FastQCFastQC Report
Thu 26 May 2016
SRR1513022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1732075
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC58180.3358976949612459No Hit
CCCATGTACTCTGCGTTGATACCAC47360.2734292683630905No Hit
GTATCAACGCAGAGTACTTTTTTTT24810.1432386010998369No Hit
GAGTACATGGGAAGCAGTGGTATCA23590.13619502619690257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG655.447669E-513.1552665
CGTTTAT957.408926E-812.99727314
TAGGACC3900.012.6687664
GAAGCGT701.0917046E-412.2134886
GTAGGAC8800.011.7683153
TCCAACG1157.069502E-811.56579818
AGGACGT6750.011.5419865
GACGTGG3300.011.5134017
GTATTAG2700.011.2738271
TGTAGGA9750.011.1098542
ATAGGAC1201.2734563E-711.0845313
GCGTTTA1201.2790588E-711.08100913
GGACGTG6900.011.0137736
TGGCGAG8300.010.98850318
TAGGACG7150.010.8969114
CGTGGAA3700.010.7821459
GGCGAGA4950.010.55608619
CCAACGA909.510758E-510.55608519
GTTCTAA2350.010.5242381
TTAGGAC6250.010.4891323
Produced by FastQC (version 0.11.5)