FastQCFastQC Report
Thu 26 May 2016
SRR1513023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071777
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC70360.33961184046352483No Hit
CCCATGTACTCTGCGTTGATACCAC65940.31827749801257565No Hit
GTATCAACGCAGAGTACTTTTTTTT51300.24761352211169446No Hit
TATCAACGCAGAGTACTTTTTTTTT33880.16353111362854206No Hit
GGTATCAACGCAGAGTACTTTTTTT30740.1483750422946099No Hit
GAGTACATGGGAAGCAGTGGTATCA24640.11893171900257606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGA3550.011.48986419
CTTAGAC1751.8189894E-1211.4246253
ACCGTGC1105.061229E-711.2115958
TGGCGAG5900.011.09947918
GGTCGGT600.005943555411.06651911
TAGGACC4150.011.0116874
GTAGGAC8300.010.6675723
GGCGTGC1701.4188117E-1010.602828
GTCCTAC7950.010.5466161
AAGGCGT1658.949428E-1010.38602356
CCCTTAC1855.638867E-1110.3004421
GACCGTG750.00267908110.1191847
GGACCGT856.465357E-410.0805526
AGGACCG951.6089978E-410.0216025
GTGCGCC1901.0186341E-109.9848311
TCCTACA9550.09.9754162
ATGGCGA6700.09.77629317
TGTAGGA9750.09.7707932
TATGGCG6650.09.70704716
GGACGTG6500.09.6669916
Produced by FastQC (version 0.11.5)