##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513023_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2071777
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.38797949779344	31.0	30.0	34.0	25.0	34.0
2	29.110989261875194	31.0	30.0	34.0	16.0	34.0
3	29.467018892477327	31.0	30.0	34.0	23.0	34.0
4	32.33181370388802	35.0	33.0	37.0	19.0	37.0
5	32.589225577849355	35.0	33.0	37.0	24.0	37.0
6	33.038909593069135	37.0	35.0	37.0	27.0	37.0
7	33.03179154899393	37.0	35.0	37.0	27.0	37.0
8	33.003084308784196	37.0	35.0	37.0	26.0	37.0
9	34.39190463066247	39.0	35.0	39.0	27.0	39.0
10	33.914135546441535	39.0	34.0	39.0	22.0	39.0
11	34.26649441518079	39.0	35.0	39.0	26.0	39.0
12	34.11087776338863	39.0	35.0	39.0	25.0	39.0
13	34.18573524081018	39.0	35.0	39.0	25.0	39.0
14	34.52061491174002	40.0	34.0	41.0	17.0	41.0
15	35.05159194256911	40.0	34.0	41.0	25.0	41.0
16	34.642479378813455	40.0	34.0	41.0	17.0	41.0
17	34.19478351193203	39.0	33.0	41.0	17.0	41.0
18	33.60808571578891	39.0	32.0	41.0	10.0	41.0
19	34.50841186092905	39.0	34.0	41.0	18.0	41.0
20	34.765373396847245	40.0	34.0	41.0	19.0	41.0
21	34.78786471710034	40.0	35.0	41.0	18.0	41.0
22	34.379306267035496	40.0	34.0	41.0	11.0	41.0
23	32.71463579333104	39.0	31.0	41.0	8.0	41.0
24	33.81327913187568	39.0	33.0	41.0	2.0	41.0
25	33.54851318457537	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5832684906701289
1101	2	0.9637481993255719
1101	3	0.6828991371370066
1101	4	0.6183579179567573
1101	5	0.8475410201140932
1101	6	0.5429427262599376
1101	7	0.6402303371896494
1101	8	0.6391995142997828
1101	9	0.7783822335125592
1101	10	0.9891834748954409
1101	11	0.6796476581600785
1101	12	0.6539310217181225
1101	13	0.6332736760034265
1101	14	-0.17415931530211282
1101	15	0.41467029852959314
1101	16	0.9644902375688957
1101	17	1.6948311790398378
1101	18	-4.539748985782296
1101	19	-0.8952350767983432
1101	20	0.11448908478584485
1101	21	0.5978044157454931
1101	22	1.1227661696119071
1101	23	2.9443518940504703
1101	24	1.9119379767309255
1101	25	2.1030419968089973
1102	1	0.9564100008977867
1102	2	1.2962356083114486
1102	3	1.0579236251286588
1102	4	1.5851176003220289
1102	5	1.3582077976114064
1102	6	1.0998750118939213
1102	7	1.0578255972425339
1102	8	1.0681444919261835
1102	9	1.1655270416245571
1102	10	1.5478717336322845
1102	11	1.0720864119528173
1102	12	1.1762569078101777
1102	13	1.1216865938805327
1102	14	2.0151448141550787
1102	15	1.4446228195670727
1102	16	1.7222527039489748
1102	17	1.6012108884765794
1102	18	1.975612525571151
1102	19	1.5746192219210613
1102	20	1.4649893661329472
1102	21	1.4474012389947504
1102	22	1.8719622270805587
1102	23	3.421323497664382
1102	24	2.2250237340307706
1102	25	2.628093489683728
1103	1	0.8004925108724805
1103	2	1.0389452918566775
1103	3	0.851902162738579
1103	4	1.285877381523136
1103	5	1.087106593190562
1103	6	0.7549644823271251
1103	7	0.664931497866533
1103	8	0.5656221267375017
1103	9	0.7370955944643285
1103	10	1.0247458162777576
1103	11	0.7758396966512677
1103	12	0.8881399135065209
1103	13	0.713194958788641
1103	14	1.4932196398226978
1103	15	0.9649385133068975
1103	16	1.2141648041805553
1103	17	1.6972267261405207
1103	18	1.6924641582138804
1103	19	1.1383955780826724
1103	20	1.2310813449230125
1103	21	1.1312881662081793
1103	22	1.5249848831840325
1103	23	2.9043488158597555
1103	24	1.9739379357154618
1103	25	2.044250686159657
1104	1	0.6660972622848327
1104	2	0.9839315359903829
1104	3	0.7928622799299418
1104	4	1.4002648788454906
1104	5	1.1951460226958162
1104	6	0.7248683540962801
1104	7	0.6325917100675369
1104	8	0.7043207232770641
1104	9	0.9183192465846304
1104	10	1.1736868239301046
1104	11	0.730695150319761
1104	12	0.9367931615031182
1104	13	0.6955265442440179
1104	14	1.5812592068765028
1104	15	1.0423998889663508
1104	16	1.4372471093890553
1104	17	1.8396075782497405
1104	18	2.3996533354085727
1104	19	1.5916992616125896
1104	20	0.97346029562582
1104	21	1.0183122608707151
1104	22	1.3605288037505545
1104	23	2.839244761393765
1104	24	1.7916731624450222
1104	25	1.7837448594615282
1105	1	0.4871594637558445
1105	2	0.8832917070819732
1105	3	0.6399068209153427
1105	4	0.8120053883637155
1105	5	0.8602449771753058
1105	6	0.5004847437860818
1105	7	0.4070287864999713
1105	8	0.3606927596520606
1105	9	0.47729490861756574
1105	10	0.9638719966980318
1105	11	0.7102164687351191
1105	12	0.6093630605664515
1105	13	0.642230211741591
1105	14	1.4696577445672716
1105	15	0.764521531354653
1105	16	1.2270311391936204
1105	17	1.599771729244189
1105	18	1.5760705601658813
1105	19	1.1402273649514072
1105	20	0.8849782747122674
1105	21	0.8720205589618928
1105	22	1.26789967826182
1105	23	2.7208803345792205
1105	24	1.7339023017365278
1105	25	1.696233541779435
1106	1	-0.5485988112655029
1106	2	0.0047027923873521615
1106	3	-0.3161267883101573
1106	4	-2.6027276916948274
1106	5	-0.47547075326141197
1106	6	-0.3726828749028499
1106	7	-0.4549229039747189
1106	8	-0.3895531918869608
1106	9	-0.29308868013272615
1106	10	-0.20686699376979334
1106	11	-0.13479958761104882
1106	12	-0.19045420695528748
1106	13	-0.2042225961511761
1106	14	0.5321395983553856
1106	15	-0.16053792644600406
1106	16	-1.188136353953908
1106	17	-5.912368422780467
1106	18	-6.797607792810698
1106	19	-2.7674857590067745
1106	20	-1.4626808265906703
1106	21	-0.9257318514827517
1106	22	-1.308600964888754
1106	23	-5.217044625541458
1106	24	-2.210224204137514
1106	25	-0.6733118090539705
1107	1	0.5750017742133693
1107	2	0.8975606573815824
1107	3	0.6296332460336878
1107	4	0.9816763385273575
1107	5	0.9907229219133598
1107	6	0.44353692171346637
1107	7	0.4301597857020525
1107	8	0.38415733273816244
1107	9	0.38891073319314273
1107	10	0.782711621808744
1107	11	0.548572284601569
1107	12	0.49402218638331874
1107	13	0.4331596021237658
1107	14	1.225473476196683
1107	15	0.7655090602751429
1107	16	-5.829086407466757
1107	17	-1.025357477672351
1107	18	0.566704491139582
1107	19	0.621254579919551
1107	20	0.42030398014303216
1107	21	0.4020659939589919
1107	22	0.5807555810847376
1107	23	2.5749219230765448
1107	24	1.5038210633993359
1107	25	1.113158429684546
1108	1	0.3874461921882748
1108	2	0.6854312162228844
1108	3	0.3907136770303268
1108	4	0.9085365892984711
1108	5	0.6531995676167952
1108	6	0.3109049777426449
1108	7	0.35111847177131494
1108	8	0.4052780700086842
1108	9	0.4376981348889615
1108	10	0.8886276913898996
1108	11	0.41862710536064185
1108	12	0.5474593144556366
1108	13	0.4500702741607583
1108	14	0.9696431390975278
1108	15	0.7559959727434773
1108	16	0.9025931134687752
1108	17	1.030319261400244
1108	18	0.8115211100810313
1108	19	0.7287121051181771
1108	20	0.5035039678475073
1108	21	0.6620179261337924
1108	22	1.0325054362760895
1108	23	2.6492420428810846
1108	24	1.4040525651154283
1108	25	1.590967693403428
1109	1	0.3036292172334001
1109	2	0.6538366517369667
1109	3	0.4120025530048572
1109	4	0.45756458937039923
1109	5	0.2090650256763169
1109	6	0.16309596467822018
1109	7	0.14166935849232232
1109	8	0.01544348841019172
1109	9	0.1886224316217664
1109	10	0.6330512919778641
1109	11	0.2819128711625609
1109	12	0.4306904108814038
1109	13	0.2170103508076764
1109	14	-0.9330597580197448
1109	15	-0.1401077930641179
1109	16	-0.3496964032808165
1109	17	0.8233179656527341
1109	18	1.5411713907632247
1109	19	0.7896266245889336
1109	20	0.5656211000146953
1109	21	0.4814627518041661
1109	22	0.20626536615996116
1109	23	-4.635516502285039
1109	24	-1.6060670430747592
1109	25	-0.30477631080656664
1110	1	0.4765172958850563
1110	2	0.8285608011283294
1110	3	0.6771241092314852
1110	4	1.1750324413706394
1110	5	0.840249658666572
1110	6	0.5382207587290395
1110	7	0.4276269699074149
1110	8	0.4225890320997294
1110	9	0.46523458544719176
1110	10	0.8722871095955185
1110	11	0.5077396081779497
1110	12	0.5818388793216513
1110	13	0.35806662273946444
1110	14	1.3605015720229332
1110	15	0.8251828619532091
1110	16	1.250217850716389
1110	17	1.4964279986893416
1110	18	2.0672684679401243
1110	19	1.299652751604583
1110	20	0.9637597748050126
1110	21	0.7198800340563025
1110	22	1.0956209888600483
1110	23	2.4461737928002236
1110	24	1.5034235625324612
1110	25	1.7710686733083492
1111	1	0.32273283163429056
1111	2	0.6384228705505173
1111	3	0.37207594352349815
1111	4	0.9099681039333092
1111	5	0.5935594186510329
1111	6	0.10167594287927528
1111	7	-0.03572213785162859
1111	8	-0.17995678289509698
1111	9	-0.017752725851977402
1111	10	0.28144749665754887
1111	11	0.061928880685570675
1111	12	0.15195745238357716
1111	13	0.00240179029123766
1111	14	-0.541070131718044
1111	15	-0.20575246805160674
1111	16	0.25510578901954517
1111	17	0.7923354217926999
1111	18	1.3656793637715268
1111	19	0.47644845366217226
1111	20	0.24757711961012063
1111	21	0.058095235776185916
1111	22	0.38176191613729316
1111	23	0.6030794817467893
1111	24	0.3550783873436316
1111	25	0.035425037744673205
1112	1	0.13094524725247325
1112	2	0.4262090231739357
1112	3	0.1925441712266469
1112	4	0.7708574481184698
1112	5	0.5207178052952841
1112	6	-0.15052679715017092
1112	7	-0.21437091405287845
1112	8	-0.17481017966589718
1112	9	-0.10171399164800476
1112	10	0.11664779806513081
1112	11	-0.12394853821161433
1112	12	0.02784723449282467
1112	13	-0.17975843129110558
1112	14	0.409820120888142
1112	15	-0.04158495449043187
1112	16	0.29604879519101956
1112	17	0.4442383466912503
1112	18	0.5297754884691415
1112	19	0.045930718822667416
1112	20	0.008381358136148265
1112	21	0.0013853340593783514
1112	22	0.07175826435715038
1112	23	1.7002763695180718
1112	24	0.5242946950573213
1112	25	0.589888045103244
1113	1	0.6742055195869625
1113	2	1.060904684370719
1113	3	0.7435045329617544
1113	4	0.8884386962790458
1113	5	0.9701419296621481
1113	6	0.4688382180643771
1113	7	0.4139505181158398
1113	8	0.40007474485926764
1113	9	0.5807998717929337
1113	10	0.9199515185803762
1113	11	0.5675752770916986
1113	12	0.5299068984777122
1113	13	0.48913468577310937
1113	14	1.1598538371554525
1113	15	0.6851739465371054
1113	16	1.1418662933483574
1113	17	1.4209235813613859
1113	18	2.136024004305419
1113	19	1.216858296459236
1113	20	0.9689246114373233
1113	21	0.8040435969668991
1113	22	1.0510280106722902
1113	23	0.968855641534482
1113	24	0.8889008457088394
1113	25	1.3431490494091847
1114	1	-0.5401417782584055
1114	2	-3.412837229487959
1114	3	-1.2420315123355934
1114	4	-0.079525184083451
1114	5	0.1263540832656247
1114	6	-0.01668207943700395
1114	7	-0.21285223062235303
1114	8	-0.16815042574918948
1114	9	-0.014106024129091566
1114	10	0.04180083768614651
1114	11	-0.1872780792562878
1114	12	-0.10507089185138341
1114	13	-0.16284606144660785
1114	14	0.6611059188997146
1114	15	0.06591131288978147
1114	16	0.4112107011652455
1114	17	0.6614351823843805
1114	18	0.808994100208615
1114	19	0.21247513058919765
1114	20	0.02055283676629216
1114	21	0.025861684410273256
1114	22	0.11515884230547613
1114	23	1.7538522148492675
1114	24	0.7027201085184416
1114	25	0.6162387053946219
1115	1	0.07137338270207039
1115	2	0.28156965275084644
1115	3	0.05826009621283035
1115	4	0.2945860721969993
1115	5	0.23744689669069885
1115	6	-0.1359765564507498
1115	7	-0.33263251985855646
1115	8	-0.2536691348445501
1115	9	-0.18736799648688418
1115	10	-0.20189827908974678
1115	11	-0.13133123028232774
1115	12	-0.2977613703301003
1115	13	-0.5143287367991931
1115	14	0.36954299924704515
1115	15	-0.30802937663283103
1115	16	0.19874590761595812
1115	17	0.7194032907297654
1115	18	1.0446454098063285
1115	19	0.2640553839803985
1115	20	-0.17001776531704849
1115	21	-0.09239363411231949
1115	22	0.13314662210638772
1115	23	1.9200489138197199
1115	24	0.612948745897846
1115	25	0.2335241311145353
1116	1	0.33313656345747944
1116	2	0.22996020668529837
1116	3	0.35888947481991096
1116	4	0.9567638612330356
1116	5	0.48404954269662426
1116	6	0.23129784442479462
1116	7	0.14501656434584476
1116	8	0.16874914196142754
1116	9	0.10753748011727993
1116	10	0.6656617599676338
1116	11	0.23530985004870075
1116	12	0.32614655854203534
1116	13	0.16905476124127716
1116	14	0.8595508839921067
1116	15	0.22304618665306464
1116	16	0.8075544757345909
1116	17	1.0286165209160316
1116	18	1.5487320154770998
1116	19	0.7645848479613164
1116	20	0.4893562880849771
1116	21	0.34572408292984136
1116	22	0.8085225049850777
1116	23	2.1391969662894326
1116	24	1.075637698927089
1116	25	1.3639139365159636
1201	1	1.0709633314395326
1201	2	1.4657580986961634
1201	3	1.1208508527013095
1201	4	1.7812978749884607
1201	5	1.5544899716848235
1201	6	1.0964270916383327
1201	7	1.0176674191099053
1201	8	1.0300571100674034
1201	9	1.216767930085183
1201	10	1.6766596039029906
1201	11	1.2330990242785163
1201	12	1.2412475728228785
1201	13	1.2233316100745668
1201	14	2.104621692034925
1201	15	1.6354094778787385
1201	16	1.8834676842043052
1201	17	2.326808765616555
1201	18	2.885857136273003
1201	19	1.9377770569480646
1201	20	1.739245027759054
1201	21	1.7227524470035291
1201	22	2.045776757281878
1201	23	3.5439486512417133
1201	24	2.411110652691846
1201	25	2.5861687826138606
1202	1	1.1609388888620877
1202	2	1.4557723688782787
1202	3	1.1938801055450519
1202	4	1.696093150384442
1202	5	1.5733531756186991
1202	6	1.2425901011847387
1202	7	1.088687932754091
1202	8	1.0511143802412803
1202	9	1.1820846951810964
1202	10	1.6932786003364768
1202	11	1.3390238268022543
1202	12	1.4089947990948417
1202	13	1.2884246442488774
1202	14	2.1214530938486575
1202	15	1.5716862164522993
1202	16	1.9226782527876622
1202	17	2.2762865552003504
1202	18	2.8078198319989482
1202	19	2.085171976516129
1202	20	1.799342262679609
1202	21	1.8453165289757365
1202	22	2.089653963703256
1202	23	3.7969200614569374
1202	24	2.5221352435309186
1202	25	2.7377754650950976
1203	1	0.9393537251994566
1203	2	1.2485589836719342
1203	3	0.9619137737547767
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2211	16	-0.8991258319324587
2211	17	-0.3287272156984429
2211	18	-5.452827400244349
2211	19	-2.2134460846964856
2211	20	-1.4652655576392561
2211	21	-1.3567466813892892
2211	22	-0.987843223930092
2211	23	-5.252853009647449
2211	24	-2.732906166143618
2211	25	-1.9733096235429386
2212	1	0.3723200077432054
2212	2	0.6184821590880674
2212	3	0.4430234621884068
2212	4	0.9266292809205723
2212	5	0.7676305648960877
2212	6	0.3849269310215675
2212	7	0.2609664875326487
2212	8	0.21489060401750493
2212	9	0.012370242966646572
2212	10	-3.5257132411553656
2212	11	-0.9265647512863211
2212	12	-0.12427443167439378
2212	13	0.19098874681297673
2212	14	1.0519426043381657
2212	15	0.47035754512362615
2212	16	0.6798752224846325
2212	17	1.2040954130092132
2212	18	1.7549876641667836
2212	19	1.0663957527399077
2212	20	0.5963935447308941
2212	21	0.5872454334046395
2212	22	0.7700502340995214
2212	23	2.159344303881177
2212	24	1.2796468985425733
2212	25	1.2093896842093201
2213	1	-1.3101972109410376
2213	2	-3.470187993029622
2213	3	-1.4709073108215946
2213	4	-3.3073200317225293
2213	5	-1.219923408610775
2213	6	-0.9230432572484091
2213	7	-0.7517638442340839
2213	8	-0.6501861037144465
2213	9	-0.6537920529815153
2213	10	-0.7398155984697112
2213	11	-0.7342688958213017
2213	12	-0.5659048997074265
2213	13	-0.9843299031309414
2213	14	-6.3542263658779135
2213	15	-2.7629817208922987
2213	16	-1.9518437722964634
2213	17	-0.7297472040453954
2213	18	0.13043534995708228
2213	19	-0.5497440175540405
2213	20	-0.6253694816788169
2213	21	-0.7627874744441101
2213	22	-0.7621630273000335
2213	23	-5.7091980174662815
2213	24	-2.9005229244966237
2213	25	-1.9919894790970396
2214	1	-0.01074406538030459
2214	2	0.14552119731581215
2214	3	-0.07509396768236698
2214	4	0.24245844927574467
2214	5	0.013840659902619734
2214	6	-0.18291043220956027
2214	7	-0.18797178073697296
2214	8	-0.39872369649129524
2214	9	-0.22175241001187374
2214	10	0.2055290480066887
2214	11	-0.14209449148173547
2214	12	-0.1286280720904358
2214	13	-0.14390476482644488
2214	14	0.4289910981920073
2214	15	0.05193348280654675
2214	16	0.39078746526909214
2214	17	0.6689619085740688
2214	18	1.205277636700437
2214	19	0.19198132615608898
2214	20	0.13749805191266518
2214	21	0.07799693054543866
2214	22	0.44259334725185084
2214	23	1.8787922754242459
2214	24	0.8890547962417088
2214	25	0.6962616730426774
2215	1	-2.365704831680052
2215	2	-4.366521172379645
2215	3	-2.335794533067581
2215	4	-4.010759114911096
2215	5	-4.733642855321921
2215	6	-2.4375378220500465
2215	7	-1.7647159303133577
2215	8	-2.0349758707566217
2215	9	-1.8058933960621673
2215	10	-3.318830695380999
2215	11	-2.1687251414678173
2215	12	-1.9240721449549554
2215	13	-1.8947778905367727
2215	14	-1.2682826028791254
2215	15	-1.8917228898327991
2215	16	-2.632199349088623
2215	17	-7.008724607779147
2215	18	-3.295026727773731
2215	19	-2.3909116217970237
2215	20	-2.2082355962834725
2215	21	-2.2441956262686062
2215	22	-2.058621976129416
2215	23	-6.408444934145525
2215	24	-3.8399165459285207
2215	25	-3.5325718839791627
2216	1	-1.112604120896755
2216	2	-0.6944179747222492
2216	3	-0.9404342146096134
2216	4	-3.28925819097471
2216	5	-1.4687563744887484
2216	6	-1.1994500317573191
2216	7	-1.1955607251165254
2216	8	-1.062552617625176
2216	9	-1.0223311415605139
2216	10	-0.7351811858471038
2216	11	-1.3248616281353165
2216	12	-2.0321416590700068
2216	13	-1.376483967715913
2216	14	-7.206410436433018
2216	15	-3.5149737491953523
2216	16	-1.9762591157914926
2216	17	-0.9135005526552433
2216	18	-0.30677471783891264
2216	19	-0.7842164977311583
2216	20	-1.422333800818322
2216	21	-1.6059011116493807
2216	22	-0.9565522582314117
2216	23	0.09385607827880449
2216	24	-0.5809207484894756
2216	25	-0.46864066433469986
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93868.0
3	1.0
4	799.0
5	4785.0
6	5888.0
7	5346.0
8	4897.0
9	4546.0
10	4268.0
11	4321.0
12	4152.0
13	4147.0
14	4231.0
15	4413.0
16	4391.0
17	4777.0
18	5472.0
19	6205.0
20	7764.0
21	9967.0
22	12007.0
23	14481.0
24	17243.0
25	20667.0
26	23317.0
27	26751.0
28	30000.0
29	36294.0
30	42828.0
31	48799.0
32	63381.0
33	83149.0
34	107935.0
35	143174.0
36	203488.0
37	285940.0
38	728085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58181638946904	18.10904792785549	12.133940143094478	25.175195539581
2	14.183117635696348	20.039646513424763	41.547904191616766	24.229331659262122
3	19.00768345683614	24.703764666914203	29.204040610833125	27.084511265416527
4	10.689347492513706	16.470379765904212	39.55201973040647	33.288253011175605
5	12.225220670850975	38.074165492153575	36.4657223243433	13.23489151265215
6	32.0397179883789	36.71809072493826	18.29981017705865	12.942381109624195
7	28.244848972884302	31.43638815226349	22.130093758614414	18.188669116237797
8	25.633015129987346	35.85704167927942	20.628035206585952	17.881907984147283
9	26.746143673712965	15.00960551021699	19.657716490915682	38.58653432515437
10	13.813884138221407	29.14318023576401	35.39395882483026	21.648976801184325
11	34.51519729032065	22.748889754652556	23.700376896855204	19.035536058171594
12	22.8601401268412	25.09296811976775	31.69789259388175	20.3489991595093
13	28.800413226132694	21.592899848178014	25.563298181756743	24.04338874393255
14	20.780230215563375	21.51119818206659	28.6812113569664	29.02736024540363
15	24.08756410671103	29.18549294639103	23.476851581407495	23.250091365490448
16	22.328900449854153	28.04805396318708	27.442213680239536	22.18083190671923
17	21.872198213326136	27.889705743901068	28.071675526971262	22.166420515801533
18	21.55324151182045	27.30304478591877	29.526747481184312	21.616966221076463
19	23.17593395976343	27.281817738487884	27.939004423251205	21.603243878497473
20	23.43939868673941	26.947719174077754	28.00196781492884	21.610914324253994
21	23.25033458929044	26.75468666413029	27.526318127304222	22.468660619275045
22	22.841858706657916	27.31885663959348	27.36144367140773	22.477840982340865
23	21.35265336628013	26.814367198431064	29.208079497184748	22.624899938104054
24	22.551034844738282	27.383320127069847	27.693751819784477	22.371893208407393
25	22.718002497168236	26.69985602173869	27.679362053664974	22.9027794274281
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	290.0
2	429.5
3	569.0
4	569.0
5	569.0
6	1184.0
7	1799.0
8	1799.0
9	1799.0
10	2804.5
11	3810.0
12	3810.0
13	3810.0
14	6587.5
15	9365.0
16	9365.0
17	9365.0
18	16861.0
19	24357.0
20	24357.0
21	24357.0
22	37613.5
23	50870.0
24	50870.0
25	50870.0
26	71189.0
27	91508.0
28	91508.0
29	91508.0
30	115871.5
31	140235.0
32	140235.0
33	140235.0
34	163450.0
35	186665.0
36	186665.0
37	186665.0
38	206333.5
39	226002.0
40	226002.0
41	226002.0
42	242941.5
43	259881.0
44	259881.0
45	259881.0
46	275181.5
47	290482.0
48	290482.0
49	290482.0
50	278334.0
51	266186.0
52	266186.0
53	266186.0
54	235717.5
55	205249.0
56	205249.0
57	205249.0
58	174525.5
59	143802.0
60	143802.0
61	143802.0
62	116159.0
63	88516.0
64	88516.0
65	88516.0
66	68098.0
67	47680.0
68	47680.0
69	47680.0
70	34504.5
71	21329.0
72	21329.0
73	21329.0
74	14942.0
75	8555.0
76	8555.0
77	8555.0
78	5824.5
79	3094.0
80	3094.0
81	3094.0
82	2074.0
83	1054.0
84	1054.0
85	1054.0
86	699.0
87	344.0
88	344.0
89	344.0
90	218.5
91	93.0
92	93.0
93	93.0
94	63.5
95	34.0
96	34.0
97	34.0
98	21.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11463588986652522
2	0.04715758501035585
3	0.03398049114359316
4	0.03180844270401689
5	0.034077026629796545
6	0.01800386817693217
7	0.019017490782067763
8	0.02220316182677962
9	0.027512613567966047
10	0.034897578262525354
11	0.01872788432345759
12	0.017762529461423694
13	0.013128826123660993
14	0.01998284564410166
15	0.020562058561321993
16	0.02118953922164403
17	0.026740329678338934
18	0.017714261718322
19	0.0326289943367457
20	0.021430877937152502
21	0.028043558742084692
22	0.03494584600562706
23	0.02698166839384741
24	0.03813151705033891
25	0.030456945897169435
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2071777.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.62094057278892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.54596838563685	63.93445650959138
2	10.687105435515594	16.163379300685087
3	2.70408118804057	6.134554884744373
4	0.9349611715578019	2.8281057276895063
5	0.4051606763031434	1.5319315712575492
6	0.20538957154244572	0.9319051550329123
7	0.12697231279325752	0.6721236004089947
8	0.0805117606561293	0.4870700054390209
9	0.05831158491328976	0.3968619207689719
>10	0.2294974063560629	2.903337056266482
>50	0.011522935223917513	0.5975051666824684
>100	0.008454151209835715	1.3150872537916154
>500	0.0012896376568814893	0.719431690234748
>1k	7.093007112846994E-4	1.0983109252301433
>5k	6.448188284406358E-5	0.2859392321767697
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5864	0.28304204554833845	No Hit
CCCATGTACTCTGCGTTGATACCAC	4930	0.2379599734913555	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3154	0.1522364617427455	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2287	0.11038832847357608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.8267743101694826E-5	0.0	0.0	0.0
6	0.0	4.8267743101694826E-5	0.0	0.0	0.0
7	0.0	4.8267743101694826E-5	0.0	0.0	4.8267743101694826E-5
8	0.0	4.8267743101694826E-5	0.0	0.0	4.8267743101694826E-5
9	0.0	4.8267743101694826E-5	0.0	0.0	4.8267743101694826E-5
10	0.0	4.8267743101694826E-5	0.0	0.0	4.8267743101694826E-5
11	0.0	4.8267743101694826E-5	0.0	4.8267743101694826E-5	4.8267743101694826E-5
12	0.0	4.8267743101694826E-5	0.0	4.8267743101694826E-5	4.8267743101694826E-5
13	0.0	4.8267743101694826E-5	0.0	4.8267743101694826E-5	4.8267743101694826E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	430	0.0	13.256976	4
TCCAACG	60	4.0941886E-4	12.666248	18
GTAGGAC	800	0.0	12.349891	3
TAATACG	65	8.0195E-4	11.693334	4
GAACCGT	85	5.327711E-5	11.175832	6
GGACGTG	545	0.0	10.981024	6
CGTCTGG	70	0.0014927773	10.85626	10
AGGACGT	630	0.0	10.707029	5
TAGGACG	605	0.0	10.678622	4
TAGGACT	330	0.0	10.65245	4
CCGCTCT	90	9.525515E-5	10.554697	9
TGTAGGA	970	0.0	10.284406	2
AGAAGCG	75	0.0026468127	10.133977	5
ACTGCGT	75	0.0026494646	10.132754	8
TGTGCGA	75	0.0026499953	10.132509	10
AAGACGG	85	6.591064E-4	10.059463	5
GTCCTAA	520	0.0	10.0594015	1
ATAACGC	85	6.595454E-4	10.058735	3
GCCTAAG	180	3.8562575E-10	10.039079	1
AGGACCT	720	0.0	10.028415	5
>>END_MODULE