608294256814219 2215 16 -2.4417421021258647 2215 17 -7.049835788257781 2215 18 -3.284956196775415 2215 19 -2.3392328379743788 2215 20 -2.081782257617199 2215 21 -2.134212975217835 2215 22 -1.6597786528096208 2215 23 -6.509379840468913 2215 24 -3.644361455455414 2215 25 -3.2961910447142557 2216 1 -0.9780888253383715 2216 2 -0.4795649506370374 2216 3 -0.6971751873173524 2216 4 -2.994760989313221 2216 5 -1.141742656974582 2216 6 -0.9724613447586918 2216 7 -1.1090169956627527 2216 8 -0.8907897609613542 2216 9 -0.9372020447411487 2216 10 -0.6211539731494042 2216 11 -1.1701942733292867 2216 12 -2.11435916994985 2216 13 -1.2947101121949558 2216 14 -7.059208429561583 2216 15 -3.264548168559873 2216 16 -1.8729678025408987 2216 17 -0.8517687058653536 2216 18 0.04412665142943695 2216 19 -0.5178913282407294 2216 20 -1.0289809427636314 2216 21 -1.2842678758787187 2216 22 -0.9145039078042032 2216 23 0.24482057801914436 2216 24 -0.6174635844961358 2216 25 -0.36498606152974133 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 106329.0 3 3.0 4 946.0 5 5515.0 6 6477.0 7 5979.0 8 5609.0 9 5268.0 10 5007.0 11 4810.0 12 4907.0 13 4825.0 14 4905.0 15 5027.0 16 5052.0 17 5409.0 18 6067.0 19 7172.0 20 8826.0 21 11153.0 22 13858.0 23 16637.0 24 19833.0 25 22770.0 26 26256.0 27 30052.0 28 33803.0 29 40878.0 30 47780.0 31 54838.0 32 70314.0 33 92593.0 34 120210.0 35 156770.0 36 220163.0 37 304246.0 38 749986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.50948041226562 17.14437960614888 11.686156313114648 25.659983668470854 2 14.879073408261542 19.44559705191668 39.93773676738088 25.73759277244089 3 19.376262319317057 24.11224443570318 28.489495754032134 28.02199749094763 4 11.206067181594289 15.864635721074341 37.84587962965039 35.083417467680974 5 13.049667911773255 37.33202722728757 35.589595645356326 14.028709215582849 6 33.67132863996728 35.161228694411285 17.51536226788423 13.65208039773721 7 29.149286643576517 30.88041991002825 21.272161631636045 18.69813181475919 8 26.91286012655304 34.50550446084 19.87866730470903 18.702968107897927 9 27.32505570445959 14.717862742776362 18.658078671560766 39.299002881203286 10 14.769142027732201 28.20500514472972 34.05389452003305 22.971958307505027 11 35.55159211902986 22.34455856889346 22.439633209213092 19.664216102863584 12 23.75702748567595 24.406632654891705 30.279718147147566 21.55662171228478 13 29.437872210662263 20.595043768386308 24.75277139987085 25.214312621080577 14 21.953584365297655 20.423318768558204 27.32547944689433 30.29761741924981 15 25.232860653153445 27.4177077694665 22.751839093501697 24.597592483878362 16 23.701341073405864 26.91031092291126 25.886039929481864 23.502308074201007 17 23.284676705366522 26.731155461110728 26.869025375419685 23.115142458103062 18 23.105076196084113 26.173728237778604 27.89394211869108 22.827253447446203 19 24.21111837227525 26.54458342322575 26.706681312589232 22.537616891909774 20 24.5760192020752 26.03232008481634 26.384510613030375 23.007150100078082 21 24.283312866596045 25.814147075259886 26.59230169615282 23.31023836199125 22 24.07709176311435 25.791732209826023 25.940244374501837 24.190931652557786 23 22.454214294235893 25.659460375865727 28.054301483746602 23.832023846151777 24 23.7174469734247 26.747902239211342 25.837612883460626 23.697037903903333 25 23.817451728169868 25.467603570664505 26.12641745450892 24.588527246656707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 359.0 1 359.0 2 494.5 3 630.0 4 630.0 5 630.0 6 1210.0 7 1790.0 8 1790.0 9 1790.0 10 2629.5 11 3469.0 12 3469.0 13 3469.0 14 5718.5 15 7968.0 16 7968.0 17 7968.0 18 14232.0 19 20496.0 20 20496.0 21 20496.0 22 30897.5 23 41299.0 24 41299.0 25 41299.0 26 57688.5 27 74078.0 28 74078.0 29 74078.0 30 95024.5 31 115971.0 32 115971.0 33 115971.0 34 138882.5 35 161794.0 36 161794.0 37 161794.0 38 186302.0 39 210810.0 40 210810.0 41 210810.0 42 235576.5 43 260343.0 44 260343.0 45 260343.0 46 289441.0 47 318539.0 48 318539.0 49 318539.0 50 312945.0 51 307351.0 52 307351.0 53 307351.0 54 280509.5 55 253668.0 56 253668.0 57 253668.0 58 222582.0 59 191496.0 60 191496.0 61 191496.0 62 159324.0 63 127152.0 64 127152.0 65 127152.0 66 100190.5 67 73229.0 68 73229.0 69 73229.0 70 54516.0 71 35803.0 72 35803.0 73 35803.0 74 25461.0 75 15119.0 76 15119.0 77 15119.0 78 10542.0 79 5965.0 80 5965.0 81 5965.0 82 4028.5 83 2092.0 84 2092.0 85 2092.0 86 1369.5 87 647.0 88 647.0 89 647.0 90 409.5 91 172.0 92 172.0 93 172.0 94 97.5 95 23.0 96 23.0 97 23.0 98 16.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12016466145624324 2 0.04609301193172316 3 0.033448819942670696 4 0.03156564241238628 5 0.033403982382425826 6 0.020580440152393898 7 0.020714952833128502 8 0.02403293229124865 9 0.029099576598918608 10 0.036183911117607576 11 0.01999755186921063 12 0.020490765031904168 13 0.01582765876643801 14 0.020804627953618236 15 0.022687805483902642 16 0.022060079640474507 17 0.028471850755490472 18 0.019459501146272228 19 0.033897195545119364 20 0.02304650596586158 21 0.028651200996469936 22 0.03703582476226005 23 0.029009901478428874 24 0.03909835253352392 25 0.029592789761612143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2230273.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.24215218066418 #Duplication Level Percentage of deduplicated Percentage of total 1 80.51155348487087 55.74793238701111 2 12.309776909710884 17.047108921844536 3 3.6935560692129705 7.6724931429678085 4 1.4850331243304644 4.113075583528688 5 0.7259885165848279 2.5134503673390642 6 0.37720589994435233 1.5671128996434736 7 0.2274435821829484 1.102407819101898 8 0.1514868914533666 0.8391422713111814 9 0.10486735702522797 0.6535117344532388 >10 0.3892826160715098 4.327144473866137 >50 0.014734118487694587 0.7120808565165276 >100 0.00644512639755741 0.8710748583959401 >500 0.0017070974227606818 0.8383782148101303 >1k 7.87891118196675E-4 1.1914872433020176 >5k 1.313151863661125E-4 0.8035992259083663 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 9604 0.4306199285917016 No Hit CCCATGTACTCTGCGTTGATACCAC 8072 0.3619287862965655 No Hit GAGTACATGGGAAGCAGTGGTATCA 3732 0.1673337748338432 No Hit GTATCAACGCAGAGTACTTTTTTTT 2884 0.1293115237461961 No Hit GTATCAACGCAGAGTACATGGGAAG 2759 0.12370682871558772 No Hit GCGTTGATACCACTGCTTCCCATGT 2620 0.11747440784155123 No Hit CATGTACTCTGCGTTGATACCACTG 2509 0.11249743865437102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.967512048973376E-5 0.0 0.0 0.0 6 4.483756024486688E-5 8.967512048973376E-5 0.0 0.0 0.0 7 4.483756024486688E-5 8.967512048973376E-5 0.0 0.0 0.0 8 4.483756024486688E-5 8.967512048973376E-5 0.0 0.0 0.0 9 4.483756024486688E-5 8.967512048973376E-5 0.0 0.0 0.0 10 4.483756024486688E-5 8.967512048973376E-5 0.0 0.0 0.0 11 4.483756024486688E-5 8.967512048973376E-5 0.0 0.0 0.0 12 4.483756024486688E-5 8.967512048973376E-5 0.0 4.483756024486688E-5 0.0 13 4.483756024486688E-5 8.967512048973376E-5 0.0 4.483756024486688E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACC 60 4.0882142E-4 12.668522 2 GCGTTTA 150 1.8189894E-12 12.664827 13 TTACGCT 65 8.018993E-4 11.693494 4 CGTGCGG 65 8.0356066E-4 11.69061 10 GAATCGC 70 0.0014933711 10.85581 6 TAACACC 145 1.4419129E-8 10.483824 4 GTAAGAC 165 9.276846E-10 10.364688 3 AACCCGA 85 6.6027866E-4 10.057589 6 TAGGACC 210 7.2759576E-12 9.953391 4 TAGGACT 290 0.0 9.828585 4 CGTTTAT 195 1.6370905E-10 9.742175 14 GGTCTAC 235 0.0 9.715316 1 TAAGACT 245 0.0 9.694862 4 GTAGGAC 265 0.0 9.680228 3 CTTAGAC 275 0.0 9.67371 3 GTCCTAC 305 0.0 9.668864 1 AGGACTG 555 0.0 9.586345 5 AAGTGCG 80 0.00452216 9.498621 7 GCTACAC 305 0.0 9.345211 3 ATAATAC 205 4.2200554E-10 9.269234 3 >>END_MODULE