FastQCFastQC Report
Thu 26 May 2016
SRR1513025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230273
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC110120.49375121341647416No Hit
CCCATGTACTCTGCGTTGATACCAC105480.47294658546285595No Hit
GTATCAACGCAGAGTACTTTTTTTT47250.21185747215699602No Hit
GAGTACATGGGAAGCAGTGGTATCA40030.17948475366020214No Hit
TATCAACGCAGAGTACTTTTTTTTT32760.1468878473621839No Hit
CATGTACTCTGCGTTGATACCACTG30280.1357681324214569No Hit
GGTATCAACGCAGAGTACTTTTTTT28770.12899766082448202No Hit
GTATCAACGCAGAGTACATGGGAAG28140.12617289452905542No Hit
GCGTTGATACCACTGCTTCCCATGT27210.12200300142628279No Hit
TATCAACGCAGAGTACATGGGAAGC25660.11505317958832842No Hit
ACGCAGAGTACATGGGAAGCAGTGG23300.10447151537053984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA500.001462669913.3444351
TAAGACT2850.011.0263154
CTAAGAC2600.010.6215033
CTAATAC1803.45608E-1110.5808083
GTGTACG1053.958586E-59.9856321
GTCCTAC2201.8189894E-129.9650011
TCTATAC3450.09.9367583
GTCTTAC2251.8189894E-129.7435561
CCATACG800.00440723649.5283042
CGTAAGT800.00440723649.5283042
TTATACT2800.09.5227274
ATTATAC2106.366463E-119.5227273
GTTGGAC1606.397204E-89.5227273
GTCCGCG800.0045728919.48578311
TAATACT2650.09.3430544
GTACTAA1356.5650966E-69.1787121
CCTAGTA1252.6597278E-59.1471722
GCATAAG1151.0712218E-49.1173161
TTACACA3450.09.1086954
GCGTGCG1252.8029935E-59.1065559
Produced by FastQC (version 0.11.5)