##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513024_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2246921
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.489682992860008	31.0	30.0	34.0	25.0	34.0
2	29.226850877267157	31.0	30.0	34.0	20.0	34.0
3	29.59186371038412	31.0	30.0	34.0	25.0	34.0
4	32.47154305825617	35.0	33.0	37.0	19.0	37.0
5	32.72769892666453	37.0	33.0	37.0	25.0	37.0
6	33.180214168633434	37.0	35.0	37.0	27.0	37.0
7	33.17363227278574	37.0	35.0	37.0	27.0	37.0
8	33.14825621372536	37.0	35.0	37.0	27.0	37.0
9	34.55649664585448	39.0	35.0	39.0	27.0	39.0
10	34.0842610843906	39.0	34.0	39.0	24.0	39.0
11	34.43961625709137	39.0	35.0	39.0	27.0	39.0
12	34.28597845674147	39.0	35.0	39.0	26.0	39.0
13	34.36262245090059	39.0	35.0	39.0	27.0	39.0
14	34.71830473790578	40.0	34.0	41.0	17.0	41.0
15	35.25372320611183	40.0	35.0	41.0	25.0	41.0
16	34.844590886818004	40.0	34.0	41.0	19.0	41.0
17	34.389576669584734	39.0	34.0	41.0	17.0	41.0
18	33.8094819533041	39.0	33.0	41.0	11.0	41.0
19	34.713869780023416	39.0	34.0	41.0	19.0	41.0
20	34.966613423435895	40.0	35.0	41.0	23.0	41.0
21	34.99062628370112	40.0	35.0	41.0	22.0	41.0
22	34.585851928038416	40.0	34.0	41.0	18.0	41.0
23	32.90239309704258	39.0	32.0	41.0	9.0	41.0
24	34.01360083420823	39.0	34.0	41.0	10.0	41.0
25	33.756208607245206	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.6609125895693495
1101	2	1.0710529795312773
1101	3	0.8467981976092176
1101	4	0.797965637910707
1101	5	0.990420474436057
1101	6	0.6144149311854434
1101	7	0.6306771726160179
1101	8	0.7034706408359241
1101	9	0.7879062041337761
1101	10	1.1169228027424225
1101	11	0.7462427678835866
1101	12	0.8068928375198325
1101	13	0.6938093155894904
1101	14	-0.24147964606967776
1101	15	0.39018820846970215
1101	16	1.0025638202304918
1101	17	1.6572307744294932
1101	18	-4.387991366233578
1101	19	-0.7155578576479726
1101	20	0.2049183193307229
1101	21	0.4016754934273692
1101	22	1.0040657563983615
1101	23	2.787223285989242
1101	24	1.6332743969738246
1101	25	1.8664496816998764
1102	1	0.7568591471204584
1102	2	1.0401865245301138
1102	3	0.7823078671239614
1102	4	1.4018917170648137
1102	5	1.1265678532383276
1102	6	0.8018954867352761
1102	7	0.7305325368118574
1102	8	0.7828326705721054
1102	9	0.8139243378648899
1102	10	1.148432426929702
1102	11	0.8249195631772253
1102	12	0.894015821220485
1102	13	0.7439825214290465
1102	14	1.6457068304139568
1102	15	1.0476867095730142
1102	16	1.3436587943079914
1102	17	1.0711219926461908
1102	18	1.5469293896911012
1102	19	1.2369918463156395
1102	20	1.1684056767466728
1102	21	1.1642390978120432
1102	22	1.5683390644994049
1102	23	3.1361332979323393
1102	24	1.8795820976285356
1102	25	2.1496721344511514
1103	1	0.9005691831895781
1103	2	1.1749479253815238
1103	3	0.9693669022144107
1103	4	1.3272132440717002
1103	5	1.2389214413033542
1103	6	0.9165399655312712
1103	7	0.797484851564235
1103	8	0.8628803592779448
1103	9	1.032533480678559
1103	10	1.2402650011861027
1103	11	0.8693527108047192
1103	12	1.0966165675020463
1103	13	0.8564322477117798
1103	14	1.7419902929114102
1103	15	1.2390432613233884
1103	16	1.4794937962273025
1103	17	1.8889009923245723
1103	18	1.8221547784802752
1103	19	1.2496914282181422
1103	20	1.1836871576902084
1103	21	1.1737711329336165
1103	22	1.4540971212624498
1103	23	3.0426131175521434
1103	24	1.944136490073035
1103	25	2.0326031051359124
1104	1	0.5793650391390166
1104	2	1.003390919227229
1104	3	0.7729406705915842
1104	4	1.2794186746517227
1104	5	1.1274801549096765
1104	6	0.704980718982668
1104	7	0.6099920225232935
1104	8	0.5842874966310845
1104	9	0.8227191957886646
1104	10	1.2133451360171037
1104	11	0.7874389727747086
1104	12	0.8684083058783045
1104	13	0.6935445491738079
1104	14	1.5423270309651826
1104	15	1.0280099982569695
1104	16	1.3151939703670408
1104	17	1.7915512769564899
1104	18	2.4191322828846893
1104	19	1.5306770998781687
1104	20	0.9816775918854148
1104	21	1.0725967929164426
1104	22	1.3569666929737423
1104	23	2.831840724641296
1104	24	1.7723831099629876
1104	25	1.9208210072442569
1105	1	0.483591181050425
1105	2	0.8319965741038224
1105	3	0.5304082410250679
1105	4	0.7304441160153985
1105	5	0.6964333463085666
1105	6	0.4079797728711583
1105	7	0.33891546190438504
1105	8	0.31127054369481755
1105	9	0.5490647470713625
1105	10	0.9772660639505162
1105	11	0.5348886574484979
1105	12	0.618211974452251
1105	13	0.5033432480047253
1105	14	1.1978949370556435
1105	15	0.5785189982755696
1105	16	0.9790646029063623
1105	17	1.4427616884352688
1105	18	1.4258826290392506
1105	19	0.9999474113774127
1105	20	0.6961216716458054
1105	21	0.5611225332619867
1105	22	1.0140215355498228
1105	23	2.57070365009249
1105	24	1.6283532463252968
1105	25	1.6277443642392342
1106	1	-0.6436820344820937
1106	2	-0.17135618383161955
1106	3	-0.41583752942447205
1106	4	-2.6444021431821945
1106	5	-0.7017170645620467
1106	6	-0.6210174879914021
1106	7	-0.6019346106021928
1106	8	-0.47914046119266374
1106	9	-0.3812973830108106
1106	10	-0.35622861890033874
1106	11	-0.4223002356177261
1106	12	-0.32811503022039545
1106	13	-0.2947031024974507
1106	14	0.2651937984338062
1106	15	-0.28975467377985353
1106	16	-1.1676712583106692
1106	17	-5.928106107901808
1106	18	-7.002917252801883
1106	19	-2.943259796313658
1106	20	-1.6603273857686602
1106	21	-1.2393917263652767
1106	22	-1.6720699570115372
1106	23	-5.284652437774742
1106	24	-2.414755943212274
1106	25	-0.936636107322073
1107	1	0.47043728537893514
1107	2	0.7690444716132347
1107	3	0.4576719574502093
1107	4	0.6596340474878986
1107	5	0.7601286399098228
1107	6	0.3382465814148219
1107	7	0.2030118471715241
1107	8	0.2732994782542164
1107	9	0.28518355786123095
1107	10	0.6482873337605213
1107	11	0.3824269469545314
1107	12	0.3681072135289085
1107	13	0.34613150604172205
1107	14	1.0834013570659096
1107	15	0.5921254448307067
1107	16	-5.818763283293759
1107	17	-1.0602216743887425
1107	18	0.524377789329165
1107	19	0.44249807000974073
1107	20	0.3724987111820113
1107	21	0.4420438489222178
1107	22	0.5370836948933899
1107	23	2.4214958057249945
1107	24	1.4454689636922282
1107	25	1.2058896540250714
1108	1	0.5064927510387278
1108	2	0.8258976154185369
1108	3	0.6208877771173071
1108	4	1.0207286457542821
1108	5	0.7843806485495364
1108	6	0.5657598539344377
1108	7	0.5195371568663489
1108	8	0.5967016114167123
1108	9	0.7658581827739184
1108	10	1.1538160448043158
1108	11	0.6662002780649132
1108	12	0.7189724892928737
1108	13	0.6367596254909103
1108	14	1.1902790230532148
1108	15	0.8549455557095271
1108	16	1.1378541656442849
1108	17	1.2185944818325467
1108	18	1.0451164100888874
1108	19	0.9951176336720664
1108	20	0.8816024598596996
1108	21	1.0163591842539432
1108	22	1.3176575576984604
1108	23	2.973019445772735
1108	24	1.724362323876008
1108	25	1.9038047257777677
1109	1	0.3205108908823533
1109	2	0.5844584310849079
1109	3	0.49130655608495744
1109	4	0.4332372858730196
1109	5	0.252286890778322
1109	6	0.14175667397912406
1109	7	0.24187409114662017
1109	8	0.1542824956557638
1109	9	0.21126584952087057
1109	10	0.8279430746203147
1109	11	0.3680554804443972
1109	12	0.4287800275884308
1109	13	0.2470687312459674
1109	14	-0.7386619969996957
1109	15	0.12180675966724408
1109	16	-0.057054674983952225
1109	17	0.8588693795707414
1109	18	1.5700772970301173
1109	19	0.7272524136500991
1109	20	0.6539052252851221
1109	21	0.5293154461616396
1109	22	0.4163732395384514
1109	23	-4.375327623636053
1109	24	-1.4658426696720497
1109	25	-0.16742558796868678
1110	1	0.5273674586604216
1110	2	0.9686343531002706
1110	3	0.6931102551805424
1110	4	1.1753811784843435
1110	5	0.8132932411080134
1110	6	0.473813473093351
1110	7	0.34124971181620367
1110	8	0.4824164727732736
1110	9	0.4280046184929063
1110	10	0.8110580083584864
1110	11	0.576584289025142
1110	12	0.5638786308202839
1110	13	0.36420644094192056
1110	14	1.2763624873451604
1110	15	0.7083361846370906
1110	16	1.0725202067796857
1110	17	1.4554122737839137
1110	18	1.9345791823436969
1110	19	1.169429789126177
1110	20	0.7978834462035422
1110	21	0.7496602816199953
1110	22	1.006533462656087
1110	23	2.385929087205163
1110	24	1.4649457756957105
1110	25	1.7003722668450507
1111	1	0.25448184390398154
1111	2	0.5969869041675508
1111	3	0.32470735067082757
1111	4	0.8670011043446095
1111	5	0.5899187411042917
1111	6	0.1336673413451095
1111	7	0.11122318190829361
1111	8	-0.10157079734017316
1111	9	0.05241772031958192
1111	10	0.3665150750966575
1111	11	0.05592398357738659
1111	12	0.22714277782850445
1111	13	0.005892536390000203
1111	14	-0.672755520466076
1111	15	-0.18115222389121755
1111	16	0.25545118432671643
1111	17	0.7612067061034722
1111	18	1.4206065986509628
1111	19	0.49216388421672264
1111	20	0.3429253205036744
1111	21	0.2785784398956608
1111	22	0.5561963453658336
1111	23	0.4732462518197025
1111	24	0.2942524475268371
1111	25	0.11182947510916819
1112	1	0.5370404635581458
1112	2	0.8994499306183421
1112	3	0.6372458256839657
1112	4	1.1750746010038498
1112	5	0.9482731366399406
1112	6	0.36416019687925427
1112	7	0.2970887758623988
1112	8	0.2629715064059823
1112	9	0.2577116318833603
1112	10	0.7438073100257157
1112	11	0.44301201941232193
1112	12	0.3809815206027096
1112	13	0.22163476005807325
1112	14	0.857998214425443
1112	15	0.4022397082426181
1112	16	0.7723470327789528
1112	17	1.1422394630414914
1112	18	1.1945023970544
1112	19	0.6787483040260369
1112	20	0.6005427474949911
1112	21	0.6777169749673604
1112	22	0.6689706753375617
1112	23	2.193091225669207
1112	24	1.1921685407176952
1112	25	1.3566419172402462
1113	1	0.34068304538990546
1113	2	0.6946193340110369
1113	3	0.4571124970824272
1113	4	0.5302860813663912
1113	5	0.594318796401275
1113	6	0.19692113735430183
1113	7	0.12430857904031711
1113	8	0.12577265684927852
1113	9	0.21695467845965055
1113	10	0.5022619727464743
1113	11	0.27012821171657464
1113	12	0.27684040305567237
1113	13	0.2489655803969697
1113	14	0.8662904017181035
1113	15	0.4184264577774073
1113	16	0.7968176375331737
1113	17	1.2281341116903022
1113	18	1.8216263813901037
1113	19	0.9127575896587601
1113	20	0.5898287813972729
1113	21	0.44138805557553695
1113	22	0.7416076839381063
1113	23	0.5702254707988033
1113	24	0.47284792871148795
1113	25	0.9357181526244034
1114	1	-0.17459952412847812
1114	2	-2.845295336427988
1114	3	-0.9192733370065049
1114	4	0.21514309415453425
1114	5	0.4640881281744953
1114	6	0.26436798508919424
1114	7	0.19550804629490415
1114	8	0.19321777391405703
1114	9	0.24124403145238915
1114	10	0.4031477748556398
1114	11	0.1326710928308259
1114	12	0.23316483597903215
1114	13	0.08900508482334857
1114	14	0.8126380481948772
1114	15	0.2221288878639811
1114	16	0.6597435234669646
1114	17	1.0473056474342854
1114	18	1.207062039966516
1114	19	0.5516334818003941
1114	20	0.4039797184046421
1114	21	0.29145408440248133
1114	22	0.5559939168336356
1114	23	2.1492086644871264
1114	24	1.1235699567558655
1114	25	1.1612740551500522
1115	1	0.2484321532600866
1115	2	0.6287661242906459
1115	3	0.3540694912815532
1115	4	0.6348302045685301
1115	5	0.5343613979039645
1115	6	0.059111602814923
1115	7	-0.010583867333068042
1115	8	0.001693981653801302
1115	9	0.02154796865251285
1115	10	0.21635136024919177
1115	11	0.2560091489793024
1115	12	0.08691089156521059
1115	13	-0.1097543808449899
1115	14	0.8667846675594078
1115	15	0.22893625249613336
1115	16	0.6708275390189584
1115	17	1.21206661503183
1115	18	1.6249116916743134
1115	19	0.8797997594205356
1115	20	0.3500639374199537
1115	21	0.46263036131975355
1115	22	0.8425950066571417
1115	23	2.4356167971008134
1115	24	1.2431661375644651
1115	25	1.0271845534872028
1116	1	0.0827613461822665
1116	2	0.11392618509162489
1116	3	0.14727418904756462
1116	4	0.725387535635285
1116	5	0.265829511755193
1116	6	0.06911011171102643
1116	7	0.010805548533554088
1116	8	-0.02153178311910864
1116	9	0.05215225634617582
1116	10	0.3797236888914952
1116	11	-0.03909601140451002
1116	12	0.10456804058208036
1116	13	-0.08707623054998237
1116	14	0.6389756174977492
1116	15	0.03766187698509782
1116	16	0.493425387547596
1116	17	0.8222339153863487
1116	18	1.3447094574222689
1116	19	0.5367334113067486
1116	20	0.25390103225378624
1116	21	0.14396613500549194
1116	22	0.5154154878893209
1116	23	1.9899974312293622
1116	24	0.7818502928390885
1116	25	1.1016017553424788
1201	1	1.0652761929660848
1201	2	1.4805542132892207
1201	3	1.2444431749808054
1201	4	1.812833018015077
1201	5	1.5561286819454594
1201	6	1.1420496557880426
1201	7	1.1132203022400162
1201	8	1.051068229860789
1201	9	1.220816485311552
1201	10	1.5384088417790664
1201	11	1.2350889455523486
1201	12	1.406033618799512
1201	13	1.274929722129258
1201	14	2.1581433925216373
1201	15	1.562187145475903
1201	16	1.9716792135364685
1201	17	2.4651917052549663
1201	18	2.877305346409649
1201	19	2.0258462353626
1201	20	1.7034015116063017
1201	21	1.5747818886569078
1201	22	1.9105062116171467
1201	23	3.485313495823249
1201	24	2.35732231335718
1201	25	2.62338120060798
1202	1	1.0870639276647651
1202	2	1.3506452592499159
1202	3	1.0976122679890814
1202	4	1.6408860568511088
1202	5	1.4607607341835802
1202	6	1.0404869245079027
1202	7	0.8812444702658766
1202	8	0.8977667653156374
1202	9	1.0638578870119417
1202	10	1.5533297148955256
1202	11	1.1167087558902935
1202	12	1.1950707110239875
1202	13	1.1281530888773617
1202	14	2.0325322843082603
1202	15	1.46743506369031
1202	16	1.6980583304110937
1202	17	2.138466434696845
1202	18	2.746984229131847
1202	19	1.9127498057288577
1202	20	1.6894006488652948
1202	21	1.6065912341719937
1202	22	1.9612007040308086
1202	23	3.504976801277266
1202	24	2.137587676702246
1202	25	2.2981338321795093
1203	1	1.0143900971540774
1203	2	1.3228750804365283
1203	3	1.0818373972448576
1203	4	1.749419197566624
1203	5	1.527099432636824
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2211	16	-0.6169629306439575
2211	17	-0.028210616589547044
2211	18	-5.972798188992488
2211	19	-1.932994145105532
2211	20	-1.1557247940179707
2211	21	-0.959151882569742
2211	22	-0.5025052992430901
2211	23	-4.792577612468584
2211	24	-2.0671059720310936
2211	25	-1.411723049483676
2212	1	0.2628604143831339
2212	2	0.44847430089799545
2212	3	0.2569069368125696
2212	4	0.803240753987879
2212	5	0.6060126916721913
2212	6	0.06258436126609013
2212	7	-0.08359964282410459
2212	8	-0.12202712412345562
2212	9	-0.27400892686063116
2212	10	-3.9062181117554786
2212	11	-1.1353781285849394
2212	12	-0.3184729736219083
2212	13	-0.18257085256750116
2212	14	0.593846782462947
2212	15	0.19088065824592348
2212	16	0.46915278203676536
2212	17	0.934267806359891
2212	18	1.3983365998179025
2212	19	0.5935712487970193
2212	20	0.3146235808886644
2212	21	0.12955615819622324
2212	22	0.3843429037337671
2212	23	1.6272976883109962
2212	24	0.8012079122094136
2212	25	0.7865916875784578
2213	1	-1.3713722197402731
2213	2	-3.817949929725721
2213	3	-1.670268616009679
2213	4	-3.1767615571882395
2213	5	-1.1507365757957864
2213	6	-0.7909721588625089
2213	7	-0.6900882697797002
2213	8	-0.667530620173082
2213	9	-0.6289734439752053
2213	10	-0.7949932359020409
2213	11	-0.7116807375228618
2213	12	-0.5424314800955727
2213	13	-0.8050696771919732
2213	14	-5.823698922627816
2213	15	-2.437847894118839
2213	16	-1.6424676057026062
2213	17	-0.5358202716122307
2213	18	0.22646456299104756
2213	19	-0.39896863877851274
2213	20	-0.6805021328452625
2213	21	-0.8818335868303961
2213	22	-0.742525547604103
2213	23	-5.640827710406459
2213	24	-2.829527326736123
2213	25	-2.0692468023687134
2214	1	-0.0233587282525356
2214	2	0.1575187079040994
2214	3	0.0326716552646964
2214	4	0.41836253874704
2214	5	0.12905115938665546
2214	6	-0.058985932993145695
2214	7	-0.17032289479108442
2214	8	-0.25930272834366264
2214	9	-0.1949165448190513
2214	10	0.28757397814970176
2214	11	-0.19482470011350728
2214	12	-0.08506188053775077
2214	13	-0.2162990795906694
2214	14	0.3328995328857616
2214	15	0.06267028453031287
2214	16	0.4539043586341407
2214	17	0.6727646924136153
2214	18	1.220300749447432
2214	19	0.3021847012754435
2214	20	0.11833259328947321
2214	21	0.05515918693280497
2214	22	0.5122234812034918
2214	23	1.8860981083943145
2214	24	0.7883682084482047
2214	25	0.6508685787438324
2215	1	-2.1692160592158736
2215	2	-4.248504283322543
2215	3	-2.32417002061354
2215	4	-4.012690200734333
2215	5	-4.746589511285894
2215	6	-2.2445231029883104
2215	7	-1.6397387299508779
2215	8	-2.0004823443956745
2215	9	-1.5732287461476346
2215	10	-2.9929230026402145
2215	11	-1.9432358368455738
2215	12	-1.6931954655247665
2215	13	-1.6577906560262505
2215	14	-0.9546123294289615
2215	15	-1.6262633647086986
2215	16	-2.403289223774337
2215	17	-6.771985982338098
2215	18	-3.0265706641177736
2215	19	-2.2713278331490514
2215	20	-1.9273814387984842
2215	21	-1.841176335336641
2215	22	-1.6657524538850765
2215	23	-6.705294025385115
2215	24	-3.8539203887234343
2215	25	-3.6258292910063687
2216	1	-1.1367623127248692
2216	2	-0.7026646651676103
2216	3	-0.8581066552806433
2216	4	-3.2392428638656625
2216	5	-1.4030473378028532
2216	6	-1.0969281795300745
2216	7	-1.1715274004328577
2216	8	-1.0958091008575934
2216	9	-1.1628383724666662
2216	10	-0.8109939099147994
2216	11	-1.4247496990733524
2216	12	-1.9555454028336214
2216	13	-1.50549401358915
2216	14	-6.88599625298642
2216	15	-3.29766620231441
2216	16	-1.955648362638371
2216	17	-1.0322399838930352
2216	18	-0.276910523488354
2216	19	-0.7062762494518893
2216	20	-1.2806820586066436
2216	21	-1.4490977107191227
2216	22	-0.9103217527178487
2216	23	0.2804681763334713
2216	24	-0.4811989107795185
2216	25	-0.408703120347198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95829.0
3	1.0
4	834.0
5	5016.0
6	5984.0
7	5434.0
8	5007.0
9	4708.0
10	4438.0
11	4594.0
12	4420.0
13	4429.0
14	4479.0
15	4555.0
16	4601.0
17	4822.0
18	5662.0
19	6696.0
20	8245.0
21	10404.0
22	12615.0
23	15284.0
24	18512.0
25	21517.0
26	24859.0
27	28600.0
28	32013.0
29	39044.0
30	46280.0
31	51733.0
32	67068.0
33	89118.0
34	116917.0
35	154244.0
36	220297.0
37	311186.0
38	807476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.910628115102234	18.414049576098826	12.407301552366242	25.268020756432698
2	13.932338918991846	20.18624897642767	42.07477833174742	23.806633772833067
3	18.747754450028918	24.866023866161438	29.399908909140205	26.98631277466944
4	10.538450888009676	16.791892807551232	40.137601098386924	32.53205520605217
5	12.269845509995104	37.92230978139887	36.66154668091358	13.146298027692444
6	31.433555787426954	37.25111909603393	18.571251785033848	12.744073331505268
7	27.91833002738536	31.494941365421802	22.495922466320284	18.090806140872555
8	25.092794152343558	36.370633510953226	20.9613732377124	17.575199098990815
9	26.532740049549137	15.216927730823082	19.99479133045892	38.25554088916886
10	13.674625763932205	29.278142779357548	35.595244932458755	21.451986524251495
11	34.285238612882054	22.812874759286018	24.134431247279032	18.767455380552892
12	22.720534883617407	25.143002132234127	32.01809950722689	20.11836347692157
13	28.581717404667888	21.8176743714003	25.824100250117848	23.776507973813963
14	20.676636670591456	21.66060883945682	28.847469623687626	28.815284866264097
15	23.5898846694195	29.734883894850988	23.56958563255538	23.10564580317413
16	22.055670133381646	28.34058415846432	27.61440359756073	21.989342110593295
17	21.65730169513256	28.033347074362712	28.28340116297187	22.025950067532857
18	21.230206159087885	27.584489258476303	29.860015873800172	21.325288708635643
19	23.026183105884176	27.402440539182056	27.978583732202267	21.592792622731498
20	23.218847017114634	27.265798960283693	28.164666561608843	21.35068746099283
21	23.08877527761751	26.928854057596286	27.61483998261997	22.367530682166237
22	22.669531519915655	27.568528524464043	27.631571917552066	22.130368038068237
23	21.221762887947268	27.071033181583854	29.413830898307662	22.293373032161217
24	22.41904913707473	27.46516843327072	27.988983193849755	22.126799235804796
25	22.548996038646568	26.94833323093827	27.93096969907426	22.571701031340904
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	257.0
1	257.0
2	413.0
3	569.0
4	569.0
5	569.0
6	1243.5
7	1918.0
8	1918.0
9	1918.0
10	3132.5
11	4347.0
12	4347.0
13	4347.0
14	7732.0
15	11117.0
16	11117.0
17	11117.0
18	19496.0
19	27875.0
20	27875.0
21	27875.0
22	43548.0
23	59221.0
24	59221.0
25	59221.0
26	82294.5
27	105368.0
28	105368.0
29	105368.0
30	133590.0
31	161812.0
32	161812.0
33	161812.0
34	187249.5
35	212687.0
36	212687.0
37	212687.0
38	233659.5
39	254632.0
40	254632.0
41	254632.0
42	269506.0
43	284380.0
44	284380.0
45	284380.0
46	296129.0
47	307878.0
48	307878.0
49	307878.0
50	293581.0
51	279284.0
52	279284.0
53	279284.0
54	246621.0
55	213958.0
56	213958.0
57	213958.0
58	181019.5
59	148081.0
60	148081.0
61	148081.0
62	119275.0
63	90469.0
64	90469.0
65	90469.0
66	69148.0
67	47827.0
68	47827.0
69	47827.0
70	34802.5
71	21778.0
72	21778.0
73	21778.0
74	15227.0
75	8676.0
76	8676.0
77	8676.0
78	5930.5
79	3185.0
80	3185.0
81	3185.0
82	2156.5
83	1128.0
84	1128.0
85	1128.0
86	731.0
87	334.0
88	334.0
89	334.0
90	215.5
91	97.0
92	97.0
93	97.0
94	62.5
95	28.0
96	28.0
97	28.0
98	21.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10734689826656123
2	0.04940093576943738
3	0.03613834220250734
4	0.03195483953374418
5	0.03653889033036765
6	0.020694986606115656
7	0.020694986606115656
8	0.024522446494558557
9	0.029640561461662426
10	0.03613834220250734
11	0.021318061471676128
12	0.020071911740555184
13	0.016912032065212797
14	0.022964759330657374
15	0.02265322189787714
16	0.022697727245417174
17	0.02701474595680044
18	0.02011641708809522
19	0.0346251603861462
20	0.02398838232407815
21	0.029596056114122395
22	0.03778504006148859
23	0.03004110958952273
24	0.039965802090950235
25	0.03199934488128421
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2246921.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.63048909070565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.20365154524565	66.92031630862184
2	9.772392102753846	15.172711570458613
3	2.2516208558343394	5.243832848557592
4	0.7662738279907415	2.379448481773141
5	0.33626280429629624	1.3052122980266858
6	0.19098515994810697	0.8895762825502915
7	0.10828065811054127	0.5884116313729362
8	0.0765121341959051	0.4751739519201454
9	0.04725016774573272	0.3301248268547205
>10	0.2246195597250483	2.9544167632825853
>50	0.0124201889857284	0.6720299929996009
>100	0.00805307380083264	1.3312849053914653
>500	7.521716473386581E-4	0.44346062624374405
>1k	9.257497198006704E-4	1.2939995119465966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	4503	0.20040757997277164	No Hit
CCCATGTACTCTGCGTTGATACCAC	3879	0.1726362431077906	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3231	0.1437967779018488	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.450534754003367E-5	0.0
5	0.0	0.0	0.0	4.450534754003367E-5	0.0
6	0.0	0.0	0.0	4.450534754003367E-5	0.0
7	0.0	0.0	0.0	4.450534754003367E-5	0.0
8	0.0	0.0	0.0	4.450534754003367E-5	0.0
9	0.0	0.0	0.0	4.450534754003367E-5	0.0
10	0.0	0.0	0.0	4.450534754003367E-5	0.0
11	0.0	0.0	0.0	8.901069508006735E-5	0.0
12	0.0	0.0	0.0	8.901069508006735E-5	4.450534754003367E-5
13	0.0	0.0	0.0	8.901069508006735E-5	4.450534754003367E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGC	150	1.4551915E-11	12.031698	10
TATTAGG	205	0.0	11.124154	2
CAGCGTA	60	0.005882988	11.081827	9
GTGCGCC	180	3.6379788E-12	11.081581	11
TAGGACC	555	0.0	10.956149	4
GTATACG	70	0.0014775319	10.8697	1
GTAGGAC	1010	0.0	10.723702	3
TGTAGGA	1160	0.0	10.648661	2
AGGACGT	735	0.0	10.599322	5
GTATTAT	290	0.0	10.494884	1
GCGTGCG	145	1.4473699E-8	10.481334	9
GGACGTG	725	0.0	10.351008	6
GCGCCAC	175	2.3646862E-10	10.312884	13
GTCTAAC	130	3.7826794E-7	10.242602	1
AAGGCGT	205	3.6379788E-12	10.194417	6
GTCTAGG	190	9.822543E-11	10.011566	1
GTCCTAG	390	0.0	9.998732	1
CTAGGAC	325	0.0	9.939323	3
TAGGACG	815	0.0	9.909056	4
GTCCTAA	520	0.0	9.876795	1
>>END_MODULE