FastQCFastQC Report
Thu 26 May 2016
SRR1513024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246921
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC45030.20040757997277164No Hit
CCCATGTACTCTGCGTTGATACCAC38790.1726362431077906No Hit
GTATCAACGCAGAGTACTTTTTTTT32310.1437967779018488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC1501.4551915E-1112.03169810
TATTAGG2050.011.1241542
CAGCGTA600.00588298811.0818279
GTGCGCC1803.6379788E-1211.08158111
TAGGACC5550.010.9561494
GTATACG700.001477531910.86971
GTAGGAC10100.010.7237023
TGTAGGA11600.010.6486612
AGGACGT7350.010.5993225
GTATTAT2900.010.4948841
GCGTGCG1451.4473699E-810.4813349
GGACGTG7250.010.3510086
GCGCCAC1752.3646862E-1010.31288413
GTCTAAC1303.7826794E-710.2426021
AAGGCGT2053.6379788E-1210.1944176
GTCTAGG1909.822543E-1110.0115661
GTCCTAG3900.09.9987321
CTAGGAC3250.09.9393233
TAGGACG8150.09.9090564
GTCCTAA5200.09.8767951
Produced by FastQC (version 0.11.5)