FastQCFastQC Report
Thu 26 May 2016
SRR1513024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246921
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC55010.24482391681772522No Hit
CCCATGTACTCTGCGTTGATACCAC51300.22831243288037273No Hit
GTATCAACGCAGAGTACTTTTTTTT47010.20921963878569827No Hit
TATCAACGCAGAGTACTTTTTTTTT32130.1429956816461282No Hit
GGTATCAACGCAGAGTACTTTTTTT26930.11985290092531067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG801.9553008E-613.0891295
AGACCGT604.0235583E-412.6924896
TAGGACC5250.012.6924894
GGCGAGG3850.011.82735219
AATCCCG907.5834832E-611.59463819
GTCCTAA5750.011.1037881
CGCAGTC600.00590805311.07549812
GGACGTG8100.011.0471676
TAGGACG8600.010.958344
TAAGACC1752.0008883E-1110.8792754
GTAGGAC10250.010.8660083
GACGTGA4200.010.842227
AGGACGT8450.010.8149015
GCGCCAC1158.901952E-710.73153813
GGACCGC803.701752E-410.7092886
CGGTACT803.701752E-410.7092884
TGGCGAG8550.010.21203918
TATGGCG8650.010.20665216
GGACCGT750.002603985410.153996
GTCCTAC10900.010.1413491
Produced by FastQC (version 0.11.5)