FastQCFastQC Report
Thu 26 May 2016
SRR1513023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071777
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58640.28304204554833845No Hit
CCCATGTACTCTGCGTTGATACCAC49300.2379599734913555No Hit
GTATCAACGCAGAGTACTTTTTTTT31540.1522364617427455No Hit
GAGTACATGGGAAGCAGTGGTATCA22870.11038832847357608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4300.013.2569764
TCCAACG604.0941886E-412.66624818
GTAGGAC8000.012.3498913
TAATACG658.0195E-411.6933344
GAACCGT855.327711E-511.1758326
GGACGTG5450.010.9810246
CGTCTGG700.001492777310.8562610
AGGACGT6300.010.7070295
TAGGACG6050.010.6786224
TAGGACT3300.010.652454
CCGCTCT909.525515E-510.5546979
TGTAGGA9700.010.2844062
AGAAGCG750.002646812710.1339775
ACTGCGT750.002649464610.1327548
TGTGCGA750.002649995310.13250910
AAGACGG856.591064E-410.0594635
GTCCTAA5200.010.05940151
ATAACGC856.595454E-410.0587353
GCCTAAG1803.8562575E-1010.0390791
AGGACCT7200.010.0284155
Produced by FastQC (version 0.11.5)